YDL203C / ACK1

Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL203C / ACK1

Click on Significant Values for Screen Details ID:SGTC_16|Compound:erodoxin|FD-Score:6.30|P-value:2.27E-10 ID:SGTC_22|Compound:0964-0034|FD-Score:3.46|P-value:3.03E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:20.30|P-value:8.36E-90 ID:SGTC_65|Compound:0798-0905|FD-Score:3.79|P-value:8.61E-5 ID:SGTC_138|Compound:0850-0259|FD-Score:25.90|P-value:1.51E-144 ID:SGTC_255|Compound:tunicamycin|FD-Score:12.90|P-value:2.55E-37 ID:SGTC_304|Compound:1310-0298|FD-Score:7.23|P-value:4.02E-13 ID:SGTC_351|Compound:caspofungin|FD-Score:4.52|P-value:3.67E-6 ID:SGTC_354|Compound:2783-3500|FD-Score:6.32|P-value:1.94E-10 ID:SGTC_360|Compound:0957-0399|FD-Score:12.80|P-value:8.03E-37 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:4.52|P-value:3.69E-6 ID:SGTC_500|Compound:h-89|FD-Score:4.34|P-value:8.31E-6 ID:SGTC_514|Compound:4466-0038|FD-Score:4.93|P-value:5.27E-7 ID:SGTC_586|Compound:0957-0265|FD-Score:6.07|P-value:9.53E-10 ID:SGTC_663|Compound:1486-0733|FD-Score:23.00|P-value:1.20E-114 ID:SGTC_666|Compound:0272-0154|FD-Score:7.90|P-value:2.80E-15 ID:SGTC_863|Compound:0466-0191|FD-Score:18.00|P-value:8.59E-71 ID:SGTC_916|Compound:4466-0038|FD-Score:10.60|P-value:4.59E-26 ID:SGTC_928|Compound:2764-0428|FD-Score:3.62|P-value:1.68E-4 ID:SGTC_973|Compound:1325-0077|FD-Score:3.40|P-value:3.66E-4 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.25|P-value:6.31E-4 ID:SGTC_1205|Compound:4161-2785|FD-Score:-3.40|P-value:4.18E-4 ID:SGTC_1214|Compound:0358-0031|FD-Score:7.16|P-value:7.08E-13 ID:SGTC_1343|Compound:1487-0095|FD-Score:3.22|P-value:6.89E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:4.06|P-value:2.86E-5 ID:SGTC_1376|Compound:2910-0861|FD-Score:-4.33|P-value:1.04E-5 ID:SGTC_1739|Compound:st037196|FD-Score:4.00|P-value:3.70E-5 ID:SGTC_2001|Compound:4023502|FD-Score:-3.26|P-value:6.80E-4 ID:SGTC_2005|Compound:4024620|FD-Score:-3.28|P-value:6.31E-4 ID:SGTC_2007|Compound:4011539|FD-Score:21.00|P-value:1.07E-95 ID:SGTC_2009|Compound:4012297|FD-Score:18.20|P-value:2.54E-72 ID:SGTC_2272|Compound:7805186|FD-Score:3.28|P-value:5.63E-4 ID:SGTC_2331|Compound:7980721|FD-Score:3.97|P-value:4.16E-5 ID:SGTC_2335|Compound:9018011|FD-Score:3.21|P-value:7.31E-4 ID:SGTC_2399|Compound:5884133|FD-Score:9.49|P-value:3.07E-21 ID:SGTC_2582|Compound:agaric acid|FD-Score:5.50|P-value:2.56E-8 ID:SGTC_2584|Compound:androsterone|FD-Score:19.70|P-value:1.18E-84 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:3.35|P-value:4.50E-4 ID:SGTC_2602|Compound:kawain|FD-Score:3.39|P-value:3.78E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:4.28|P-value:1.09E-5 ID:SGTC_2687|Compound:chenodiol|FD-Score:-4.21|P-value:1.72E-5 ID:SGTC_2695|Compound:st077686|FD-Score:-5.22|P-value:1.34E-7 ID:SGTC_2912|Compound:7964298|FD-Score:7.99|P-value:1.32E-15 ID:SGTC_2979|Compound:9012552|FD-Score:-4.08|P-value:3.03E-5 ID:SGTC_2987|Compound:9018338|FD-Score:-3.72|P-value:1.27E-4 ID:SGTC_3311|Compound:9131146|FD-Score:14.70|P-value:5.17E-48 ID:SGTC_3321|Compound:9137793|FD-Score:16.60|P-value:1.32E-60 ID:SGTC_3322|Compound:9139058|FD-Score:5.31|P-value:7.05E-8 ID:SGTC_3350|Compound:9150499|FD-Score:5.45|P-value:3.30E-8 ID:SGTC_16|Compound:erodoxin|FD-Score:6.30|P-value:2.27E-10 ID:SGTC_22|Compound:0964-0034|FD-Score:3.46|P-value:3.03E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:20.30|P-value:8.36E-90 ID:SGTC_65|Compound:0798-0905|FD-Score:3.79|P-value:8.61E-5 ID:SGTC_138|Compound:0850-0259|FD-Score:25.90|P-value:1.51E-144 ID:SGTC_255|Compound:tunicamycin|FD-Score:12.90|P-value:2.55E-37 ID:SGTC_304|Compound:1310-0298|FD-Score:7.23|P-value:4.02E-13 ID:SGTC_351|Compound:caspofungin|FD-Score:4.52|P-value:3.67E-6 ID:SGTC_354|Compound:2783-3500|FD-Score:6.32|P-value:1.94E-10 ID:SGTC_360|Compound:0957-0399|FD-Score:12.80|P-value:8.03E-37 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:4.52|P-value:3.69E-6 ID:SGTC_500|Compound:h-89|FD-Score:4.34|P-value:8.31E-6 ID:SGTC_514|Compound:4466-0038|FD-Score:4.93|P-value:5.27E-7 ID:SGTC_586|Compound:0957-0265|FD-Score:6.07|P-value:9.53E-10 ID:SGTC_663|Compound:1486-0733|FD-Score:23.00|P-value:1.20E-114 ID:SGTC_666|Compound:0272-0154|FD-Score:7.90|P-value:2.80E-15 ID:SGTC_863|Compound:0466-0191|FD-Score:18.00|P-value:8.59E-71 ID:SGTC_916|Compound:4466-0038|FD-Score:10.60|P-value:4.59E-26 ID:SGTC_928|Compound:2764-0428|FD-Score:3.62|P-value:1.68E-4 ID:SGTC_973|Compound:1325-0077|FD-Score:3.40|P-value:3.66E-4 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.25|P-value:6.31E-4 ID:SGTC_1205|Compound:4161-2785|FD-Score:-3.40|P-value:4.18E-4 ID:SGTC_1214|Compound:0358-0031|FD-Score:7.16|P-value:7.08E-13 ID:SGTC_1343|Compound:1487-0095|FD-Score:3.22|P-value:6.89E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:4.06|P-value:2.86E-5 ID:SGTC_1376|Compound:2910-0861|FD-Score:-4.33|P-value:1.04E-5 ID:SGTC_1739|Compound:st037196|FD-Score:4.00|P-value:3.70E-5 ID:SGTC_2001|Compound:4023502|FD-Score:-3.26|P-value:6.80E-4 ID:SGTC_2005|Compound:4024620|FD-Score:-3.28|P-value:6.31E-4 ID:SGTC_2007|Compound:4011539|FD-Score:21.00|P-value:1.07E-95 ID:SGTC_2009|Compound:4012297|FD-Score:18.20|P-value:2.54E-72 ID:SGTC_2272|Compound:7805186|FD-Score:3.28|P-value:5.63E-4 ID:SGTC_2331|Compound:7980721|FD-Score:3.97|P-value:4.16E-5 ID:SGTC_2335|Compound:9018011|FD-Score:3.21|P-value:7.31E-4 ID:SGTC_2399|Compound:5884133|FD-Score:9.49|P-value:3.07E-21 ID:SGTC_2582|Compound:agaric acid|FD-Score:5.50|P-value:2.56E-8 ID:SGTC_2584|Compound:androsterone|FD-Score:19.70|P-value:1.18E-84 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:3.35|P-value:4.50E-4 ID:SGTC_2602|Compound:kawain|FD-Score:3.39|P-value:3.78E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:4.28|P-value:1.09E-5 ID:SGTC_2687|Compound:chenodiol|FD-Score:-4.21|P-value:1.72E-5 ID:SGTC_2695|Compound:st077686|FD-Score:-5.22|P-value:1.34E-7 ID:SGTC_2912|Compound:7964298|FD-Score:7.99|P-value:1.32E-15 ID:SGTC_2979|Compound:9012552|FD-Score:-4.08|P-value:3.03E-5 ID:SGTC_2987|Compound:9018338|FD-Score:-3.72|P-value:1.27E-4 ID:SGTC_3311|Compound:9131146|FD-Score:14.70|P-value:5.17E-48 ID:SGTC_3321|Compound:9137793|FD-Score:16.60|P-value:1.32E-60 ID:SGTC_3322|Compound:9139058|FD-Score:5.31|P-value:7.05E-8 ID:SGTC_3350|Compound:9150499|FD-Score:5.45|P-value:3.30E-8

Top fitness defect scores for YDL203C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_138 0850-0259 25.90 40.9 µM 1.51E-144 cell wall 2 5.77E-148
2 SGTC_663 1486-0733 23.00 13.9 µM 1.20E-114 cell wall signaling 3 2.50E-117
3 SGTC_2007 4011539 21.00 71.8 µM 1.07E-95 cell wall signaling 2 6.47E-98
4 SGTC_58 0957-0393 20.30 9.0 µM 8.36E-90 cell wall 8 7.04E-92
5 SGTC_2584 androsterone 19.70 48.7 µM 1.18E-84 cell wall 1 1.32E-86
6 SGTC_2009 4012297 18.20 12.1 µM 2.54E-72 cell wall signaling 4 5.70E-74
7 SGTC_863 0466-0191 18.00 129.0 µM 8.59E-71 cell wall 3 2.10E-72
8 SGTC_3321 9137793 16.60 26.8 µM 1.32E-60 cell wall 1 5.72E-62
9 SGTC_3311 9131146 14.70 2.9 µM 5.17E-48 cell wall 7 4.50E-49
10 SGTC_255 tunicamycin 12.90 200.5 nM 2.55E-37 unfolded protein response 5 4.01E-38
11 SGTC_360 0957-0399 12.80 1.0 µM 8.03E-37 cell wall 5 1.30E-37
12 SGTC_916 4466-0038 10.60 42.0 nM 4.59E-26 cell wall 6 1.33E-26
13 SGTC_2399 5884133 9.49 200.0 µM 3.07E-21 cell wall signaling 14 1.16E-21
14 SGTC_2912 7964298 7.99 10.9 µM 1.32E-15 cell wall 26 6.75E-16
15 SGTC_666 0272-0154 7.90 136.0 µM 2.80E-15 cell wall 9 1.45E-15
16 SGTC_304 1310-0298 7.23 97.0 µM 4.02E-13 cell wall 9 2.33E-13
17 SGTC_1214 0358-0031 7.16 63.7 µM 7.08E-13 2 4.17E-13
18 SGTC_354 2783-3500 6.32 4.9 µM 1.94E-10 cell wall 17 1.29E-10
19 SGTC_16 erodoxin 6.30 22.8 µM 2.27E-10 unfolded protein response 24 1.52E-10
20 SGTC_586 0957-0265 6.07 8.2 µM 9.53E-10 cell wall 17 6.59E-10

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.724 0 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.635 0 YLR332W MID2 hom O-glycosylated plasma membrane protein that acts as a sensor for cell wall integrity signaling and activates the pathway; interacts with Rom2p, a guanine nucleotide exchange factor for Rho1p, and with cell integrity pathway protein Zeo1p
0.569 4.78E-287 YJL099W CHS6 hom Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.564 2.05E-281 YBR260C RGD1 hom GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization
0.527 3.14E-239 YGR196C FYV8 hom Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.488 1.30E-200 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.469 4.38E-183 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.461 1.39E-176 YPL115C BEM3 hom Rho GTPase activating protein (RhoGAP) involved in control of the cytoskeleton organization; targets the essential Rho-GTPase Cdc42p, which controls establishment and maintenance of cell polarity, including bud-site assembly
0.454 3.04E-170 YMR124W_p YMR124W_p hom Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.444 2.75E-162 YKR042W UTH1 hom Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication
0.439 2.15E-158 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.437 8.26E-157 YPR165W RHO1 het GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
0.432 2.53E-152 YBL006C LDB7 hom Component of the RSC chromatin remodeling complex; interacts with Rsc3p, Rsc30p, Npl6p, and Htl1p to form a module important for a broad range of RSC functions
0.426 7.69E-148 YBR023C CHS3 hom Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan
0.422 2.28E-145 YLL043W FPS1 hom Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress