HIPHOP chemogenomics database

YDR005C / MAF1

Highly conserved negative regulator of RNA polymerase III; binds to the N-terminal domain of the Rpc160p subunit of Pol III to prevent closed-complex formation; localization and activity are regulated by phosphorylation, mediated by TORC1, protein kinase A, and Sch9p; localizes to cytoplasm during vegetative growth and translocates to the nucleus and nucleolus under stress conditions

Zygosity: Homozygous strain

fixedexpanded
Profile for YDR005C / MAF1

Click on Significant Values for Screen Details

Top fitness defect scores for YDR005C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_168	3970-0862	14.40	530.5 µM	1.04E-39		3	3.00E-47
2	SGTC_159	3970-0959	11.10	50.0 µM	1.57E-24		4	4.88E-29
3	SGTC_163	1327-0036	7.99	7.5 µM	1.40E-13		20	6.48E-16
4	SGTC_59	0330-0160	6.73	1.3 µM	3.81E-10		25	8.31E-12
5	SGTC_1131	3454-3128	6.01	32.3 µM	2.00E-8		5	9.45E-10
6	SGTC_214	4373-3496	5.85	70.0 µM	4.38E-8		14	2.41E-9
7	SGTC_1286	0929-0070	5.53	131.0 µM	2.19E-7		7	1.65E-8
8	SGTC_87	0100-0052	4.90	35.5 µM	3.73E-6		58	4.82E-7
9	SGTC_3131	9127911	4.81	49.5 µM	5.48E-6		12	7.62E-7
10	SGTC_615	k087-0205	4.72	167.0 µM	7.97E-6		8	1.19E-6
11	SGTC_2159	5658611	4.63	73.8 µM	1.13E-5		4	1.80E-6
12	SGTC_166	k072-0058	4.61	166.4 µM	1.22E-5	DNA intercalators	20	1.97E-6
13	SGTC_734	2023-0417	4.44	80.0 µM	2.44E-5	ergosterol biosynthesis	7	4.50E-6
14	SGTC_2149	5554786	4.38	194.8 µM	3.07E-5	cell wall signaling	25	5.94E-6
15	SGTC_993	3909-8757	4.24	62.7 µM	5.21E-5		19	1.11E-5
16	SGTC_743	1275-0778	4.20	158.0 µM	6.15E-5	excess fatty acid	30	1.35E-5
17	SGTC_1184	1269-1718	4.07	139.0 µM	9.83E-5		24	2.36E-5
18	SGTC_1675	st018485	3.99	24.5 µM	1.29E-4	60S ribosome export	27	3.28E-5
19	SGTC_2404	5161160	3.85	200.0 µM	2.12E-4		23	5.90E-5
20	SGTC_1732	st034308	3.84	51.8 µM	2.18E-4		28	6.09E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.278	2.82E-60	YJL101C	GSH1	hom	Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress
0.248	2.69E-48	YFL033C	RIM15	hom	Glucose-repressible protein kinase involved in signal transduction during cell proliferation in response to nutrients, specifically the establishment of stationary phase; identified as a regulator of IME2; substrate of Pho80p-Pho85p kinase
0.219	1.31E-37	YOL049W	GSH2	hom	Glutathione synthetase, catalyzes the ATP-dependent synthesis of glutathione (GSH) from gamma-glutamylcysteine and glycine; induced by oxidative stress and heat shock
0.214	6.04E-36	YKL193C	SDS22	het	Regulatory subunit of the type 1 protein phosphatase (PP1) Glc7p; whether it functions as a positive or negative regulator of Glc7p is controversial; involved in the regulation of Glc7p nuclear localization and function
0.211	3.14E-35	YHR205W	SCH9	hom	AGC family protein kinase; functional ortholog of mammalian S6 kinase; phosphorylated by Tor1p and required for TORC1-mediated regulation of ribosome biogenesis, translation initiation, and entry into G0 phase; involved in transactivation of osmostress-responsive genes; regulates G1 progression, cAPK activity and nitrogen activation of the FGM pathway; integrates nutrient signals and stress signals from sphingolipids to regulate lifespan
0.206	1.61E-33	YDR069C	DOA4	hom	Ubiquitin isopeptidase, required for recycling ubiquitin from proteasome-bound ubiquitinated intermediates, acts at the late endosome/prevacuolar compartment to recover ubiquitin from ubiquitinated membrane proteins en route to the vacuole
0.202	3.73E-32	YJR013W	GPI14	het	Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M
0.200	1.60E-31	YDR049W	VMS1	hom	Component of a Cdc48p-complex involved in protein quality control; exhibits cytosolic and ER-membrane localization, with Cdc48p, during normal growth, and contributes to ER-associated degradation (ERAD) of specific substrates at a step after their ubiquitination; forms a mitochondrially-associated complex with Cdc48p and Npl4p under oxidative stress that is required for ubiquitin-mediated mitochondria-associated protein degradation (MAD); conserved in C. elegans and humans
0.178	2.99E-25	YJL202C_d	YJL202C_d	het	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; overlaps 3' end of essential PRP21 gene encoding a subunit of the SF3a splicing factor complex
0.171	2.45E-23	YJR139C	HOM6	hom	Homoserine dehydrogenase (L-homoserine:NADP oxidoreductase), dimeric enzyme that catalyzes the third step in the common pathway for methionine and threonine biosynthesis; enzyme has nucleotide-binding, dimerization and catalytic regions
0.169	6.29E-23	YIR008C	PRI1	het	Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
0.164	1.46E-21	YHR025W	THR1	hom	Homoserine kinase, conserved protein required for threonine biosynthesis; expression is regulated by the GCN4-mediated general amino acid control pathway
0.163	2.37E-21	YGR193C	PDX1	hom	Dihydrolipoamide dehydrogenase (E3)-binding protein (E3BP) of the mitochondrial pyruvate dehydrogenase (PDH) complex, plays a structural role in the complex by binding and positioning E3 to the dihydrolipoamide acetyltransferase (E2) core
0.156	9.36E-20	YDR088C	SLU7	het	RNA splicing factor, required for ATP-independent portion of 2nd catalytic step of spliceosomal RNA splicing; interacts with Prp18p; contains zinc knuckle domain
0.150	2.88E-18	YDL189W	RBS1	hom	Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain