YDR083W / RRP8

Nucleolar S-adenosylmethionine-dependent rRNA methyltransferase; methylates adenine (m1A) of the large subunit (LSU) rRNA at position 645; involved in pre-rRNA cleavage at site A2; mutation is synthetically lethal with a gar1 mutation; deletion disrupts telomere maintenance by influencing the expression of neighboring gene STN1

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR083W / RRP8

Click on Significant Values for Screen Details ID:SGTC_37|Compound:0566-0070|FD-Score:3.83|P-value:6.48E-5 ID:SGTC_41|Compound:0478-0632|FD-Score:3.15|P-value:7.75E-4 ID:SGTC_62|Compound:0180-0423|FD-Score:4.35|P-value:7.38E-6 ID:SGTC_97|Compound:0535-0701|FD-Score:3.46|P-value:2.66E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:4.96|P-value:4.09E-7 ID:SGTC_135|Compound:0933-0004|FD-Score:3.93|P-value:4.28E-5 ID:SGTC_159|Compound:3970-0959|FD-Score:4.09|P-value:2.27E-5 ID:SGTC_160|Compound:3453-0671|FD-Score:3.13|P-value:8.26E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:3.67|P-value:1.24E-4 ID:SGTC_256|Compound:clotrimazole|FD-Score:-4.08|P-value:4.63E-5 ID:SGTC_262|Compound:fenpropimorph|FD-Score:-3.34|P-value:7.32E-4 ID:SGTC_297|Compound:0986-0032|FD-Score:3.97|P-value:3.64E-5 ID:SGTC_344|Compound:k064-0035|FD-Score:5.14|P-value:1.67E-7 ID:SGTC_490|Compound:phenamil|FD-Score:-3.28|P-value:8.93E-4 ID:SGTC_521|Compound:r062-0005|FD-Score:5.13|P-value:1.72E-7 ID:SGTC_582|Compound:0987-0078|FD-Score:3.37|P-value:3.64E-4 ID:SGTC_585|Compound:0kpi-0023|FD-Score:-3.80|P-value:1.42E-4 ID:SGTC_618|Compound:k072-0232|FD-Score:4.67|P-value:1.69E-6 ID:SGTC_625|Compound:k213-0058|FD-Score:3.23|P-value:6.00E-4 ID:SGTC_788|Compound:1182-0090|FD-Score:3.53|P-value:2.07E-4 ID:SGTC_832|Compound:4083-0019|FD-Score:4.42|P-value:5.35E-6 ID:SGTC_972|Compound:1319-0219|FD-Score:4.05|P-value:2.69E-5 ID:SGTC_1016|Compound:4226-1401|FD-Score:3.43|P-value:2.92E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:3.28|P-value:4.99E-4 ID:SGTC_1070|Compound:indatraline|FD-Score:3.95|P-value:4.03E-5 ID:SGTC_1081|Compound:sertraline|FD-Score:3.86|P-value:5.76E-5 ID:SGTC_1130|Compound:3454-2858|FD-Score:4.25|P-value:1.11E-5 ID:SGTC_1188|Compound:1403-0014|FD-Score:-3.90|P-value:9.47E-5 ID:SGTC_1224|Compound:0823-0416|FD-Score:3.11|P-value:9.04E-4 ID:SGTC_1269|Compound:0831-0796|FD-Score:4.65|P-value:1.84E-6 ID:SGTC_1344|Compound:1488-0097|FD-Score:-3.28|P-value:9.05E-4 ID:SGTC_1406|Compound:3909-7906|FD-Score:3.38|P-value:3.58E-4 ID:SGTC_1407|Compound:3909-7909|FD-Score:5.07|P-value:2.39E-7 ID:SGTC_1459|Compound:k035-0031|FD-Score:-4.27|P-value:2.10E-5 ID:SGTC_1492|Compound:4158-0558|FD-Score:3.08|P-value:9.82E-4 ID:SGTC_1535|Compound:tetrahydropapaveroline hydrobromide|FD-Score:3.17|P-value:7.23E-4 ID:SGTC_1664|Compound:st014217|FD-Score:6.15|P-value:5.54E-10 ID:SGTC_1761|Compound:st044296|FD-Score:4.12|P-value:2.00E-5 ID:SGTC_1765|Compound:st044815|FD-Score:4.33|P-value:7.94E-6 ID:SGTC_1899|Compound:st063254|FD-Score:4.72|P-value:1.31E-6 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.69|P-value:2.15E-4 ID:SGTC_1958|Compound:st076513|FD-Score:6.67|P-value:1.98E-11 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:-4.77|P-value:2.29E-6 ID:SGTC_2300|Compound:7976697|FD-Score:6.72|P-value:1.48E-11 ID:SGTC_2404|Compound:5161160|FD-Score:3.23|P-value:5.92E-4 ID:SGTC_2415|Compound:st077333|FD-Score:-3.55|P-value:3.55E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.87|P-value:5.52E-5 ID:SGTC_2619|Compound:obliquin|FD-Score:-4.16|P-value:3.40E-5 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:-4.28|P-value:2.04E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.34|P-value:5.83E-8 ID:SGTC_2729|Compound:fluphenazine|FD-Score:3.11|P-value:8.96E-4 ID:SGTC_2846|Compound:9008642|FD-Score:4.00|P-value:3.26E-5 ID:SGTC_2870|Compound:9038528|FD-Score:3.87|P-value:5.45E-5 ID:SGTC_2975|Compound:9084847|FD-Score:3.89|P-value:5.20E-5 ID:SGTC_3032|Compound:9089427|FD-Score:5.03|P-value:2.87E-7 ID:SGTC_3059|Compound:1327-0034|FD-Score:3.15|P-value:7.79E-4 ID:SGTC_3077|Compound:9117319|FD-Score:4.05|P-value:2.69E-5 ID:SGTC_3149|Compound:9097855|FD-Score:-4.50|P-value:7.96E-6 ID:SGTC_3150|Compound:9097574|FD-Score:-4.09|P-value:4.49E-5 ID:SGTC_3152|Compound:9099253|FD-Score:-3.79|P-value:1.48E-4 ID:SGTC_3280|Compound:9140961|FD-Score:3.76|P-value:8.46E-5 ID:SGTC_3282|Compound:9108498|FD-Score:-3.29|P-value:8.56E-4 ID:SGTC_3283|Compound:9109332|FD-Score:-4.41|P-value:1.19E-5 ID:SGTC_37|Compound:0566-0070|FD-Score:3.83|P-value:6.48E-5 ID:SGTC_41|Compound:0478-0632|FD-Score:3.15|P-value:7.75E-4 ID:SGTC_62|Compound:0180-0423|FD-Score:4.35|P-value:7.38E-6 ID:SGTC_97|Compound:0535-0701|FD-Score:3.46|P-value:2.66E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:4.96|P-value:4.09E-7 ID:SGTC_135|Compound:0933-0004|FD-Score:3.93|P-value:4.28E-5 ID:SGTC_159|Compound:3970-0959|FD-Score:4.09|P-value:2.27E-5 ID:SGTC_160|Compound:3453-0671|FD-Score:3.13|P-value:8.26E-4 ID:SGTC_163|Compound:1327-0036|FD-Score:3.67|P-value:1.24E-4 ID:SGTC_256|Compound:clotrimazole|FD-Score:-4.08|P-value:4.63E-5 ID:SGTC_262|Compound:fenpropimorph|FD-Score:-3.34|P-value:7.32E-4 ID:SGTC_297|Compound:0986-0032|FD-Score:3.97|P-value:3.64E-5 ID:SGTC_344|Compound:k064-0035|FD-Score:5.14|P-value:1.67E-7 ID:SGTC_490|Compound:phenamil|FD-Score:-3.28|P-value:8.93E-4 ID:SGTC_521|Compound:r062-0005|FD-Score:5.13|P-value:1.72E-7 ID:SGTC_582|Compound:0987-0078|FD-Score:3.37|P-value:3.64E-4 ID:SGTC_585|Compound:0kpi-0023|FD-Score:-3.80|P-value:1.42E-4 ID:SGTC_618|Compound:k072-0232|FD-Score:4.67|P-value:1.69E-6 ID:SGTC_625|Compound:k213-0058|FD-Score:3.23|P-value:6.00E-4 ID:SGTC_788|Compound:1182-0090|FD-Score:3.53|P-value:2.07E-4 ID:SGTC_832|Compound:4083-0019|FD-Score:4.42|P-value:5.35E-6 ID:SGTC_972|Compound:1319-0219|FD-Score:4.05|P-value:2.69E-5 ID:SGTC_1016|Compound:4226-1401|FD-Score:3.43|P-value:2.92E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:3.28|P-value:4.99E-4 ID:SGTC_1070|Compound:indatraline|FD-Score:3.95|P-value:4.03E-5 ID:SGTC_1081|Compound:sertraline|FD-Score:3.86|P-value:5.76E-5 ID:SGTC_1130|Compound:3454-2858|FD-Score:4.25|P-value:1.11E-5 ID:SGTC_1188|Compound:1403-0014|FD-Score:-3.90|P-value:9.47E-5 ID:SGTC_1224|Compound:0823-0416|FD-Score:3.11|P-value:9.04E-4 ID:SGTC_1269|Compound:0831-0796|FD-Score:4.65|P-value:1.84E-6 ID:SGTC_1344|Compound:1488-0097|FD-Score:-3.28|P-value:9.05E-4 ID:SGTC_1406|Compound:3909-7906|FD-Score:3.38|P-value:3.58E-4 ID:SGTC_1407|Compound:3909-7909|FD-Score:5.07|P-value:2.39E-7 ID:SGTC_1459|Compound:k035-0031|FD-Score:-4.27|P-value:2.10E-5 ID:SGTC_1492|Compound:4158-0558|FD-Score:3.08|P-value:9.82E-4 ID:SGTC_1535|Compound:tetrahydropapaveroline hydrobromide|FD-Score:3.17|P-value:7.23E-4 ID:SGTC_1664|Compound:st014217|FD-Score:6.15|P-value:5.54E-10 ID:SGTC_1761|Compound:st044296|FD-Score:4.12|P-value:2.00E-5 ID:SGTC_1765|Compound:st044815|FD-Score:4.33|P-value:7.94E-6 ID:SGTC_1899|Compound:st063254|FD-Score:4.72|P-value:1.31E-6 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.69|P-value:2.15E-4 ID:SGTC_1958|Compound:st076513|FD-Score:6.67|P-value:1.98E-11 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:-4.77|P-value:2.29E-6 ID:SGTC_2300|Compound:7976697|FD-Score:6.72|P-value:1.48E-11 ID:SGTC_2404|Compound:5161160|FD-Score:3.23|P-value:5.92E-4 ID:SGTC_2415|Compound:st077333|FD-Score:-3.55|P-value:3.55E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.87|P-value:5.52E-5 ID:SGTC_2619|Compound:obliquin|FD-Score:-4.16|P-value:3.40E-5 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:-4.28|P-value:2.04E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.34|P-value:5.83E-8 ID:SGTC_2729|Compound:fluphenazine|FD-Score:3.11|P-value:8.96E-4 ID:SGTC_2846|Compound:9008642|FD-Score:4.00|P-value:3.26E-5 ID:SGTC_2870|Compound:9038528|FD-Score:3.87|P-value:5.45E-5 ID:SGTC_2975|Compound:9084847|FD-Score:3.89|P-value:5.20E-5 ID:SGTC_3032|Compound:9089427|FD-Score:5.03|P-value:2.87E-7 ID:SGTC_3059|Compound:1327-0034|FD-Score:3.15|P-value:7.79E-4 ID:SGTC_3077|Compound:9117319|FD-Score:4.05|P-value:2.69E-5 ID:SGTC_3149|Compound:9097855|FD-Score:-4.50|P-value:7.96E-6 ID:SGTC_3150|Compound:9097574|FD-Score:-4.09|P-value:4.49E-5 ID:SGTC_3152|Compound:9099253|FD-Score:-3.79|P-value:1.48E-4 ID:SGTC_3280|Compound:9140961|FD-Score:3.76|P-value:8.46E-5 ID:SGTC_3282|Compound:9108498|FD-Score:-3.29|P-value:8.56E-4 ID:SGTC_3283|Compound:9109332|FD-Score:-4.41|P-value:1.19E-5

Top fitness defect scores for YDR083W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2300 7976697 6.72 71.6 µM 1.48E-11 ERG2 12 9.24E-12
2 SGTC_1958 st076513 6.67 41.9 µM 1.98E-11 60S ribosome export 7 1.25E-11
3 SGTC_1664 st014217 6.15 9.0 µM 5.54E-10 60S ribosome export 2 3.90E-10
4 SGTC_2688 kanamycin b 5.34 96.7 µM 5.83E-8 calcium & mitochondrial duress 216 4.74E-8
5 SGTC_344 k064-0035 5.14 6.4 µM 1.67E-7 60S ribosome export 9 1.40E-7
6 SGTC_521 r062-0005 5.13 13.8 µM 1.72E-7 5 1.44E-7
7 SGTC_1407 3909-7909 5.07 12.3 µM 2.39E-7 60S ribosome export 4 2.03E-7
8 SGTC_3032 9089427 5.03 49.5 µM 2.87E-7 60S ribosome export 5 2.44E-7
9 SGTC_118 0083-0097 4.96 135.3 µM 4.09E-7 translation 60 3.52E-7
10 SGTC_1899 st063254 4.72 59.6 µM 1.31E-6 4 1.17E-6
11 SGTC_618 k072-0232 4.67 33.2 µM 1.69E-6 60S ribosome export 1 1.51E-6
12 SGTC_1269 0831-0796 4.65 3.0 µM 1.84E-6 60S ribosome export 5 1.65E-6
13 SGTC_832 4083-0019 4.42 164.0 µM 5.35E-6 translation 65 4.94E-6
14 SGTC_62 0180-0423 4.35 3.2 µM 7.38E-6 60S ribosome export 11 6.88E-6
15 SGTC_1765 st044815 4.33 90.4 µM 7.94E-6 16 7.42E-6
16 SGTC_1130 3454-2858 4.25 15.9 µM 1.11E-5 NEO1 71 1.05E-5
17 SGTC_1761 st044296 4.12 37.0 µM 2.00E-5 11 1.91E-5
18 SGTC_159 3970-0959 4.09 50.0 µM 2.27E-5 70 2.18E-5
19 SGTC_972 1319-0219 4.05 195.0 µM 2.69E-5 25 2.59E-5
20 SGTC_3077 9117319 4.05 49.5 µM 2.69E-5 18 2.59E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.234 6.51E-43 YGL099W LSG1 het Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
0.193 1.91E-29 YBR267W REI1 hom Cytoplasmic pre-60S factor; required for the correct recycling of shuttling factors Alb1, Arx1 and Tif6 at the end of the ribosomal large subunit biogenesis; involved in bud growth in the mitotic signaling network
0.154 2.99E-19 YHR170W NMD3 het Protein involved in nuclear export of the large ribosomal subunit; acts as a Crm1p-dependent adapter protein for export of nascent ribosomal subunits through the nuclear pore complex
0.152 7.64E-19 YNL227C JJJ1 hom Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis
0.138 1.00E-15 YLR061W RPL22A hom Ribosomal 60S subunit protein L22A; required for the oxidative stress response in yeast; homologous to mammalian ribosomal protein L22, no bacterial homolog; RPL22A has a paralog, RPL22B, that arose from the whole genome duplication
0.133 9.01E-15 YDR140W MTQ2 hom S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC
0.127 1.73E-13 YDL082W RPL13A hom Ribosomal 60S subunit protein L13A; not essential for viability; homologous to mammalian ribosomal protein L13, no bacterial homolog; RPL13A has a paralog, RPL13B, that arose from the whole genome duplication
0.124 5.54E-13 YEL054C RPL12A hom Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication
0.123 8.20E-13 YLR062C_d BUD28_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene RPL22A; diploid mutant displays a weak budding pattern phenotype in a systematic assay
0.120 3.21E-12 YJL188C_d BUD19_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay
0.119 4.58E-12 YOR309C_d YOR309C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58
0.117 8.77E-12 YGR214W RPS0A hom Ribosomal 40S subunit protein S0A; required for maturation of 18S rRNA along with Rps0Bp; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2; RPS0A has a paralog, RPS0B, that arose from the whole genome duplication;
0.112 8.98E-11 YBR191W RPL21A hom Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication
0.109 2.28E-10 YER060W-A FCY22 hom Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function
0.103 1.93E-9 YGR162W TIF4631 hom Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication