YDL231C / BRE4

Zinc finger protein containing five transmembrane domains; null mutant exhibits strongly fragmented vacuoles and sensitivity to brefeldin A, a drug which is known to affect intracellular transport

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL231C / BRE4

Click on Significant Values for Screen Details ID:SGTC_263|Compound:alizarin|FD-Score:3.36|P-value:3.79E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.93|P-value:4.00E-5 ID:SGTC_512|Compound:prima-1|FD-Score:-3.26|P-value:4.72E-4 ID:SGTC_731|Compound:3966-0296|FD-Score:-3.13|P-value:7.32E-4 ID:SGTC_971|Compound:1315-0384|FD-Score:5.10|P-value:1.48E-7 ID:SGTC_1091|Compound:telmisartan|FD-Score:5.26|P-value:6.31E-8 ID:SGTC_1097|Compound:itavastatin|FD-Score:-3.48|P-value:2.06E-4 ID:SGTC_1166|Compound:k206-0078|FD-Score:-3.63|P-value:1.15E-4 ID:SGTC_1167|Compound:k781-0610|FD-Score:-4.50|P-value:2.59E-6 ID:SGTC_1266|Compound:0828-0227|FD-Score:4.05|P-value:2.39E-5 ID:SGTC_1639|Compound:Lupinine o-methylbenzoicacid ester hydrochloride|FD-Score:3.45|P-value:2.77E-4 ID:SGTC_1699|Compound:st031318|FD-Score:3.46|P-value:2.62E-4 ID:SGTC_1703|Compound:st026983|FD-Score:3.10|P-value:9.50E-4 ID:SGTC_1781|Compound:st038725|FD-Score:-3.31|P-value:3.83E-4 ID:SGTC_1846|Compound:st056244|FD-Score:3.11|P-value:9.20E-4 ID:SGTC_1853|Compound:st056710|FD-Score:5.46|P-value:2.06E-8 ID:SGTC_2310|Compound:7668677|FD-Score:3.10|P-value:9.66E-4 ID:SGTC_2333|Compound:9017922|FD-Score:6.24|P-value:1.78E-10 ID:SGTC_2338|Compound:7999974|FD-Score:3.81|P-value:6.66E-5 ID:SGTC_2339|Compound:9000433|FD-Score:3.99|P-value:3.16E-5 ID:SGTC_2394|Compound:5221648|FD-Score:4.21|P-value:1.21E-5 ID:SGTC_2570|Compound:licareol|FD-Score:4.34|P-value:6.61E-6 ID:SGTC_2581|Compound:avocatin a|FD-Score:-3.53|P-value:1.69E-4 ID:SGTC_2585|Compound:alliin|FD-Score:-3.12|P-value:7.52E-4 ID:SGTC_2618|Compound:avocadyne|FD-Score:3.72|P-value:9.44E-5 ID:SGTC_2750|Compound:bromebric acid|FD-Score:3.30|P-value:4.66E-4 ID:SGTC_2759|Compound:disulfiram|FD-Score:4.15|P-value:1.58E-5 ID:SGTC_2780|Compound:5215298|FD-Score:3.47|P-value:2.56E-4 ID:SGTC_2816|Compound:7982706|FD-Score:3.35|P-value:3.94E-4 ID:SGTC_2836|Compound:9010338|FD-Score:3.57|P-value:1.72E-4 ID:SGTC_2905|Compound:7799065|FD-Score:4.45|P-value:3.95E-6 ID:SGTC_2906|Compound:7926292|FD-Score:4.05|P-value:2.41E-5 ID:SGTC_2950|Compound:9072895|FD-Score:-4.91|P-value:3.35E-7 ID:SGTC_2954|Compound:9074818|FD-Score:-3.20|P-value:5.86E-4 ID:SGTC_2959|Compound:9079155|FD-Score:-3.79|P-value:6.06E-5 ID:SGTC_3056|Compound:9094900|FD-Score:3.98|P-value:3.22E-5 ID:SGTC_3153|Compound:9100258|FD-Score:-3.17|P-value:6.32E-4 ID:SGTC_3233|Compound:9132340|FD-Score:3.13|P-value:8.74E-4 ID:SGTC_3259|Compound:9138485|FD-Score:3.10|P-value:9.65E-4 ID:SGTC_3288|Compound:9119690|FD-Score:5.85|P-value:2.10E-9 ID:SGTC_263|Compound:alizarin|FD-Score:3.36|P-value:3.79E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.93|P-value:4.00E-5 ID:SGTC_512|Compound:prima-1|FD-Score:-3.26|P-value:4.72E-4 ID:SGTC_731|Compound:3966-0296|FD-Score:-3.13|P-value:7.32E-4 ID:SGTC_971|Compound:1315-0384|FD-Score:5.10|P-value:1.48E-7 ID:SGTC_1091|Compound:telmisartan|FD-Score:5.26|P-value:6.31E-8 ID:SGTC_1097|Compound:itavastatin|FD-Score:-3.48|P-value:2.06E-4 ID:SGTC_1166|Compound:k206-0078|FD-Score:-3.63|P-value:1.15E-4 ID:SGTC_1167|Compound:k781-0610|FD-Score:-4.50|P-value:2.59E-6 ID:SGTC_1266|Compound:0828-0227|FD-Score:4.05|P-value:2.39E-5 ID:SGTC_1639|Compound:Lupinine o-methylbenzoicacid ester hydrochloride|FD-Score:3.45|P-value:2.77E-4 ID:SGTC_1699|Compound:st031318|FD-Score:3.46|P-value:2.62E-4 ID:SGTC_1703|Compound:st026983|FD-Score:3.10|P-value:9.50E-4 ID:SGTC_1781|Compound:st038725|FD-Score:-3.31|P-value:3.83E-4 ID:SGTC_1846|Compound:st056244|FD-Score:3.11|P-value:9.20E-4 ID:SGTC_1853|Compound:st056710|FD-Score:5.46|P-value:2.06E-8 ID:SGTC_2310|Compound:7668677|FD-Score:3.10|P-value:9.66E-4 ID:SGTC_2333|Compound:9017922|FD-Score:6.24|P-value:1.78E-10 ID:SGTC_2338|Compound:7999974|FD-Score:3.81|P-value:6.66E-5 ID:SGTC_2339|Compound:9000433|FD-Score:3.99|P-value:3.16E-5 ID:SGTC_2394|Compound:5221648|FD-Score:4.21|P-value:1.21E-5 ID:SGTC_2570|Compound:licareol|FD-Score:4.34|P-value:6.61E-6 ID:SGTC_2581|Compound:avocatin a|FD-Score:-3.53|P-value:1.69E-4 ID:SGTC_2585|Compound:alliin|FD-Score:-3.12|P-value:7.52E-4 ID:SGTC_2618|Compound:avocadyne|FD-Score:3.72|P-value:9.44E-5 ID:SGTC_2750|Compound:bromebric acid|FD-Score:3.30|P-value:4.66E-4 ID:SGTC_2759|Compound:disulfiram|FD-Score:4.15|P-value:1.58E-5 ID:SGTC_2780|Compound:5215298|FD-Score:3.47|P-value:2.56E-4 ID:SGTC_2816|Compound:7982706|FD-Score:3.35|P-value:3.94E-4 ID:SGTC_2836|Compound:9010338|FD-Score:3.57|P-value:1.72E-4 ID:SGTC_2905|Compound:7799065|FD-Score:4.45|P-value:3.95E-6 ID:SGTC_2906|Compound:7926292|FD-Score:4.05|P-value:2.41E-5 ID:SGTC_2950|Compound:9072895|FD-Score:-4.91|P-value:3.35E-7 ID:SGTC_2954|Compound:9074818|FD-Score:-3.20|P-value:5.86E-4 ID:SGTC_2959|Compound:9079155|FD-Score:-3.79|P-value:6.06E-5 ID:SGTC_3056|Compound:9094900|FD-Score:3.98|P-value:3.22E-5 ID:SGTC_3153|Compound:9100258|FD-Score:-3.17|P-value:6.32E-4 ID:SGTC_3233|Compound:9132340|FD-Score:3.13|P-value:8.74E-4 ID:SGTC_3259|Compound:9138485|FD-Score:3.10|P-value:9.65E-4 ID:SGTC_3288|Compound:9119690|FD-Score:5.85|P-value:2.10E-9

Top fitness defect scores for YDL231C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2333 9017922 6.24 200.0 µM 1.78E-10 5 2.17E-10
2 SGTC_3288 9119690 5.85 36.2 µM 2.10E-9 10 2.48E-9
3 SGTC_1853 st056710 5.46 91.2 µM 2.06E-8 amide catabolism 6 2.37E-8
4 SGTC_1091 telmisartan 5.26 47.6 µM 6.31E-8 2 7.14E-8
5 SGTC_971 1315-0384 5.10 26.7 µM 1.48E-7 cell wall signaling 6 1.66E-7
6 SGTC_2905 7799065 4.45 20.1 µM 3.95E-6 9 4.25E-6
7 SGTC_2570 licareol 4.34 100.0 µM 6.61E-6 20 7.06E-6
8 SGTC_2394 5221648 4.21 200.0 µM 1.21E-5 19 1.29E-5
9 SGTC_2759 disulfiram 4.15 3.4 µM 1.58E-5 21 1.68E-5
10 SGTC_1266 0828-0227 4.05 32.9 µM 2.39E-5 20 2.51E-5
11 SGTC_2906 7926292 4.05 26.4 µM 2.41E-5 22 2.54E-5
12 SGTC_2339 9000433 3.99 200.0 µM 3.16E-5 38 3.32E-5
13 SGTC_3056 9094900 3.98 49.5 µM 3.22E-5 22 3.38E-5
14 SGTC_453 enantio-paf c16 3.93 10.0 µM 4.00E-5 142 4.19E-5
15 SGTC_2338 7999974 3.81 200.0 µM 6.66E-5 27 6.94E-5
16 SGTC_2618 avocadyne 3.72 43.4 µM 9.44E-5 36 9.79E-5
17 SGTC_2836 9010338 3.57 19.5 µM 1.72E-4 RPP1 & pyrimidine depletion 46 1.78E-4
18 SGTC_2780 5215298 3.47 71.4 µM 2.56E-4 55 2.63E-4
19 SGTC_1699 st031318 3.46 29.1 µM 2.62E-4 34 2.69E-4
20 SGTC_1639 Lupinine o-methylbenzoicacid ester hydrochloride 3.45 61.8 µM 2.77E-4 ergosterol biosynthesis 19 2.85E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.110 1.84E-10 YDR379W RGA2 hom GTPase-activating protein for the polarity-establishment protein Cdc42p; implicated in control of septin organization, pheromone response, and haploid invasive growth; regulated by Pho85p and Cdc28p
0.109 2.67E-10 YKL019W RAM2 het Alpha subunit of both the farnesyltransferase and type I geranylgeranyltransferase that catalyze prenylation of proteins containing a CAAX consensus motif; essential protein required for membrane localization of Ras proteins and a-factor
0.105 1.13E-9 YLR230W_d YLR230W_d het Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential CDC42 gene which encodes a small Rho-like GTPase essential for establishment and maintenance of cell polarity
0.101 5.25E-9 YBL005W PDR3 hom Transcriptional activator of the pleiotropic drug resistance network; regulates expression of ATP-binding cassette (ABC) transporters through binding to cis-acting sites known as PDREs (PDR responsive elements); post-translationally up-regulated in cells lacking a functional mitochondrial genome; PDR3 has a paralog, PDR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.094 5.57E-8 YML101C-A_d YML101C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.092 1.04E-7 YGL089C MF(ALPHA)2 hom Mating pheromone alpha-factor, made by alpha cells; interacts with mating type a cells to induce cell cycle arrest and other responses leading to mating; also encoded by MF(ALPHA)1, which is more highly expressed than MF(ALPHA)2
0.090 1.96E-7 YCR092C MSH3 hom Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability
0.088 3.52E-7 YOR256C TRE2 het Transferrin receptor-like protein; functions with Tre1p to regulate ubiquitylation and vacuolar degradation of the metal transporter Smf1p; inviability of null mutant in systematic studies is due to proximity to CDC31; TRE2 has a paralog, TRE1, that arose from the whole genome duplication
0.087 4.97E-7 YJR148W BAT2 hom Cytosolic branched-chain amino acid (BCAA) aminotransferase, preferentially involved in BCAA catabolism; homolog of murine ECA39; highly expressed during stationary phase and repressed during logarithmic phase
0.085 7.23E-7 YGR024C THG1 het tRNAHis guanylyltransferase, adds a guanosine residue to the 5' end of tRNAHis after transcription and RNase P cleavage; couples nuclear division and migration to cell budding and cytokinesis; essential enzyme conserved among eukaryotes
0.085 8.77E-7 YIL058W_d YIL058W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.082 1.85E-6 YAR031W PRM9 hom Pheromone-regulated protein with 3 predicted transmembrane segments and an FF sequence, a motif involved in COPII binding; member of DUP240 gene family
0.081 3.02E-6 YOR269W PAC1 hom Involved in nuclear migration, part of the dynein/dynactin pathway; targets dynein to microtubule tips, which is necessary for sliding of microtubules along bud cortex; serves at interface between dynein's ATPase site and its microtubule binding stalk, causing individual dynein motors to remain attached to microtubules for long periods; synthetic lethal with bni1; homolog of human LIS1, mutations in which cause the severe brain disorder lissencephaly
0.077 8.25E-6 YDL039C PRM7 hom Pheromone-regulated protein, predicted to have one transmembrane segment; promoter contains Gcn4p binding elements
0.077 9.04E-6 YDR255C RMD5 hom Component of GID Complex that confers ubiquitin ligase (U3) activity; necessary for polyubiquitination and degradation of the gluconeogenic enzyme fructose-1,6-bisphosphatase; forms dimer with Fyv10p that is then recruited to GID Complex by Gid8p; also required for sporulation; conserved protein that has a degenerate RING finger domain