YDR060W / MAK21

Constituent of 66S pre-ribosomal particles, required for large (60S) ribosomal subunit biogenesis; involved in nuclear export of pre-ribosomes; required for maintenance of dsRNA virus; homolog of human CAATT-binding protein

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR060W / MAK21

Click on Significant Values for Screen Details ID:SGTC_9|Compound:0092-0002|FD-Score:4.48|P-value:8.48E-6 ID:SGTC_17|Compound:0960-0276|FD-Score:4.62|P-value:4.61E-6 ID:SGTC_66|Compound:0109-0187|FD-Score:-5.14|P-value:3.51E-7 ID:SGTC_92|Compound:0063-0024|FD-Score:3.43|P-value:5.00E-4 ID:SGTC_96|Compound:0929-0093|FD-Score:5.32|P-value:1.61E-7 ID:SGTC_143|Compound:4032-1194|FD-Score:3.62|P-value:2.60E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:4.03|P-value:5.55E-5 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:7.14|P-value:3.41E-12 ID:SGTC_468|Compound:gf-109203x|FD-Score:3.46|P-value:4.46E-4 ID:SGTC_469|Compound:w7|FD-Score:3.93|P-value:8.14E-5 ID:SGTC_470|Compound:aa-861|FD-Score:5.68|P-value:2.45E-8 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:7.30|P-value:1.15E-12 ID:SGTC_511|Compound:blebbistatin|FD-Score:3.23|P-value:9.61E-4 ID:SGTC_512|Compound:prima-1|FD-Score:5.17|P-value:3.37E-7 ID:SGTC_610|Compound:4215-0184|FD-Score:5.66|P-value:2.78E-8 ID:SGTC_633|Compound:0437-0093|FD-Score:6.64|P-value:8.92E-11 ID:SGTC_660|Compound:4031-1742|FD-Score:3.92|P-value:8.38E-5 ID:SGTC_679|Compound:0422-0091|FD-Score:3.43|P-value:4.97E-4 ID:SGTC_854|Compound:0422-0053|FD-Score:3.89|P-value:9.56E-5 ID:SGTC_867|Compound:0518-0118|FD-Score:3.70|P-value:1.92E-4 ID:SGTC_960|Compound:1222-0186|FD-Score:3.71|P-value:1.85E-4 ID:SGTC_1074|Compound:carmofur|FD-Score:7.17|P-value:2.72E-12 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:3.45|P-value:4.61E-4 ID:SGTC_1190|Compound:1486-1332|FD-Score:3.54|P-value:3.47E-4 ID:SGTC_1192|Compound:1488-0095|FD-Score:3.43|P-value:5.09E-4 ID:SGTC_1246|Compound:0422-0085|FD-Score:-3.27|P-value:8.05E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:3.29|P-value:7.97E-4 ID:SGTC_1388|Compound:3346-4487|FD-Score:-3.28|P-value:7.68E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:3.40|P-value:5.50E-4 ID:SGTC_1522|Compound:st012842|FD-Score:3.97|P-value:7.02E-5 ID:SGTC_1524|Compound:corynanthine|FD-Score:-5.63|P-value:2.78E-8 ID:SGTC_1562|Compound:morin hydrate|FD-Score:-3.35|P-value:6.12E-4 ID:SGTC_1574|Compound:spiramycin|FD-Score:4.42|P-value:1.11E-5 ID:SGTC_1587|Compound:estradiol valerate|FD-Score:-4.04|P-value:4.80E-5 ID:SGTC_1626|Compound:st003712|FD-Score:-5.07|P-value:4.95E-7 ID:SGTC_1887|Compound:st057648|FD-Score:3.42|P-value:5.19E-4 ID:SGTC_1966|Compound:st070145|FD-Score:4.24|P-value:2.33E-5 ID:SGTC_2241|Compound:6772625|FD-Score:3.42|P-value:5.26E-4 ID:SGTC_2292|Compound:7671323|FD-Score:3.23|P-value:9.79E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:-4.98|P-value:7.76E-7 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:-3.62|P-value:2.33E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:3.29|P-value:7.92E-4 ID:SGTC_2616|Compound:abietic acid|FD-Score:-3.38|P-value:5.52E-4 ID:SGTC_2791|Compound:5663188|FD-Score:3.33|P-value:7.02E-4 ID:SGTC_2847|Compound:9005661|FD-Score:-3.85|P-value:1.01E-4 ID:SGTC_2881|Compound:9058953|FD-Score:4.23|P-value:2.46E-5 ID:SGTC_2893|Compound:9048488|FD-Score:4.20|P-value:2.78E-5 ID:SGTC_3294|Compound:9122953|FD-Score:3.69|P-value:1.98E-4 ID:SGTC_9|Compound:0092-0002|FD-Score:4.48|P-value:8.48E-6 ID:SGTC_17|Compound:0960-0276|FD-Score:4.62|P-value:4.61E-6 ID:SGTC_66|Compound:0109-0187|FD-Score:-5.14|P-value:3.51E-7 ID:SGTC_92|Compound:0063-0024|FD-Score:3.43|P-value:5.00E-4 ID:SGTC_96|Compound:0929-0093|FD-Score:5.32|P-value:1.61E-7 ID:SGTC_143|Compound:4032-1194|FD-Score:3.62|P-value:2.60E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:4.03|P-value:5.55E-5 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:7.14|P-value:3.41E-12 ID:SGTC_468|Compound:gf-109203x|FD-Score:3.46|P-value:4.46E-4 ID:SGTC_469|Compound:w7|FD-Score:3.93|P-value:8.14E-5 ID:SGTC_470|Compound:aa-861|FD-Score:5.68|P-value:2.45E-8 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:7.30|P-value:1.15E-12 ID:SGTC_511|Compound:blebbistatin|FD-Score:3.23|P-value:9.61E-4 ID:SGTC_512|Compound:prima-1|FD-Score:5.17|P-value:3.37E-7 ID:SGTC_610|Compound:4215-0184|FD-Score:5.66|P-value:2.78E-8 ID:SGTC_633|Compound:0437-0093|FD-Score:6.64|P-value:8.92E-11 ID:SGTC_660|Compound:4031-1742|FD-Score:3.92|P-value:8.38E-5 ID:SGTC_679|Compound:0422-0091|FD-Score:3.43|P-value:4.97E-4 ID:SGTC_854|Compound:0422-0053|FD-Score:3.89|P-value:9.56E-5 ID:SGTC_867|Compound:0518-0118|FD-Score:3.70|P-value:1.92E-4 ID:SGTC_960|Compound:1222-0186|FD-Score:3.71|P-value:1.85E-4 ID:SGTC_1074|Compound:carmofur|FD-Score:7.17|P-value:2.72E-12 ID:SGTC_1077|Compound:5-fluorocytosine|FD-Score:3.45|P-value:4.61E-4 ID:SGTC_1190|Compound:1486-1332|FD-Score:3.54|P-value:3.47E-4 ID:SGTC_1192|Compound:1488-0095|FD-Score:3.43|P-value:5.09E-4 ID:SGTC_1246|Compound:0422-0085|FD-Score:-3.27|P-value:8.05E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:3.29|P-value:7.97E-4 ID:SGTC_1388|Compound:3346-4487|FD-Score:-3.28|P-value:7.68E-4 ID:SGTC_1459|Compound:k035-0031|FD-Score:3.40|P-value:5.50E-4 ID:SGTC_1522|Compound:st012842|FD-Score:3.97|P-value:7.02E-5 ID:SGTC_1524|Compound:corynanthine|FD-Score:-5.63|P-value:2.78E-8 ID:SGTC_1562|Compound:morin hydrate|FD-Score:-3.35|P-value:6.12E-4 ID:SGTC_1574|Compound:spiramycin|FD-Score:4.42|P-value:1.11E-5 ID:SGTC_1587|Compound:estradiol valerate|FD-Score:-4.04|P-value:4.80E-5 ID:SGTC_1626|Compound:st003712|FD-Score:-5.07|P-value:4.95E-7 ID:SGTC_1887|Compound:st057648|FD-Score:3.42|P-value:5.19E-4 ID:SGTC_1966|Compound:st070145|FD-Score:4.24|P-value:2.33E-5 ID:SGTC_2241|Compound:6772625|FD-Score:3.42|P-value:5.26E-4 ID:SGTC_2292|Compound:7671323|FD-Score:3.23|P-value:9.79E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:-4.98|P-value:7.76E-7 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:-3.62|P-value:2.33E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:3.29|P-value:7.92E-4 ID:SGTC_2616|Compound:abietic acid|FD-Score:-3.38|P-value:5.52E-4 ID:SGTC_2791|Compound:5663188|FD-Score:3.33|P-value:7.02E-4 ID:SGTC_2847|Compound:9005661|FD-Score:-3.85|P-value:1.01E-4 ID:SGTC_2881|Compound:9058953|FD-Score:4.23|P-value:2.46E-5 ID:SGTC_2893|Compound:9048488|FD-Score:4.20|P-value:2.78E-5 ID:SGTC_3294|Compound:9122953|FD-Score:3.69|P-value:1.98E-4

Top fitness defect scores for YDR060W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_471 1-acyl-paf 7.30 4.0 µM 1.15E-12 DNA intercalators 4 1.43E-13 0.04
2 SGTC_1074 carmofur 7.17 3.8 µM 2.72E-12 exosome 10 3.64E-13 0.07
3 SGTC_423 5-fluorouracil 7.14 457.0 µM 3.41E-12 exosome 10 4.65E-13 0.96
4 SGTC_633 0437-0093 6.64 97.7 µM 8.92E-11 DNA intercalators 3 1.58E-11 1.57
5 SGTC_470 aa-861 5.68 153.0 µM 2.45E-8 sphingolipid biosynthesis & PDR1 7 6.75E-9 0.61
6 SGTC_610 4215-0184 5.66 155.0 µM 2.78E-8 exosome 13 7.76E-9 0.20
7 SGTC_96 0929-0093 5.32 37.3 µM 1.61E-7 calcium & mitochondrial duress 1 5.16E-8 0.87
8 SGTC_512 prima-1 5.17 270.0 µM 3.37E-7 DNA intercalators 4 1.15E-7 0.08
9 SGTC_17 0960-0276 4.62 49.8 µM 4.61E-6 3 1.93E-6 0.19
10 SGTC_9 0092-0002 4.48 10.4 µM 8.48E-6 8 3.71E-6 0.10
11 SGTC_1574 spiramycin 4.42 23.7 µM 1.11E-5 3 4.97E-6 0.04
12 SGTC_1966 st070145 4.24 16.1 µM 2.33E-5 4 1.10E-5 0.09
13 SGTC_2881 9058953 4.23 52.0 µM 2.46E-5 2 1.17E-5 0.05
14 SGTC_2893 9048488 4.20 26.0 µM 2.78E-5 fatty acid desaturase (OLE1) 8 1.34E-5 0.19
15 SGTC_145 3737-0113 4.03 38.4 µM 5.55E-5 DNA intercalators 48 2.82E-5 0.05
16 SGTC_1522 st012842 3.97 45.6 µM 7.02E-5 RNA pol III & RNase P/MRP 13 3.63E-5 0.01
17 SGTC_469 w7 3.93 172.0 µM 8.14E-5 ERG2 4 4.25E-5 0.60
18 SGTC_660 4031-1742 3.92 102.0 µM 8.38E-5 redox potentiating 10 4.39E-5 0.01
19 SGTC_854 0422-0053 3.89 192.0 µM 9.56E-5 PDR1 6 5.06E-5 0.21
20 SGTC_960 1222-0186 3.71 7.7 µM 1.85E-4 10 1.03E-4 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.326 9.57E-84 YOR294W RRS1 het Essential protein that binds ribosomal protein L11; required for nuclear export of the 60S pre-ribosomal subunit during ribosome biogenesis; localizes to the nucleolus and in foci along nuclear periphery; cooperates with Ebp2p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering; mouse homolog shows altered expression in Huntington's disease model mice
0.321 3.49E-81 YDL058W USO1 het Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex
0.318 4.29E-79 YMR119W ASI1 hom Putative integral membrane E3 ubiquitin ligase; acts with Asi2p and Asi3p to ensure the fidelity of SPS-sensor signalling by maintaining the dormant repressed state of gene expression in the absence of inducing signals; ASI1 has a paralog, ASI3, that arose from the whole genome duplication
0.283 9.17E-63 YGR156W PTI1 het Essential protein that is a component of CPF (cleavage and polyadenylation factor); involved in 3' end formation of snoRNA and mRNA; interacts directly with Pta1p; has similarity to mammalian Cleavage-Stimulation Factor CstF-64
0.271 1.35E-57 YER003C PMI40 het Mannose-6-phosphate isomerase, catalyzes the interconversion of fructose-6-P and mannose-6-P; required for early steps in protein mannosylation
0.268 2.79E-56 YMR208W ERG12 het Mevalonate kinase, acts in the biosynthesis of isoprenoids and sterols, including ergosterol, from mevalonate
0.246 2.04E-47 YDR458C HEH2 hom Inner nuclear membrane (INM) protein; contains helix-extension-helix (HEH) motif, nuclear localization signal sequence; targeting to the INM requires the Srp1p-Kap95p karyopherins and the Ran cycle
0.245 5.23E-47 YGR195W SKI6 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp41p (EXOSC4)
0.231 4.91E-42 YOR278W HEM4 het Uroporphyrinogen III synthase, catalyzes the conversion of hydroxymethylbilane to uroporphyrinogen III, the fourth step in heme biosynthesis; deficiency in the human homolog can result in the disease congenital erythropoietic porphyria
0.225 1.53E-39 YGL200C EMP24 hom Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles
0.222 1.15E-38 YNL317W PFS2 het Integral subunit of the pre-mRNA cleavage and polyadenylation factor (CPF) complex; plays an essential role in mRNA 3'-end formation by bridging different processing factors and thereby promoting the assembly of the processing complex
0.212 1.91E-35 YPL044C_d YPL044C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP4/YPL043W
0.206 1.77E-33 YKL172W EBP2 het Required for 25S rRNA maturation and 60S ribosomal subunit assembly; localizes to the nucleolus and in foci along nuclear periphery; constituent of 66S pre-ribosomal particles; cooperates with Rrs1p and Mps3p to mediate telomere clustering by binding Sir4p, but is not involved in telomere tethering
0.204 7.38E-33 YDR362C TFC6 het One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110
0.202 3.93E-32 YDL111C RRP42 het Exosome non-catalytic core component; involved in 3'-5' RNA processing and degradation in both the nucleus and the cytoplasm; has similarity to E. coli RNase PH and to human hRrp42p (EXOSC7)