YDR036C / EHD3

3-hydroxyisobutyryl-CoA hydrolase, member of a family of enoyl-CoA hydratase/isomerases; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; phosphorylated; mutation affects fluid-phase endocytosis

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR036C / EHD3

Click on Significant Values for Screen Details ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:2.86|P-value:7.39E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.20|P-value:1.91E-4 ID:SGTC_535|Compound:2293-4129|FD-Score:3.75|P-value:1.50E-5 ID:SGTC_554|Compound:4483-2211|FD-Score:-2.94|P-value:4.71E-4 ID:SGTC_695|Compound:3505-6186|FD-Score:2.84|P-value:8.10E-4 ID:SGTC_725|Compound:1497-0963|FD-Score:2.91|P-value:6.28E-4 ID:SGTC_726|Compound:3448-4391|FD-Score:4.14|P-value:2.12E-6 ID:SGTC_732|Compound:3970-1727|FD-Score:3.21|P-value:1.78E-4 ID:SGTC_757|Compound:1066-0008|FD-Score:3.16|P-value:2.25E-4 ID:SGTC_759|Compound:3486-0358|FD-Score:2.80|P-value:9.54E-4 ID:SGTC_770|Compound:0389-1121|FD-Score:3.05|P-value:3.55E-4 ID:SGTC_828|Compound:2074-0507|FD-Score:2.89|P-value:6.76E-4 ID:SGTC_1006|Compound:4099-6547|FD-Score:2.96|P-value:5.11E-4 ID:SGTC_1027|Compound:k015-0025|FD-Score:-4.03|P-value:3.12E-6 ID:SGTC_1044|Compound:1222-0021|FD-Score:3.29|P-value:1.27E-4 ID:SGTC_1070|Compound:indatraline|FD-Score:2.90|P-value:6.41E-4 ID:SGTC_1089|Compound:terbinafine|FD-Score:2.97|P-value:4.94E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.22|P-value:1.09E-6 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:-2.95|P-value:4.67E-4 ID:SGTC_1242|Compound:0402-0103|FD-Score:-2.84|P-value:7.02E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:3.22|P-value:1.76E-4 ID:SGTC_1737|Compound:st035557|FD-Score:-3.78|P-value:1.12E-5 ID:SGTC_1808|Compound:st050063|FD-Score:3.87|P-value:8.63E-6 ID:SGTC_1826|Compound:st055655|FD-Score:-4.15|P-value:1.60E-6 ID:SGTC_1907|Compound:st060210|FD-Score:2.86|P-value:7.56E-4 ID:SGTC_1909|Compound:st060837|FD-Score:-3.65|P-value:2.15E-5 ID:SGTC_1919|Compound:st067113|FD-Score:3.23|P-value:1.65E-4 ID:SGTC_2425|Compound:5628681|FD-Score:3.24|P-value:1.57E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.42|P-value:7.08E-5 ID:SGTC_2568|Compound:diosmetin|FD-Score:3.62|P-value:2.83E-5 ID:SGTC_2610|Compound:ononetin|FD-Score:-2.79|P-value:8.46E-4 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-2.84|P-value:7.20E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:4.54|P-value:2.21E-7 ID:SGTC_2678|Compound:equilin|FD-Score:3.03|P-value:3.77E-4 ID:SGTC_2694|Compound:st077685|FD-Score:-3.00|P-value:3.77E-4 ID:SGTC_2785|Compound:7756463|FD-Score:-6.19|P-value:2.02E-12 ID:SGTC_2789|Compound:5281112|FD-Score:-5.65|P-value:1.25E-10 ID:SGTC_2806|Compound:7952793|FD-Score:3.57|P-value:3.69E-5 ID:SGTC_2841|Compound:9004761|FD-Score:-3.17|P-value:1.88E-4 ID:SGTC_2940|Compound:9060638|FD-Score:2.88|P-value:7.05E-4 ID:SGTC_3010|Compound:9080089|FD-Score:-2.77|P-value:9.14E-4 ID:SGTC_3210|Compound:9128157|FD-Score:-2.98|P-value:4.05E-4 ID:SGTC_3236|Compound:9132701|FD-Score:2.86|P-value:7.43E-4 ID:SGTC_3242|Compound:9133940|FD-Score:3.23|P-value:1.65E-4 ID:SGTC_3258|Compound:9138477|FD-Score:-3.41|P-value:6.49E-5 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:2.86|P-value:7.39E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.20|P-value:1.91E-4 ID:SGTC_535|Compound:2293-4129|FD-Score:3.75|P-value:1.50E-5 ID:SGTC_554|Compound:4483-2211|FD-Score:-2.94|P-value:4.71E-4 ID:SGTC_695|Compound:3505-6186|FD-Score:2.84|P-value:8.10E-4 ID:SGTC_725|Compound:1497-0963|FD-Score:2.91|P-value:6.28E-4 ID:SGTC_726|Compound:3448-4391|FD-Score:4.14|P-value:2.12E-6 ID:SGTC_732|Compound:3970-1727|FD-Score:3.21|P-value:1.78E-4 ID:SGTC_757|Compound:1066-0008|FD-Score:3.16|P-value:2.25E-4 ID:SGTC_759|Compound:3486-0358|FD-Score:2.80|P-value:9.54E-4 ID:SGTC_770|Compound:0389-1121|FD-Score:3.05|P-value:3.55E-4 ID:SGTC_828|Compound:2074-0507|FD-Score:2.89|P-value:6.76E-4 ID:SGTC_1006|Compound:4099-6547|FD-Score:2.96|P-value:5.11E-4 ID:SGTC_1027|Compound:k015-0025|FD-Score:-4.03|P-value:3.12E-6 ID:SGTC_1044|Compound:1222-0021|FD-Score:3.29|P-value:1.27E-4 ID:SGTC_1070|Compound:indatraline|FD-Score:2.90|P-value:6.41E-4 ID:SGTC_1089|Compound:terbinafine|FD-Score:2.97|P-value:4.94E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-4.22|P-value:1.09E-6 ID:SGTC_1135|Compound:dihydrocapsaicin|FD-Score:-2.95|P-value:4.67E-4 ID:SGTC_1242|Compound:0402-0103|FD-Score:-2.84|P-value:7.02E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:3.22|P-value:1.76E-4 ID:SGTC_1737|Compound:st035557|FD-Score:-3.78|P-value:1.12E-5 ID:SGTC_1808|Compound:st050063|FD-Score:3.87|P-value:8.63E-6 ID:SGTC_1826|Compound:st055655|FD-Score:-4.15|P-value:1.60E-6 ID:SGTC_1907|Compound:st060210|FD-Score:2.86|P-value:7.56E-4 ID:SGTC_1909|Compound:st060837|FD-Score:-3.65|P-value:2.15E-5 ID:SGTC_1919|Compound:st067113|FD-Score:3.23|P-value:1.65E-4 ID:SGTC_2425|Compound:5628681|FD-Score:3.24|P-value:1.57E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.42|P-value:7.08E-5 ID:SGTC_2568|Compound:diosmetin|FD-Score:3.62|P-value:2.83E-5 ID:SGTC_2610|Compound:ononetin|FD-Score:-2.79|P-value:8.46E-4 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:-2.84|P-value:7.20E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:4.54|P-value:2.21E-7 ID:SGTC_2678|Compound:equilin|FD-Score:3.03|P-value:3.77E-4 ID:SGTC_2694|Compound:st077685|FD-Score:-3.00|P-value:3.77E-4 ID:SGTC_2785|Compound:7756463|FD-Score:-6.19|P-value:2.02E-12 ID:SGTC_2789|Compound:5281112|FD-Score:-5.65|P-value:1.25E-10 ID:SGTC_2806|Compound:7952793|FD-Score:3.57|P-value:3.69E-5 ID:SGTC_2841|Compound:9004761|FD-Score:-3.17|P-value:1.88E-4 ID:SGTC_2940|Compound:9060638|FD-Score:2.88|P-value:7.05E-4 ID:SGTC_3010|Compound:9080089|FD-Score:-2.77|P-value:9.14E-4 ID:SGTC_3210|Compound:9128157|FD-Score:-2.98|P-value:4.05E-4 ID:SGTC_3236|Compound:9132701|FD-Score:2.86|P-value:7.43E-4 ID:SGTC_3242|Compound:9133940|FD-Score:3.23|P-value:1.65E-4 ID:SGTC_3258|Compound:9138477|FD-Score:-3.41|P-value:6.49E-5

Top fitness defect scores for YDR036C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2640 pentamidine 4.54 10.4 µM 2.21E-7 mitochondrial processes 18 2.82E-6
2 SGTC_726 3448-4391 4.14 34.2 µM 2.12E-6 9 1.77E-5
3 SGTC_1808 st050063 3.87 61.5 µM 8.63E-6 12 5.54E-5
4 SGTC_535 2293-4129 3.75 48.2 µM 1.50E-5 NEO1 69 8.70E-5
5 SGTC_2568 diosmetin 3.62 100.0 µM 2.83E-5 32 1.46E-4
6 SGTC_2806 7952793 3.57 58.4 µM 3.69E-5 36 1.81E-4
7 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 3.42 3.7 µM 7.08E-5 136 3.08E-4
8 SGTC_1044 1222-0021 3.29 1.8 µM 1.27E-4 heme biosynthesis & mitochondrial translocase 61 4.94E-4
9 SGTC_2425 5628681 3.24 7.8 µM 1.57E-4 58 5.89E-4
10 SGTC_1919 st067113 3.23 52.3 µM 1.65E-4 TSC3-RPN4 61 6.14E-4
11 SGTC_3242 9133940 3.23 49.5 µM 1.65E-4 30 6.15E-4
12 SGTC_1254 0550-0035 3.22 264.0 µM 1.76E-4 46 6.46E-4
13 SGTC_732 3970-1727 3.21 52.7 µM 1.78E-4 24 6.54E-4
14 SGTC_453 enantio-paf c16 3.20 10.0 µM 1.91E-4 220 6.93E-4
15 SGTC_757 1066-0008 3.16 322.0 µM 2.25E-4 Golgi 53 7.89E-4
16 SGTC_770 0389-1121 3.05 67.2 µM 3.55E-4 RPP1 & pyrimidine depletion 65 0.00115
17 SGTC_2678 equilin 3.03 100.0 µM 3.77E-4 57 0.00121
18 SGTC_1089 terbinafine 2.97 2.2 µM 4.94E-4 54 0.00151
19 SGTC_1006 4099-6547 2.96 255.0 µM 5.11E-4 82 0.00155
20 SGTC_725 1497-0963 2.91 19.0 µM 6.28E-4 copper-dependent oxidative stress 64 0.00183

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.179 1.06E-25 YER067C-A_d YER067C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W
0.170 3.41E-23 YGL017W ATE1 hom Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway
0.168 1.19E-22 YBR262C AIM5 hom Mitochondrial inner membrane protein; subunit of the mitochondrial inner membrane organizing system (MitOS, MICOS, or MINOS), a scaffold-like structure on the intermembrane space side of the inner membrane which has a role in the maintenance of crista junctions and inner membrane architecture
0.153 5.03E-19 YOR300W_d YOR300W_d hom Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7
0.148 6.95E-18 YHL006C SHU1 hom Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function
0.140 4.53E-16 YDL110C TMA17 hom Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion
0.140 4.63E-16 YBL086C_p YBL086C_p hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.139 6.48E-16 YDR146C SWI5 hom Transcription factor that recruits Mediator and Swi/Snf complexes; activates transcription of genes expressed at the M/G1 phase boundary and in G1 phase; required for expression of the HO gene controlling mating type switching; localization to nucleus occurs during G1 and appears to be regulated by phosphorylation by Cdc28p kinase; SWI5 has a paralog, ACE2, that arose from the whole genome duplication
0.138 9.03E-16 YML053C_p YML053C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and the nucleus; overexpression causes a cell cycle delay or arrest; YML053C is not an essential gene
0.134 5.45E-15 YDR046C BAP3 hom Amino acid permease; involved in uptake of cysteine, leucine, isoleucine and valine; BAP3 has a paralog, BAP2, that arose from the whole genome duplication
0.130 3.95E-14 YMR173W DDR48 hom DNA damage-responsive protein; expression is increased in response to heat-shock stress or treatments that produce DNA lesions; contains multiple repeats of the amino acid sequence NNNDSYGS; protein abundance increases in response to DNA replication stress
0.129 7.85E-14 YNL242W ATG2 hom Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress
0.126 2.52E-13 YHL017W_p YHL017W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein co-localizes with clathrin-coated vesicles; YHL017W has a paralog, PTM1, that arose from the whole genome duplication
0.124 4.85E-13 YGR251W NOP19 het Ribosome biogenesis factor; nucleolar protein associated with pre-rRNA components of the 90S preribosome, required for cleavage of pre-rRNA at A0, A1 and A2 sites; interacts with RNA helicase Dhr2p and RNA helicase-like protein Utp25p; required for incorporation of Utp25p into preribosomes
0.119 3.85E-12 YNL078W NIS1 hom Protein localized in the bud neck at G2/M phase; physically interacts with septins; possibly involved in a mitotic signaling network