YDR052C / DBF4

Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR052C / DBF4

Click on Significant Values for Screen Details ID:SGTC_72|Compound:0744-0168|FD-Score:-3.48|P-value:8.04E-4 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:-3.77|P-value:3.09E-4 ID:SGTC_110|Compound:0850-0284|FD-Score:-3.74|P-value:3.42E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:5.74|P-value:1.09E-7 ID:SGTC_287|Compound:k060-0039|FD-Score:4.15|P-value:9.14E-5 ID:SGTC_341|Compound:1486-1430|FD-Score:8.58|P-value:4.64E-15 ID:SGTC_514|Compound:4466-0038|FD-Score:4.34|P-value:4.51E-5 ID:SGTC_723|Compound:4182-0001|FD-Score:3.85|P-value:2.57E-4 ID:SGTC_882|Compound:0843-0131|FD-Score:-3.67|P-value:4.41E-4 ID:SGTC_907|Compound:3448-6986|FD-Score:4.14|P-value:9.35E-5 ID:SGTC_1065|Compound:prochlorperazine maleate|FD-Score:4.27|P-value:5.79E-5 ID:SGTC_1086|Compound:cerivastatin|FD-Score:-4.02|P-value:1.32E-4 ID:SGTC_1305|Compound:1181-0477|FD-Score:3.55|P-value:6.79E-4 ID:SGTC_1327|Compound:1436-0082|FD-Score:-4.00|P-value:1.43E-4 ID:SGTC_1500|Compound:4488-1866|FD-Score:3.58|P-value:6.16E-4 ID:SGTC_2401|Compound:5128683|FD-Score:-3.64|P-value:4.84E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.03|P-value:1.37E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:5.20|P-value:1.33E-6 ID:SGTC_2629|Compound:bisabolol|FD-Score:-3.42|P-value:9.53E-4 ID:SGTC_2680|Compound:butyl paraben|FD-Score:3.54|P-value:6.95E-4 ID:SGTC_2934|Compound:9036370|FD-Score:3.60|P-value:5.81E-4 ID:SGTC_3036|Compound:9090181|FD-Score:4.19|P-value:7.75E-5 ID:SGTC_72|Compound:0744-0168|FD-Score:-3.48|P-value:8.04E-4 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:-3.77|P-value:3.09E-4 ID:SGTC_110|Compound:0850-0284|FD-Score:-3.74|P-value:3.42E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:5.74|P-value:1.09E-7 ID:SGTC_287|Compound:k060-0039|FD-Score:4.15|P-value:9.14E-5 ID:SGTC_341|Compound:1486-1430|FD-Score:8.58|P-value:4.64E-15 ID:SGTC_514|Compound:4466-0038|FD-Score:4.34|P-value:4.51E-5 ID:SGTC_723|Compound:4182-0001|FD-Score:3.85|P-value:2.57E-4 ID:SGTC_882|Compound:0843-0131|FD-Score:-3.67|P-value:4.41E-4 ID:SGTC_907|Compound:3448-6986|FD-Score:4.14|P-value:9.35E-5 ID:SGTC_1065|Compound:prochlorperazine maleate|FD-Score:4.27|P-value:5.79E-5 ID:SGTC_1086|Compound:cerivastatin|FD-Score:-4.02|P-value:1.32E-4 ID:SGTC_1305|Compound:1181-0477|FD-Score:3.55|P-value:6.79E-4 ID:SGTC_1327|Compound:1436-0082|FD-Score:-4.00|P-value:1.43E-4 ID:SGTC_1500|Compound:4488-1866|FD-Score:3.58|P-value:6.16E-4 ID:SGTC_2401|Compound:5128683|FD-Score:-3.64|P-value:4.84E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.03|P-value:1.37E-4 ID:SGTC_2584|Compound:androsterone|FD-Score:5.20|P-value:1.33E-6 ID:SGTC_2629|Compound:bisabolol|FD-Score:-3.42|P-value:9.53E-4 ID:SGTC_2680|Compound:butyl paraben|FD-Score:3.54|P-value:6.95E-4 ID:SGTC_2934|Compound:9036370|FD-Score:3.60|P-value:5.81E-4 ID:SGTC_3036|Compound:9090181|FD-Score:4.19|P-value:7.75E-5

Top fitness defect scores for YDR052C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_341 1486-1430 8.58 230.0 µM 4.64E-15 5 4.91E-18 1.66
2 SGTC_268 camptothecin 5.74 39.6 µM 1.09E-7 DNA damage response 24 4.73E-9 0.11
3 SGTC_2584 androsterone 5.20 48.7 µM 1.33E-6 cell wall 17 9.92E-8 0.09
4 SGTC_514 4466-0038 4.34 2.6 nM 4.51E-5 cell wall 33 7.19E-6 0.14
5 SGTC_1065 prochlorperazine maleate 4.27 2.5 µM 5.79E-5 5 9.73E-6 0.23
6 SGTC_3036 9090181 4.19 49.5 µM 7.75E-5 3 1.38E-5 0.47
7 SGTC_287 k060-0039 4.15 29.6 µM 9.14E-5 8 1.69E-5 0.27
8 SGTC_907 3448-6986 4.14 75.5 µM 9.35E-5 5 1.74E-5 0.34
9 SGTC_2582 agaric acid 4.03 100.0 µM 1.37E-4 redox potentiating 32 2.76E-5 0.14
10 SGTC_723 4182-0001 3.85 115.0 µM 2.57E-4 5 5.92E-5 0.05
11 SGTC_2934 9036370 3.60 36.9 µM 5.81E-4 8 1.59E-4 0.22
12 SGTC_1500 4488-1866 3.58 171.0 µM 6.16E-4 RPP1 & pyrimidine depletion 3 1.71E-4 0.20
13 SGTC_1305 1181-0477 3.55 144.0 µM 6.79E-4 6 1.92E-4 0.03
14 SGTC_2680 butyl paraben 3.54 22.2 µM 6.95E-4 amide catabolism 1 1.97E-4 0.28
15 SGTC_128 0906-2981 3.37 13.4 µM 0.00117 17 3.70E-4 0.05
16 SGTC_814 3342-0569 3.33 96.0 µM 0.00134 8 4.36E-4 0.00
17 SGTC_1077 5-fluorocytosine 3.30 377.0 nM 0.00145 exosome 22 4.79E-4 0.05
18 SGTC_3350 9150499 3.29 3.6 µM 0.00150 cell wall 21 5.00E-4 0.01
19 SGTC_1455 4262-0120 3.25 131.0 µM 0.00170 plasma membrane duress 13 5.80E-4 0.13
20 SGTC_1334 1486-0610 3.24 236.0 µM 0.00173 60S ribosome export 9 5.94E-4 0.05

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.196 2.17E-30 YBR134W_d YBR134W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.192 2.56E-29 YNL106C INP52 hom Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain
0.191 4.63E-29 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.171 1.72E-23 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.159 1.58E-20 YLR102C APC9 hom Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
0.159 2.40E-20 YBL085W BOI1 hom Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication
0.156 1.02E-19 YPL210C SRP72 het Core component of the signal recognition particle (SRP) ribonucleoprotein (RNP) complex that functions in targeting nascent secretory proteins to the endoplasmic reticulum (ER) membrane
0.148 5.37E-18 YBR023C CHS3 hom Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan
0.144 5.65E-17 YDR121W DPB4 hom Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization
0.135 4.40E-15 YBR020W GAL1 hom Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication
0.133 1.04E-14 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.131 2.71E-14 YML103C NUP188 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188
0.130 4.88E-14 YGL033W HOP2 hom Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair
0.129 6.32E-14 YNL273W TOF1 hom Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion after DNA damage, facilitating gap repair of damaged DNA; interacts with the MCM helicase
0.128 1.02E-13 YDR435C PPM1 hom Carboxyl methyltransferase, methylates the C terminus of the protein phosphatase 2A catalytic subunit (Pph21p or Pph22p), which is important for complex formation with regulatory subunits