YDL185W / VMA1

Subunit A of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase; protein precursor undergoes self-catalyzed splicing to yield the extein Tfp1p and the intein Vde (PI-SceI), which is a site-specific endonuclease

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL185W / VMA1

Click on Significant Values for Screen Details ID:SGTC_73|Compound:0327-0325|FD-Score:-7.28|P-value:1.63E-8 ID:SGTC_75|Compound:0159-0059|FD-Score:5.08|P-value:7.50E-5 ID:SGTC_77|Compound:0828-0286|FD-Score:4.23|P-value:8.28E-4 ID:SGTC_119|Compound:0121-0043|FD-Score:-4.35|P-value:4.55E-4 ID:SGTC_164|Compound:4100-3994|FD-Score:7.63|P-value:5.59E-9 ID:SGTC_176|Compound:1486-1328|FD-Score:4.20|P-value:8.94E-4 ID:SGTC_217|Compound:ketoconazole|FD-Score:-4.37|P-value:4.31E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.39|P-value:4.00E-4 ID:SGTC_344|Compound:k064-0035|FD-Score:-5.61|P-value:9.70E-6 ID:SGTC_346|Compound:4513-0042|FD-Score:-4.96|P-value:7.85E-5 ID:SGTC_391|Compound:gliotoxin|FD-Score:5.78|P-value:7.99E-6 ID:SGTC_487|Compound:nimodipine|FD-Score:4.40|P-value:5.21E-4 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:8.04|P-value:8.64E-10 ID:SGTC_505|Compound:gw-5074|FD-Score:4.66|P-value:2.61E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:-4.42|P-value:3.69E-4 ID:SGTC_551|Compound:k048-0028|FD-Score:-6.57|P-value:2.93E-7 ID:SGTC_690|Compound:glyoxal|FD-Score:-5.35|P-value:2.29E-5 ID:SGTC_717|Compound:1532-0246|FD-Score:5.46|P-value:2.31E-5 ID:SGTC_723|Compound:4182-0001|FD-Score:-4.56|P-value:2.50E-4 ID:SGTC_841|Compound:0251-0215|FD-Score:5.12|P-value:6.70E-5 ID:SGTC_939|Compound:1165-0479|FD-Score:5.14|P-value:6.41E-5 ID:SGTC_1143|Compound:3454-2408|FD-Score:-4.24|P-value:6.08E-4 ID:SGTC_1148|Compound:3909-7663|FD-Score:6.33|P-value:1.11E-6 ID:SGTC_1150|Compound:3909-7983|FD-Score:4.23|P-value:8.32E-4 ID:SGTC_1157|Compound:4092-0394|FD-Score:5.68|P-value:1.12E-5 ID:SGTC_1239|Compound:0337-0584|FD-Score:5.08|P-value:7.67E-5 ID:SGTC_1368|Compound:1852-0141|FD-Score:4.58|P-value:3.25E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-4.65|P-value:1.98E-4 ID:SGTC_1571|Compound:st070080|FD-Score:4.16|P-value:9.92E-4 ID:SGTC_1598|Compound:3(2'-chlorophenyl)-7-hydroxy-4-phenylcoumarin|FD-Score:4.32|P-value:6.59E-4 ID:SGTC_1600|Compound:coumarin 106|FD-Score:6.75|P-value:2.21E-7 ID:SGTC_1658|Compound:st012944|FD-Score:6.55|P-value:4.88E-7 ID:SGTC_1660|Compound:st012960|FD-Score:4.92|P-value:1.22E-4 ID:SGTC_1764|Compound:st044502|FD-Score:4.39|P-value:5.39E-4 ID:SGTC_1827|Compound:berberine|FD-Score:-6.11|P-value:1.70E-6 ID:SGTC_1829|Compound:berberine|FD-Score:-4.39|P-value:4.09E-4 ID:SGTC_1854|Compound:st056233|FD-Score:-4.22|P-value:6.33E-4 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:5.73|P-value:9.47E-6 ID:SGTC_1908|Compound:st060353|FD-Score:-4.81|P-value:1.24E-4 ID:SGTC_1975|Compound:st070967|FD-Score:4.87|P-value:1.41E-4 ID:SGTC_2126|Compound:5251174|FD-Score:-4.73|P-value:1.56E-4 ID:SGTC_2177|Compound:5811945|FD-Score:-4.35|P-value:4.55E-4 ID:SGTC_2181|Compound:6316240|FD-Score:4.56|P-value:3.44E-4 ID:SGTC_2302|Compound:7771753|FD-Score:4.90|P-value:1.31E-4 ID:SGTC_2307|Compound:7538926|FD-Score:5.28|P-value:4.07E-5 ID:SGTC_2339|Compound:9000433|FD-Score:5.22|P-value:4.89E-5 ID:SGTC_2340|Compound:7987427|FD-Score:5.04|P-value:8.56E-5 ID:SGTC_2421|Compound:5690543|FD-Score:-4.26|P-value:5.69E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:4.57|P-value:3.28E-4 ID:SGTC_2557|Compound:derrustone|FD-Score:-4.45|P-value:3.48E-4 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:4.53|P-value:3.74E-4 ID:SGTC_2666|Compound:tannic acid|FD-Score:4.74|P-value:2.08E-4 ID:SGTC_2736|Compound:butenafine|FD-Score:-4.08|P-value:9.29E-4 ID:SGTC_2740|Compound:felodipine|FD-Score:5.47|P-value:2.20E-5 ID:SGTC_2752|Compound:diclazuril|FD-Score:8.16|P-value:4.94E-10 ID:SGTC_2816|Compound:7982706|FD-Score:-4.80|P-value:1.26E-4 ID:SGTC_2841|Compound:9004761|FD-Score:6.06|P-value:2.95E-6 ID:SGTC_2858|Compound:9025781|FD-Score:4.17|P-value:9.69E-4 ID:SGTC_2913|Compound:7966556|FD-Score:-4.88|P-value:9.93E-5 ID:SGTC_2928|Compound:9011484|FD-Score:-5.92|P-value:3.38E-6 ID:SGTC_3129|Compound:9127257|FD-Score:4.27|P-value:7.50E-4 ID:SGTC_3140|Compound:9094243|FD-Score:-4.39|P-value:4.09E-4 ID:SGTC_3141|Compound:9094609|FD-Score:5.11|P-value:6.89E-5 ID:SGTC_3142|Compound:9086498|FD-Score:8.70|P-value:3.64E-11 ID:SGTC_3145|Compound:9085516|FD-Score:-5.51|P-value:1.37E-5 ID:SGTC_3155|Compound:9099265|FD-Score:4.81|P-value:1.67E-4 ID:SGTC_3174|Compound:9107204|FD-Score:4.90|P-value:1.32E-4 ID:SGTC_3202|Compound:9113761|FD-Score:4.45|P-value:4.56E-4 ID:SGTC_3207|Compound:9114163|FD-Score:-4.55|P-value:2.57E-4 ID:SGTC_3249|Compound:9135183|FD-Score:-4.07|P-value:9.52E-4 ID:SGTC_3266|Compound:9136860|FD-Score:-4.69|P-value:1.75E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:-7.28|P-value:1.63E-8 ID:SGTC_75|Compound:0159-0059|FD-Score:5.08|P-value:7.50E-5 ID:SGTC_77|Compound:0828-0286|FD-Score:4.23|P-value:8.28E-4 ID:SGTC_119|Compound:0121-0043|FD-Score:-4.35|P-value:4.55E-4 ID:SGTC_164|Compound:4100-3994|FD-Score:7.63|P-value:5.59E-9 ID:SGTC_176|Compound:1486-1328|FD-Score:4.20|P-value:8.94E-4 ID:SGTC_217|Compound:ketoconazole|FD-Score:-4.37|P-value:4.31E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.39|P-value:4.00E-4 ID:SGTC_344|Compound:k064-0035|FD-Score:-5.61|P-value:9.70E-6 ID:SGTC_346|Compound:4513-0042|FD-Score:-4.96|P-value:7.85E-5 ID:SGTC_391|Compound:gliotoxin|FD-Score:5.78|P-value:7.99E-6 ID:SGTC_487|Compound:nimodipine|FD-Score:4.40|P-value:5.21E-4 ID:SGTC_498|Compound:2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ)|FD-Score:8.04|P-value:8.64E-10 ID:SGTC_505|Compound:gw-5074|FD-Score:4.66|P-value:2.61E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:-4.42|P-value:3.69E-4 ID:SGTC_551|Compound:k048-0028|FD-Score:-6.57|P-value:2.93E-7 ID:SGTC_690|Compound:glyoxal|FD-Score:-5.35|P-value:2.29E-5 ID:SGTC_717|Compound:1532-0246|FD-Score:5.46|P-value:2.31E-5 ID:SGTC_723|Compound:4182-0001|FD-Score:-4.56|P-value:2.50E-4 ID:SGTC_841|Compound:0251-0215|FD-Score:5.12|P-value:6.70E-5 ID:SGTC_939|Compound:1165-0479|FD-Score:5.14|P-value:6.41E-5 ID:SGTC_1143|Compound:3454-2408|FD-Score:-4.24|P-value:6.08E-4 ID:SGTC_1148|Compound:3909-7663|FD-Score:6.33|P-value:1.11E-6 ID:SGTC_1150|Compound:3909-7983|FD-Score:4.23|P-value:8.32E-4 ID:SGTC_1157|Compound:4092-0394|FD-Score:5.68|P-value:1.12E-5 ID:SGTC_1239|Compound:0337-0584|FD-Score:5.08|P-value:7.67E-5 ID:SGTC_1368|Compound:1852-0141|FD-Score:4.58|P-value:3.25E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-4.65|P-value:1.98E-4 ID:SGTC_1571|Compound:st070080|FD-Score:4.16|P-value:9.92E-4 ID:SGTC_1598|Compound:3(2'-chlorophenyl)-7-hydroxy-4-phenylcoumarin|FD-Score:4.32|P-value:6.59E-4 ID:SGTC_1600|Compound:coumarin 106|FD-Score:6.75|P-value:2.21E-7 ID:SGTC_1658|Compound:st012944|FD-Score:6.55|P-value:4.88E-7 ID:SGTC_1660|Compound:st012960|FD-Score:4.92|P-value:1.22E-4 ID:SGTC_1764|Compound:st044502|FD-Score:4.39|P-value:5.39E-4 ID:SGTC_1827|Compound:berberine|FD-Score:-6.11|P-value:1.70E-6 ID:SGTC_1829|Compound:berberine|FD-Score:-4.39|P-value:4.09E-4 ID:SGTC_1854|Compound:st056233|FD-Score:-4.22|P-value:6.33E-4 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:5.73|P-value:9.47E-6 ID:SGTC_1908|Compound:st060353|FD-Score:-4.81|P-value:1.24E-4 ID:SGTC_1975|Compound:st070967|FD-Score:4.87|P-value:1.41E-4 ID:SGTC_2126|Compound:5251174|FD-Score:-4.73|P-value:1.56E-4 ID:SGTC_2177|Compound:5811945|FD-Score:-4.35|P-value:4.55E-4 ID:SGTC_2181|Compound:6316240|FD-Score:4.56|P-value:3.44E-4 ID:SGTC_2302|Compound:7771753|FD-Score:4.90|P-value:1.31E-4 ID:SGTC_2307|Compound:7538926|FD-Score:5.28|P-value:4.07E-5 ID:SGTC_2339|Compound:9000433|FD-Score:5.22|P-value:4.89E-5 ID:SGTC_2340|Compound:7987427|FD-Score:5.04|P-value:8.56E-5 ID:SGTC_2421|Compound:5690543|FD-Score:-4.26|P-value:5.69E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:4.57|P-value:3.28E-4 ID:SGTC_2557|Compound:derrustone|FD-Score:-4.45|P-value:3.48E-4 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:4.53|P-value:3.74E-4 ID:SGTC_2666|Compound:tannic acid|FD-Score:4.74|P-value:2.08E-4 ID:SGTC_2736|Compound:butenafine|FD-Score:-4.08|P-value:9.29E-4 ID:SGTC_2740|Compound:felodipine|FD-Score:5.47|P-value:2.20E-5 ID:SGTC_2752|Compound:diclazuril|FD-Score:8.16|P-value:4.94E-10 ID:SGTC_2816|Compound:7982706|FD-Score:-4.80|P-value:1.26E-4 ID:SGTC_2841|Compound:9004761|FD-Score:6.06|P-value:2.95E-6 ID:SGTC_2858|Compound:9025781|FD-Score:4.17|P-value:9.69E-4 ID:SGTC_2913|Compound:7966556|FD-Score:-4.88|P-value:9.93E-5 ID:SGTC_2928|Compound:9011484|FD-Score:-5.92|P-value:3.38E-6 ID:SGTC_3129|Compound:9127257|FD-Score:4.27|P-value:7.50E-4 ID:SGTC_3140|Compound:9094243|FD-Score:-4.39|P-value:4.09E-4 ID:SGTC_3141|Compound:9094609|FD-Score:5.11|P-value:6.89E-5 ID:SGTC_3142|Compound:9086498|FD-Score:8.70|P-value:3.64E-11 ID:SGTC_3145|Compound:9085516|FD-Score:-5.51|P-value:1.37E-5 ID:SGTC_3155|Compound:9099265|FD-Score:4.81|P-value:1.67E-4 ID:SGTC_3174|Compound:9107204|FD-Score:4.90|P-value:1.32E-4 ID:SGTC_3202|Compound:9113761|FD-Score:4.45|P-value:4.56E-4 ID:SGTC_3207|Compound:9114163|FD-Score:-4.55|P-value:2.57E-4 ID:SGTC_3249|Compound:9135183|FD-Score:-4.07|P-value:9.52E-4 ID:SGTC_3266|Compound:9136860|FD-Score:-4.69|P-value:1.75E-4

Top fitness defect scores for YDL185W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3142 9086498 8.70 49.5 µM 3.64E-11 iron homeostasis 1 1.69E-18
2 SGTC_2752 diclazuril 8.16 33.1 µM 4.94E-10 32 1.64E-16
3 SGTC_498 2,5-di-tert-butyl-1,4-hydroquinone (DTBHQ) 8.04 150.0 µM 8.64E-10 Golgi 8 4.38E-16
4 SGTC_164 4100-3994 7.63 89.6 µM 5.59E-9 2 1.15E-14
5 SGTC_1600 coumarin 106 6.75 71.1 µM 2.21E-7 5 7.21E-12
6 SGTC_1658 st012944 6.55 20.0 µM 4.88E-7 iron homeostasis 23 2.86E-11
7 SGTC_1148 3909-7663 6.33 27.7 µM 1.11E-6 1 1.21E-10
8 SGTC_2841 9004761 6.06 39.0 µM 2.95E-6 amide catabolism 6 6.64E-10
9 SGTC_391 gliotoxin 5.78 8.3 µM 7.99E-6 23 3.77E-9
10 SGTC_1869 3',7'-dimethoxyflavone 5.73 40.3 µM 9.47E-6 4 5.06E-9
11 SGTC_1157 4092-0394 5.68 27.6 µM 1.12E-5 6 6.78E-9
12 SGTC_2740 felodipine 5.47 17.1 µM 2.20E-5 8 2.20E-8
13 SGTC_717 1532-0246 5.46 73.2 µM 2.31E-5 Golgi 6 2.38E-8
14 SGTC_2307 7538926 5.28 6.3 µM 4.07E-5 13 6.39E-8
15 SGTC_2339 9000433 5.22 200.0 µM 4.89E-5 10 8.81E-8
16 SGTC_939 1165-0479 5.14 45.3 µM 6.41E-5 8 1.41E-7
17 SGTC_841 0251-0215 5.12 91.2 µM 6.70E-5 52 1.52E-7
18 SGTC_3141 9094609 5.11 49.5 µM 6.89E-5 iron homeostasis 18 1.60E-7
19 SGTC_75 0159-0059 5.08 199.0 µM 7.50E-5 fatty acid desaturase (OLE1) 11 1.85E-7
20 SGTC_1239 0337-0584 5.08 35.4 µM 7.67E-5 unfolded protein response 24 1.92E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.189 3.12E-28 YPR036W VMA13 hom Subunit H of the eight-subunit V1 peripheral membrane domain of the vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; serves as an activator or a structural stabilizer of the V-ATPase
0.167 1.85E-22 YJR033C RAV1 hom Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate
0.164 1.51E-21 YGR020C VMA7 hom Subunit F of the eight-subunit V1 peripheral membrane domain of vacuolar H+-ATPase (V-ATPase), an electrogenic proton pump found throughout the endomembrane system; required for the V1 domain to assemble onto the vacuolar membrane
0.163 1.65E-21 YOR331C_d YOR331C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame overlaps the verified gene VMA4/YOR332W
0.153 4.78E-19 YJR040W GEF1 hom Voltage-gated chloride channel localized to the golgi, the endosomal system, and plasma membrane, and involved in cation homeostasis; highly homologous to vertebrate voltage-gated chloride channels; modulates TBSV model (+) RNA virus replication by regulating copper metabolism
0.147 9.28E-18 YHR026W VMA16 hom Subunit c'' of the vacuolar ATPase, which functions in acidification of the vacuole; one of three proteolipid subunits of the V0 domain
0.139 6.82E-16 YMR123W PKR1 hom V-ATPase assembly factor; functions with other V-ATPase assembly factors in the ER to efficiently assemble the V-ATPase membrane sector (V0); protein abundance increases in response to DNA replication stress
0.137 2.05E-15 YDR203W_d YDR203W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.131 2.90E-14 YGL071W AFT1 hom Transcription factor involved in iron utilization and homeostasis; binds consensus site PyPuCACCCPu and activates transcription in response to changes in iron availability; in iron-replete conditions activity is negatively regulated by Grx3p, Grx4p, and Fra2p, which regulate Aft1p translocation from the nucleus to the cytoplasm; AFT1 has a paralog, AFT2, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.124 6.28E-13 YDR270W CCC2 hom Cu(+2)-transporting P-type ATPase; required for export of copper from the cytosol into an extracytosolic compartment; has similarity to human proteins involved in Menkes and Wilsons diseases; protein abundance increases in response to DNA replication stress; affects TBSV model (+)RNA virus replication by regulating copper metabolism
0.120 3.27E-12 YKL118W_d YKL118W_d hom Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene VPH2
0.116 1.45E-11 YDR080W VPS41 hom Vacuolar membrane protein that is a subunit of the homotypic vacuole fusion and vacuole protein sorting (HOPS) complex; essential for membrane docking and fusion at the Golgi-to-endosome and endosome-to-vacuole stages of protein transport
0.114 3.88E-11 YLR372W SUR4 hom Elongase, involved in fatty acid and sphingolipid biosynthesis; synthesizes very long chain 20-26-carbon fatty acids from C18-CoA primers; involved in regulation of sphingolipid biosynthesis
0.111 1.34E-10 YMR202W ERG2 hom C-8 sterol isomerase, catalyzes the isomerization of the delta-8 double bond to the delta-7 position at an intermediate step in ergosterol biosynthesis
0.109 2.10E-10 YER145C FTR1 hom High affinity iron permease; involved in the transport of iron across the plasma membrane; forms complex with Fet3p; expression is regulated by iron; protein abundance increases in response to DNA replication stress