YDR068W / DOS2

Protein of unknown function, green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR068W / DOS2

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:3.96|P-value:3.67E-6 ID:SGTC_152|Compound:4469-0003|FD-Score:3.39|P-value:6.08E-5 ID:SGTC_354|Compound:2783-3500|FD-Score:2.83|P-value:6.85E-4 ID:SGTC_362|Compound:0310-0100|FD-Score:3.44|P-value:4.86E-5 ID:SGTC_508|Compound:roscovitine|FD-Score:2.83|P-value:6.68E-4 ID:SGTC_509|Compound:pifithrin|FD-Score:2.95|P-value:4.11E-4 ID:SGTC_510|Compound:ag-490|FD-Score:3.34|P-value:7.61E-5 ID:SGTC_672|Compound:1441-0018|FD-Score:-3.40|P-value:5.90E-5 ID:SGTC_760|Compound:0568-0725|FD-Score:3.16|P-value:1.70E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:-2.88|P-value:5.44E-4 ID:SGTC_949|Compound:1165-0505|FD-Score:3.03|P-value:2.97E-4 ID:SGTC_978|Compound:1416-0512|FD-Score:3.08|P-value:2.46E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.31|P-value:8.79E-5 ID:SGTC_1208|Compound:2323-2347|FD-Score:3.53|P-value:3.22E-5 ID:SGTC_1372|Compound:2752-0194|FD-Score:-3.02|P-value:3.19E-4 ID:SGTC_1563|Compound:conessine|FD-Score:4.54|P-value:1.38E-7 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:3.37|P-value:6.74E-5 ID:SGTC_1875|Compound:nocodazole|FD-Score:2.81|P-value:7.21E-4 ID:SGTC_1946|Compound:st076057|FD-Score:3.38|P-value:6.44E-5 ID:SGTC_1947|Compound:st076332|FD-Score:-2.99|P-value:3.55E-4 ID:SGTC_2020|Compound:4017018|FD-Score:2.85|P-value:6.11E-4 ID:SGTC_2034|Compound:5197015|FD-Score:2.80|P-value:7.63E-4 ID:SGTC_2048|Compound:5246248|FD-Score:3.25|P-value:1.17E-4 ID:SGTC_2099|Compound:5377090|FD-Score:3.80|P-value:8.33E-6 ID:SGTC_2127|Compound:5253358|FD-Score:-2.87|P-value:5.80E-4 ID:SGTC_2269|Compound:7935389|FD-Score:-3.01|P-value:3.21E-4 ID:SGTC_2372|Compound:9071633|FD-Score:2.75|P-value:9.26E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:2.96|P-value:4.05E-4 ID:SGTC_2476|Compound:5806916|FD-Score:-3.53|P-value:3.16E-5 ID:SGTC_2482|Compound:5981709|FD-Score:3.02|P-value:3.09E-4 ID:SGTC_2487|Compound:5918373|FD-Score:3.00|P-value:3.42E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.23|P-value:1.24E-4 ID:SGTC_2664|Compound:lyso-paf C-16|FD-Score:-3.10|P-value:2.20E-4 ID:SGTC_2714|Compound:st079313|FD-Score:3.52|P-value:3.37E-5 ID:SGTC_2862|Compound:9027634|FD-Score:-3.41|P-value:5.68E-5 ID:SGTC_2871|Compound:9038510|FD-Score:4.99|P-value:7.97E-9 ID:SGTC_2876|Compound:9059903|FD-Score:3.06|P-value:2.63E-4 ID:SGTC_2972|Compound:9093619|FD-Score:-4.28|P-value:6.20E-7 ID:SGTC_3158|Compound:9100007|FD-Score:-4.22|P-value:8.87E-7 ID:SGTC_6|Compound:0844-0013|FD-Score:3.96|P-value:3.67E-6 ID:SGTC_152|Compound:4469-0003|FD-Score:3.39|P-value:6.08E-5 ID:SGTC_354|Compound:2783-3500|FD-Score:2.83|P-value:6.85E-4 ID:SGTC_362|Compound:0310-0100|FD-Score:3.44|P-value:4.86E-5 ID:SGTC_508|Compound:roscovitine|FD-Score:2.83|P-value:6.68E-4 ID:SGTC_509|Compound:pifithrin|FD-Score:2.95|P-value:4.11E-4 ID:SGTC_510|Compound:ag-490|FD-Score:3.34|P-value:7.61E-5 ID:SGTC_672|Compound:1441-0018|FD-Score:-3.40|P-value:5.90E-5 ID:SGTC_760|Compound:0568-0725|FD-Score:3.16|P-value:1.70E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:-2.88|P-value:5.44E-4 ID:SGTC_949|Compound:1165-0505|FD-Score:3.03|P-value:2.97E-4 ID:SGTC_978|Compound:1416-0512|FD-Score:3.08|P-value:2.46E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.31|P-value:8.79E-5 ID:SGTC_1208|Compound:2323-2347|FD-Score:3.53|P-value:3.22E-5 ID:SGTC_1372|Compound:2752-0194|FD-Score:-3.02|P-value:3.19E-4 ID:SGTC_1563|Compound:conessine|FD-Score:4.54|P-value:1.38E-7 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:3.37|P-value:6.74E-5 ID:SGTC_1875|Compound:nocodazole|FD-Score:2.81|P-value:7.21E-4 ID:SGTC_1946|Compound:st076057|FD-Score:3.38|P-value:6.44E-5 ID:SGTC_1947|Compound:st076332|FD-Score:-2.99|P-value:3.55E-4 ID:SGTC_2020|Compound:4017018|FD-Score:2.85|P-value:6.11E-4 ID:SGTC_2034|Compound:5197015|FD-Score:2.80|P-value:7.63E-4 ID:SGTC_2048|Compound:5246248|FD-Score:3.25|P-value:1.17E-4 ID:SGTC_2099|Compound:5377090|FD-Score:3.80|P-value:8.33E-6 ID:SGTC_2127|Compound:5253358|FD-Score:-2.87|P-value:5.80E-4 ID:SGTC_2269|Compound:7935389|FD-Score:-3.01|P-value:3.21E-4 ID:SGTC_2372|Compound:9071633|FD-Score:2.75|P-value:9.26E-4 ID:SGTC_2419|Compound:paclitaxel|FD-Score:2.96|P-value:4.05E-4 ID:SGTC_2476|Compound:5806916|FD-Score:-3.53|P-value:3.16E-5 ID:SGTC_2482|Compound:5981709|FD-Score:3.02|P-value:3.09E-4 ID:SGTC_2487|Compound:5918373|FD-Score:3.00|P-value:3.42E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.23|P-value:1.24E-4 ID:SGTC_2664|Compound:lyso-paf C-16|FD-Score:-3.10|P-value:2.20E-4 ID:SGTC_2714|Compound:st079313|FD-Score:3.52|P-value:3.37E-5 ID:SGTC_2862|Compound:9027634|FD-Score:-3.41|P-value:5.68E-5 ID:SGTC_2871|Compound:9038510|FD-Score:4.99|P-value:7.97E-9 ID:SGTC_2876|Compound:9059903|FD-Score:3.06|P-value:2.63E-4 ID:SGTC_2972|Compound:9093619|FD-Score:-4.28|P-value:6.20E-7 ID:SGTC_3158|Compound:9100007|FD-Score:-4.22|P-value:8.87E-7

Top fitness defect scores for YDR068W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2871 9038510 4.99 13.0 µM 7.97E-9 6 3.04E-7
2 SGTC_1563 conessine 4.54 56.1 µM 1.38E-7 7 2.86E-6
3 SGTC_6 0844-0013 3.96 19.8 µM 3.67E-6 88 3.78E-5
4 SGTC_2099 5377090 3.80 177.4 µM 8.33E-6 27 7.21E-5
5 SGTC_1208 2323-2347 3.53 50.3 µM 3.22E-5 13 2.10E-4
6 SGTC_2714 st079313 3.52 56.9 µM 3.37E-5 14 2.17E-4
7 SGTC_362 0310-0100 3.44 9.0 µM 4.86E-5 RPP1 & pyrimidine depletion 55 2.90E-4
8 SGTC_152 4469-0003 3.39 551.2 µM 6.08E-5 39 3.46E-4
9 SGTC_1946 st076057 3.38 65.1 µM 6.44E-5 15 3.63E-4
10 SGTC_1572 epiandrosterone 3.37 68.9 µM 6.74E-5 45 3.76E-4
11 SGTC_510 ag-490 3.34 170.0 µM 7.61E-5 9 4.14E-4
12 SGTC_2048 5246248 3.25 200.0 µM 1.17E-4 18 5.80E-4
13 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 3.23 3.7 µM 1.24E-4 161 6.09E-4
14 SGTC_760 0568-0725 3.16 82.9 µM 1.70E-4 32 7.80E-4
15 SGTC_978 1416-0512 3.08 87.4 µM 2.46E-4 redox potentiating 63 0.00105
16 SGTC_2876 9059903 3.06 26.0 µM 2.63E-4 42 0.00111
17 SGTC_949 1165-0505 3.03 146.0 µM 2.97E-4 70 0.00122
18 SGTC_2482 5981709 3.02 142.7 µM 3.09E-4 PDR1 92 0.00126
19 SGTC_2487 5918373 3.00 44.0 µM 3.42E-4 56 0.00136
20 SGTC_2419 paclitaxel 2.96 166.5 µM 4.05E-4 112 0.00156

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.269 1.46E-56 YGL017W ATE1 hom Arginyl-tRNA-protein transferase, catalyzes post-translational conjugation of arginine to the amino termini of acceptor proteins which are then subject to degradation via the N-end rule pathway
0.205 3.11E-33 YGR236C SPG1 hom Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.197 8.23E-31 YKR009C FOX2 hom Multifunctional enzyme of the peroxisomal fatty acid beta-oxidation pathway; has 3-hydroxyacyl-CoA dehydrogenase and enoyl-CoA hydratase activities
0.171 1.83E-23 YER067C-A_d YER067C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YER067W
0.162 3.91E-21 YDL049C KNH1 hom Protein with similarity to Kre9p, which is involved in cell wall beta 1,6-glucan synthesis; overproduction suppresses growth defects of a kre9 null mutant; required for propionic acid resistance
0.160 1.04E-20 YLR288C MEC3 hom DNA damage and meiotic pachytene checkpoint protein; subunit of a heterotrimeric complex (Rad17p-Mec3p-Ddc1p) that forms a sliding clamp, loaded onto partial duplex DNA by a clamp loader complex; homolog of human and S. pombe Hus1
0.156 1.17E-19 YDR340W_d YDR340W_d hom Putative protein of unknown function
0.154 2.50E-19 YGL037C PNC1 hom Nicotinamidase that converts nicotinamide to nicotinic acid; part of the NAD(+) salvage pathway; required for life span extension by calorie restriction; PNC1 expression responds to all known stimuli that extend replicative life span; protein increases in abundance and relative distribution to cytoplasmic foci decreases upon DNA replication stress
0.152 7.28E-19 YNL242W ATG2 hom Peripheral membrane protein required for autophagic vesicle formation; also required for vesicle formation during pexophagy and the cytoplasm-to-vaucole targeting (Cvt) pathway; involved in Atg9p cycling between the phagophore assembly site and mitochondria; forms cytoplasmic foci upon DNA replication stress
0.150 2.73E-18 YNL176C_p TDA7_p hom Cell cycle-regulated gene of unknown function, promoter bound by Fkh2p; null mutant is sensitive to expression of the top1-T722A allele
0.150 2.96E-18 YOR300W_d YOR300W_d hom Dubious open reading frame, unlikely to encode a protein; overlaps with verified gene BUD7/YOR299W; mutation affects bipolar budding and bud site selection, though phenotype could be due to the mutation's effects on BUD7
0.145 2.60E-17 YDR306C_p YDR306C_p hom F-box protein of unknown function; interacts with Sgt1p via a Leucine-Rich Repeat (LRR) domain
0.143 9.76E-17 YDR089W YDR089W hom Protein of unknown function; deletion confers resistance to nickel; contains an SPX domain, which is found in proteins involved in phosphate homeostasis; relocalizes from vacuole to cytoplasm upon DNA replication stress
0.140 3.41E-16 YMR173W DDR48 hom DNA damage-responsive protein; expression is increased in response to heat-shock stress or treatments that produce DNA lesions; contains multiple repeats of the amino acid sequence NNNDSYGS; protein abundance increases in response to DNA replication stress
0.136 3.10E-15 YDR051C DET1 hom Acid phosphatase involved in the non-vesicular transport of sterols in both directions between the endoplasmic reticulum and plasma membrane; deletion confers sensitivity to nickel