YDL215C / GDH2

NAD(+)-dependent glutamate dehydrogenase, degrades glutamate to ammonia and alpha-ketoglutarate; expression sensitive to nitrogen catabolite repression and intracellular ammonia levels

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL215C / GDH2

Click on Significant Values for Screen Details ID:SGTC_45|Compound:0kpi-0036|FD-Score:-3.00|P-value:5.58E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:2.92|P-value:8.15E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:4.17|P-value:3.25E-6 ID:SGTC_75|Compound:0159-0059|FD-Score:5.35|P-value:3.63E-9 ID:SGTC_346|Compound:4513-0042|FD-Score:-2.94|P-value:6.79E-4 ID:SGTC_355|Compound:1311-2252|FD-Score:-3.05|P-value:4.51E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:3.17|P-value:3.11E-4 ID:SGTC_384|Compound:k007-0144|FD-Score:2.87|P-value:9.79E-4 ID:SGTC_537|Compound:1334-0398|FD-Score:3.86|P-value:1.51E-5 ID:SGTC_671|Compound:0560-0003|FD-Score:3.26|P-value:2.18E-4 ID:SGTC_763|Compound:3453-1109|FD-Score:3.67|P-value:3.65E-5 ID:SGTC_952|Compound:1185-0122|FD-Score:-3.45|P-value:8.62E-5 ID:SGTC_1042|Compound:0350-0096|FD-Score:-3.23|P-value:2.21E-4 ID:SGTC_1583|Compound:clindamycin|FD-Score:3.38|P-value:1.30E-4 ID:SGTC_1733|Compound:st037357|FD-Score:-2.93|P-value:7.04E-4 ID:SGTC_1984|Compound:st072354|FD-Score:-2.98|P-value:5.95E-4 ID:SGTC_1986|Compound:st071812|FD-Score:-2.98|P-value:6.03E-4 ID:SGTC_2005|Compound:4024620|FD-Score:-3.13|P-value:3.26E-4 ID:SGTC_2007|Compound:4011539|FD-Score:-2.92|P-value:7.45E-4 ID:SGTC_2016|Compound:5121758|FD-Score:-2.84|P-value:9.85E-4 ID:SGTC_2344|Compound:7990960|FD-Score:-2.92|P-value:7.35E-4 ID:SGTC_2350|Compound:9023895|FD-Score:-3.13|P-value:3.32E-4 ID:SGTC_2460|Compound:5552655|FD-Score:3.28|P-value:1.93E-4 ID:SGTC_2606|Compound:farnesol|FD-Score:3.12|P-value:3.74E-4 ID:SGTC_2786|Compound:7743617|FD-Score:-3.57|P-value:5.25E-5 ID:SGTC_2839|Compound:9003951|FD-Score:3.27|P-value:2.07E-4 ID:SGTC_2840|Compound:9008771|FD-Score:3.31|P-value:1.77E-4 ID:SGTC_2939|Compound:9044578|FD-Score:-2.89|P-value:8.32E-4 ID:SGTC_2940|Compound:9060638|FD-Score:-3.37|P-value:1.23E-4 ID:SGTC_2989|Compound:9073649|FD-Score:3.35|P-value:1.47E-4 ID:SGTC_3081|Compound:9117243|FD-Score:3.55|P-value:6.20E-5 ID:SGTC_3200|Compound:9114385|FD-Score:4.24|P-value:2.32E-6 ID:SGTC_45|Compound:0kpi-0036|FD-Score:-3.00|P-value:5.58E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:2.92|P-value:8.15E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:4.17|P-value:3.25E-6 ID:SGTC_75|Compound:0159-0059|FD-Score:5.35|P-value:3.63E-9 ID:SGTC_346|Compound:4513-0042|FD-Score:-2.94|P-value:6.79E-4 ID:SGTC_355|Compound:1311-2252|FD-Score:-3.05|P-value:4.51E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:3.17|P-value:3.11E-4 ID:SGTC_384|Compound:k007-0144|FD-Score:2.87|P-value:9.79E-4 ID:SGTC_537|Compound:1334-0398|FD-Score:3.86|P-value:1.51E-5 ID:SGTC_671|Compound:0560-0003|FD-Score:3.26|P-value:2.18E-4 ID:SGTC_763|Compound:3453-1109|FD-Score:3.67|P-value:3.65E-5 ID:SGTC_952|Compound:1185-0122|FD-Score:-3.45|P-value:8.62E-5 ID:SGTC_1042|Compound:0350-0096|FD-Score:-3.23|P-value:2.21E-4 ID:SGTC_1583|Compound:clindamycin|FD-Score:3.38|P-value:1.30E-4 ID:SGTC_1733|Compound:st037357|FD-Score:-2.93|P-value:7.04E-4 ID:SGTC_1984|Compound:st072354|FD-Score:-2.98|P-value:5.95E-4 ID:SGTC_1986|Compound:st071812|FD-Score:-2.98|P-value:6.03E-4 ID:SGTC_2005|Compound:4024620|FD-Score:-3.13|P-value:3.26E-4 ID:SGTC_2007|Compound:4011539|FD-Score:-2.92|P-value:7.45E-4 ID:SGTC_2016|Compound:5121758|FD-Score:-2.84|P-value:9.85E-4 ID:SGTC_2344|Compound:7990960|FD-Score:-2.92|P-value:7.35E-4 ID:SGTC_2350|Compound:9023895|FD-Score:-3.13|P-value:3.32E-4 ID:SGTC_2460|Compound:5552655|FD-Score:3.28|P-value:1.93E-4 ID:SGTC_2606|Compound:farnesol|FD-Score:3.12|P-value:3.74E-4 ID:SGTC_2786|Compound:7743617|FD-Score:-3.57|P-value:5.25E-5 ID:SGTC_2839|Compound:9003951|FD-Score:3.27|P-value:2.07E-4 ID:SGTC_2840|Compound:9008771|FD-Score:3.31|P-value:1.77E-4 ID:SGTC_2939|Compound:9044578|FD-Score:-2.89|P-value:8.32E-4 ID:SGTC_2940|Compound:9060638|FD-Score:-3.37|P-value:1.23E-4 ID:SGTC_2989|Compound:9073649|FD-Score:3.35|P-value:1.47E-4 ID:SGTC_3081|Compound:9117243|FD-Score:3.55|P-value:6.20E-5 ID:SGTC_3200|Compound:9114385|FD-Score:4.24|P-value:2.32E-6

Top fitness defect scores for YDL215C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_75 0159-0059 5.35 199.0 µM 3.63E-9 fatty acid desaturase (OLE1) 9 4.48E-8
2 SGTC_3200 9114385 4.24 49.5 µM 2.32E-6 24 1.14E-5
3 SGTC_70 0141-0289 4.17 43.3 µM 3.25E-6 mitochondrial stress 156 1.52E-5
4 SGTC_537 1334-0398 3.86 209.0 µM 1.51E-5 29 5.69E-5
5 SGTC_763 3453-1109 3.67 128.0 µM 3.65E-5 26 1.22E-4
6 SGTC_3081 9117243 3.55 49.5 µM 6.20E-5 fatty acid desaturase (OLE1) 30 1.92E-4
7 SGTC_1583 clindamycin 3.38 39.6 µM 1.30E-4 mitochondrial processes 33 3.63E-4
8 SGTC_2989 9073649 3.35 71.4 µM 1.47E-4 67 4.03E-4
9 SGTC_2840 9008771 3.31 71.4 µM 1.77E-4 21 4.73E-4
10 SGTC_2460 5552655 3.28 174.2 µM 1.93E-4 amide catabolism 56 5.11E-4
11 SGTC_2839 9003951 3.27 26.0 µM 2.07E-4 33 5.42E-4
12 SGTC_671 0560-0003 3.26 407.0 µM 2.18E-4 31 5.66E-4
13 SGTC_372 0155-0182 3.17 15.9 µM 3.11E-4 mitochondrial response to ROS 66 7.71E-4
14 SGTC_2606 farnesol 3.12 9.8 µM 3.74E-4 59 9.02E-4
15 SGTC_67 0335-0847 2.92 266.0 µM 8.15E-4 ubiquinone biosynthesis & proteasome 194 0.00177
16 SGTC_384 k007-0144 2.87 5.2 µM 9.79E-4 ERAD & cell cycle 128 0.00207
17 SGTC_2125 5246583 2.78 200.0 µM 0.00133 tubulin folding & SWR complex 117 0.00270
18 SGTC_2777 climbazole 2.78 1.5 µM 0.00136 iron homeostasis 118 0.00275
19 SGTC_1039 k821-0043 2.77 94.0 µM 0.00138 Golgi 88 0.00278
20 SGTC_1596 piperlongumine 2.73 63.0 µM 0.00162 69 0.00321

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.116 1.47E-11 YGR292W MAL12 hom Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL1 complex locus; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose
0.109 2.20E-10 YJR035W RAD26 hom Protein involved in transcription-coupled nucleotide excision repair of UV-induced DNA lesions; recruitment to DNA lesions is dependent on an elongating RNA polymerase II; homolog of human CSB protein
0.107 4.73E-10 YDR414C ERD1 hom Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p)
0.106 6.53E-10 YHR001W-A QCR10 hom Subunit of the ubiqunol-cytochrome c oxidoreductase complex which includes Cobp, Rip1p, Cyt1p, Cor1p, Qcr2p, Qcr6p, Qcr7p, Qcr8p, Qcr9p, and Qcr10p and comprises part of the mitochondrial respiratory chain
0.104 1.51E-9 YCL074W YCL074W hom Pseudogene: encodes fragment of Ty Pol protein
0.104 1.66E-9 YLR063W_p YLR063W_p hom Putative S-adenosylmethionine-dependent methyltransferase; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLR063W is not an essential gene
0.101 4.38E-9 YOR109W INP53 hom Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains
0.098 1.38E-8 YOR020W-A_p YOR020W-A_p hom Putative protein of unknown function, conserved in A. gossypii; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.097 1.80E-8 YAR029W_p YAR029W_p hom Member of DUP240 gene family but contains no transmembrane domains; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
0.095 3.52E-8 YDR509W_d YDR509W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.095 4.04E-8 YGR044C RME1 hom Zinc finger protein involved in control of meiosis; prevents meiosis by repressing IME1 expression and promotes mitosis by activating CLN2 expression; directly repressed by a1-alpha2 regulator; mediates cell type control of sporulation; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.094 5.61E-8 YPL036W PMA2 hom Plasma membrane H+-ATPase, isoform of Pma1p, involved in pumping protons out of the cell; regulator of cytoplasmic pH and plasma membrane potential
0.092 8.34E-8 YBR131W CCZ1 hom Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway
0.092 9.84E-8 YPL236C ENV7 hom Vacuolar membrane protein kinase; negatively regulates membrane fusion; associates with vacuolar membrane through palmitoylation of one or more cysteines in consensus sequence; vacuolar membrane association is essential to its kinase activity; mutant shows defect in CPY processing; ortholog of human serine/threonine kinase 16 (STK16)
0.091 1.49E-7 YNL257C SIP3 hom Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication