YDR081C / PDC2

Transcription factor for thiamine-regulated genes; required for expression of the two isoforms of pyruvate decarboxylase (PDC1 and PDC5) along with thiamine biosynthetic genes; binds a DNA sequence in the PDC5 promoter; mutant fails to grow on 2% glucose and thus is scored as inviable under standard conditions

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR081C / PDC2

Click on Significant Values for Screen Details ID:SGTC_122|Compound:0236-0016|FD-Score:4.29|P-value:5.31E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:4.53|P-value:2.70E-4 ID:SGTC_197|Compound:4544-0043|FD-Score:6.69|P-value:1.55E-7 ID:SGTC_490|Compound:phenamil|FD-Score:-4.10|P-value:7.89E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:8.79|P-value:8.69E-12 ID:SGTC_543|Compound:3844-1095|FD-Score:5.33|P-value:2.35E-5 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:-4.04|P-value:9.28E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:7.73|P-value:1.71E-9 ID:SGTC_666|Compound:0272-0154|FD-Score:4.21|P-value:6.58E-4 ID:SGTC_726|Compound:3448-4391|FD-Score:4.20|P-value:6.71E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:4.87|P-value:1.01E-4 ID:SGTC_734|Compound:2023-0417|FD-Score:5.89|P-value:3.37E-6 ID:SGTC_743|Compound:1275-0778|FD-Score:-5.46|P-value:1.30E-5 ID:SGTC_766|Compound:1391-0721|FD-Score:-4.01|P-value:9.82E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:4.08|P-value:9.25E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:6.00|P-value:2.28E-6 ID:SGTC_958|Compound:1222-0007|FD-Score:4.56|P-value:2.51E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:4.11|P-value:8.63E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:6.54|P-value:2.88E-7 ID:SGTC_1119|Compound:parthenolide|FD-Score:-5.28|P-value:2.40E-5 ID:SGTC_1124|Compound:fisetin|FD-Score:-7.14|P-value:1.98E-8 ID:SGTC_1230|Compound:0170-0365|FD-Score:7.07|P-value:3.23E-8 ID:SGTC_1235|Compound:benzylparaben|FD-Score:4.27|P-value:5.62E-4 ID:SGTC_1723|Compound:st034307|FD-Score:5.05|P-value:5.74E-5 ID:SGTC_1754|Compound:st042065|FD-Score:5.28|P-value:2.76E-5 ID:SGTC_1800|Compound:st050079|FD-Score:6.19|P-value:1.14E-6 ID:SGTC_1968|Compound:st069590|FD-Score:5.97|P-value:2.51E-6 ID:SGTC_2000|Compound:st075254|FD-Score:-4.49|P-value:2.72E-4 ID:SGTC_2006|Compound:4011487|FD-Score:4.75|P-value:1.44E-4 ID:SGTC_2021|Compound:4019055|FD-Score:5.50|P-value:1.31E-5 ID:SGTC_2036|Compound:5160441|FD-Score:7.08|P-value:3.14E-8 ID:SGTC_2139|Compound:5578692|FD-Score:-4.57|P-value:2.11E-4 ID:SGTC_2482|Compound:5981709|FD-Score:5.13|P-value:4.53E-5 ID:SGTC_2810|Compound:7959871|FD-Score:5.74|P-value:5.80E-6 ID:SGTC_2867|Compound:9039098|FD-Score:4.73|P-value:1.52E-4 ID:SGTC_2942|Compound:9052777|FD-Score:4.53|P-value:2.72E-4 ID:SGTC_3211|Compound:9128781|FD-Score:4.28|P-value:5.48E-4 ID:SGTC_3228|Compound:9131799|FD-Score:4.22|P-value:6.39E-4 ID:SGTC_3334|Compound:9142812|FD-Score:4.19|P-value:7.02E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:4.29|P-value:5.31E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:4.53|P-value:2.70E-4 ID:SGTC_197|Compound:4544-0043|FD-Score:6.69|P-value:1.55E-7 ID:SGTC_490|Compound:phenamil|FD-Score:-4.10|P-value:7.89E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:8.79|P-value:8.69E-12 ID:SGTC_543|Compound:3844-1095|FD-Score:5.33|P-value:2.35E-5 ID:SGTC_546|Compound:peri-Naphthalide|FD-Score:-4.04|P-value:9.28E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:7.73|P-value:1.71E-9 ID:SGTC_666|Compound:0272-0154|FD-Score:4.21|P-value:6.58E-4 ID:SGTC_726|Compound:3448-4391|FD-Score:4.20|P-value:6.71E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:4.87|P-value:1.01E-4 ID:SGTC_734|Compound:2023-0417|FD-Score:5.89|P-value:3.37E-6 ID:SGTC_743|Compound:1275-0778|FD-Score:-5.46|P-value:1.30E-5 ID:SGTC_766|Compound:1391-0721|FD-Score:-4.01|P-value:9.82E-4 ID:SGTC_916|Compound:4466-0038|FD-Score:4.08|P-value:9.25E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:6.00|P-value:2.28E-6 ID:SGTC_958|Compound:1222-0007|FD-Score:4.56|P-value:2.51E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:4.11|P-value:8.63E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:6.54|P-value:2.88E-7 ID:SGTC_1119|Compound:parthenolide|FD-Score:-5.28|P-value:2.40E-5 ID:SGTC_1124|Compound:fisetin|FD-Score:-7.14|P-value:1.98E-8 ID:SGTC_1230|Compound:0170-0365|FD-Score:7.07|P-value:3.23E-8 ID:SGTC_1235|Compound:benzylparaben|FD-Score:4.27|P-value:5.62E-4 ID:SGTC_1723|Compound:st034307|FD-Score:5.05|P-value:5.74E-5 ID:SGTC_1754|Compound:st042065|FD-Score:5.28|P-value:2.76E-5 ID:SGTC_1800|Compound:st050079|FD-Score:6.19|P-value:1.14E-6 ID:SGTC_1968|Compound:st069590|FD-Score:5.97|P-value:2.51E-6 ID:SGTC_2000|Compound:st075254|FD-Score:-4.49|P-value:2.72E-4 ID:SGTC_2006|Compound:4011487|FD-Score:4.75|P-value:1.44E-4 ID:SGTC_2021|Compound:4019055|FD-Score:5.50|P-value:1.31E-5 ID:SGTC_2036|Compound:5160441|FD-Score:7.08|P-value:3.14E-8 ID:SGTC_2139|Compound:5578692|FD-Score:-4.57|P-value:2.11E-4 ID:SGTC_2482|Compound:5981709|FD-Score:5.13|P-value:4.53E-5 ID:SGTC_2810|Compound:7959871|FD-Score:5.74|P-value:5.80E-6 ID:SGTC_2867|Compound:9039098|FD-Score:4.73|P-value:1.52E-4 ID:SGTC_2942|Compound:9052777|FD-Score:4.53|P-value:2.72E-4 ID:SGTC_3211|Compound:9128781|FD-Score:4.28|P-value:5.48E-4 ID:SGTC_3228|Compound:9131799|FD-Score:4.22|P-value:6.39E-4 ID:SGTC_3334|Compound:9142812|FD-Score:4.19|P-value:7.02E-4

Top fitness defect scores for YDR081C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_524 r073-0010 8.79 27.8 µM 8.69E-12 3 7.39E-19 1.83
2 SGTC_649 1123-0311 7.73 367.0 µM 1.71E-9 endomembrane recycling 4 5.53E-15 0.04
3 SGTC_2036 5160441 7.08 105.0 µM 3.14E-8 NEO1 9 7.44E-13 0.07
4 SGTC_1230 0170-0365 7.07 3.8 µM 3.23E-8 endomembrane recycling 9 7.77E-13 0.79
5 SGTC_197 4544-0043 6.69 41.6 µM 1.55E-7 endomembrane recycling 9 1.09E-11 0.83
6 SGTC_1026 k007-1105 6.54 30.6 µM 2.88E-7 ERAD & cell cycle 11 3.09E-11 0.05
7 SGTC_1800 st050079 6.19 29.1 µM 1.14E-6 NEO1-PIK1 7 3.10E-10 0.16
8 SGTC_951 1171-0579 6.00 82.7 µM 2.28E-6 cell wall 8 9.99E-10 0.39
9 SGTC_1968 st069590 5.97 43.6 µM 2.51E-6 8 1.17E-9 0.42
10 SGTC_734 2023-0417 5.89 80.0 µM 3.37E-6 ergosterol biosynthesis 2 1.92E-9 0.48
11 SGTC_2810 7959871 5.74 45.5 µM 5.80E-6 copper-dependent oxidative stress 8 4.77E-9 0.39
12 SGTC_2021 4019055 5.50 173.0 µM 1.31E-5 endomembrane recycling 22 1.87E-8 0.16
13 SGTC_543 3844-1095 5.33 191.0 µM 2.35E-5 ERG2 2 4.95E-8 0.90
14 SGTC_1754 st042065 5.28 16.1 µM 2.76E-5 NEO1-PIK1 16 6.49E-8 0.07
15 SGTC_2482 5981709 5.13 142.7 µM 4.53E-5 PDR1 7 1.49E-7 0.07
16 SGTC_1723 st034307 5.05 2.5 µM 5.74E-5 mitochondrial processes 9 2.21E-7 0.25
17 SGTC_733 4069-0007 4.87 56.1 µM 1.01E-4 ERAD & cell cycle 15 5.63E-7 0.29
18 SGTC_2006 4011487 4.75 200.0 µM 1.44E-4 endomembrane recycling 12 1.02E-6 0.24
19 SGTC_2867 9039098 4.73 71.4 µM 1.52E-4 iron homeostasis 7 1.12E-6 0.17
20 SGTC_958 1222-0007 4.56 33.0 µM 2.51E-4 1 2.58E-6 0.36

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.366 4.31E-107 YDR526C_d YDR526C_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.231 8.73E-42 YNL267W PIK1 het Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
0.203 1.54E-32 YDR084C TVP23 hom Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
0.203 2.50E-32 YCR093W CDC39 het Component of the CCR4-NOT complex, which has multiple roles in regulating mRNA levels including regulation of transcription and destabilizing mRNAs by deadenylation; basal transcription factor
0.190 1.25E-28 YGL199C_d YGL199C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YIP4/YGL198W
0.189 3.18E-28 YML037C_p YML037C_p hom Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
0.186 2.11E-27 YOR115C TRS33 hom One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
0.171 1.78E-23 YDR372C VPS74 hom Golgi phosphatidylinositol-4-kinase effector and PtdIns4P sensor; interacts with the cytosolic domains of cis and medial glycosyltransferases, and in the PtdIns4P-bound state mediates the targeting of these enzymes to the Golgi; interacts with the catalytic domain of Sac1p, the major cellular PtdIns4P phosphatase, to direct dephosphosphorylation of the Golgi pool of PtdIns4P; tetramerization required for function; ortholog of human GOLPH3/GPP34/GMx33
0.170 5.85E-23 YDL090C RAM1 hom Beta subunit of the CAAX farnesyltransferase (FTase) that prenylates the a-factor mating pheromone and Ras proteins; required for the membrane localization of Ras proteins and a-factor; homolog of the mammalian FTase beta subunit
0.169 7.77E-23 YPR091C NVJ2 hom Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments
0.163 1.95E-21 YHR007C ERG11 het Lanosterol 14-alpha-demethylase; catalyzes the C-14 demethylation of lanosterol to form 4,4''-dimethyl cholesta-8,14,24-triene-3-beta-ol in the ergosterol biosynthesis pathway; member of the cytochrome P450 family; associated and coordinately regulated with the P450 reductase Ncp1p
0.163 2.30E-21 YMR134W ERG29 het Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes
0.151 1.08E-18 YGL161C YIP5 hom Protein that interacts with Rab GTPases, localized to late Golgi vesicles; computational analysis of large-scale protein-protein interaction data suggests a possible role in vesicle-mediated transport
0.149 3.25E-18 YOR357C SNX3 hom Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p
0.145 3.68E-17 YGR166W TRS65 hom Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response