YDL217C / TIM22

Essential core component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; forms the channel through which proteins are imported

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDL217C / TIM22

Click on Significant Values for Screen Details ID:SGTC_10|Compound:0279-0212|FD-Score:3.14|P-value:5.14E-4 ID:SGTC_63|Compound:0099-0242|FD-Score:5.01|P-value:8.35E-8 ID:SGTC_173|Compound:1273-0061|FD-Score:4.55|P-value:1.02E-6 ID:SGTC_204|Compound:1543-0184|FD-Score:5.04|P-value:6.95E-8 ID:SGTC_235|Compound:0330-0087|FD-Score:3.11|P-value:5.89E-4 ID:SGTC_252|Compound:miconazole|FD-Score:-4.84|P-value:2.16E-7 ID:SGTC_437|Compound:s-farnesyl-l-cysteine methyl ester|FD-Score:-3.15|P-value:5.03E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:4.62|P-value:6.87E-7 ID:SGTC_522|Compound:1273-0010|FD-Score:2.97|P-value:9.71E-4 ID:SGTC_601|Compound:0986-0029|FD-Score:3.36|P-value:2.29E-4 ID:SGTC_674|Compound:0498-0004|FD-Score:4.06|P-value:1.14E-5 ID:SGTC_908|Compound:3448-7491|FD-Score:8.36|P-value:1.28E-18 ID:SGTC_969|Compound:1313-0238|FD-Score:4.96|P-value:1.10E-7 ID:SGTC_1057|Compound:3448-5381|FD-Score:3.19|P-value:4.35E-4 ID:SGTC_1301|Compound:naphtanilide LB|FD-Score:3.65|P-value:6.86E-5 ID:SGTC_1310|Compound:1188-0297|FD-Score:3.68|P-value:6.06E-5 ID:SGTC_1347|Compound:1493-0251|FD-Score:5.22|P-value:2.44E-8 ID:SGTC_1537|Compound:2',6'-dihydroxyflavanone|FD-Score:4.08|P-value:1.02E-5 ID:SGTC_1556|Compound:plumbagin|FD-Score:3.00|P-value:8.77E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:3.28|P-value:3.02E-4 ID:SGTC_1575|Compound:cyproterone acetate|FD-Score:3.32|P-value:2.67E-4 ID:SGTC_1602|Compound:st001878|FD-Score:-3.26|P-value:3.38E-4 ID:SGTC_1608|Compound:st001473|FD-Score:3.20|P-value:4.17E-4 ID:SGTC_1609|Compound:st001574|FD-Score:-3.55|P-value:1.03E-4 ID:SGTC_1616|Compound:st002734|FD-Score:-3.21|P-value:4.01E-4 ID:SGTC_1739|Compound:st037196|FD-Score:3.21|P-value:3.95E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:6.71|P-value:1.19E-12 ID:SGTC_1829|Compound:berberine|FD-Score:3.28|P-value:3.12E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:3.17|P-value:4.64E-4 ID:SGTC_1931|Compound:st074438|FD-Score:3.37|P-value:2.20E-4 ID:SGTC_1933|Compound:st074711|FD-Score:3.33|P-value:2.49E-4 ID:SGTC_1957|Compound:st077035|FD-Score:3.51|P-value:1.21E-4 ID:SGTC_1967|Compound:st070177|FD-Score:3.31|P-value:2.69E-4 ID:SGTC_2057|Compound:5236571|FD-Score:-3.18|P-value:4.48E-4 ID:SGTC_2093|Compound:5331342|FD-Score:5.04|P-value:6.92E-8 ID:SGTC_2139|Compound:5578692|FD-Score:6.03|P-value:1.52E-10 ID:SGTC_2183|Compound:6420625|FD-Score:3.01|P-value:8.21E-4 ID:SGTC_2206|Compound:7254556|FD-Score:3.66|P-value:6.49E-5 ID:SGTC_2223|Compound:6903751|FD-Score:3.10|P-value:5.92E-4 ID:SGTC_2256|Compound:7954252|FD-Score:4.14|P-value:7.50E-6 ID:SGTC_2326|Compound:6164455|FD-Score:3.54|P-value:1.07E-4 ID:SGTC_2356|Compound:9061756|FD-Score:3.05|P-value:7.35E-4 ID:SGTC_2370|Compound:9071495|FD-Score:6.26|P-value:3.05E-11 ID:SGTC_2373|Compound:9071805|FD-Score:3.41|P-value:1.82E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:3.25|P-value:3.49E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:6.35|P-value:1.67E-11 ID:SGTC_2471|Compound:5466956|FD-Score:4.29|P-value:3.69E-6 ID:SGTC_2477|Compound:5967686|FD-Score:-3.83|P-value:3.15E-5 ID:SGTC_2483|Compound:5729050|FD-Score:4.02|P-value:1.35E-5 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-11.50|P-value:2.23E-33 ID:SGTC_2659|Compound:sanguinarine|FD-Score:5.60|P-value:2.45E-9 ID:SGTC_2699|Compound:st077704|FD-Score:-4.48|P-value:1.47E-6 ID:SGTC_2700|Compound:st077718|FD-Score:3.39|P-value:1.97E-4 ID:SGTC_2703|Compound:st077852|FD-Score:-3.55|P-value:1.05E-4 ID:SGTC_2726|Compound:cimetidine|FD-Score:-3.63|P-value:7.45E-5 ID:SGTC_2727|Compound:riluzole|FD-Score:3.17|P-value:4.70E-4 ID:SGTC_2757|Compound:clomiphene|FD-Score:3.39|P-value:1.96E-4 ID:SGTC_2811|Compound:7967822|FD-Score:4.01|P-value:1.40E-5 ID:SGTC_2841|Compound:9004761|FD-Score:3.62|P-value:7.98E-5 ID:SGTC_2858|Compound:9025781|FD-Score:3.84|P-value:3.07E-5 ID:SGTC_2928|Compound:9011484|FD-Score:8.14|P-value:9.39E-18 ID:SGTC_2980|Compound:9014342|FD-Score:11.90|P-value:6.85E-36 ID:SGTC_3017|Compound:9080681|FD-Score:3.15|P-value:5.00E-4 ID:SGTC_3028|Compound:9087778|FD-Score:-5.35|P-value:1.13E-8 ID:SGTC_3029|Compound:9088683|FD-Score:-3.31|P-value:2.74E-4 ID:SGTC_3030|Compound:9087619|FD-Score:-3.42|P-value:1.76E-4 ID:SGTC_3141|Compound:9094609|FD-Score:4.27|P-value:4.17E-6 ID:SGTC_3181|Compound:9108277|FD-Score:5.38|P-value:9.63E-9 ID:SGTC_3205|Compound:9113483|FD-Score:3.85|P-value:2.91E-5 ID:SGTC_3208|Compound:9114115|FD-Score:4.68|P-value:5.21E-7 ID:SGTC_3322|Compound:9139058|FD-Score:-3.01|P-value:8.47E-4 ID:SGTC_10|Compound:0279-0212|FD-Score:3.14|P-value:5.14E-4 ID:SGTC_63|Compound:0099-0242|FD-Score:5.01|P-value:8.35E-8 ID:SGTC_173|Compound:1273-0061|FD-Score:4.55|P-value:1.02E-6 ID:SGTC_204|Compound:1543-0184|FD-Score:5.04|P-value:6.95E-8 ID:SGTC_235|Compound:0330-0087|FD-Score:3.11|P-value:5.89E-4 ID:SGTC_252|Compound:miconazole|FD-Score:-4.84|P-value:2.16E-7 ID:SGTC_437|Compound:s-farnesyl-l-cysteine methyl ester|FD-Score:-3.15|P-value:5.03E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:4.62|P-value:6.87E-7 ID:SGTC_522|Compound:1273-0010|FD-Score:2.97|P-value:9.71E-4 ID:SGTC_601|Compound:0986-0029|FD-Score:3.36|P-value:2.29E-4 ID:SGTC_674|Compound:0498-0004|FD-Score:4.06|P-value:1.14E-5 ID:SGTC_908|Compound:3448-7491|FD-Score:8.36|P-value:1.28E-18 ID:SGTC_969|Compound:1313-0238|FD-Score:4.96|P-value:1.10E-7 ID:SGTC_1057|Compound:3448-5381|FD-Score:3.19|P-value:4.35E-4 ID:SGTC_1301|Compound:naphtanilide LB|FD-Score:3.65|P-value:6.86E-5 ID:SGTC_1310|Compound:1188-0297|FD-Score:3.68|P-value:6.06E-5 ID:SGTC_1347|Compound:1493-0251|FD-Score:5.22|P-value:2.44E-8 ID:SGTC_1537|Compound:2',6'-dihydroxyflavanone|FD-Score:4.08|P-value:1.02E-5 ID:SGTC_1556|Compound:plumbagin|FD-Score:3.00|P-value:8.77E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:3.28|P-value:3.02E-4 ID:SGTC_1575|Compound:cyproterone acetate|FD-Score:3.32|P-value:2.67E-4 ID:SGTC_1602|Compound:st001878|FD-Score:-3.26|P-value:3.38E-4 ID:SGTC_1608|Compound:st001473|FD-Score:3.20|P-value:4.17E-4 ID:SGTC_1609|Compound:st001574|FD-Score:-3.55|P-value:1.03E-4 ID:SGTC_1616|Compound:st002734|FD-Score:-3.21|P-value:4.01E-4 ID:SGTC_1739|Compound:st037196|FD-Score:3.21|P-value:3.95E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:6.71|P-value:1.19E-12 ID:SGTC_1829|Compound:berberine|FD-Score:3.28|P-value:3.12E-4 ID:SGTC_1873|Compound:rapamycin|FD-Score:3.17|P-value:4.64E-4 ID:SGTC_1931|Compound:st074438|FD-Score:3.37|P-value:2.20E-4 ID:SGTC_1933|Compound:st074711|FD-Score:3.33|P-value:2.49E-4 ID:SGTC_1957|Compound:st077035|FD-Score:3.51|P-value:1.21E-4 ID:SGTC_1967|Compound:st070177|FD-Score:3.31|P-value:2.69E-4 ID:SGTC_2057|Compound:5236571|FD-Score:-3.18|P-value:4.48E-4 ID:SGTC_2093|Compound:5331342|FD-Score:5.04|P-value:6.92E-8 ID:SGTC_2139|Compound:5578692|FD-Score:6.03|P-value:1.52E-10 ID:SGTC_2183|Compound:6420625|FD-Score:3.01|P-value:8.21E-4 ID:SGTC_2206|Compound:7254556|FD-Score:3.66|P-value:6.49E-5 ID:SGTC_2223|Compound:6903751|FD-Score:3.10|P-value:5.92E-4 ID:SGTC_2256|Compound:7954252|FD-Score:4.14|P-value:7.50E-6 ID:SGTC_2326|Compound:6164455|FD-Score:3.54|P-value:1.07E-4 ID:SGTC_2356|Compound:9061756|FD-Score:3.05|P-value:7.35E-4 ID:SGTC_2370|Compound:9071495|FD-Score:6.26|P-value:3.05E-11 ID:SGTC_2373|Compound:9071805|FD-Score:3.41|P-value:1.82E-4 ID:SGTC_2408|Compound:sulbentine|FD-Score:3.25|P-value:3.49E-4 ID:SGTC_2418|Compound:pentamidine|FD-Score:6.35|P-value:1.67E-11 ID:SGTC_2471|Compound:5466956|FD-Score:4.29|P-value:3.69E-6 ID:SGTC_2477|Compound:5967686|FD-Score:-3.83|P-value:3.15E-5 ID:SGTC_2483|Compound:5729050|FD-Score:4.02|P-value:1.35E-5 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:-11.50|P-value:2.23E-33 ID:SGTC_2659|Compound:sanguinarine|FD-Score:5.60|P-value:2.45E-9 ID:SGTC_2699|Compound:st077704|FD-Score:-4.48|P-value:1.47E-6 ID:SGTC_2700|Compound:st077718|FD-Score:3.39|P-value:1.97E-4 ID:SGTC_2703|Compound:st077852|FD-Score:-3.55|P-value:1.05E-4 ID:SGTC_2726|Compound:cimetidine|FD-Score:-3.63|P-value:7.45E-5 ID:SGTC_2727|Compound:riluzole|FD-Score:3.17|P-value:4.70E-4 ID:SGTC_2757|Compound:clomiphene|FD-Score:3.39|P-value:1.96E-4 ID:SGTC_2811|Compound:7967822|FD-Score:4.01|P-value:1.40E-5 ID:SGTC_2841|Compound:9004761|FD-Score:3.62|P-value:7.98E-5 ID:SGTC_2858|Compound:9025781|FD-Score:3.84|P-value:3.07E-5 ID:SGTC_2928|Compound:9011484|FD-Score:8.14|P-value:9.39E-18 ID:SGTC_2980|Compound:9014342|FD-Score:11.90|P-value:6.85E-36 ID:SGTC_3017|Compound:9080681|FD-Score:3.15|P-value:5.00E-4 ID:SGTC_3028|Compound:9087778|FD-Score:-5.35|P-value:1.13E-8 ID:SGTC_3029|Compound:9088683|FD-Score:-3.31|P-value:2.74E-4 ID:SGTC_3030|Compound:9087619|FD-Score:-3.42|P-value:1.76E-4 ID:SGTC_3141|Compound:9094609|FD-Score:4.27|P-value:4.17E-6 ID:SGTC_3181|Compound:9108277|FD-Score:5.38|P-value:9.63E-9 ID:SGTC_3205|Compound:9113483|FD-Score:3.85|P-value:2.91E-5 ID:SGTC_3208|Compound:9114115|FD-Score:4.68|P-value:5.21E-7 ID:SGTC_3322|Compound:9139058|FD-Score:-3.01|P-value:8.47E-4

Top fitness defect scores for YDL217C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2980 9014342 11.90 25.3 µM 6.85E-36 1 4.48E-33 1.57
2 SGTC_908 3448-7491 8.36 47.7 µM 1.28E-18 mitochondrial stress 1 3.18E-17 1.59
3 SGTC_2928 9011484 8.14 9.8 µM 9.39E-18 heme biosynthesis & mitochondrial translocase 3 1.97E-16 0.33
4 SGTC_1785 bathophenanthroline 6.71 24.0 µM 1.19E-12 heme requiring 6 9.55E-12 0.42
5 SGTC_2418 pentamidine 6.35 31.7 µM 1.67E-11 mitochondrial processes 6 1.08E-10 0.28
6 SGTC_2370 9071495 6.26 200.0 µM 3.05E-11 mitochondrial processes 2 1.88E-10 1.31
7 SGTC_2139 5578692 6.03 56.5 µM 1.52E-10 superoxide 7 8.22E-10 1.35
8 SGTC_2659 sanguinarine 5.60 24.2 µM 2.45E-9 5 1.06E-8 0.80
9 SGTC_3181 9108277 5.38 49.5 µM 9.63E-9 iron homeostasis 4 3.72E-8 0.13
10 SGTC_1347 1493-0251 5.22 600.0 nM 2.44E-8 mitochondrial response to ROS 2 8.76E-8 0.56
11 SGTC_2093 5331342 5.04 194.4 µM 6.92E-8 2 2.28E-7 0.70
12 SGTC_204 1543-0184 5.04 62.4 µM 6.95E-8 TRP & mitochondrial translation 5 2.29E-7 0.47
13 SGTC_63 0099-0242 5.01 26.7 µM 8.35E-8 mitochondrial stress 14 2.71E-7 0.09
14 SGTC_969 1313-0238 4.96 134.0 µM 1.10E-7 mitochondrial processes 12 3.49E-7 0.30
15 SGTC_3208 9114115 4.68 49.5 µM 5.21E-7 4 1.46E-6 0.06
16 SGTC_451 chloral hydrate 4.62 100.0 µM 6.87E-7 13 1.88E-6 0.02
17 SGTC_173 1273-0061 4.55 2.7 µM 1.02E-6 mitochondrial stress 17 2.72E-6 0.05
18 SGTC_2471 5466956 4.29 24.8 µM 3.69E-6 mitochondrial processes 12 8.84E-6 0.05
19 SGTC_3141 9094609 4.27 49.5 µM 4.17E-6 iron homeostasis 11 9.91E-6 0.05
20 SGTC_2256 7954252 4.14 200.0 µM 7.50E-6 mitochondrial processes 9 1.70E-5 0.29

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.222 7.79E-39 YBR192W RIM2 het Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
0.220 3.52E-38 YER066W_p RRT13_p hom Putative protein of unknown function; non-essential gene identified in a screen for mutants with decreased levels of rDNA transcription
0.198 5.46E-31 YPL266W DIM1 het Essential 18S rRNA dimethylase (dimethyladenosine transferase), responsible for conserved m6(2)Am6(2)A dimethylation in 3'-terminal loop of 18S rRNA, part of 90S and 40S pre-particles in nucleolus, involved in pre-ribosomal RNA processing
0.191 6.05E-29 YLR395C COX8 hom Subunit VIII of cytochrome c oxidase, which is the terminal member of the mitochondrial inner membrane electron transport chain
0.187 7.03E-28 YGL146C_p RRT6_p hom Putative protein of unknown function; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription; contains two putative transmembrane spans, but no significant homology to other known proteins
0.175 1.88E-24 YGR015C_p YGR015C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion
0.173 5.41E-24 YMR113W FOL3 het Dihydrofolate synthetase, involved in folic acid biosynthesis; catalyzes the conversion of dihydropteroate to dihydrofolate in folate coenzyme biosynthesis
0.149 3.13E-18 YNL220W ADE12 hom Adenylosuccinate synthase, catalyzes the first step in synthesis of adenosine monophosphate from inosine 5'monophosphate during purine nucleotide biosynthesis; exhibits binding to single-stranded autonomously replicating (ARS) core sequence
0.148 7.29E-18 YMR203W TOM40 het Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; constitutes the core element of the protein conducting pore
0.144 5.74E-17 YGL068W MNP1 het Protein associated with the mitochondrial nucleoid; putative mitochondrial ribosomal protein with similarity to E. coli L7/L12 ribosomal protein; required for normal respiratory growth
0.143 6.54E-17 YJL043W_p YJL043W_p hom Putative protein of unknown function; YJL043W is a non-essential gene
0.132 1.48E-14 YEL049W PAU2 hom Member of the seripauperin multigene family encoded mainly in subtelomeric regions, active during alcoholic fermentation, regulated by anaerobiosis, negatively regulated by oxygen, repressed by heme
0.131 2.36E-14 YOR300W_d YOR300W_d hom Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7
0.128 9.71E-14 YKL195W MIA40 het Oxidoreductase that mediates mitochondrial intermembrane space import; substrates include small proteins containing twin cysteine motifs; acts in concert with Erv1p, which oxidizes the cysteine residues of Mia40p to comprise a disulfide relay system that catalyzes import; also mediates folding of Atp23p
0.128 1.06E-13 YKR079C TRZ1 het tRNA 3'-end processing endonuclease tRNase Z; also localized to mitochondria and interacts genetically with Rex2 exonuclease; homolog of the human candidate prostate cancer susceptibility gene ELAC2