YDL216C / RRI1

Catalytic subunit of the COP9 signalosome (CSN) complex that acts as an isopeptidase in cleaving the ubiquitin-like protein Nedd8 from SCF ubiquitin ligases; metalloendopeptidase involved in the adaptation to pheromone signaling

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL216C / RRI1

Click on Significant Values for Screen Details ID:SGTC_121|Compound:0557-0495|FD-Score:4.54|P-value:9.54E-6 ID:SGTC_143|Compound:4032-1194|FD-Score:5.97|P-value:9.65E-9 ID:SGTC_146|Compound:3448-8650|FD-Score:6.28|P-value:1.71E-9 ID:SGTC_151|Compound:3013-0144|FD-Score:3.86|P-value:1.33E-4 ID:SGTC_155|Compound:k072-0202|FD-Score:5.66|P-value:4.84E-8 ID:SGTC_252|Compound:miconazole|FD-Score:-4.07|P-value:7.79E-5 ID:SGTC_333|Compound:3486-0131|FD-Score:3.64|P-value:2.99E-4 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:3.42|P-value:6.21E-4 ID:SGTC_464|Compound:bw-b 70c|FD-Score:-3.48|P-value:6.21E-4 ID:SGTC_516|Compound:3992-2719|FD-Score:4.19|P-value:3.89E-5 ID:SGTC_722|Compound:3534-0461|FD-Score:4.14|P-value:4.76E-5 ID:SGTC_751|Compound:0833-0568|FD-Score:3.55|P-value:4.00E-4 ID:SGTC_775|Compound:4577-0580|FD-Score:-3.36|P-value:9.09E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:5.24|P-value:3.96E-7 ID:SGTC_786|Compound:4544-0069|FD-Score:6.87|P-value:5.05E-11 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:4.75|P-value:3.79E-6 ID:SGTC_947|Compound:3447-0010|FD-Score:4.19|P-value:3.89E-5 ID:SGTC_948|Compound:3448-0295|FD-Score:3.79|P-value:1.74E-4 ID:SGTC_1091|Compound:telmisartan|FD-Score:3.44|P-value:5.77E-4 ID:SGTC_1130|Compound:3454-2858|FD-Score:3.52|P-value:4.44E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:5.10|P-value:7.78E-7 ID:SGTC_1204|Compound:4049-0315|FD-Score:-3.35|P-value:9.35E-4 ID:SGTC_1270|Compound:0831-1170|FD-Score:-3.55|P-value:4.84E-4 ID:SGTC_1274|Compound:0865-0114|FD-Score:3.83|P-value:1.49E-4 ID:SGTC_1292|Compound:0kpi-0043|FD-Score:3.89|P-value:1.21E-4 ID:SGTC_1487|Compound:3970-0795|FD-Score:-4.48|P-value:1.53E-5 ID:SGTC_1666|Compound:st014829|FD-Score:3.49|P-value:4.99E-4 ID:SGTC_1697|Compound:st025554|FD-Score:3.43|P-value:6.04E-4 ID:SGTC_1879|Compound:gemcitabine|FD-Score:4.02|P-value:7.45E-5 ID:SGTC_1942|Compound:st076330|FD-Score:-4.91|P-value:2.43E-6 ID:SGTC_1955|Compound:wogonin|FD-Score:4.27|P-value:2.89E-5 ID:SGTC_1978|Compound:st071761|FD-Score:3.30|P-value:9.00E-4 ID:SGTC_2026|Compound:5148700|FD-Score:8.36|P-value:1.96E-15 ID:SGTC_2028|Compound:5153890|FD-Score:4.74|P-value:3.92E-6 ID:SGTC_2039|Compound:Aminomethylbenzodioxan|FD-Score:-3.87|P-value:1.63E-4 ID:SGTC_2048|Compound:5246248|FD-Score:3.61|P-value:3.30E-4 ID:SGTC_2319|Compound:6033012|FD-Score:3.30|P-value:9.05E-4 ID:SGTC_2479|Compound:5617353|FD-Score:-4.72|P-value:5.50E-6 ID:SGTC_2482|Compound:5981709|FD-Score:5.33|P-value:2.54E-7 ID:SGTC_2611|Compound:pyromycin|FD-Score:4.23|P-value:3.28E-5 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-3.76|P-value:2.37E-4 ID:SGTC_2663|Compound:amphotericin b|FD-Score:3.39|P-value:6.74E-4 ID:SGTC_2781|Compound:5567276|FD-Score:4.46|P-value:1.32E-5 ID:SGTC_3127|Compound:9126946|FD-Score:-3.33|P-value:9.91E-4 ID:SGTC_3149|Compound:9097855|FD-Score:-3.58|P-value:4.37E-4 ID:SGTC_121|Compound:0557-0495|FD-Score:4.54|P-value:9.54E-6 ID:SGTC_143|Compound:4032-1194|FD-Score:5.97|P-value:9.65E-9 ID:SGTC_146|Compound:3448-8650|FD-Score:6.28|P-value:1.71E-9 ID:SGTC_151|Compound:3013-0144|FD-Score:3.86|P-value:1.33E-4 ID:SGTC_155|Compound:k072-0202|FD-Score:5.66|P-value:4.84E-8 ID:SGTC_252|Compound:miconazole|FD-Score:-4.07|P-value:7.79E-5 ID:SGTC_333|Compound:3486-0131|FD-Score:3.64|P-value:2.99E-4 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:3.42|P-value:6.21E-4 ID:SGTC_464|Compound:bw-b 70c|FD-Score:-3.48|P-value:6.21E-4 ID:SGTC_516|Compound:3992-2719|FD-Score:4.19|P-value:3.89E-5 ID:SGTC_722|Compound:3534-0461|FD-Score:4.14|P-value:4.76E-5 ID:SGTC_751|Compound:0833-0568|FD-Score:3.55|P-value:4.00E-4 ID:SGTC_775|Compound:4577-0580|FD-Score:-3.36|P-value:9.09E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:5.24|P-value:3.96E-7 ID:SGTC_786|Compound:4544-0069|FD-Score:6.87|P-value:5.05E-11 ID:SGTC_920|Compound:mycophenolic acid|FD-Score:4.75|P-value:3.79E-6 ID:SGTC_947|Compound:3447-0010|FD-Score:4.19|P-value:3.89E-5 ID:SGTC_948|Compound:3448-0295|FD-Score:3.79|P-value:1.74E-4 ID:SGTC_1091|Compound:telmisartan|FD-Score:3.44|P-value:5.77E-4 ID:SGTC_1130|Compound:3454-2858|FD-Score:3.52|P-value:4.44E-4 ID:SGTC_1137|Compound:ixabepilone|FD-Score:5.10|P-value:7.78E-7 ID:SGTC_1204|Compound:4049-0315|FD-Score:-3.35|P-value:9.35E-4 ID:SGTC_1270|Compound:0831-1170|FD-Score:-3.55|P-value:4.84E-4 ID:SGTC_1274|Compound:0865-0114|FD-Score:3.83|P-value:1.49E-4 ID:SGTC_1292|Compound:0kpi-0043|FD-Score:3.89|P-value:1.21E-4 ID:SGTC_1487|Compound:3970-0795|FD-Score:-4.48|P-value:1.53E-5 ID:SGTC_1666|Compound:st014829|FD-Score:3.49|P-value:4.99E-4 ID:SGTC_1697|Compound:st025554|FD-Score:3.43|P-value:6.04E-4 ID:SGTC_1879|Compound:gemcitabine|FD-Score:4.02|P-value:7.45E-5 ID:SGTC_1942|Compound:st076330|FD-Score:-4.91|P-value:2.43E-6 ID:SGTC_1955|Compound:wogonin|FD-Score:4.27|P-value:2.89E-5 ID:SGTC_1978|Compound:st071761|FD-Score:3.30|P-value:9.00E-4 ID:SGTC_2026|Compound:5148700|FD-Score:8.36|P-value:1.96E-15 ID:SGTC_2028|Compound:5153890|FD-Score:4.74|P-value:3.92E-6 ID:SGTC_2039|Compound:Aminomethylbenzodioxan|FD-Score:-3.87|P-value:1.63E-4 ID:SGTC_2048|Compound:5246248|FD-Score:3.61|P-value:3.30E-4 ID:SGTC_2319|Compound:6033012|FD-Score:3.30|P-value:9.05E-4 ID:SGTC_2479|Compound:5617353|FD-Score:-4.72|P-value:5.50E-6 ID:SGTC_2482|Compound:5981709|FD-Score:5.33|P-value:2.54E-7 ID:SGTC_2611|Compound:pyromycin|FD-Score:4.23|P-value:3.28E-5 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-3.76|P-value:2.37E-4 ID:SGTC_2663|Compound:amphotericin b|FD-Score:3.39|P-value:6.74E-4 ID:SGTC_2781|Compound:5567276|FD-Score:4.46|P-value:1.32E-5 ID:SGTC_3127|Compound:9126946|FD-Score:-3.33|P-value:9.91E-4 ID:SGTC_3149|Compound:9097855|FD-Score:-3.58|P-value:4.37E-4

Top fitness defect scores for YDL216C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2026 5148700 8.36 152.0 µM 1.96E-15 2 3.13E-17
2 SGTC_786 4544-0069 6.87 423.0 µM 5.05E-11 6 3.11E-12
3 SGTC_146 3448-8650 6.28 53.1 µM 1.71E-9 NEO1-PIK1 49 1.67E-10
4 SGTC_143 4032-1194 5.97 15.7 µM 9.65E-9 20 1.19E-9
5 SGTC_155 k072-0202 5.66 43.6 µM 4.84E-8 5 7.35E-9
6 SGTC_2482 5981709 5.33 142.7 µM 2.54E-7 PDR1 16 4.78E-8
7 SGTC_785 1270-0111 5.24 65.3 µM 3.96E-7 7 7.90E-8
8 SGTC_1137 ixabepilone 5.10 100.0 µM 7.78E-7 11 1.69E-7
9 SGTC_920 mycophenolic acid 4.75 780.4 nM 3.79E-6 10 1.01E-6
10 SGTC_2028 5153890 4.74 36.4 µM 3.92E-6 cell wall signaling 20 1.05E-6
11 SGTC_121 0557-0495 4.54 61.3 µM 9.54E-6 calcium & mitochondrial duress 42 2.86E-6
12 SGTC_2781 5567276 4.46 71.4 µM 1.32E-5 32 4.14E-6
13 SGTC_1955 wogonin 4.27 32.4 µM 2.89E-5 14 9.97E-6
14 SGTC_2611 pyromycin 4.23 23.9 µM 3.28E-5 19 1.15E-5
15 SGTC_516 3992-2719 4.19 87.3 µM 3.89E-5 13 1.39E-5
16 SGTC_947 3447-0010 4.19 211.0 µM 3.89E-5 9 1.40E-5
17 SGTC_722 3534-0461 4.14 190.0 µM 4.76E-5 PDR1 31 1.75E-5
18 SGTC_1879 gemcitabine 4.02 100.0 µM 7.45E-5 11 2.90E-5
19 SGTC_1292 0kpi-0043 3.89 232.0 µM 1.21E-4 14 5.01E-5
20 SGTC_151 3013-0144 3.86 2.3 µM 1.33E-4 iron homeostasis 31 5.57E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.110 1.58E-10 YDL096C_d OPI6_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene PMT1/YDL095W; YDL096C is not essential
0.103 2.32E-9 YNL246W VPS75 hom NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress
0.097 1.64E-8 YDL033C SLM3 hom tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF)
0.097 2.17E-8 YFL050C ALR2 hom Probable Mg(2+) transporter; overexpression confers increased tolerance to Al(3+) and Ga(3+) ions; plays a role in regulating Ty1 transposition
0.093 6.77E-8 YML062C MFT1 hom Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance
0.089 2.70E-7 YBR101C FES1 hom Hsp70 (Ssa1p) nucleotide exchange factor; cytosolic homolog of Sil1p, which is the nucleotide exchange factor for BiP (Kar2p) in the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
0.085 8.26E-7 YFL022C FRS2 het Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar
0.084 1.06E-6 YEL041W YEF1 hom ATP-NADH kinase; phosphorylates both NAD and NADH; homooctameric structure consisting of 60-kDa subunits; similar to Pos5p; overexpression complements certain pos5 phenotypes; YEF1 has a paralog, UTR1, that arose from the whole genome duplication
0.083 1.66E-6 YDL095W PMT1 hom Protein O-mannosyltransferase of the ER membrane; transfers mannose from dolichyl phosphate-D-mannose to protein serine and threonine residues; 1 of 7 related proteins involved in O-glycosylation which is essential for cell wall rigidity; involved in ER quality control; amino terminus faces cytoplasm, carboxyl terminus faces ER lumen
0.080 3.22E-6 YHR185C PFS1 hom Sporulation protein required for prospore membrane formation at selected spindle poles, ensures functionality of all four spindle pole bodies during meiosis II; not required for meiotic recombination or meiotic chromosome segregation
0.078 5.81E-6 YDL210W UGA4 hom Permease that serves as a gamma-aminobutyrate (GABA) transport protein involved in the utilization of GABA as a nitrogen source; catalyzes the transport of putrescine and delta-aminolevulinic acid (ALA); localized to the vacuolar membrane
0.078 6.63E-6 YDR372C VPS74 hom Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33
0.078 7.09E-6 YBL099W ATP1 hom Alpha subunit of the F1 sector of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated
0.077 8.80E-6 YGR151C_d YGR151C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF RSR1/BUD1/YGR152C; relative distribution to the nucleus increases upon DNA replication stress
0.076 1.19E-5 YLR120C YPS1 hom Aspartic protease, member of the yapsin family of proteases involved in cell wall growth and maintenance; attached to the plasma membrane via a glycosylphosphatidylinositol (GPI) anchor