YDR002W / YRB1

Ran GTPase binding protein; involved in nuclear protein import and RNA export, ubiquitin-mediated protein degradation during the cell cycle; shuttles between the nucleus and cytoplasm; is essential; homolog of human RanBP1

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR002W / YRB1

Click on Significant Values for Screen Details ID:SGTC_27|Compound:0485-0001|FD-Score:-3.95|P-value:1.02E-4 ID:SGTC_41|Compound:0478-0632|FD-Score:-3.41|P-value:6.75E-4 ID:SGTC_81|Compound:0411-0046|FD-Score:4.68|P-value:4.74E-6 ID:SGTC_96|Compound:0929-0093|FD-Score:3.60|P-value:3.27E-4 ID:SGTC_99|Compound:0109-0120|FD-Score:-3.46|P-value:5.58E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:7.84|P-value:5.96E-14 ID:SGTC_904|Compound:1141-0088|FD-Score:3.33|P-value:7.89E-4 ID:SGTC_947|Compound:3447-0010|FD-Score:3.55|P-value:3.90E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:4.64|P-value:5.65E-6 ID:SGTC_960|Compound:1222-0186|FD-Score:3.49|P-value:4.76E-4 ID:SGTC_988|Compound:1497-1057|FD-Score:-3.40|P-value:7.00E-4 ID:SGTC_1063|Compound:disulfiram|FD-Score:3.77|P-value:1.80E-4 ID:SGTC_1071|Compound:trifluoperazine|FD-Score:4.02|P-value:6.98E-5 ID:SGTC_1093|Compound:fluphenazine|FD-Score:3.46|P-value:5.16E-4 ID:SGTC_1150|Compound:3909-7983|FD-Score:3.85|P-value:1.34E-4 ID:SGTC_1512|Compound:0259-0544|FD-Score:3.56|P-value:3.73E-4 ID:SGTC_1711|Compound:st032263|FD-Score:3.95|P-value:9.08E-5 ID:SGTC_1783|Compound:st039459|FD-Score:5.19|P-value:4.51E-7 ID:SGTC_1790|Compound:st045711|FD-Score:-3.32|P-value:9.01E-4 ID:SGTC_1804|Compound:st050076|FD-Score:4.85|P-value:2.22E-6 ID:SGTC_1811|Compound:st050069|FD-Score:-3.43|P-value:6.36E-4 ID:SGTC_1820|Compound:st053765|FD-Score:-5.57|P-value:7.53E-8 ID:SGTC_1857|Compound:st056007|FD-Score:3.52|P-value:4.20E-4 ID:SGTC_2305|Compound:7443522|FD-Score:3.31|P-value:8.57E-4 ID:SGTC_2338|Compound:7999974|FD-Score:3.75|P-value:1.94E-4 ID:SGTC_2534|Compound:helenine|FD-Score:3.48|P-value:4.82E-4 ID:SGTC_2578|Compound:7,8-dihydroxyflavone|FD-Score:-4.12|P-value:5.19E-5 ID:SGTC_2584|Compound:androsterone|FD-Score:-4.19|P-value:4.06E-5 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:3.80|P-value:1.58E-4 ID:SGTC_2940|Compound:9060638|FD-Score:-3.79|P-value:1.82E-4 ID:SGTC_3210|Compound:9128157|FD-Score:4.41|P-value:1.49E-5 ID:SGTC_27|Compound:0485-0001|FD-Score:-3.95|P-value:1.02E-4 ID:SGTC_41|Compound:0478-0632|FD-Score:-3.41|P-value:6.75E-4 ID:SGTC_81|Compound:0411-0046|FD-Score:4.68|P-value:4.74E-6 ID:SGTC_96|Compound:0929-0093|FD-Score:3.60|P-value:3.27E-4 ID:SGTC_99|Compound:0109-0120|FD-Score:-3.46|P-value:5.58E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:7.84|P-value:5.96E-14 ID:SGTC_904|Compound:1141-0088|FD-Score:3.33|P-value:7.89E-4 ID:SGTC_947|Compound:3447-0010|FD-Score:3.55|P-value:3.90E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:4.64|P-value:5.65E-6 ID:SGTC_960|Compound:1222-0186|FD-Score:3.49|P-value:4.76E-4 ID:SGTC_988|Compound:1497-1057|FD-Score:-3.40|P-value:7.00E-4 ID:SGTC_1063|Compound:disulfiram|FD-Score:3.77|P-value:1.80E-4 ID:SGTC_1071|Compound:trifluoperazine|FD-Score:4.02|P-value:6.98E-5 ID:SGTC_1093|Compound:fluphenazine|FD-Score:3.46|P-value:5.16E-4 ID:SGTC_1150|Compound:3909-7983|FD-Score:3.85|P-value:1.34E-4 ID:SGTC_1512|Compound:0259-0544|FD-Score:3.56|P-value:3.73E-4 ID:SGTC_1711|Compound:st032263|FD-Score:3.95|P-value:9.08E-5 ID:SGTC_1783|Compound:st039459|FD-Score:5.19|P-value:4.51E-7 ID:SGTC_1790|Compound:st045711|FD-Score:-3.32|P-value:9.01E-4 ID:SGTC_1804|Compound:st050076|FD-Score:4.85|P-value:2.22E-6 ID:SGTC_1811|Compound:st050069|FD-Score:-3.43|P-value:6.36E-4 ID:SGTC_1820|Compound:st053765|FD-Score:-5.57|P-value:7.53E-8 ID:SGTC_1857|Compound:st056007|FD-Score:3.52|P-value:4.20E-4 ID:SGTC_2305|Compound:7443522|FD-Score:3.31|P-value:8.57E-4 ID:SGTC_2338|Compound:7999974|FD-Score:3.75|P-value:1.94E-4 ID:SGTC_2534|Compound:helenine|FD-Score:3.48|P-value:4.82E-4 ID:SGTC_2578|Compound:7,8-dihydroxyflavone|FD-Score:-4.12|P-value:5.19E-5 ID:SGTC_2584|Compound:androsterone|FD-Score:-4.19|P-value:4.06E-5 ID:SGTC_2588|Compound:indole-3-carbinol|FD-Score:3.80|P-value:1.58E-4 ID:SGTC_2940|Compound:9060638|FD-Score:-3.79|P-value:1.82E-4 ID:SGTC_3210|Compound:9128157|FD-Score:4.41|P-value:1.49E-5

Top fitness defect scores for YDR002W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_168 3970-0862 7.84 530.5 µM 5.96E-14 9 2.20E-15 0.23
2 SGTC_1783 st039459 5.19 87.5 µM 4.51E-7 Golgi 4 1.06E-7 0.12
3 SGTC_1804 st050076 4.85 49.1 µM 2.22E-6 2 6.25E-7 0.72
4 SGTC_81 0411-0046 4.68 10.0 µM 4.74E-6 30 1.45E-6 0.10
5 SGTC_951 1171-0579 4.64 82.7 µM 5.65E-6 cell wall 15 1.77E-6 0.01
6 SGTC_3210 9128157 4.41 49.5 µM 1.49E-5 8 5.20E-6 0.05
7 SGTC_1071 trifluoperazine 4.02 6.3 µM 6.98E-5 8 2.90E-5 0.41
8 SGTC_1711 st032263 3.95 62.8 µM 9.08E-5 3 3.89E-5 0.54
9 SGTC_1150 3909-7983 3.85 10.9 µM 1.34E-4 5 5.99E-5 0.16
10 SGTC_2588 indole-3-carbinol 3.80 100.0 µM 1.58E-4 9 7.18E-5 0.02
11 SGTC_1063 disulfiram 3.77 1.8 µM 1.80E-4 10 8.32E-5 0.08
12 SGTC_2338 7999974 3.75 200.0 µM 1.94E-4 10 9.01E-5 0.01
13 SGTC_96 0929-0093 3.60 37.3 µM 3.27E-4 calcium & mitochondrial duress 6 1.61E-4 0.21
14 SGTC_1512 0259-0544 3.56 12.2 µM 3.73E-4 8 1.86E-4 0.20
15 SGTC_947 3447-0010 3.55 211.0 µM 3.90E-4 6 1.96E-4 0.13
16 SGTC_1857 st056007 3.52 79.3 µM 4.20E-4 3 2.13E-4 0.25
17 SGTC_960 1222-0186 3.49 7.7 µM 4.76E-4 14 2.45E-4 0.06
18 SGTC_2534 helenine 3.48 20.1 µM 4.82E-4 RSC complex & mRNA processing 28 2.48E-4 0.12
19 SGTC_1093 fluphenazine 3.46 8.0 µM 5.16E-4 9 2.68E-4 0.16
20 SGTC_904 1141-0088 3.33 396.0 µM 7.89E-4 5 4.28E-4 0.15

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.200 1.41E-31 YJL101C GSH1 hom Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress
0.154 2.58E-19 YOL049W GSH2 hom Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock
0.136 3.71E-15 YDR088C SLU7 het RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain
0.131 2.53E-14 YFL033C RIM15 hom Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase
0.124 5.31E-13 YDR005C MAF1 hom Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions
0.116 1.81E-11 YDR189W SLY1 het Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex
0.110 1.97E-10 YDL108W KIN28 het Serine/threonine protein kinase, subunit of the transcription factor TFIIH; involved in transcription initiation at RNA polymerase II promoters
0.103 2.03E-9 YHR202W_p YHR202W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole, while HA-tagged protein is found in the soluble fraction, suggesting cytoplasmic localization
0.098 1.46E-8 YHR205W SCH9 hom AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan
0.096 2.34E-8 YBR290W BSD2 hom Heavy metal ion homeostasis protein, facilitates trafficking of Smf1p and Smf2p metal transporters to the vacuole where they are degraded, controls metal ion transport, prevents metal hyperaccumulation, functions in copper detoxification
0.096 2.76E-8 YBR008C FLR1 hom Plasma membrane transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; involved in efflux of fluconazole, diazaborine, benomyl, methotrexate, and other drugs; expression induced in cells treated with the mycotoxin patulin; relocalizes from nucleus to plasma membrane upon DNA replication stress
0.093 7.39E-8 YDR069C DOA4 hom Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole
0.089 2.75E-7 YIL026C IRR1 het Subunit of the cohesin complex, which is required for sister chromatid cohesion during mitosis and meiosis and interacts with centromeres and chromosome arms, essential for viability
0.088 3.00E-7 YBR193C MED8 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
0.088 3.98E-7 YNL181W_p YNL181W_p het Putative oxidoreductase; required for cell viability