YDR059C / UBC5

Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived, abnormal, or excess proteins, including histone H3; central component of the cellular stress response; expression is heat inducible; protein abundance increases in response to DNA replication stress; UBC5 has a paralog, UBC4, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR059C / UBC5

Click on Significant Values for Screen Details ID:SGTC_437|Compound:s-farnesyl-l-cysteine methyl ester|FD-Score:-3.28|P-value:4.07E-4 ID:SGTC_827|Compound:3253-0474|FD-Score:3.39|P-value:2.97E-4 ID:SGTC_937|Compound:3122-0005|FD-Score:-3.15|P-value:6.54E-4 ID:SGTC_1034|Compound:k072-0165|FD-Score:3.58|P-value:1.42E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:3.25|P-value:4.88E-4 ID:SGTC_1214|Compound:0358-0031|FD-Score:3.68|P-value:9.53E-5 ID:SGTC_1262|Compound:0737-0114|FD-Score:4.45|P-value:3.12E-6 ID:SGTC_1330|Compound:1469-0054|FD-Score:3.15|P-value:7.00E-4 ID:SGTC_1372|Compound:2752-0194|FD-Score:-4.19|P-value:9.78E-6 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.17|P-value:1.08E-5 ID:SGTC_1835|Compound:st008666|FD-Score:-3.21|P-value:5.35E-4 ID:SGTC_1908|Compound:st060353|FD-Score:-3.23|P-value:4.94E-4 ID:SGTC_1975|Compound:st070967|FD-Score:4.88|P-value:3.69E-7 ID:SGTC_2002|Compound:4023503|FD-Score:3.83|P-value:5.12E-5 ID:SGTC_2304|Compound:7443016|FD-Score:3.23|P-value:5.36E-4 ID:SGTC_2306|Compound:7482397|FD-Score:6.02|P-value:5.02E-10 ID:SGTC_2311|Compound:7694451|FD-Score:-5.45|P-value:1.42E-8 ID:SGTC_2312|Compound:7706453|FD-Score:-4.90|P-value:2.95E-7 ID:SGTC_2317|Compound:5986382|FD-Score:3.68|P-value:9.37E-5 ID:SGTC_2328|Compound:6201408|FD-Score:3.13|P-value:7.42E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-3.24|P-value:4.80E-4 ID:SGTC_2568|Compound:diosmetin|FD-Score:3.46|P-value:2.28E-4 ID:SGTC_2662|Compound:daunorubicin|FD-Score:-3.06|P-value:8.91E-4 ID:SGTC_2942|Compound:9052777|FD-Score:4.05|P-value:1.98E-5 ID:SGTC_3129|Compound:9127257|FD-Score:-3.15|P-value:6.64E-4 ID:SGTC_3269|Compound:9137823|FD-Score:-3.13|P-value:6.97E-4 ID:SGTC_3305|Compound:9127027|FD-Score:-3.08|P-value:8.26E-4 ID:SGTC_437|Compound:s-farnesyl-l-cysteine methyl ester|FD-Score:-3.28|P-value:4.07E-4 ID:SGTC_827|Compound:3253-0474|FD-Score:3.39|P-value:2.97E-4 ID:SGTC_937|Compound:3122-0005|FD-Score:-3.15|P-value:6.54E-4 ID:SGTC_1034|Compound:k072-0165|FD-Score:3.58|P-value:1.42E-4 ID:SGTC_1204|Compound:4049-0315|FD-Score:3.25|P-value:4.88E-4 ID:SGTC_1214|Compound:0358-0031|FD-Score:3.68|P-value:9.53E-5 ID:SGTC_1262|Compound:0737-0114|FD-Score:4.45|P-value:3.12E-6 ID:SGTC_1330|Compound:1469-0054|FD-Score:3.15|P-value:7.00E-4 ID:SGTC_1372|Compound:2752-0194|FD-Score:-4.19|P-value:9.78E-6 ID:SGTC_1462|Compound:k081-0014|FD-Score:-4.17|P-value:1.08E-5 ID:SGTC_1835|Compound:st008666|FD-Score:-3.21|P-value:5.35E-4 ID:SGTC_1908|Compound:st060353|FD-Score:-3.23|P-value:4.94E-4 ID:SGTC_1975|Compound:st070967|FD-Score:4.88|P-value:3.69E-7 ID:SGTC_2002|Compound:4023503|FD-Score:3.83|P-value:5.12E-5 ID:SGTC_2304|Compound:7443016|FD-Score:3.23|P-value:5.36E-4 ID:SGTC_2306|Compound:7482397|FD-Score:6.02|P-value:5.02E-10 ID:SGTC_2311|Compound:7694451|FD-Score:-5.45|P-value:1.42E-8 ID:SGTC_2312|Compound:7706453|FD-Score:-4.90|P-value:2.95E-7 ID:SGTC_2317|Compound:5986382|FD-Score:3.68|P-value:9.37E-5 ID:SGTC_2328|Compound:6201408|FD-Score:3.13|P-value:7.42E-4 ID:SGTC_2400|Compound:5203494|FD-Score:-3.24|P-value:4.80E-4 ID:SGTC_2568|Compound:diosmetin|FD-Score:3.46|P-value:2.28E-4 ID:SGTC_2662|Compound:daunorubicin|FD-Score:-3.06|P-value:8.91E-4 ID:SGTC_2942|Compound:9052777|FD-Score:4.05|P-value:1.98E-5 ID:SGTC_3129|Compound:9127257|FD-Score:-3.15|P-value:6.64E-4 ID:SGTC_3269|Compound:9137823|FD-Score:-3.13|P-value:6.97E-4 ID:SGTC_3305|Compound:9127027|FD-Score:-3.08|P-value:8.26E-4

Top fitness defect scores for YDR059C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2306 7482397 6.02 179.5 µM 5.02E-10 RPP1 & pyrimidine depletion 2 8.93E-10
2 SGTC_1975 st070967 4.88 30.8 µM 3.69E-7 18 5.36E-7
3 SGTC_1262 0737-0114 4.45 49.6 µM 3.12E-6 12 4.25E-6
4 SGTC_2942 9052777 4.05 62.2 µM 1.98E-5 29 2.55E-5
5 SGTC_2002 4023503 3.83 131.0 µM 5.12E-5 mitochondrial processes 37 6.41E-5
6 SGTC_2317 5986382 3.68 200.0 µM 9.37E-5 19 1.15E-4
7 SGTC_1214 0358-0031 3.68 63.7 µM 9.53E-5 26 1.17E-4
8 SGTC_1034 k072-0165 3.58 12.7 µM 1.42E-4 Golgi 34 1.73E-4
9 SGTC_2568 diosmetin 3.46 100.0 µM 2.28E-4 37 2.73E-4
10 SGTC_827 3253-0474 3.39 693.0 µM 2.97E-4 40 3.53E-4
11 SGTC_1204 4049-0315 3.25 18.3 µM 4.88E-4 61 5.72E-4
12 SGTC_2304 7443016 3.23 91.4 µM 5.36E-4 43 6.28E-4
13 SGTC_1330 1469-0054 3.15 61.8 µM 7.00E-4 iron homeostasis 40 8.13E-4
14 SGTC_2328 6201408 3.13 200.0 µM 7.42E-4 45 8.61E-4
15 SGTC_453 enantio-paf c16 2.99 10.0 µM 0.00120 250 0.00137
16 SGTC_336 4111-0029 2.98 111.0 µM 0.00127 31 0.00145
17 SGTC_1206 3978-0017 2.96 132.0 µM 0.00133 ERG2 76 0.00152
18 SGTC_440 fluspirilene 2.93 35.1 µM 0.00151 67 0.00172
19 SGTC_3143 9086658 2.89 49.5 µM 0.00172 115 0.00195
20 SGTC_2372 9071633 2.87 200.0 µM 0.00180 55 0.00204

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.198 6.31E-31 YDR312W SSF2 hom Protein required for ribosomal large subunit maturation; functionally redundant with Ssf1p; member of the Brix family; SSF2 has a paralog, SSF1, that arose from the whole genome duplication
0.154 2.57E-19 YDR169C STB3 hom Ribosomal RNA processing element (RRPE)-binding protein; involved in the glucose-induced transition from quiescence to growth; restricted to nucleus in quiescent cells, released into cytoplasm after glucose repletion; binds Sin3p; relative distribution to the nucleus increases upon DNA replication stress
0.146 2.57E-17 YDR133C_d YDR133C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C
0.144 4.15E-17 YJR128W_d YJR128W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RSF2
0.136 2.55E-15 YDL176W YDL176W hom Protein of unknown function, predicted by computational methods to be involved in fructose-1,6-bisphosphatase (Fbp1p) degradation; interacts with components of the GID complex; YDL176W is not an essential gene
0.136 3.11E-15 YKR102W FLO10 hom Member of the FLO family of cell wall flocculation proteins; not expressed in most lab strains; overproduction induces flocculation that can be inhibited by mannose, sucrose, or glucose; overproduction also promotes haploid invasive growth and diploid filamentous growth
0.135 3.90E-15 YLL054C_p YLL054C_p hom Putative protein of unknown function with similarity to Pip2p, an oleate-specific transcriptional activator of peroxisome proliferation; YLL054C is not an essential gene
0.130 4.21E-14 YDR521W_d YDR521W_d hom Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol
0.127 1.31E-13 YJR146W_p YJR146W_p hom Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2
0.127 1.36E-13 YLR457C NBP1 het Spindle pole body (SPB) component; required for the insertion of the duplication plaque into the nuclear membrane during SPB duplication; essential for bipolar spindle formation; component of the Mps2p-Bbp1p complex; NBP1 has a paralog, YPR174C, that arose from the whole genome duplication
0.125 4.29E-13 YBR142W MAK5 het Essential nucleolar protein, putative DEAD-box RNA helicase required for maintenance of M1 dsRNA virus; involved in biogenesis of large (60S) ribosomal subunits
0.123 7.34E-13 YOL149W DCP1 het Subunit of the Dcp1p-Dcp2p decapping enzyme complex; decapping complex removes the 5' cap structure from mRNAs prior to their degradation; enhances the activity of catalytic subunit Dcp2p; regulated by DEAD box protein Dhh1p; forms cytoplasmic foci upon DNA replication stress
0.123 9.63E-13 YDR497C ITR1 hom Myo-inositol transporter; has strong similarity to the minor myo-inositol transporter Itr2p, member of the sugar transporter superfamily; expression is repressed by inositol and choline via Opi1p and derepressed via Ino2p and Ino4p; relative distribution to the vacuole increases upon DNA replication stress
0.122 1.20E-12 YMR137C PSO2 hom Nuclease required for DNA single- and double-strand break repair; acts at a post-incision step in repair of breaks that result from interstrand cross-links produced by a variety of mono- and bi-functional psoralen derivatives; induced by UV-irradiation; forms nuclear foci upon DNA replication stress
0.119 3.88E-12 YGL262W_p YGL262W_p hom Putative protein of unknown function; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein; YGL262W is not an essential gene