YDL240W / LRG1

Putative GTPase-activating protein (GAP) involved in the Pkc1p-mediated signaling pathway that controls cell wall integrity; appears to specifically regulate 1,3-beta-glucan synthesis

Zygosity: Homozygous strain
fixedexpanded
Profile for YDL240W / LRG1

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:-6.54|P-value:1.95E-11 ID:SGTC_78|Compound:0336-0123|FD-Score:-3.12|P-value:7.96E-4 ID:SGTC_112|Compound:phenothiazine|FD-Score:4.28|P-value:9.10E-6 ID:SGTC_137|Compound:0438-0332|FD-Score:-3.11|P-value:8.10E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:-4.24|P-value:9.04E-6 ID:SGTC_178|Compound:4092-0806|FD-Score:-3.89|P-value:4.11E-5 ID:SGTC_206|Compound:4546-0025|FD-Score:3.68|P-value:1.15E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:3.10|P-value:9.77E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-4.69|P-value:1.04E-6 ID:SGTC_518|Compound:1021-0119|FD-Score:3.90|P-value:4.68E-5 ID:SGTC_519|Compound:k048-0011|FD-Score:3.41|P-value:3.25E-4 ID:SGTC_674|Compound:0498-0004|FD-Score:3.13|P-value:8.57E-4 ID:SGTC_837|Compound:0091-0265|FD-Score:3.64|P-value:1.35E-4 ID:SGTC_840|Compound:0245-0196|FD-Score:3.11|P-value:9.18E-4 ID:SGTC_1001|Compound:4048-4355|FD-Score:4.82|P-value:6.67E-7 ID:SGTC_1053|Compound:3366-3204|FD-Score:-3.18|P-value:6.47E-4 ID:SGTC_1624|Compound:st003710|FD-Score:-4.52|P-value:2.42E-6 ID:SGTC_1632|Compound:st005650|FD-Score:-4.14|P-value:1.41E-5 ID:SGTC_1637|Compound:st007924|FD-Score:5.33|P-value:4.47E-8 ID:SGTC_1638|Compound:st008370|FD-Score:5.35|P-value:4.00E-8 ID:SGTC_1789|Compound:tunicamycin|FD-Score:3.65|P-value:1.29E-4 ID:SGTC_1862|Compound:st057513|FD-Score:4.70|P-value:1.24E-6 ID:SGTC_1865|Compound:st057639|FD-Score:4.19|P-value:1.35E-5 ID:SGTC_1871|Compound:st057448|FD-Score:7.77|P-value:3.10E-15 ID:SGTC_1877|Compound:wortmannin|FD-Score:-5.36|P-value:3.07E-8 ID:SGTC_1962|Compound:st076595|FD-Score:6.27|P-value:1.53E-10 ID:SGTC_1966|Compound:st070145|FD-Score:6.87|P-value:2.67E-12 ID:SGTC_1971|Compound:st069429|FD-Score:-4.07|P-value:1.95E-5 ID:SGTC_2006|Compound:4011487|FD-Score:-3.11|P-value:8.25E-4 ID:SGTC_2273|Compound:7815444|FD-Score:-3.23|P-value:5.43E-4 ID:SGTC_2296|Compound:7971656|FD-Score:3.65|P-value:1.29E-4 ID:SGTC_2366|Compound:9070441|FD-Score:3.96|P-value:3.68E-5 ID:SGTC_2460|Compound:5552655|FD-Score:-4.10|P-value:1.66E-5 ID:SGTC_2469|Compound:5655019|FD-Score:-4.34|P-value:5.63E-6 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:-3.30|P-value:4.10E-4 ID:SGTC_2546|Compound:rhodinyl acetate|FD-Score:3.27|P-value:5.40E-4 ID:SGTC_2653|Compound:dihydrofissinolide|FD-Score:-3.24|P-value:5.09E-4 ID:SGTC_2675|Compound:theaflavin digallate|FD-Score:4.08|P-value:2.15E-5 ID:SGTC_2756|Compound:carbadox|FD-Score:4.08|P-value:2.15E-5 ID:SGTC_2775|Compound:cyproheptadine|FD-Score:3.11|P-value:9.42E-4 ID:SGTC_2909|Compound:7941780|FD-Score:-3.58|P-value:1.43E-4 ID:SGTC_3080|Compound:9117754|FD-Score:-3.14|P-value:7.35E-4 ID:SGTC_3106|Compound:9121286|FD-Score:3.93|P-value:4.20E-5 ID:SGTC_3191|Compound:9110352|FD-Score:5.03|P-value:2.27E-7 ID:SGTC_58|Compound:0957-0393|FD-Score:-6.54|P-value:1.95E-11 ID:SGTC_78|Compound:0336-0123|FD-Score:-3.12|P-value:7.96E-4 ID:SGTC_112|Compound:phenothiazine|FD-Score:4.28|P-value:9.10E-6 ID:SGTC_137|Compound:0438-0332|FD-Score:-3.11|P-value:8.10E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:-4.24|P-value:9.04E-6 ID:SGTC_178|Compound:4092-0806|FD-Score:-3.89|P-value:4.11E-5 ID:SGTC_206|Compound:4546-0025|FD-Score:3.68|P-value:1.15E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:3.10|P-value:9.77E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-4.69|P-value:1.04E-6 ID:SGTC_518|Compound:1021-0119|FD-Score:3.90|P-value:4.68E-5 ID:SGTC_519|Compound:k048-0011|FD-Score:3.41|P-value:3.25E-4 ID:SGTC_674|Compound:0498-0004|FD-Score:3.13|P-value:8.57E-4 ID:SGTC_837|Compound:0091-0265|FD-Score:3.64|P-value:1.35E-4 ID:SGTC_840|Compound:0245-0196|FD-Score:3.11|P-value:9.18E-4 ID:SGTC_1001|Compound:4048-4355|FD-Score:4.82|P-value:6.67E-7 ID:SGTC_1053|Compound:3366-3204|FD-Score:-3.18|P-value:6.47E-4 ID:SGTC_1624|Compound:st003710|FD-Score:-4.52|P-value:2.42E-6 ID:SGTC_1632|Compound:st005650|FD-Score:-4.14|P-value:1.41E-5 ID:SGTC_1637|Compound:st007924|FD-Score:5.33|P-value:4.47E-8 ID:SGTC_1638|Compound:st008370|FD-Score:5.35|P-value:4.00E-8 ID:SGTC_1789|Compound:tunicamycin|FD-Score:3.65|P-value:1.29E-4 ID:SGTC_1862|Compound:st057513|FD-Score:4.70|P-value:1.24E-6 ID:SGTC_1865|Compound:st057639|FD-Score:4.19|P-value:1.35E-5 ID:SGTC_1871|Compound:st057448|FD-Score:7.77|P-value:3.10E-15 ID:SGTC_1877|Compound:wortmannin|FD-Score:-5.36|P-value:3.07E-8 ID:SGTC_1962|Compound:st076595|FD-Score:6.27|P-value:1.53E-10 ID:SGTC_1966|Compound:st070145|FD-Score:6.87|P-value:2.67E-12 ID:SGTC_1971|Compound:st069429|FD-Score:-4.07|P-value:1.95E-5 ID:SGTC_2006|Compound:4011487|FD-Score:-3.11|P-value:8.25E-4 ID:SGTC_2273|Compound:7815444|FD-Score:-3.23|P-value:5.43E-4 ID:SGTC_2296|Compound:7971656|FD-Score:3.65|P-value:1.29E-4 ID:SGTC_2366|Compound:9070441|FD-Score:3.96|P-value:3.68E-5 ID:SGTC_2460|Compound:5552655|FD-Score:-4.10|P-value:1.66E-5 ID:SGTC_2469|Compound:5655019|FD-Score:-4.34|P-value:5.63E-6 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:-3.30|P-value:4.10E-4 ID:SGTC_2546|Compound:rhodinyl acetate|FD-Score:3.27|P-value:5.40E-4 ID:SGTC_2653|Compound:dihydrofissinolide|FD-Score:-3.24|P-value:5.09E-4 ID:SGTC_2675|Compound:theaflavin digallate|FD-Score:4.08|P-value:2.15E-5 ID:SGTC_2756|Compound:carbadox|FD-Score:4.08|P-value:2.15E-5 ID:SGTC_2775|Compound:cyproheptadine|FD-Score:3.11|P-value:9.42E-4 ID:SGTC_2909|Compound:7941780|FD-Score:-3.58|P-value:1.43E-4 ID:SGTC_3080|Compound:9117754|FD-Score:-3.14|P-value:7.35E-4 ID:SGTC_3106|Compound:9121286|FD-Score:3.93|P-value:4.20E-5 ID:SGTC_3191|Compound:9110352|FD-Score:5.03|P-value:2.27E-7

Top fitness defect scores for YDL240W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1871 st057448 7.77 67.3 µM 3.10E-15 1 4.05E-15
2 SGTC_1966 st070145 6.87 16.1 µM 2.67E-12 4 3.24E-12
3 SGTC_1962 st076595 6.27 64.5 µM 1.53E-10 redox potentiating 5 1.78E-10
4 SGTC_1638 st008370 5.35 60.6 µM 4.00E-8 ergosterol biosynthesis 10 4.40E-8
5 SGTC_1637 st007924 5.33 17.4 µM 4.47E-8 8 4.92E-8
6 SGTC_3191 9110352 5.03 49.5 µM 2.27E-7 1 2.45E-7
7 SGTC_1001 4048-4355 4.82 44.4 µM 6.67E-7 copper-dependent oxidative stress 20 7.14E-7
8 SGTC_1862 st057513 4.70 3.8 µM 1.24E-6 3 1.32E-6
9 SGTC_112 phenothiazine 4.28 287.2 µM 9.10E-6 unfolded protein response 9 9.51E-6
10 SGTC_1865 st057639 4.19 52.2 µM 1.35E-5 8 1.41E-5
11 SGTC_2756 carbadox 4.08 75.1 µM 2.15E-5 53 2.23E-5
12 SGTC_2675 theaflavin digallate 4.08 100.0 µM 2.15E-5 iron homeostasis 29 2.23E-5
13 SGTC_2366 9070441 3.96 60.6 µM 3.68E-5 29 3.81E-5
14 SGTC_3106 9121286 3.93 49.5 µM 4.20E-5 12 4.33E-5
15 SGTC_518 1021-0119 3.90 62.6 µM 4.68E-5 ERG2 41 4.82E-5
16 SGTC_206 4546-0025 3.68 72.0 µM 1.15E-4 NEO1 87 1.17E-4
17 SGTC_1789 tunicamycin 3.65 25.0 nM 1.29E-4 unfolded protein response 60 1.32E-4
18 SGTC_2296 7971656 3.65 126.5 µM 1.29E-4 52 1.32E-4
19 SGTC_837 0091-0265 3.64 12.5 µM 1.35E-4 ubiquinone biosynthesis & proteasome 32 1.38E-4
20 SGTC_519 k048-0011 3.41 62.8 µM 3.25E-4 37 3.30E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.130 4.48E-14 YNL219C ALG9 hom Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation
0.119 5.00E-12 YHL001W RPL14B hom Ribosomal 60S subunit protein L14B; homologous to mammalian ribosomal protein L14, no bacterial homolog; RPL14B has a paralog, RPL14A, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.116 1.95E-11 YGR206W MVB12 hom ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin
0.107 4.33E-10 YDR319C_p YFT2_p hom Protein required for normal ER membrane biosynthesis; member of the highly conserved FIT family of proteins involved in triglyceride droplet biosynthesis and homologous to human FIT2; interacts with Sst2p and Hsp82p in high-throughput two-hybrid screens
0.102 3.02E-9 YDR516C EMI2 hom Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.098 1.50E-8 YOR067C ALG8 hom Glucosyl transferase, involved in N-linked glycosylation; adds glucose to the dolichol-linked oligosaccharide precursor prior to transfer to protein during lipid-linked oligosaccharide biosynthesis; similar to Alg6p
0.096 2.53E-8 YLR417W VPS36 hom Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome
0.092 1.04E-7 YBR012C_d YBR012C_d hom Dubious open reading frame, unlikely to encode a functional protein; expression induced by iron-regulated transcriptional activator Aft2p
0.090 1.69E-7 YBR084C-A RPL19A hom Ribosomal 60S subunit protein L19A; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19A has a paralog, RPL19B, that arose from the whole genome duplication
0.090 1.87E-7 YNL242W ATG2 hom Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress
0.090 1.93E-7 YLR307W CDA1 hom Chitin deacetylase, together with Cda2p involved in the biosynthesis ascospore wall component, chitosan; required for proper rigidity of the ascospore wall
0.088 3.07E-7 YPL120W VPS30 hom Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1
0.088 3.14E-7 YNL067W RPL9B hom Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6
0.085 7.07E-7 YDL053C PBP4 hom Pbp1p binding protein; interacts strongly with Pab1p-binding protein 1 (Pbp1p) in the yeast two-hybrid system; also interacts with Lsm12p in a copurification assay; relative distribution to the nucleus increases upon DNA replication stress
0.085 7.23E-7 YOR002W ALG6 hom Alpha 1,3 glucosyltransferase, involved in transfer of oligosaccharides from dolichyl pyrophosphate to asparagine residues of proteins during N-linked protein glycosylation; mutations in human ortholog are associated with disease