YDL190C / UFD2

Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL190C / UFD2

Click on Significant Values for Screen Details ID:SGTC_16|Compound:erodoxin|FD-Score:4.15|P-value:2.96E-5 ID:SGTC_36|Compound:0335-0881|FD-Score:12.00|P-value:2.21E-31 ID:SGTC_43|Compound:0570-0105|FD-Score:10.90|P-value:2.45E-26 ID:SGTC_46|Compound:0329-0187a|FD-Score:8.33|P-value:3.60E-16 ID:SGTC_55|Compound:0767-0285|FD-Score:4.67|P-value:3.07E-6 ID:SGTC_59|Compound:0330-0160|FD-Score:3.54|P-value:3.10E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:4.20|P-value:2.40E-5 ID:SGTC_103|Compound:0844-0104|FD-Score:10.00|P-value:1.35E-22 ID:SGTC_137|Compound:0438-0332|FD-Score:3.92|P-value:7.36E-5 ID:SGTC_158|Compound:k038-0087|FD-Score:3.52|P-value:3.32E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:3.39|P-value:5.18E-4 ID:SGTC_188|Compound:3229-0966|FD-Score:3.72|P-value:1.63E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-5.56|P-value:3.63E-8 ID:SGTC_312|Compound:4092-0653|FD-Score:4.45|P-value:8.20E-6 ID:SGTC_469|Compound:w7|FD-Score:3.32|P-value:6.67E-4 ID:SGTC_470|Compound:aa-861|FD-Score:3.82|P-value:1.10E-4 ID:SGTC_557|Compound:1315-0110|FD-Score:-3.66|P-value:1.96E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:4.51|P-value:6.53E-6 ID:SGTC_797|Compound:k781-0471|FD-Score:-3.48|P-value:3.67E-4 ID:SGTC_878|Compound:0828-0289|FD-Score:-3.51|P-value:3.30E-4 ID:SGTC_914|Compound:3820-6779|FD-Score:-3.38|P-value:5.30E-4 ID:SGTC_919|Compound:tnp00227|FD-Score:-3.54|P-value:2.98E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:3.35|P-value:6.02E-4 ID:SGTC_1148|Compound:3909-7663|FD-Score:-3.26|P-value:7.86E-4 ID:SGTC_1152|Compound:3909-8331|FD-Score:-3.40|P-value:4.83E-4 ID:SGTC_1173|Compound:0443-0020|FD-Score:3.67|P-value:1.93E-4 ID:SGTC_1242|Compound:0402-0103|FD-Score:-3.63|P-value:2.18E-4 ID:SGTC_1332|Compound:1480-0653|FD-Score:3.64|P-value:2.17E-4 ID:SGTC_1336|Compound:1486-0901|FD-Score:-3.70|P-value:1.66E-4 ID:SGTC_1344|Compound:1488-0097|FD-Score:3.47|P-value:3.98E-4 ID:SGTC_1356|Compound:1502-0781|FD-Score:3.34|P-value:6.27E-4 ID:SGTC_1715|Compound:st032160|FD-Score:3.99|P-value:5.57E-5 ID:SGTC_1772|Compound:st048439|FD-Score:3.93|P-value:7.11E-5 ID:SGTC_1808|Compound:st050063|FD-Score:3.44|P-value:4.38E-4 ID:SGTC_2425|Compound:5628681|FD-Score:3.31|P-value:6.93E-4 ID:SGTC_2429|Compound:5902844|FD-Score:3.53|P-value:3.23E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:4.72|P-value:2.43E-6 ID:SGTC_2691|Compound:st077356|FD-Score:-4.45|P-value:8.16E-6 ID:SGTC_2831|Compound:7999036|FD-Score:3.52|P-value:3.34E-4 ID:SGTC_2853|Compound:9015042|FD-Score:-3.86|P-value:9.09E-5 ID:SGTC_2854|Compound:9024559|FD-Score:3.75|P-value:1.45E-4 ID:SGTC_2858|Compound:9025781|FD-Score:9.62|P-value:6.16E-21 ID:SGTC_2862|Compound:9027634|FD-Score:-3.74|P-value:1.42E-4 ID:SGTC_2865|Compound:9030313|FD-Score:3.28|P-value:7.50E-4 ID:SGTC_2876|Compound:9059903|FD-Score:3.65|P-value:2.08E-4 ID:SGTC_2877|Compound:9040724|FD-Score:3.26|P-value:8.03E-4 ID:SGTC_2888|Compound:9058547|FD-Score:-3.47|P-value:3.87E-4 ID:SGTC_2896|Compound:9052656|FD-Score:3.69|P-value:1.76E-4 ID:SGTC_2951|Compound:9072950|FD-Score:-3.19|P-value:9.85E-4 ID:SGTC_2954|Compound:9074818|FD-Score:-3.78|P-value:1.22E-4 ID:SGTC_2976|Compound:9009005|FD-Score:3.31|P-value:6.74E-4 ID:SGTC_3214|Compound:9129694|FD-Score:5.02|P-value:5.94E-7 ID:SGTC_3215|Compound:9129942|FD-Score:3.53|P-value:3.16E-4 ID:SGTC_3264|Compound:9137442|FD-Score:4.49|P-value:7.03E-6 ID:SGTC_3293|Compound:9120939|FD-Score:-4.08|P-value:3.83E-5 ID:SGTC_3322|Compound:9139058|FD-Score:3.37|P-value:5.50E-4 ID:SGTC_16|Compound:erodoxin|FD-Score:4.15|P-value:2.96E-5 ID:SGTC_36|Compound:0335-0881|FD-Score:12.00|P-value:2.21E-31 ID:SGTC_43|Compound:0570-0105|FD-Score:10.90|P-value:2.45E-26 ID:SGTC_46|Compound:0329-0187a|FD-Score:8.33|P-value:3.60E-16 ID:SGTC_55|Compound:0767-0285|FD-Score:4.67|P-value:3.07E-6 ID:SGTC_59|Compound:0330-0160|FD-Score:3.54|P-value:3.10E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:4.20|P-value:2.40E-5 ID:SGTC_103|Compound:0844-0104|FD-Score:10.00|P-value:1.35E-22 ID:SGTC_137|Compound:0438-0332|FD-Score:3.92|P-value:7.36E-5 ID:SGTC_158|Compound:k038-0087|FD-Score:3.52|P-value:3.32E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:3.39|P-value:5.18E-4 ID:SGTC_188|Compound:3229-0966|FD-Score:3.72|P-value:1.63E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-5.56|P-value:3.63E-8 ID:SGTC_312|Compound:4092-0653|FD-Score:4.45|P-value:8.20E-6 ID:SGTC_469|Compound:w7|FD-Score:3.32|P-value:6.67E-4 ID:SGTC_470|Compound:aa-861|FD-Score:3.82|P-value:1.10E-4 ID:SGTC_557|Compound:1315-0110|FD-Score:-3.66|P-value:1.96E-4 ID:SGTC_669|Compound:0108-0021|FD-Score:4.51|P-value:6.53E-6 ID:SGTC_797|Compound:k781-0471|FD-Score:-3.48|P-value:3.67E-4 ID:SGTC_878|Compound:0828-0289|FD-Score:-3.51|P-value:3.30E-4 ID:SGTC_914|Compound:3820-6779|FD-Score:-3.38|P-value:5.30E-4 ID:SGTC_919|Compound:tnp00227|FD-Score:-3.54|P-value:2.98E-4 ID:SGTC_996|Compound:3970-0799|FD-Score:3.35|P-value:6.02E-4 ID:SGTC_1148|Compound:3909-7663|FD-Score:-3.26|P-value:7.86E-4 ID:SGTC_1152|Compound:3909-8331|FD-Score:-3.40|P-value:4.83E-4 ID:SGTC_1173|Compound:0443-0020|FD-Score:3.67|P-value:1.93E-4 ID:SGTC_1242|Compound:0402-0103|FD-Score:-3.63|P-value:2.18E-4 ID:SGTC_1332|Compound:1480-0653|FD-Score:3.64|P-value:2.17E-4 ID:SGTC_1336|Compound:1486-0901|FD-Score:-3.70|P-value:1.66E-4 ID:SGTC_1344|Compound:1488-0097|FD-Score:3.47|P-value:3.98E-4 ID:SGTC_1356|Compound:1502-0781|FD-Score:3.34|P-value:6.27E-4 ID:SGTC_1715|Compound:st032160|FD-Score:3.99|P-value:5.57E-5 ID:SGTC_1772|Compound:st048439|FD-Score:3.93|P-value:7.11E-5 ID:SGTC_1808|Compound:st050063|FD-Score:3.44|P-value:4.38E-4 ID:SGTC_2425|Compound:5628681|FD-Score:3.31|P-value:6.93E-4 ID:SGTC_2429|Compound:5902844|FD-Score:3.53|P-value:3.23E-4 ID:SGTC_2631|Compound:bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone|FD-Score:4.72|P-value:2.43E-6 ID:SGTC_2691|Compound:st077356|FD-Score:-4.45|P-value:8.16E-6 ID:SGTC_2831|Compound:7999036|FD-Score:3.52|P-value:3.34E-4 ID:SGTC_2853|Compound:9015042|FD-Score:-3.86|P-value:9.09E-5 ID:SGTC_2854|Compound:9024559|FD-Score:3.75|P-value:1.45E-4 ID:SGTC_2858|Compound:9025781|FD-Score:9.62|P-value:6.16E-21 ID:SGTC_2862|Compound:9027634|FD-Score:-3.74|P-value:1.42E-4 ID:SGTC_2865|Compound:9030313|FD-Score:3.28|P-value:7.50E-4 ID:SGTC_2876|Compound:9059903|FD-Score:3.65|P-value:2.08E-4 ID:SGTC_2877|Compound:9040724|FD-Score:3.26|P-value:8.03E-4 ID:SGTC_2888|Compound:9058547|FD-Score:-3.47|P-value:3.87E-4 ID:SGTC_2896|Compound:9052656|FD-Score:3.69|P-value:1.76E-4 ID:SGTC_2951|Compound:9072950|FD-Score:-3.19|P-value:9.85E-4 ID:SGTC_2954|Compound:9074818|FD-Score:-3.78|P-value:1.22E-4 ID:SGTC_2976|Compound:9009005|FD-Score:3.31|P-value:6.74E-4 ID:SGTC_3214|Compound:9129694|FD-Score:5.02|P-value:5.94E-7 ID:SGTC_3215|Compound:9129942|FD-Score:3.53|P-value:3.16E-4 ID:SGTC_3264|Compound:9137442|FD-Score:4.49|P-value:7.03E-6 ID:SGTC_3293|Compound:9120939|FD-Score:-4.08|P-value:3.83E-5 ID:SGTC_3322|Compound:9139058|FD-Score:3.37|P-value:5.50E-4

Top fitness defect scores for YDL190C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_36 0335-0881 12.00 14.2 µM 2.21E-31 ubiquinone biosynthesis & proteasome 15 2.37E-33
2 SGTC_43 0570-0105 10.90 72.5 µM 2.45E-26 ERG2 8 5.67E-28
3 SGTC_103 0844-0104 10.00 51.8 µM 1.35E-22 ERAD & cell cycle 30 5.51E-24
4 SGTC_2858 9025781 9.62 9.0 µM 6.16E-21 sphingolipid biosynthesis & PDR1 23 3.24E-22
5 SGTC_46 0329-0187a 8.33 2.2 µM 3.60E-16 14 3.91E-17
6 SGTC_3214 9129694 5.02 49.5 µM 5.94E-7 9 2.58E-7
7 SGTC_2631 bromo-3-hydroxy-4-(succin-2-yl)-caryolane gamma-lactone 4.72 100.0 µM 2.43E-6 20 1.16E-6
8 SGTC_55 0767-0285 4.67 6.6 µM 3.07E-6 33 1.49E-6
9 SGTC_669 0108-0021 4.51 26.2 µM 6.53E-6 fatty acid desaturase (OLE1) 58 3.31E-6
10 SGTC_3264 9137442 4.49 49.5 µM 7.03E-6 9 3.58E-6
11 SGTC_312 4092-0653 4.45 6.8 µM 8.20E-6 ergosterol depletion effects on membrane 71 4.23E-6
12 SGTC_67 0335-0847 4.20 266.0 µM 2.40E-5 ubiquinone biosynthesis & proteasome 102 1.33E-5
13 SGTC_16 erodoxin 4.15 22.8 µM 2.96E-5 unfolded protein response 60 1.66E-5
14 SGTC_1715 st032160 3.99 10.2 µM 5.57E-5 42 3.25E-5
15 SGTC_1772 st048439 3.93 82.2 µM 7.11E-5 38 4.21E-5
16 SGTC_137 0438-0332 3.92 85.4 µM 7.36E-5 ubiquinone biosynthesis & proteasome 124 4.36E-5
17 SGTC_470 aa-861 3.82 153.0 µM 1.10E-4 sphingolipid biosynthesis & PDR1 60 6.68E-5
18 SGTC_2854 9024559 3.75 71.4 µM 1.45E-4 29 8.99E-5
19 SGTC_188 3229-0966 3.72 278.5 µM 1.63E-4 31 1.02E-4
20 SGTC_2896 9052656 3.69 71.4 µM 1.76E-4 endomembrane recycling 139 1.10E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.248 2.93E-48 YER041W YEN1 hom Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p
0.243 1.95E-46 YPL153C RAD53 het Protein kinase, required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication
0.226 4.08E-40 YDR537C_d YDR537C_d hom Dubious open reading frame unlikely to encode a protein, almost completely overlaps verified ORF PAD1/YDR538W
0.220 4.22E-38 YNR042W_d YNR042W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps verified gene COQ2
0.218 2.06E-37 YER019C-A SBH2 hom Ssh1p-Sss1p-Sbh2p complex component, involved in protein translocation into the endoplasmic reticulum; homologous to Sbh1p
0.213 9.30E-36 YMR067C UBX4 hom UBX domain-containing protein that interacts with Cdc48p; involved in degradation of polyubiquitinated proteins via the ERAD (ER-associated degradation) pathway; modulates the Cdc48p-Nplp-Ufd1p AAA ATPase complex during its role in delivery of misfolded proteins to the proteasome; protein abundance increases in response to DNA replication stress
0.210 1.24E-34 YJL072C PSF2 het Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
0.206 2.10E-33 YPL124W SPC29 het Inner plaque spindle pole body (SPB) component, links the central plaque component Spc42p to the inner plaque component Spc110p; required for SPB duplication
0.203 1.58E-32 YDL122W UBP1 hom Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains
0.200 1.56E-31 YGR035C_p YGR035C_p hom Putative protein of unknown function, potential Cdc28p substrate; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance
0.198 7.70E-31 YDL183C YDL183C hom Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene
0.192 2.54E-29 YML034C-A_d YML034C-A_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.192 4.42E-29 YIL090W ICE2 hom Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells
0.191 5.97E-29 YLR201C COQ9 hom Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes
0.182 1.75E-26 YPR064W_d YPR064W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data