YDR001C / NTH1

Neutral trehalase, degrades trehalose; required for thermotolerance and may mediate resistance to other cellular stresses; may be phosphorylated by Cdc28p; NTH1 has a paralog, NTH2, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR001C / NTH1

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:6.79|P-value:6.02E-13 ID:SGTC_70|Compound:0141-0289|FD-Score:3.90|P-value:2.29E-5 ID:SGTC_149|Compound:3970-0822|FD-Score:3.18|P-value:4.48E-4 ID:SGTC_156|Compound:3474-0145|FD-Score:3.27|P-value:3.17E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:9.21|P-value:2.74E-22 ID:SGTC_163|Compound:1327-0036|FD-Score:-3.14|P-value:4.92E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:5.58|P-value:2.53E-9 ID:SGTC_214|Compound:4373-3496|FD-Score:4.36|P-value:2.59E-6 ID:SGTC_252|Compound:miconazole|FD-Score:3.14|P-value:5.23E-4 ID:SGTC_401|Compound:neomycin|FD-Score:3.12|P-value:5.59E-4 ID:SGTC_651|Compound:3253-0966|FD-Score:3.43|P-value:1.65E-4 ID:SGTC_709|Compound:3807-1509|FD-Score:-3.43|P-value:1.55E-4 ID:SGTC_757|Compound:1066-0008|FD-Score:4.73|P-value:3.72E-7 ID:SGTC_768|Compound:0384-0033|FD-Score:3.29|P-value:2.93E-4 ID:SGTC_773|Compound:4358-1457|FD-Score:3.19|P-value:4.21E-4 ID:SGTC_896|Compound:0964-0007|FD-Score:3.67|P-value:6.12E-5 ID:SGTC_927|Compound:2425-1185|FD-Score:4.78|P-value:2.87E-7 ID:SGTC_1080|Compound:nisoldipine|FD-Score:-3.24|P-value:3.31E-4 ID:SGTC_1236|Compound:0330-0013|FD-Score:3.30|P-value:2.83E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:3.13|P-value:5.38E-4 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:3.60|P-value:8.25E-5 ID:SGTC_2134|Compound:5324836|FD-Score:-3.01|P-value:7.88E-4 ID:SGTC_2135|Compound:5326629|FD-Score:-3.64|P-value:6.47E-5 ID:SGTC_2284|Compound:7945075|FD-Score:-3.91|P-value:1.99E-5 ID:SGTC_2329|Compound:6138490|FD-Score:4.26|P-value:4.09E-6 ID:SGTC_2482|Compound:5981709|FD-Score:3.02|P-value:7.83E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:3.18|P-value:4.49E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:4.20|P-value:5.61E-6 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:3.69|P-value:5.76E-5 ID:SGTC_2584|Compound:androsterone|FD-Score:5.92|P-value:2.89E-10 ID:SGTC_2644|Compound:guaiazulene|FD-Score:-2.95|P-value:9.73E-4 ID:SGTC_2693|Compound:st077581|FD-Score:-2.99|P-value:8.32E-4 ID:SGTC_2954|Compound:9074818|FD-Score:-4.41|P-value:1.80E-6 ID:SGTC_2962|Compound:9082311|FD-Score:3.34|P-value:2.41E-4 ID:SGTC_3141|Compound:9094609|FD-Score:3.39|P-value:1.95E-4 ID:SGTC_3168|Compound:9103419|FD-Score:-3.14|P-value:4.88E-4 ID:SGTC_3222|Compound:9128301|FD-Score:-3.06|P-value:6.57E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:6.79|P-value:6.02E-13 ID:SGTC_70|Compound:0141-0289|FD-Score:3.90|P-value:2.29E-5 ID:SGTC_149|Compound:3970-0822|FD-Score:3.18|P-value:4.48E-4 ID:SGTC_156|Compound:3474-0145|FD-Score:3.27|P-value:3.17E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:9.21|P-value:2.74E-22 ID:SGTC_163|Compound:1327-0036|FD-Score:-3.14|P-value:4.92E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:5.58|P-value:2.53E-9 ID:SGTC_214|Compound:4373-3496|FD-Score:4.36|P-value:2.59E-6 ID:SGTC_252|Compound:miconazole|FD-Score:3.14|P-value:5.23E-4 ID:SGTC_401|Compound:neomycin|FD-Score:3.12|P-value:5.59E-4 ID:SGTC_651|Compound:3253-0966|FD-Score:3.43|P-value:1.65E-4 ID:SGTC_709|Compound:3807-1509|FD-Score:-3.43|P-value:1.55E-4 ID:SGTC_757|Compound:1066-0008|FD-Score:4.73|P-value:3.72E-7 ID:SGTC_768|Compound:0384-0033|FD-Score:3.29|P-value:2.93E-4 ID:SGTC_773|Compound:4358-1457|FD-Score:3.19|P-value:4.21E-4 ID:SGTC_896|Compound:0964-0007|FD-Score:3.67|P-value:6.12E-5 ID:SGTC_927|Compound:2425-1185|FD-Score:4.78|P-value:2.87E-7 ID:SGTC_1080|Compound:nisoldipine|FD-Score:-3.24|P-value:3.31E-4 ID:SGTC_1236|Compound:0330-0013|FD-Score:3.30|P-value:2.83E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:3.13|P-value:5.38E-4 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:3.60|P-value:8.25E-5 ID:SGTC_2134|Compound:5324836|FD-Score:-3.01|P-value:7.88E-4 ID:SGTC_2135|Compound:5326629|FD-Score:-3.64|P-value:6.47E-5 ID:SGTC_2284|Compound:7945075|FD-Score:-3.91|P-value:1.99E-5 ID:SGTC_2329|Compound:6138490|FD-Score:4.26|P-value:4.09E-6 ID:SGTC_2482|Compound:5981709|FD-Score:3.02|P-value:7.83E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:3.18|P-value:4.49E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:4.20|P-value:5.61E-6 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:3.69|P-value:5.76E-5 ID:SGTC_2584|Compound:androsterone|FD-Score:5.92|P-value:2.89E-10 ID:SGTC_2644|Compound:guaiazulene|FD-Score:-2.95|P-value:9.73E-4 ID:SGTC_2693|Compound:st077581|FD-Score:-2.99|P-value:8.32E-4 ID:SGTC_2954|Compound:9074818|FD-Score:-4.41|P-value:1.80E-6 ID:SGTC_2962|Compound:9082311|FD-Score:3.34|P-value:2.41E-4 ID:SGTC_3141|Compound:9094609|FD-Score:3.39|P-value:1.95E-4 ID:SGTC_3168|Compound:9103419|FD-Score:-3.14|P-value:4.88E-4 ID:SGTC_3222|Compound:9128301|FD-Score:-3.06|P-value:6.57E-4

Top fitness defect scores for YDR001C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_159 3970-0959 9.21 50.0 µM 2.74E-22 6 1.71E-20
2 SGTC_58 0957-0393 6.79 9.0 µM 6.02E-13 cell wall 171 5.72E-12
3 SGTC_2584 androsterone 5.92 48.7 µM 2.89E-10 cell wall 61 1.61E-9
4 SGTC_212 3807-4682 5.58 38.8 µM 2.53E-9 tubulin folding & SWR complex 31 1.17E-8
5 SGTC_927 2425-1185 4.78 123.0 µM 2.87E-7 10 8.84E-7
6 SGTC_757 1066-0008 4.73 322.0 µM 3.72E-7 Golgi 9 1.12E-6
7 SGTC_214 4373-3496 4.36 70.0 µM 2.59E-6 46 6.62E-6
8 SGTC_2329 6138490 4.26 200.0 µM 4.09E-6 21 1.01E-5
9 SGTC_2563 tomatine 4.20 8.5 µM 5.61E-6 35 1.34E-5
10 SGTC_70 0141-0289 3.90 43.3 µM 2.29E-5 mitochondrial stress 179 4.87E-5
11 SGTC_2571 chrysanthemic acid 3.69 100.0 µM 5.76E-5 31 1.13E-4
12 SGTC_896 0964-0007 3.67 44.5 µM 6.12E-5 18 1.20E-4
13 SGTC_1592 4',5'-dihydroxyflavone 3.60 8.8 µM 8.25E-5 60S ribosome export 22 1.58E-4
14 SGTC_651 3253-0966 3.43 34.4 µM 1.65E-4 calcium & mitochondrial duress 51 2.99E-4
15 SGTC_3141 9094609 3.39 49.5 µM 1.95E-4 iron homeostasis 56 3.48E-4
16 SGTC_2962 9082311 3.34 13.2 µM 2.41E-4 42 4.21E-4
17 SGTC_1236 0330-0013 3.30 258.0 µM 2.83E-4 32 4.88E-4
18 SGTC_768 0384-0033 3.29 77.3 µM 2.93E-4 fatty acid desaturase (OLE1) 121 5.05E-4
19 SGTC_156 3474-0145 3.27 5.6 µM 3.17E-4 endomembrane recycling 79 5.42E-4
20 SGTC_773 4358-1457 3.19 44.6 µM 4.21E-4 68 7.03E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.241 1.48E-45 YDR049W VMS1 hom Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans
0.205 3.58E-33 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.204 5.37E-33 YCL005W LDB16 hom Protein of unknown function; null mutants have decreased net negative cell surface charge; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; native protein is detected in purified mitochondria
0.203 1.27E-32 YKL076C_d PSY1_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C
0.198 4.74E-31 YDR446W ECM11 hom Non-essential protein apparently involved in meiosis, GFP fusion protein is present in discrete clusters in the nucleus throughout mitosis; may be involved in maintaining chromatin structure
0.195 3.41E-30 YOR091W TMA46 hom Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p
0.193 2.16E-29 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.188 4.68E-28 YOL054W PSH1 hom E3 ubiquitin ligase that mediates poyubiquitination and degradation of centromere-binding protein Cse4p and prevents Cse4p from mislocalizing to euchromatin; ubiquitylation of Cse4p may be antagonized by Scm3p
0.184 5.20E-27 YMR111C YMR111C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the nucleus; YMR111C is not an essential gene; forms nuclear foci upon DNA replication stress
0.182 2.05E-26 YJL020C BBC1 hom Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches
0.182 1.95E-26 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.181 4.00E-26 YGL033W HOP2 hom Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair
0.179 2.02E-25 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.178 2.81E-25 YDL162C_d YDL162C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps ENT1/YDL161W, a verified gene involved in endocytosis and actin cortical patch assembly
0.177 4.70E-25 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate