YDL227C / HO

Site-specific endonuclease required for gene conversion at the MAT locus (homothallic switching) through the generation of a ds DNA break; expression restricted to mother cells in late G1 as controlled by Swi4p-Swi6p, Swi5p and Ash1p

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL227C / HO

Click on Significant Values for Screen Details ID:SGTC_143|Compound:4032-1194|FD-Score:2.69|P-value:7.30E-4 ID:SGTC_144|Compound:4161-2749|FD-Score:-3.81|P-value:3.15E-6 ID:SGTC_148|Compound:3253-0744|FD-Score:-3.50|P-value:1.65E-5 ID:SGTC_152|Compound:4469-0003|FD-Score:5.47|P-value:4.85E-11 ID:SGTC_155|Compound:k072-0202|FD-Score:6.34|P-value:3.12E-14 ID:SGTC_156|Compound:3474-0145|FD-Score:-3.10|P-value:1.18E-4 ID:SGTC_279|Compound:1273-0055|FD-Score:2.69|P-value:7.52E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:2.74|P-value:5.91E-4 ID:SGTC_357|Compound:1325-0123|FD-Score:3.26|P-value:5.90E-5 ID:SGTC_448|Compound:monastrol|FD-Score:-3.00|P-value:1.86E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:-2.69|P-value:7.06E-4 ID:SGTC_518|Compound:1021-0119|FD-Score:-3.10|P-value:1.21E-4 ID:SGTC_753|Compound:1322-0200|FD-Score:2.90|P-value:3.03E-4 ID:SGTC_839|Compound:0122-0028|FD-Score:3.01|P-value:1.90E-4 ID:SGTC_863|Compound:0466-0191|FD-Score:5.82|P-value:2.90E-12 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.09|P-value:1.29E-4 ID:SGTC_1218|Compound:1348-1416|FD-Score:-2.68|P-value:7.34E-4 ID:SGTC_1230|Compound:0170-0365|FD-Score:2.96|P-value:2.37E-4 ID:SGTC_1235|Compound:benzylparaben|FD-Score:2.76|P-value:5.51E-4 ID:SGTC_1301|Compound:naphtanilide LB|FD-Score:2.99|P-value:2.06E-4 ID:SGTC_1302|Compound:1159-0335|FD-Score:-3.08|P-value:1.34E-4 ID:SGTC_1306|Compound:1185-0270|FD-Score:3.09|P-value:1.31E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-6.04|P-value:4.12E-13 ID:SGTC_1463|Compound:k081-0032|FD-Score:-6.11|P-value:2.33E-13 ID:SGTC_1617|Compound:st002906|FD-Score:-3.08|P-value:1.30E-4 ID:SGTC_1686|Compound:st019653|FD-Score:-2.84|P-value:3.76E-4 ID:SGTC_1700|Compound:st030840|FD-Score:3.35|P-value:3.75E-5 ID:SGTC_1703|Compound:st026983|FD-Score:2.74|P-value:6.08E-4 ID:SGTC_1731|Compound:st035964|FD-Score:3.86|P-value:2.55E-6 ID:SGTC_1736|Compound:st036774|FD-Score:3.67|P-value:7.25E-6 ID:SGTC_2112|Compound:5488546|FD-Score:2.85|P-value:3.71E-4 ID:SGTC_2122|Compound:5258151|FD-Score:2.97|P-value:2.24E-4 ID:SGTC_2246|Compound:7293507|FD-Score:2.65|P-value:8.85E-4 ID:SGTC_2293|Compound:7624732|FD-Score:-3.14|P-value:9.89E-5 ID:SGTC_2485|Compound:5633444|FD-Score:-2.82|P-value:4.16E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:4.38|P-value:1.09E-7 ID:SGTC_2619|Compound:obliquin|FD-Score:3.97|P-value:1.38E-6 ID:SGTC_2664|Compound:lyso-paf C-16|FD-Score:-2.70|P-value:6.87E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:3.07|P-value:1.42E-4 ID:SGTC_2786|Compound:7743617|FD-Score:-2.63|P-value:9.03E-4 ID:SGTC_2796|Compound:7949634|FD-Score:-2.78|P-value:5.01E-4 ID:SGTC_2826|Compound:7992541|FD-Score:-2.62|P-value:9.53E-4 ID:SGTC_2965|Compound:9082772|FD-Score:2.90|P-value:3.05E-4 ID:SGTC_2986|Compound:9022445|FD-Score:2.62|P-value:9.68E-4 ID:SGTC_2989|Compound:9073649|FD-Score:4.41|P-value:9.07E-8 ID:SGTC_2991|Compound:9074669|FD-Score:4.60|P-value:2.77E-8 ID:SGTC_3004|Compound:9076122|FD-Score:2.64|P-value:8.96E-4 ID:SGTC_3094|Compound:9117742|FD-Score:-3.39|P-value:3.01E-5 ID:SGTC_3228|Compound:9131799|FD-Score:3.79|P-value:3.80E-6 ID:SGTC_3311|Compound:9131146|FD-Score:6.85|P-value:2.73E-16 ID:SGTC_3350|Compound:9150499|FD-Score:2.67|P-value:8.15E-4 ID:SGTC_3351|Compound:9150312|FD-Score:2.65|P-value:8.88E-4 ID:SGTC_143|Compound:4032-1194|FD-Score:2.69|P-value:7.30E-4 ID:SGTC_144|Compound:4161-2749|FD-Score:-3.81|P-value:3.15E-6 ID:SGTC_148|Compound:3253-0744|FD-Score:-3.50|P-value:1.65E-5 ID:SGTC_152|Compound:4469-0003|FD-Score:5.47|P-value:4.85E-11 ID:SGTC_155|Compound:k072-0202|FD-Score:6.34|P-value:3.12E-14 ID:SGTC_156|Compound:3474-0145|FD-Score:-3.10|P-value:1.18E-4 ID:SGTC_279|Compound:1273-0055|FD-Score:2.69|P-value:7.52E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:2.74|P-value:5.91E-4 ID:SGTC_357|Compound:1325-0123|FD-Score:3.26|P-value:5.90E-5 ID:SGTC_448|Compound:monastrol|FD-Score:-3.00|P-value:1.86E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:-2.69|P-value:7.06E-4 ID:SGTC_518|Compound:1021-0119|FD-Score:-3.10|P-value:1.21E-4 ID:SGTC_753|Compound:1322-0200|FD-Score:2.90|P-value:3.03E-4 ID:SGTC_839|Compound:0122-0028|FD-Score:3.01|P-value:1.90E-4 ID:SGTC_863|Compound:0466-0191|FD-Score:5.82|P-value:2.90E-12 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.09|P-value:1.29E-4 ID:SGTC_1218|Compound:1348-1416|FD-Score:-2.68|P-value:7.34E-4 ID:SGTC_1230|Compound:0170-0365|FD-Score:2.96|P-value:2.37E-4 ID:SGTC_1235|Compound:benzylparaben|FD-Score:2.76|P-value:5.51E-4 ID:SGTC_1301|Compound:naphtanilide LB|FD-Score:2.99|P-value:2.06E-4 ID:SGTC_1302|Compound:1159-0335|FD-Score:-3.08|P-value:1.34E-4 ID:SGTC_1306|Compound:1185-0270|FD-Score:3.09|P-value:1.31E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-6.04|P-value:4.12E-13 ID:SGTC_1463|Compound:k081-0032|FD-Score:-6.11|P-value:2.33E-13 ID:SGTC_1617|Compound:st002906|FD-Score:-3.08|P-value:1.30E-4 ID:SGTC_1686|Compound:st019653|FD-Score:-2.84|P-value:3.76E-4 ID:SGTC_1700|Compound:st030840|FD-Score:3.35|P-value:3.75E-5 ID:SGTC_1703|Compound:st026983|FD-Score:2.74|P-value:6.08E-4 ID:SGTC_1731|Compound:st035964|FD-Score:3.86|P-value:2.55E-6 ID:SGTC_1736|Compound:st036774|FD-Score:3.67|P-value:7.25E-6 ID:SGTC_2112|Compound:5488546|FD-Score:2.85|P-value:3.71E-4 ID:SGTC_2122|Compound:5258151|FD-Score:2.97|P-value:2.24E-4 ID:SGTC_2246|Compound:7293507|FD-Score:2.65|P-value:8.85E-4 ID:SGTC_2293|Compound:7624732|FD-Score:-3.14|P-value:9.89E-5 ID:SGTC_2485|Compound:5633444|FD-Score:-2.82|P-value:4.16E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:4.38|P-value:1.09E-7 ID:SGTC_2619|Compound:obliquin|FD-Score:3.97|P-value:1.38E-6 ID:SGTC_2664|Compound:lyso-paf C-16|FD-Score:-2.70|P-value:6.87E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:3.07|P-value:1.42E-4 ID:SGTC_2786|Compound:7743617|FD-Score:-2.63|P-value:9.03E-4 ID:SGTC_2796|Compound:7949634|FD-Score:-2.78|P-value:5.01E-4 ID:SGTC_2826|Compound:7992541|FD-Score:-2.62|P-value:9.53E-4 ID:SGTC_2965|Compound:9082772|FD-Score:2.90|P-value:3.05E-4 ID:SGTC_2986|Compound:9022445|FD-Score:2.62|P-value:9.68E-4 ID:SGTC_2989|Compound:9073649|FD-Score:4.41|P-value:9.07E-8 ID:SGTC_2991|Compound:9074669|FD-Score:4.60|P-value:2.77E-8 ID:SGTC_3004|Compound:9076122|FD-Score:2.64|P-value:8.96E-4 ID:SGTC_3094|Compound:9117742|FD-Score:-3.39|P-value:3.01E-5 ID:SGTC_3228|Compound:9131799|FD-Score:3.79|P-value:3.80E-6 ID:SGTC_3311|Compound:9131146|FD-Score:6.85|P-value:2.73E-16 ID:SGTC_3350|Compound:9150499|FD-Score:2.67|P-value:8.15E-4 ID:SGTC_3351|Compound:9150312|FD-Score:2.65|P-value:8.88E-4

Top fitness defect scores for YDL227C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3311 9131146 6.85 2.9 µM 2.73E-16 cell wall 49 3.71E-12
2 SGTC_155 k072-0202 6.34 43.6 µM 3.12E-14 4 1.12E-10
3 SGTC_863 0466-0191 5.82 129.0 µM 2.90E-12 cell wall 30 2.91E-9
4 SGTC_152 4469-0003 5.47 551.2 µM 4.85E-11 8 2.21E-8
5 SGTC_2991 9074669 4.60 71.4 µM 2.77E-8 azole & statin 42 2.16E-6
6 SGTC_2989 9073649 4.41 71.4 µM 9.07E-8 24 5.10E-6
7 SGTC_2584 androsterone 4.38 48.7 µM 1.09E-7 cell wall 99 5.82E-6
8 SGTC_2619 obliquin 3.97 87.9 µM 1.38E-6 cell wall 145 3.66E-5
9 SGTC_1731 st035964 3.86 63.0 µM 2.55E-6 31 5.71E-5
10 SGTC_3228 9131799 3.79 49.5 µM 3.80E-6 amide catabolism 6 7.63E-5
11 SGTC_1736 st036774 3.67 51.0 µM 7.25E-6 45 1.22E-4
12 SGTC_1700 st030840 3.35 7.9 µM 3.75E-5 PDR1 39 4.03E-4
13 SGTC_357 1325-0123 3.26 420.0 µM 5.90E-5 23 5.61E-4
14 SGTC_1043 0916-0009 3.09 19.3 µM 1.29E-4 62 9.93E-4
15 SGTC_1306 1185-0270 3.09 9.0 µM 1.31E-4 Golgi 79 0.00100
16 SGTC_2766 oltipraz 3.07 83.9 µM 1.42E-4 heme requiring 64 0.00107
17 SGTC_839 0122-0028 3.01 230.0 µM 1.90E-4 RSC complex & mRNA processing 91 0.00132
18 SGTC_1301 naphtanilide LB 2.99 4.8 µM 2.06E-4 heme requiring 41 0.00140
19 SGTC_2122 5258151 2.97 111.7 µM 2.24E-4 64 0.00149
20 SGTC_1230 0170-0365 2.96 3.8 µM 2.37E-4 endomembrane recycling 146 0.00155

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.288 5.26E-65 YDL199C_p YDL199C_p hom Putative transporter, member of the sugar porter family
0.285 1.07E-63 YDL176W YDL176W hom Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene
0.281 7.05E-62 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.276 8.64E-60 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.266 3.99E-55 YNL166C BNI5 hom Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
0.265 6.95E-55 YDL230W PTP1 hom Phosphotyrosine-specific protein phosphatase that dephosphorylates a broad range of substrates in vivo, including Fpr3p; localized to the cytoplasm and the mitochondria; proposed to be a negative regulator of filamentation
0.261 2.04E-53 YJR137C MET5 hom Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by methionine
0.260 6.88E-53 YOR297C TIM18 hom Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane; may mediate assembly or stability of the complex
0.259 2.13E-52 YPR180W AOS1 het Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
0.255 8.34E-51 YDR169C STB3 hom Ribosomal RNA processing element (RRPE)-binding protein; involved in the glucose-induced transition from quiescence to growth; restricted to nucleus in quiescent cells, released into cytoplasm after glucose repletion; binds Sin3p; relative distribution to the nucleus increases upon DNA replication stress
0.254 2.58E-50 YDR521W_d YDR521W_d hom Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol
0.252 8.08E-50 YGR292W MAL12 hom Maltase (alpha-D-glucosidase), inducible protein involved in maltose catabolism; encoded in the MAL1 complex locus; hydrolyzes the disaccharides maltose, turanose, maltotriose, and sucrose
0.251 2.16E-49 YJL099W CHS6 hom Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.249 1.82E-48 YJL159W HSP150 hom O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
0.247 1.02E-47 YGR136W LSB1 hom Protein containing an N-terminal SH3 domain; binds Las17p, which is a homolog of human Wiskott-Aldrich Syndrome protein involved in actin patch assembly and actin polymerization; protein increases in abundance and forms nuclear foci in response to DNA replication stress