HIPHOP chemogenomics database

YDR007W / TRP1

Phosphoribosylanthranilate isomerase that catalyzes the third step in tryptophan biosynthesis; in 2004, the sequence of TRP1 from strain S228C was updated by changing the previously annotated internal STOP (TAA) to serine (TCA)

Zygosity: Homozygous strain

fixedexpanded
Profile for YDR007W / TRP1

Click on Significant Values for Screen Details

Top fitness defect scores for YDR007W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_129	0088-0017	27.90	176.6 µM	5.53E-52		1	2.31E-171
2	SGTC_2607	solasodine	27.30	47.4 µM	6.27E-50		4	1.97E-164
3	SGTC_1883	5-benzylidenerhodanine	25.40	20.0 µM	1.84E-43	TRP & mitochondrial translation	1	1.28E-142
4	SGTC_2488	5268135	25.10	45.7 µM	1.44E-42		2	1.32E-139
5	SGTC_2384	9036245	24.00	200.0 µM	4.41E-39		1	7.58E-128
6	SGTC_3308	9128332	23.60	17.6 µM	1.19E-37	plasma membrane duress	4	5.06E-123
7	SGTC_2038	4100038	22.90	31.0 µM	8.85E-36	plasma membrane duress	4	1.04E-116
8	SGTC_1882	5615643	22.40	20.0 µM	3.90E-34	TRP & mitochondrial translation	1	3.65E-111
9	SGTC_2758	dehydroepiandrosterone	21.80	69.3 µM	2.29E-32		5	3.32E-105
10	SGTC_2618	avocadyne	21.40	43.4 µM	2.64E-31		5	1.27E-101
11	SGTC_2540	epiandrosterone	19.90	100.0 µM	2.34E-27		1	2.55E-88
12	SGTC_2137	5261020	19.80	140.0 µM	3.96E-27		1	1.50E-87
13	SGTC_180	1486-1293	19.60	102.8 µM	1.46E-26		3	1.21E-85
14	SGTC_1715	st032160	19.20	10.2 µM	1.06E-25		1	9.81E-83
15	SGTC_1881	5652484	19.20	16.0 µM	1.21E-25	TRP & mitochondrial translation	1	1.50E-82
16	SGTC_2232	chlorophetanol	19.20	41.5 µM	1.31E-25	TRP & mitochondrial translation	5	1.96E-82
17	SGTC_2254	7938975	18.90	95.2 µM	7.82E-25		4	8.12E-80
18	SGTC_1999	st074831	18.30	12.5 µM	2.39E-23	plasma membrane duress	1	8.20E-75
19	SGTC_2939	9044578	18.20	71.4 µM	2.65E-23		4	1.15E-74
20	SGTC_1636	st007707	18.20	68.9 µM	3.42E-23		2	2.74E-74

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.753	0	YDR008C_d	YDR008C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.739	0	YDR354W	TRP4	hom	Anthranilate phosphoribosyl transferase of the tryptophan biosynthetic pathway, catalyzes the phosphoribosylation of anthranilate, subject to the general control system of amino acid biosynthesis
0.704	0	YKL211C	TRP3	hom	Indole-3-glycerol-phosphate synthase; forms bifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp2p
0.696	0	YGL026C	TRP5	hom	Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis
0.679	0	YEL009C	GCN4	hom	Basic leucine zipper (bZIP) transcriptional activator of amino acid biosynthetic genes in response to amino acid starvation; expression is tightly regulated at both the transcriptional and translational levels
0.655	0	YER090W	TRP2	hom	Anthranilate synthase, catalyzes the initial step of tryptophan biosynthesis, forms multifunctional hetero-oligomeric anthranilate synthase:indole-3-glycerol phosphate synthase enzyme complex with Trp3p
0.629	0	YDR283C	GCN2	hom	Protein kinase, phosphorylates the alpha-subunit of translation initiation factor eIF2 (Sui2p) in response to starvation; activated by uncharged tRNAs and the Gcn1p-Gcn20p complex; contributes to DNA damage checkpoint control
0.609	0	YKR026C	GCN3	hom	Alpha subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a positive regulator of GCN4 expression
0.607	0	YDR127W	ARO1	hom	Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
0.532	8.59E-244	YDR035W	ARO3	hom	3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase, catalyzes the first step in aromatic amino acid biosynthesis and is feedback-inhibited by phenylalanine or high concentration of tyrosine or tryptophan
0.515	6.07E-226	YGL117W_p	YGL117W_p	hom	Putative protein of unknown function
0.489	2.55E-201	YFR009W	GCN20	hom	Positive regulator of the Gcn2p kinase activity, forms a complex with Gcn1p; proposed to stimulate Gcn2p activation by an uncharged tRNA
0.394	4.71E-125	YGL148W	ARO2	hom	Bifunctional chorismate synthase and flavin reductase; catalyzes the conversion of 5-enolpyruvylshikimate 3-phosphate (EPSP) to form chorismate, which is a precursor to aromatic amino acids; protein abundance increases in response to DNA replication stress
0.200	1.29E-31	YIR023W	DAL81	hom	Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
0.189	1.87E-28	YDL226C	GCS1	hom	ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p