YDR091C / RLI1

Essential iron-sulfur protein required for ribosome biogenesis and translation initiation and termination; facilitates binding of a multifactor complex (MFC) of initiation factors to the small ribosomal subunit; predicted ABC family ATPase; forms a complex with Lto1p and Yae1p that may be involved in protection of the ribosome from damage due to reactive oxygen species

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR091C / RLI1

Click on Significant Values for Screen Details ID:SGTC_273|Compound:hydroxyurea|FD-Score:4.59|P-value:3.89E-5 ID:SGTC_301|Compound:4119-0100|FD-Score:3.73|P-value:6.71E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:-3.96|P-value:3.23E-4 ID:SGTC_373|Compound:0844-0104|FD-Score:-3.82|P-value:4.86E-4 ID:SGTC_582|Compound:0987-0078|FD-Score:-3.59|P-value:9.75E-4 ID:SGTC_586|Compound:0957-0265|FD-Score:6.20|P-value:4.75E-8 ID:SGTC_669|Compound:0108-0021|FD-Score:-4.80|P-value:1.73E-5 ID:SGTC_852|Compound:0418-0143|FD-Score:-3.81|P-value:5.07E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:4.88|P-value:1.32E-5 ID:SGTC_947|Compound:3447-0010|FD-Score:3.73|P-value:6.59E-4 ID:SGTC_1136|Compound:pergolide|FD-Score:3.88|P-value:4.28E-4 ID:SGTC_1674|Compound:st016616|FD-Score:4.10|P-value:2.14E-4 ID:SGTC_1676|Compound:st018489|FD-Score:3.64|P-value:8.65E-4 ID:SGTC_1766|Compound:st045414|FD-Score:4.36|P-value:8.84E-5 ID:SGTC_1930|Compound:st074634|FD-Score:-3.71|P-value:6.82E-4 ID:SGTC_1959|Compound:st077091|FD-Score:4.33|P-value:9.71E-5 ID:SGTC_1999|Compound:st074831|FD-Score:3.76|P-value:6.08E-4 ID:SGTC_2158|Compound:5657123|FD-Score:-4.59|P-value:3.83E-5 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:4.22|P-value:1.44E-4 ID:SGTC_2296|Compound:7971656|FD-Score:-3.76|P-value:5.91E-4 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:3.73|P-value:6.65E-4 ID:SGTC_2601|Compound:securinine|FD-Score:4.45|P-value:6.38E-5 ID:SGTC_2619|Compound:obliquin|FD-Score:3.63|P-value:9.04E-4 ID:SGTC_2624|Compound:nerol|FD-Score:-3.66|P-value:7.98E-4 ID:SGTC_2784|Compound:7567673|FD-Score:3.74|P-value:6.46E-4 ID:SGTC_2796|Compound:7949634|FD-Score:-4.22|P-value:1.38E-4 ID:SGTC_2797|Compound:7859098|FD-Score:6.41|P-value:1.69E-8 ID:SGTC_2867|Compound:9039098|FD-Score:4.43|P-value:6.98E-5 ID:SGTC_2969|Compound:9091408|FD-Score:3.93|P-value:3.57E-4 ID:SGTC_3025|Compound:9087895|FD-Score:-4.52|P-value:4.85E-5 ID:SGTC_3136|Compound:9095672|FD-Score:-3.84|P-value:4.59E-4 ID:SGTC_3144|Compound:9087057|FD-Score:-5.19|P-value:3.80E-6 ID:SGTC_3266|Compound:9136860|FD-Score:-4.18|P-value:1.56E-4 ID:SGTC_273|Compound:hydroxyurea|FD-Score:4.59|P-value:3.89E-5 ID:SGTC_301|Compound:4119-0100|FD-Score:3.73|P-value:6.71E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:-3.96|P-value:3.23E-4 ID:SGTC_373|Compound:0844-0104|FD-Score:-3.82|P-value:4.86E-4 ID:SGTC_582|Compound:0987-0078|FD-Score:-3.59|P-value:9.75E-4 ID:SGTC_586|Compound:0957-0265|FD-Score:6.20|P-value:4.75E-8 ID:SGTC_669|Compound:0108-0021|FD-Score:-4.80|P-value:1.73E-5 ID:SGTC_852|Compound:0418-0143|FD-Score:-3.81|P-value:5.07E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:4.88|P-value:1.32E-5 ID:SGTC_947|Compound:3447-0010|FD-Score:3.73|P-value:6.59E-4 ID:SGTC_1136|Compound:pergolide|FD-Score:3.88|P-value:4.28E-4 ID:SGTC_1674|Compound:st016616|FD-Score:4.10|P-value:2.14E-4 ID:SGTC_1676|Compound:st018489|FD-Score:3.64|P-value:8.65E-4 ID:SGTC_1766|Compound:st045414|FD-Score:4.36|P-value:8.84E-5 ID:SGTC_1930|Compound:st074634|FD-Score:-3.71|P-value:6.82E-4 ID:SGTC_1959|Compound:st077091|FD-Score:4.33|P-value:9.71E-5 ID:SGTC_1999|Compound:st074831|FD-Score:3.76|P-value:6.08E-4 ID:SGTC_2158|Compound:5657123|FD-Score:-4.59|P-value:3.83E-5 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:4.22|P-value:1.44E-4 ID:SGTC_2296|Compound:7971656|FD-Score:-3.76|P-value:5.91E-4 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:3.73|P-value:6.65E-4 ID:SGTC_2601|Compound:securinine|FD-Score:4.45|P-value:6.38E-5 ID:SGTC_2619|Compound:obliquin|FD-Score:3.63|P-value:9.04E-4 ID:SGTC_2624|Compound:nerol|FD-Score:-3.66|P-value:7.98E-4 ID:SGTC_2784|Compound:7567673|FD-Score:3.74|P-value:6.46E-4 ID:SGTC_2796|Compound:7949634|FD-Score:-4.22|P-value:1.38E-4 ID:SGTC_2797|Compound:7859098|FD-Score:6.41|P-value:1.69E-8 ID:SGTC_2867|Compound:9039098|FD-Score:4.43|P-value:6.98E-5 ID:SGTC_2969|Compound:9091408|FD-Score:3.93|P-value:3.57E-4 ID:SGTC_3025|Compound:9087895|FD-Score:-4.52|P-value:4.85E-5 ID:SGTC_3136|Compound:9095672|FD-Score:-3.84|P-value:4.59E-4 ID:SGTC_3144|Compound:9087057|FD-Score:-5.19|P-value:3.80E-6 ID:SGTC_3266|Compound:9136860|FD-Score:-4.18|P-value:1.56E-4

Top fitness defect scores for YDR091C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2797 7859098 6.41 81.8 µM 1.69E-8 1 7.05E-11 0.81
2 SGTC_586 0957-0265 6.20 8.2 µM 4.75E-8 cell wall 20 2.81E-10 0.16
3 SGTC_925 2064-1272 4.88 59.6 µM 1.32E-5 3 5.19E-7 0.29
4 SGTC_273 hydroxyurea 4.59 18.1 mM 3.89E-5 heme biosynthesis & mitochondrial translocase 18 2.19E-6 0.17
5 SGTC_2601 securinine 4.45 100.0 µM 6.38E-5 13 4.22E-6 0.05
6 SGTC_2867 9039098 4.43 71.4 µM 6.98E-5 iron homeostasis 10 4.76E-6 0.21
7 SGTC_1766 st045414 4.36 44.8 µM 8.84E-5 RPP1 & pyrimidine depletion 9 6.52E-6 0.05
8 SGTC_1959 st077091 4.33 55.8 µM 9.71E-5 RPP1 & pyrimidine depletion 21 7.39E-6 0.18
9 SGTC_2169 5-(ethoxymethyl)quinolin-8-ol 4.22 49.5 µM 1.44E-4 19 1.25E-5 0.07
10 SGTC_1674 st016616 4.10 23.6 µM 2.14E-4 7 2.11E-5 0.39
11 SGTC_2969 9091408 3.93 28.7 µM 3.57E-4 6 4.18E-5 0.11
12 SGTC_1136 pergolide 3.88 1.1 µM 4.28E-4 20 5.30E-5 0.02
13 SGTC_1999 st074831 3.76 12.5 µM 6.08E-4 plasma membrane duress 14 8.44E-5 0.01
14 SGTC_2784 7567673 3.74 72.7 µM 6.46E-4 iron homeostasis 16 9.16E-5 0.09
15 SGTC_947 3447-0010 3.73 211.0 µM 6.59E-4 5 9.40E-5 0.19
16 SGTC_2520 obtusaquinone 3.73 4.7 µM 6.65E-4 7 9.51E-5 0.10
17 SGTC_301 4119-0100 3.73 24.5 µM 6.71E-4 NEO1 8 9.62E-5 0.05
18 SGTC_1676 st018489 3.64 23.4 µM 8.65E-4 60S ribosome export 6 1.35E-4 0.09
19 SGTC_2619 obliquin 3.63 87.9 µM 9.04E-4 cell wall 34 1.43E-4 0.00
20 SGTC_2472 5377652 3.56 49.4 µM 0.00111 TRP & mitochondrial translation 16 1.87E-4 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.121 1.97E-12 YJL159W HSP150 hom O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
0.114 3.55E-11 YJL027C_p YJL027C_p hom Putative protein of unknown function
0.108 3.33E-10 YPL190C NAB3 het RNA-binding protein, subunit of Nrd1 complex (Nrd1p-Nab3p-Sen1p); complex interacts with the exosome to mediate 3′ end formation of some mRNAs, snRNAs, snoRNAs, and CUTs; required for termination of non-poly(A) transcripts and efficient splicing
0.088 3.11E-7 YPL002C SNF8 hom Component of the ESCRT-II complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; appears to be functionally related to SNF7; involved in glucose derepression
0.088 3.60E-7 YKL052C ASK1 het Essential subunit of the Dam1 complex (aka DASH complex); couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; phosphorylated during the cell cycle by cyclin-dependent kinases; protein abundance increases in response to DNA replication stress
0.086 6.82E-7 YJR046W TAH11 het DNA replication licensing factor, required for pre-replication complex assembly
0.084 9.84E-7 YBR143C SUP45 het Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p
0.084 1.13E-6 YIL083C CAB2 het Probable phosphopantothenoylcysteine synthetase (PPCS), which catalyzes the second step of coenzyme A biosynthesis from pantothenate; null mutant lethality is complemented by E. coli coaBC (encoding a bifunctional enzyme with PPCS activity)
0.083 1.54E-6 YKL145W RPT1 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for optimal CDC20 transcription; interacts with Rpn12p and Ubr1p; mutant has aneuploidy tolerance
0.082 1.80E-6 YDR207C UME6 hom Key transcriptional regulator of early meiotic genes, binds URS1 upstream regulatory sequence, couples metabolic responses to nutritional cues with initiation and progression of meiosis, forms complex with Ime1p, and also with Sin3p-Rpd3p
0.081 2.50E-6 YNL118C DCP2 het Catalytic subunit of the Dcp1p-Dcp2p decapping enzyme complex; removes the 5' cap structure from mRNAs prior to their degradation; nudix hydrolase family member; forms cytoplasmic foci upon DNA replication stress
0.081 2.69E-6 YKL016C ATP7 hom Subunit d of the stator stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis
0.079 4.45E-6 YNL085W MKT1 hom Protein that forms a complex with Pbp1p; complex may mediate posttranscriptional regulation of HO; involved in propagation of M2 dsRNA satellite of L-A virus; allelic variation affects mitochondrial genome stability, drug resistance, and more; forms cytoplasmic foci upon DNA replication stress
0.078 6.03E-6 YNL236W SIN4 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; contributes to both postive and negative transcriptional regulation; dispensible for basal transcription
0.078 6.59E-6 YDR327W_d YDR327W_d het Dubious open reading frame unlikely to encode a protein, based on experimental and comparative sequence data; partially overlaps the verified gene SKP1