YDR050C / TPI1

Triose phosphate isomerase, abundant glycolytic enzyme; mRNA half-life is regulated by iron availability; transcription is controlled by activators Reb1p, Gcr1p, and Rap1p through binding sites in the 5' non-coding region; inhibition of Tpi1p activity by PEP (phosphoenolpyruvate) stimulates redox metabolism in respiring cells; E104D mutation in human TPI causes a rare autosomal disease

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR050C / TPI1

Click on Significant Values for Screen Details ID:SGTC_46|Compound:0329-0187a|FD-Score:4.08|P-value:5.15E-6 ID:SGTC_100|Compound:0086-0128|FD-Score:-2.98|P-value:5.44E-4 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:-2.99|P-value:5.20E-4 ID:SGTC_191|Compound:3914-0123|FD-Score:2.90|P-value:9.04E-4 ID:SGTC_292|Compound:k038-0015|FD-Score:3.13|P-value:3.68E-4 ID:SGTC_505|Compound:gw-5074|FD-Score:3.43|P-value:1.06E-4 ID:SGTC_557|Compound:1315-0110|FD-Score:-3.14|P-value:2.87E-4 ID:SGTC_775|Compound:4577-0580|FD-Score:3.77|P-value:2.31E-5 ID:SGTC_870|Compound:0573-2213|FD-Score:-2.97|P-value:5.68E-4 ID:SGTC_891|Compound:0929-0073|FD-Score:3.10|P-value:4.17E-4 ID:SGTC_899|Compound:1083-0020|FD-Score:3.15|P-value:3.38E-4 ID:SGTC_927|Compound:2425-1185|FD-Score:3.08|P-value:4.47E-4 ID:SGTC_971|Compound:1315-0384|FD-Score:-2.92|P-value:6.72E-4 ID:SGTC_975|Compound:1335-0046|FD-Score:4.06|P-value:5.78E-6 ID:SGTC_987|Compound:1497-1013|FD-Score:3.06|P-value:4.81E-4 ID:SGTC_997|Compound:3996-0078|FD-Score:-3.29|P-value:1.54E-4 ID:SGTC_1145|Compound:3546-0597|FD-Score:3.34|P-value:1.61E-4 ID:SGTC_1274|Compound:0865-0114|FD-Score:3.13|P-value:3.69E-4 ID:SGTC_1581|Compound:himbacine|FD-Score:3.06|P-value:4.90E-4 ID:SGTC_1669|Compound:st015249|FD-Score:3.18|P-value:3.00E-4 ID:SGTC_2063|Compound:5246145|FD-Score:3.65|P-value:4.16E-5 ID:SGTC_2079|Compound:5212404|FD-Score:-2.99|P-value:5.21E-4 ID:SGTC_2083|Compound:5306046|FD-Score:-3.95|P-value:7.94E-6 ID:SGTC_2164|Compound:5685496|FD-Score:-3.43|P-value:8.68E-5 ID:SGTC_2309|Compound:7664088|FD-Score:3.99|P-value:8.04E-6 ID:SGTC_2310|Compound:7668677|FD-Score:-3.12|P-value:3.16E-4 ID:SGTC_2334|Compound:9017922|FD-Score:-3.37|P-value:1.12E-4 ID:SGTC_2409|Compound:alverine citrate|FD-Score:-2.83|P-value:9.39E-4 ID:SGTC_2428|Compound:5615643|FD-Score:-3.17|P-value:2.53E-4 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:3.56|P-value:6.19E-5 ID:SGTC_2618|Compound:avocadyne|FD-Score:2.99|P-value:6.29E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:-3.42|P-value:9.21E-5 ID:SGTC_2624|Compound:nerol|FD-Score:3.25|P-value:2.27E-4 ID:SGTC_2649|Compound:anisomycin|FD-Score:-2.96|P-value:5.84E-4 ID:SGTC_2932|Compound:9016711|FD-Score:4.17|P-value:3.33E-6 ID:SGTC_2968|Compound:9090663|FD-Score:7.04|P-value:1.19E-14 ID:SGTC_3204|Compound:9114183|FD-Score:3.85|P-value:1.60E-5 ID:SGTC_3284|Compound:9108171|FD-Score:3.55|P-value:6.48E-5 ID:SGTC_3295|Compound:9122362|FD-Score:-2.99|P-value:5.19E-4 ID:SGTC_3296|Compound:9122258|FD-Score:-3.71|P-value:2.45E-5 ID:SGTC_46|Compound:0329-0187a|FD-Score:4.08|P-value:5.15E-6 ID:SGTC_100|Compound:0086-0128|FD-Score:-2.98|P-value:5.44E-4 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:-2.99|P-value:5.20E-4 ID:SGTC_191|Compound:3914-0123|FD-Score:2.90|P-value:9.04E-4 ID:SGTC_292|Compound:k038-0015|FD-Score:3.13|P-value:3.68E-4 ID:SGTC_505|Compound:gw-5074|FD-Score:3.43|P-value:1.06E-4 ID:SGTC_557|Compound:1315-0110|FD-Score:-3.14|P-value:2.87E-4 ID:SGTC_775|Compound:4577-0580|FD-Score:3.77|P-value:2.31E-5 ID:SGTC_870|Compound:0573-2213|FD-Score:-2.97|P-value:5.68E-4 ID:SGTC_891|Compound:0929-0073|FD-Score:3.10|P-value:4.17E-4 ID:SGTC_899|Compound:1083-0020|FD-Score:3.15|P-value:3.38E-4 ID:SGTC_927|Compound:2425-1185|FD-Score:3.08|P-value:4.47E-4 ID:SGTC_971|Compound:1315-0384|FD-Score:-2.92|P-value:6.72E-4 ID:SGTC_975|Compound:1335-0046|FD-Score:4.06|P-value:5.78E-6 ID:SGTC_987|Compound:1497-1013|FD-Score:3.06|P-value:4.81E-4 ID:SGTC_997|Compound:3996-0078|FD-Score:-3.29|P-value:1.54E-4 ID:SGTC_1145|Compound:3546-0597|FD-Score:3.34|P-value:1.61E-4 ID:SGTC_1274|Compound:0865-0114|FD-Score:3.13|P-value:3.69E-4 ID:SGTC_1581|Compound:himbacine|FD-Score:3.06|P-value:4.90E-4 ID:SGTC_1669|Compound:st015249|FD-Score:3.18|P-value:3.00E-4 ID:SGTC_2063|Compound:5246145|FD-Score:3.65|P-value:4.16E-5 ID:SGTC_2079|Compound:5212404|FD-Score:-2.99|P-value:5.21E-4 ID:SGTC_2083|Compound:5306046|FD-Score:-3.95|P-value:7.94E-6 ID:SGTC_2164|Compound:5685496|FD-Score:-3.43|P-value:8.68E-5 ID:SGTC_2309|Compound:7664088|FD-Score:3.99|P-value:8.04E-6 ID:SGTC_2310|Compound:7668677|FD-Score:-3.12|P-value:3.16E-4 ID:SGTC_2334|Compound:9017922|FD-Score:-3.37|P-value:1.12E-4 ID:SGTC_2409|Compound:alverine citrate|FD-Score:-2.83|P-value:9.39E-4 ID:SGTC_2428|Compound:5615643|FD-Score:-3.17|P-value:2.53E-4 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:3.56|P-value:6.19E-5 ID:SGTC_2618|Compound:avocadyne|FD-Score:2.99|P-value:6.29E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:-3.42|P-value:9.21E-5 ID:SGTC_2624|Compound:nerol|FD-Score:3.25|P-value:2.27E-4 ID:SGTC_2649|Compound:anisomycin|FD-Score:-2.96|P-value:5.84E-4 ID:SGTC_2932|Compound:9016711|FD-Score:4.17|P-value:3.33E-6 ID:SGTC_2968|Compound:9090663|FD-Score:7.04|P-value:1.19E-14 ID:SGTC_3204|Compound:9114183|FD-Score:3.85|P-value:1.60E-5 ID:SGTC_3284|Compound:9108171|FD-Score:3.55|P-value:6.48E-5 ID:SGTC_3295|Compound:9122362|FD-Score:-2.99|P-value:5.19E-4 ID:SGTC_3296|Compound:9122258|FD-Score:-3.71|P-value:2.45E-5

Top fitness defect scores for YDR050C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2968 9090663 7.04 33.4 µM 1.19E-14 3 9.36E-13 1.09
2 SGTC_2932 9016711 4.17 44.5 µM 3.33E-6 7 1.53E-5 0.04
3 SGTC_46 0329-0187a 4.08 2.2 µM 5.15E-6 6 2.22E-5 0.18
4 SGTC_975 1335-0046 4.06 28.5 µM 5.78E-6 RPP1 & pyrimidine depletion 7 2.45E-5 0.03
5 SGTC_2309 7664088 3.99 163.9 µM 8.04E-6 11 3.25E-5 0.46
6 SGTC_3204 9114183 3.85 49.5 µM 1.60E-5 7 5.87E-5 0.09
7 SGTC_775 4577-0580 3.77 57.3 µM 2.31E-5 5 8.04E-5 0.00
8 SGTC_2063 5246145 3.65 200.0 µM 4.16E-5 8 1.33E-4 9.91E-4
9 SGTC_2579 2',5'-dihydroxy-4-methoxychalcone 3.56 13.2 µM 6.19E-5 11 1.88E-4 0.05
10 SGTC_3284 9108171 3.55 64.9 µM 6.48E-5 5 1.95E-4 0.12
11 SGTC_505 gw-5074 3.43 63.9 µM 1.06E-4 11 2.97E-4 0.04
12 SGTC_1145 3546-0597 3.34 10.2 µM 1.61E-4 mitochondrial processes 7 4.26E-4 0.10
13 SGTC_2624 nerol 3.25 100.0 µM 2.27E-4 9 5.74E-4 0.10
14 SGTC_1669 st015249 3.18 58.4 µM 3.00E-4 Golgi 5 7.30E-4 0.01
15 SGTC_899 1083-0020 3.15 478.0 µM 3.38E-4 8 8.08E-4 0.10
16 SGTC_292 k038-0015 3.13 474.0 µM 3.68E-4 7 8.71E-4 0.26
17 SGTC_1274 0865-0114 3.13 173.0 µM 3.69E-4 21 8.72E-4 0.01
18 SGTC_891 0929-0073 3.10 133.0 µM 4.17E-4 9 9.69E-4 0.02
19 SGTC_927 2425-1185 3.08 123.0 µM 4.47E-4 16 0.00103 0.03
20 SGTC_987 1497-1013 3.06 404.0 µM 4.81E-4 8 0.00110 0.03

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.121 1.70E-12 YDR392W SPT3 hom Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters
0.116 1.33E-11 YDR163W CWC15 hom Non-essential protein involved in pre-mRNA splicing, component of a complex containing Cef1p; has similarity to S. pombe Cwf15p
0.114 3.06E-11 YHR085W IPI1 het Component of the Rix1 complex (Rix1p-Ipi1p-Ipi3p) and possibly pre-replicative complexes (pre-RCs); required for processing of ITS2 sequences from 35S pre-rRNA; component of the pre-60S ribosomal particle with the dynein-related AAA-type ATPase Mdn1p; required for pre-RC formation and maintenance during DNA replication licensing; IPI1 is an essential gene
0.111 1.22E-10 YOR027W STI1 hom Hsp90 cochaperone, interacts with the Ssa group of the cytosolic Hsp70 chaperones and activates Ssa1p ATPase activity; interacts with Hsp90 chaperones and inhibits their ATPase activity; homolog of mammalian Hop
0.104 1.47E-9 YOR025W HST3 hom Member of the Sir2 family of NAD(+)-dependent protein deacetylases; involved along with Hst4p in telomeric silencing, cell cycle progression, radiation resistance, genomic stability and short-chain fatty acid metabolism
0.093 5.85E-8 YMR059W SEN15 het Subunit of the tRNA splicing endonuclease, which is composed of Sen2p, Sen15p, Sen34p, and Sen54p
0.090 1.90E-7 YNL263C YIF1 het Integral membrane protein required for the fusion of ER-derived COPII transport vesicles with the Golgi; interacts with Yip1p and Yos1p; localizes to the Golgi, the ER, and COPII vesicles; homolog of human YIPF3
0.088 3.08E-7 YNR034W SOL1 hom Protein with a possible role in tRNA export; shows similarity to 6-phosphogluconolactonase non-catalytic domains but does not exhibit this enzymatic activity; homologous to Sol3p and Sol4p; SOL1 has a paralog, SOL2, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.088 3.25E-7 YGR044C RME1 hom Zinc finger protein involved in control of meiosis; prevents meiosis by repressing IME1 expression and promotes mitosis by activating CLN2 expression; directly repressed by a1-alpha2 regulator; mediates cell type control of sporulation; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.086 5.91E-7 YDL109C_p YDL109C_p hom Putative lipase; involved in lipid metabolism; not an essential gene; YDL109C has a paralog, ROG1, that arose from the whole genome duplication
0.083 1.50E-6 YDR314C RAD34 hom Protein involved in nucleotide excision repair (NER); homologous to RAD4
0.082 2.02E-6 YNR063W_p YNR063W_p hom Putative zinc-cluster protein of unknown function
0.082 2.28E-6 YIL057C RGI2 hom Protein of unknown function involved in energy metabolism under respiratory conditions; expression induced under carbon limitation and repressed under high glucose
0.079 4.63E-6 YCR090C_p YCR090C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; YCR090C is not an essential gene
0.079 4.62E-6 YAL054C ACS1 hom Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions