YDR078C / SHU2

Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR078C / SHU2

Click on Significant Values for Screen Details ID:SGTC_17|Compound:0960-0276|FD-Score:3.22|P-value:5.66E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:3.07|P-value:9.76E-4 ID:SGTC_244|Compound:nsc-207895|FD-Score:4.11|P-value:1.65E-5 ID:SGTC_246|Compound:cycloheximide|FD-Score:4.07|P-value:1.93E-5 ID:SGTC_335|Compound:4077-0256|FD-Score:-4.55|P-value:1.83E-6 ID:SGTC_420|Compound:mitomycin C|FD-Score:3.53|P-value:1.79E-4 ID:SGTC_604|Compound:1000-0114|FD-Score:-4.24|P-value:7.81E-6 ID:SGTC_605|Compound:1315-0399|FD-Score:3.76|P-value:7.24E-5 ID:SGTC_608|Compound:k048-0125|FD-Score:4.37|P-value:5.04E-6 ID:SGTC_653|Compound:0203-0016|FD-Score:3.30|P-value:4.31E-4 ID:SGTC_671|Compound:0560-0003|FD-Score:-4.28|P-value:6.56E-6 ID:SGTC_740|Compound:k014-0057|FD-Score:3.14|P-value:7.65E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:4.79|P-value:6.24E-7 ID:SGTC_1128|Compound:3448-9350|FD-Score:3.56|P-value:1.61E-4 ID:SGTC_1194|Compound:1488-0194|FD-Score:5.16|P-value:8.99E-8 ID:SGTC_1202|Compound:1988-0068|FD-Score:3.39|P-value:3.13E-4 ID:SGTC_1409|Compound:3909-8004|FD-Score:-3.93|P-value:3.19E-5 ID:SGTC_1435|Compound:4092-0789|FD-Score:6.46|P-value:3.20E-11 ID:SGTC_1715|Compound:st032160|FD-Score:4.62|P-value:1.51E-6 ID:SGTC_1732|Compound:st034308|FD-Score:-3.59|P-value:1.29E-4 ID:SGTC_1734|Compound:st037168|FD-Score:-3.76|P-value:6.49E-5 ID:SGTC_1761|Compound:st044296|FD-Score:3.54|P-value:1.77E-4 ID:SGTC_1936|Compound:st074407|FD-Score:4.32|P-value:6.36E-6 ID:SGTC_1980|Compound:st071811|FD-Score:3.57|P-value:1.55E-4 ID:SGTC_2099|Compound:5377090|FD-Score:4.18|P-value:1.17E-5 ID:SGTC_2452|Compound:5863136|FD-Score:-3.61|P-value:1.19E-4 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:-3.88|P-value:3.85E-5 ID:SGTC_2537|Compound:larixol|FD-Score:3.06|P-value:9.96E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:-4.40|P-value:3.78E-6 ID:SGTC_2666|Compound:tannic acid|FD-Score:-3.29|P-value:3.97E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:-3.38|P-value:2.85E-4 ID:SGTC_2755|Compound:lomerizine|FD-Score:3.32|P-value:4.05E-4 ID:SGTC_2757|Compound:clomiphene|FD-Score:4.50|P-value:2.63E-6 ID:SGTC_2835|Compound:9006588|FD-Score:-3.42|P-value:2.48E-4 ID:SGTC_2845|Compound:9003024|FD-Score:-3.11|P-value:7.60E-4 ID:SGTC_2868|Compound:9035608|FD-Score:3.45|P-value:2.50E-4 ID:SGTC_2877|Compound:9040724|FD-Score:3.52|P-value:1.90E-4 ID:SGTC_2916|Compound:7875993|FD-Score:-3.51|P-value:1.72E-4 ID:SGTC_2917|Compound:7979103|FD-Score:3.18|P-value:6.73E-4 ID:SGTC_2925|Compound:9010536|FD-Score:4.13|P-value:1.51E-5 ID:SGTC_3106|Compound:9121286|FD-Score:-3.99|P-value:2.45E-5 ID:SGTC_3121|Compound:9125696|FD-Score:3.54|P-value:1.74E-4 ID:SGTC_3165|Compound:9101544|FD-Score:3.15|P-value:7.36E-4 ID:SGTC_3247|Compound:9135149|FD-Score:5.42|P-value:2.07E-8 ID:SGTC_3257|Compound:9139206|FD-Score:3.83|P-value:5.34E-5 ID:SGTC_17|Compound:0960-0276|FD-Score:3.22|P-value:5.66E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:3.07|P-value:9.76E-4 ID:SGTC_244|Compound:nsc-207895|FD-Score:4.11|P-value:1.65E-5 ID:SGTC_246|Compound:cycloheximide|FD-Score:4.07|P-value:1.93E-5 ID:SGTC_335|Compound:4077-0256|FD-Score:-4.55|P-value:1.83E-6 ID:SGTC_420|Compound:mitomycin C|FD-Score:3.53|P-value:1.79E-4 ID:SGTC_604|Compound:1000-0114|FD-Score:-4.24|P-value:7.81E-6 ID:SGTC_605|Compound:1315-0399|FD-Score:3.76|P-value:7.24E-5 ID:SGTC_608|Compound:k048-0125|FD-Score:4.37|P-value:5.04E-6 ID:SGTC_653|Compound:0203-0016|FD-Score:3.30|P-value:4.31E-4 ID:SGTC_671|Compound:0560-0003|FD-Score:-4.28|P-value:6.56E-6 ID:SGTC_740|Compound:k014-0057|FD-Score:3.14|P-value:7.65E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:4.79|P-value:6.24E-7 ID:SGTC_1128|Compound:3448-9350|FD-Score:3.56|P-value:1.61E-4 ID:SGTC_1194|Compound:1488-0194|FD-Score:5.16|P-value:8.99E-8 ID:SGTC_1202|Compound:1988-0068|FD-Score:3.39|P-value:3.13E-4 ID:SGTC_1409|Compound:3909-8004|FD-Score:-3.93|P-value:3.19E-5 ID:SGTC_1435|Compound:4092-0789|FD-Score:6.46|P-value:3.20E-11 ID:SGTC_1715|Compound:st032160|FD-Score:4.62|P-value:1.51E-6 ID:SGTC_1732|Compound:st034308|FD-Score:-3.59|P-value:1.29E-4 ID:SGTC_1734|Compound:st037168|FD-Score:-3.76|P-value:6.49E-5 ID:SGTC_1761|Compound:st044296|FD-Score:3.54|P-value:1.77E-4 ID:SGTC_1936|Compound:st074407|FD-Score:4.32|P-value:6.36E-6 ID:SGTC_1980|Compound:st071811|FD-Score:3.57|P-value:1.55E-4 ID:SGTC_2099|Compound:5377090|FD-Score:4.18|P-value:1.17E-5 ID:SGTC_2452|Compound:5863136|FD-Score:-3.61|P-value:1.19E-4 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:-3.88|P-value:3.85E-5 ID:SGTC_2537|Compound:larixol|FD-Score:3.06|P-value:9.96E-4 ID:SGTC_2544|Compound:sphondin|FD-Score:-4.40|P-value:3.78E-6 ID:SGTC_2666|Compound:tannic acid|FD-Score:-3.29|P-value:3.97E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:-3.38|P-value:2.85E-4 ID:SGTC_2755|Compound:lomerizine|FD-Score:3.32|P-value:4.05E-4 ID:SGTC_2757|Compound:clomiphene|FD-Score:4.50|P-value:2.63E-6 ID:SGTC_2835|Compound:9006588|FD-Score:-3.42|P-value:2.48E-4 ID:SGTC_2845|Compound:9003024|FD-Score:-3.11|P-value:7.60E-4 ID:SGTC_2868|Compound:9035608|FD-Score:3.45|P-value:2.50E-4 ID:SGTC_2877|Compound:9040724|FD-Score:3.52|P-value:1.90E-4 ID:SGTC_2916|Compound:7875993|FD-Score:-3.51|P-value:1.72E-4 ID:SGTC_2917|Compound:7979103|FD-Score:3.18|P-value:6.73E-4 ID:SGTC_2925|Compound:9010536|FD-Score:4.13|P-value:1.51E-5 ID:SGTC_3106|Compound:9121286|FD-Score:-3.99|P-value:2.45E-5 ID:SGTC_3121|Compound:9125696|FD-Score:3.54|P-value:1.74E-4 ID:SGTC_3165|Compound:9101544|FD-Score:3.15|P-value:7.36E-4 ID:SGTC_3247|Compound:9135149|FD-Score:5.42|P-value:2.07E-8 ID:SGTC_3257|Compound:9139206|FD-Score:3.83|P-value:5.34E-5

Top fitness defect scores for YDR078C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1435 4092-0789 6.46 56.7 µM 3.20E-11 plasma membrane duress 6 5.36E-11
2 SGTC_3247 9135149 5.42 49.5 µM 2.07E-8 DNA damage response 15 2.95E-8
3 SGTC_1194 1488-0194 5.16 225.0 µM 8.99E-8 13 1.23E-7
4 SGTC_915 methyl methanesulfonate (MMS) 4.79 110.0 mM 6.24E-7 DNA damage response 19 8.15E-7
5 SGTC_1715 st032160 4.62 10.2 µM 1.51E-6 29 1.94E-6
6 SGTC_2757 clomiphene 4.50 4.2 µM 2.63E-6 fatty acid desaturase (OLE1) 26 3.33E-6
7 SGTC_608 k048-0125 4.37 159.0 µM 5.04E-6 21 6.27E-6
8 SGTC_1936 st074407 4.32 74.3 µM 6.36E-6 DNA damage response 23 7.86E-6
9 SGTC_2099 5377090 4.18 177.4 µM 1.17E-5 16 1.43E-5
10 SGTC_2925 9010536 4.13 32.5 µM 1.51E-5 azole & statin 38 1.82E-5
11 SGTC_244 nsc-207895 4.11 1.3 µM 1.65E-5 DNA damage response 17 2.00E-5
12 SGTC_246 cycloheximide 4.07 667.0 µM 1.93E-5 12 2.32E-5
13 SGTC_3257 9139206 3.83 33.0 µM 5.34E-5 4 6.28E-5
14 SGTC_605 1315-0399 3.76 119.0 µM 7.24E-5 Golgi 30 8.45E-5
15 SGTC_1980 st071811 3.57 45.7 µM 1.55E-4 RPP1 & pyrimidine depletion 29 1.78E-4
16 SGTC_1128 3448-9350 3.56 235.0 µM 1.61E-4 33 1.85E-4
17 SGTC_3121 9125696 3.54 49.5 µM 1.74E-4 7 1.99E-4
18 SGTC_1761 st044296 3.54 37.0 µM 1.77E-4 21 2.03E-4
19 SGTC_420 mitomycin C 3.53 81.9 mM 1.79E-4 25 2.04E-4
20 SGTC_2877 9040724 3.52 71.4 µM 1.90E-4 21 2.17E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.115 2.12E-11 YGL087C MMS2 hom Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress
0.111 1.05E-10 YGL163C RAD54 hom DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress
0.102 3.36E-9 YLR032W RAD5 hom DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress
0.099 9.64E-9 YMR280C CAT8 hom Zinc cluster transcriptional activator; necessary for derepression of a variety of genes under non-fermentative growth conditions, active after diauxic shift, binds carbon source responsive elements; relative distribution to the nucleus increases upon DNA replication stress
0.096 2.86E-8 YDR004W RAD57 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
0.096 3.02E-8 YDR222W_p YDR222W_p hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
0.095 3.40E-8 YDR076W RAD55 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
0.088 3.99E-7 YOR120W GCY1 hom Glycerol dehydrogenase;involved in an alternative pathway for glycerol catabolism used under microaerobic conditions; also has mRNA binding activity; member of the aldo-keto reductase (AKR) family; protein abundance increases in response to DNA replication stress; GCY1 has a paralog, YPR1, that arose from the whole genome duplication
0.087 4.07E-7 YDR313C PIB1 hom RING-type ubiquitin ligase of the endosomal and vacuolar membranes, binds phosphatidylinositol(3)-phosphate; contains a FYVE finger domain
0.087 4.86E-7 YCR066W RAD18 hom E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA
0.087 4.99E-7 YBR098W MMS4 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.086 5.85E-7 YGL092W NUP145 het Essential protein with distinct roles in two nuclear pore subcomplexes; catalyzes its own proteolytic cleavage in vivo to generate a C-terminal fragment that is a structural component of the Nup84p subcomplex (with roles in NPC biogenesis and localization of genes to the nuclear periphery), and an N-terminal fragment that is one of several FG-nucleoporins within the NPC central core directly responsible for nucleocytoplasmic transport; homologous to human NUP98
0.085 7.58E-7 YIR016W_p YIR016W_p hom Putative protein of unknown function; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; overexpression causes a cell cycle delay or arrest; non-essential gene; YIR016W has a paralog, YOL036W, that arose from the whole genome duplication
0.084 1.00E-6 YJL092W SRS2 hom DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability
0.084 1.16E-6 YGL151W NUT1 hom Component of the RNA polymerase II mediator complex, which is required for transcriptional activation and also has a role in basal transcription