YDR092W / UBC13

Ubiquitin-conjugating enzyme involved in the error-free DNA postreplication repair pathway; interacts with Mms2p to assemble ubiquitin chains at the Ub Lys-63 residue; DNA damage triggers redistribution from the cytoplasm to the nucleus

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR092W / UBC13

Click on Significant Values for Screen Details ID:SGTC_149|Compound:3970-0822|FD-Score:3.15|P-value:2.80E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:3.44|P-value:8.14E-5 ID:SGTC_168|Compound:3970-0862|FD-Score:4.38|P-value:7.70E-7 ID:SGTC_187|Compound:1488-2104|FD-Score:-2.91|P-value:5.39E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:7.25|P-value:6.54E-16 ID:SGTC_214|Compound:4373-3496|FD-Score:5.05|P-value:1.38E-8 ID:SGTC_248|Compound:streptozotocin|FD-Score:8.07|P-value:2.91E-19 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:16.70|P-value:9.72E-77 ID:SGTC_275|Compound:trichlorophene|FD-Score:3.07|P-value:3.92E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:5.15|P-value:7.62E-9 ID:SGTC_375|Compound:3970-0961|FD-Score:-5.11|P-value:6.11E-9 ID:SGTC_387|Compound:norethindrone acetate|FD-Score:4.06|P-value:4.10E-6 ID:SGTC_391|Compound:gliotoxin|FD-Score:3.88|P-value:1.02E-5 ID:SGTC_429|Compound:1112-0049|FD-Score:4.19|P-value:2.16E-6 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.93|P-value:1.93E-8 ID:SGTC_464|Compound:bw-b 70c|FD-Score:-3.55|P-value:3.47E-5 ID:SGTC_465|Compound:cgp-37157|FD-Score:-2.91|P-value:5.46E-4 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:3.44|P-value:8.22E-5 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:-3.67|P-value:2.01E-5 ID:SGTC_490|Compound:phenamil|FD-Score:3.53|P-value:5.35E-5 ID:SGTC_539|Compound:4859-0462|FD-Score:3.05|P-value:4.21E-4 ID:SGTC_550|Compound:4123-0385|FD-Score:5.10|P-value:9.96E-9 ID:SGTC_560|Compound:2332-1943|FD-Score:2.95|P-value:6.26E-4 ID:SGTC_641|Compound:4092-0595|FD-Score:-3.29|P-value:1.16E-4 ID:SGTC_702|Compound:1488-0190|FD-Score:3.83|P-value:1.30E-5 ID:SGTC_782|Compound:0083-0095|FD-Score:-2.87|P-value:6.34E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:10.00|P-value:9.04E-29 ID:SGTC_978|Compound:1416-0512|FD-Score:2.86|P-value:8.72E-4 ID:SGTC_1117|Compound:retinoic acid|FD-Score:3.12|P-value:3.22E-4 ID:SGTC_1186|Compound:1319-0216|FD-Score:-3.38|P-value:7.63E-5 ID:SGTC_1252|Compound:0493-0875|FD-Score:-3.05|P-value:3.20E-4 ID:SGTC_1266|Compound:0828-0227|FD-Score:3.10|P-value:3.40E-4 ID:SGTC_1425|Compound:4048-4433|FD-Score:7.28|P-value:5.06E-16 ID:SGTC_1455|Compound:4262-0120|FD-Score:3.17|P-value:2.58E-4 ID:SGTC_1590|Compound:st077108|FD-Score:3.10|P-value:3.49E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.72|P-value:2.30E-5 ID:SGTC_1889|Compound:st059080|FD-Score:4.81|P-value:6.06E-8 ID:SGTC_1897|Compound:st060201|FD-Score:5.23|P-value:4.52E-9 ID:SGTC_1900|Compound:st060200|FD-Score:3.39|P-value:1.03E-4 ID:SGTC_1936|Compound:st074407|FD-Score:5.91|P-value:3.99E-11 ID:SGTC_1942|Compound:st076330|FD-Score:2.88|P-value:8.15E-4 ID:SGTC_1953|Compound:st076800|FD-Score:-3.42|P-value:6.53E-5 ID:SGTC_2025|Compound:5147754|FD-Score:-2.76|P-value:9.56E-4 ID:SGTC_2027|Compound:5150920|FD-Score:-3.03|P-value:3.46E-4 ID:SGTC_2115|Compound:tiliquinol|FD-Score:-3.85|P-value:8.21E-6 ID:SGTC_2259|Compound:7964009|FD-Score:2.91|P-value:7.40E-4 ID:SGTC_2283|Compound:7944793|FD-Score:-2.98|P-value:4.19E-4 ID:SGTC_2296|Compound:7971656|FD-Score:-3.40|P-value:7.01E-5 ID:SGTC_2299|Compound:7976368|FD-Score:3.62|P-value:3.60E-5 ID:SGTC_2301|Compound:7769476|FD-Score:4.65|P-value:1.64E-7 ID:SGTC_2318|Compound:6029776|FD-Score:-2.93|P-value:5.17E-4 ID:SGTC_2351|Compound:9026858|FD-Score:-2.87|P-value:6.39E-4 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:2.83|P-value:9.93E-4 ID:SGTC_2535|Compound:4',4'-dimethoxydalbergione|FD-Score:5.71|P-value:1.66E-10 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:3.02|P-value:4.73E-4 ID:SGTC_2610|Compound:ononetin|FD-Score:-3.56|P-value:3.33E-5 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:3.08|P-value:3.83E-4 ID:SGTC_2677|Compound:mitomycin C|FD-Score:4.74|P-value:9.35E-8 ID:SGTC_2692|Compound:st077426|FD-Score:2.83|P-value:9.79E-4 ID:SGTC_2858|Compound:9025781|FD-Score:-2.90|P-value:5.69E-4 ID:SGTC_3233|Compound:9132340|FD-Score:2.95|P-value:6.23E-4 ID:SGTC_3254|Compound:9136651|FD-Score:2.85|P-value:9.13E-4 ID:SGTC_3328|Compound:9140624|FD-Score:4.04|P-value:4.67E-6 ID:SGTC_149|Compound:3970-0822|FD-Score:3.15|P-value:2.80E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:3.44|P-value:8.14E-5 ID:SGTC_168|Compound:3970-0862|FD-Score:4.38|P-value:7.70E-7 ID:SGTC_187|Compound:1488-2104|FD-Score:-2.91|P-value:5.39E-4 ID:SGTC_212|Compound:3807-4682|FD-Score:7.25|P-value:6.54E-16 ID:SGTC_214|Compound:4373-3496|FD-Score:5.05|P-value:1.38E-8 ID:SGTC_248|Compound:streptozotocin|FD-Score:8.07|P-value:2.91E-19 ID:SGTC_267|Compound:4-Nitroquinoline-1-oxide  (4-NQO)|FD-Score:16.70|P-value:9.72E-77 ID:SGTC_275|Compound:trichlorophene|FD-Score:3.07|P-value:3.92E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:5.15|P-value:7.62E-9 ID:SGTC_375|Compound:3970-0961|FD-Score:-5.11|P-value:6.11E-9 ID:SGTC_387|Compound:norethindrone acetate|FD-Score:4.06|P-value:4.10E-6 ID:SGTC_391|Compound:gliotoxin|FD-Score:3.88|P-value:1.02E-5 ID:SGTC_429|Compound:1112-0049|FD-Score:4.19|P-value:2.16E-6 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.93|P-value:1.93E-8 ID:SGTC_464|Compound:bw-b 70c|FD-Score:-3.55|P-value:3.47E-5 ID:SGTC_465|Compound:cgp-37157|FD-Score:-2.91|P-value:5.46E-4 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:3.44|P-value:8.22E-5 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:-3.67|P-value:2.01E-5 ID:SGTC_490|Compound:phenamil|FD-Score:3.53|P-value:5.35E-5 ID:SGTC_539|Compound:4859-0462|FD-Score:3.05|P-value:4.21E-4 ID:SGTC_550|Compound:4123-0385|FD-Score:5.10|P-value:9.96E-9 ID:SGTC_560|Compound:2332-1943|FD-Score:2.95|P-value:6.26E-4 ID:SGTC_641|Compound:4092-0595|FD-Score:-3.29|P-value:1.16E-4 ID:SGTC_702|Compound:1488-0190|FD-Score:3.83|P-value:1.30E-5 ID:SGTC_782|Compound:0083-0095|FD-Score:-2.87|P-value:6.34E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:10.00|P-value:9.04E-29 ID:SGTC_978|Compound:1416-0512|FD-Score:2.86|P-value:8.72E-4 ID:SGTC_1117|Compound:retinoic acid|FD-Score:3.12|P-value:3.22E-4 ID:SGTC_1186|Compound:1319-0216|FD-Score:-3.38|P-value:7.63E-5 ID:SGTC_1252|Compound:0493-0875|FD-Score:-3.05|P-value:3.20E-4 ID:SGTC_1266|Compound:0828-0227|FD-Score:3.10|P-value:3.40E-4 ID:SGTC_1425|Compound:4048-4433|FD-Score:7.28|P-value:5.06E-16 ID:SGTC_1455|Compound:4262-0120|FD-Score:3.17|P-value:2.58E-4 ID:SGTC_1590|Compound:st077108|FD-Score:3.10|P-value:3.49E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:3.72|P-value:2.30E-5 ID:SGTC_1889|Compound:st059080|FD-Score:4.81|P-value:6.06E-8 ID:SGTC_1897|Compound:st060201|FD-Score:5.23|P-value:4.52E-9 ID:SGTC_1900|Compound:st060200|FD-Score:3.39|P-value:1.03E-4 ID:SGTC_1936|Compound:st074407|FD-Score:5.91|P-value:3.99E-11 ID:SGTC_1942|Compound:st076330|FD-Score:2.88|P-value:8.15E-4 ID:SGTC_1953|Compound:st076800|FD-Score:-3.42|P-value:6.53E-5 ID:SGTC_2025|Compound:5147754|FD-Score:-2.76|P-value:9.56E-4 ID:SGTC_2027|Compound:5150920|FD-Score:-3.03|P-value:3.46E-4 ID:SGTC_2115|Compound:tiliquinol|FD-Score:-3.85|P-value:8.21E-6 ID:SGTC_2259|Compound:7964009|FD-Score:2.91|P-value:7.40E-4 ID:SGTC_2283|Compound:7944793|FD-Score:-2.98|P-value:4.19E-4 ID:SGTC_2296|Compound:7971656|FD-Score:-3.40|P-value:7.01E-5 ID:SGTC_2299|Compound:7976368|FD-Score:3.62|P-value:3.60E-5 ID:SGTC_2301|Compound:7769476|FD-Score:4.65|P-value:1.64E-7 ID:SGTC_2318|Compound:6029776|FD-Score:-2.93|P-value:5.17E-4 ID:SGTC_2351|Compound:9026858|FD-Score:-2.87|P-value:6.39E-4 ID:SGTC_2454|Compound:5-benzylidenerhodanine|FD-Score:2.83|P-value:9.93E-4 ID:SGTC_2535|Compound:4',4'-dimethoxydalbergione|FD-Score:5.71|P-value:1.66E-10 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:3.02|P-value:4.73E-4 ID:SGTC_2610|Compound:ononetin|FD-Score:-3.56|P-value:3.33E-5 ID:SGTC_2613|Compound:3',4'-dimethoxydalbergione|FD-Score:3.08|P-value:3.83E-4 ID:SGTC_2677|Compound:mitomycin C|FD-Score:4.74|P-value:9.35E-8 ID:SGTC_2692|Compound:st077426|FD-Score:2.83|P-value:9.79E-4 ID:SGTC_2858|Compound:9025781|FD-Score:-2.90|P-value:5.69E-4 ID:SGTC_3233|Compound:9132340|FD-Score:2.95|P-value:6.23E-4 ID:SGTC_3254|Compound:9136651|FD-Score:2.85|P-value:9.13E-4 ID:SGTC_3328|Compound:9140624|FD-Score:4.04|P-value:4.67E-6

Top fitness defect scores for YDR092W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_267 4-Nitroquinoline-1-oxide (4-NQO) 16.70 44.4 nM 9.72E-77 DNA damage response 6 3.21E-63
2 SGTC_915 methyl methanesulfonate (MMS) 10.00 110.0 mM 9.04E-29 DNA damage response 2 5.67E-24
3 SGTC_248 streptozotocin 8.07 5.2 mM 2.91E-19 DNA damage response 6 3.57E-16
4 SGTC_1425 4048-4433 7.28 7.3 µM 5.06E-16 copper-dependent oxidative stress 1 1.64E-13
5 SGTC_212 3807-4682 7.25 38.8 µM 6.54E-16 tubulin folding & SWR complex 16 2.02E-13
6 SGTC_1936 st074407 5.91 74.3 µM 3.99E-11 DNA damage response 6 1.75E-9
7 SGTC_2535 4',4'-dimethoxydalbergione 5.71 51.2 µM 1.66E-10 DNA damage response 5 5.66E-9
8 SGTC_1897 st060201 5.23 15.0 µM 4.52E-9 mitochondrial processes 9 8.64E-8
9 SGTC_310 1273-0058 5.15 1.1 µM 7.62E-9 mitochondrial stress 177 1.33E-7
10 SGTC_550 4123-0385 5.10 25.7 µM 9.96E-9 TSC3-RPN4 8 1.66E-7
11 SGTC_214 4373-3496 5.05 70.0 µM 1.38E-8 26 2.17E-7
12 SGTC_1889 st059080 4.81 5.2 µM 6.06E-8 6 7.37E-7
13 SGTC_2677 mitomycin C 4.74 100.0 µM 9.35E-8 DNA damage response 10 1.05E-6
14 SGTC_2301 7769476 4.65 200.0 µM 1.64E-7 16 1.68E-6
15 SGTC_168 3970-0862 4.38 530.5 µM 7.70E-7 95 6.04E-6
16 SGTC_429 1112-0049 4.19 25.5 µM 2.16E-6 DNA damage response 30 1.42E-5
17 SGTC_387 norethindrone acetate 4.06 66.7 µM 4.10E-6 12 2.41E-5
18 SGTC_3328 9140624 4.04 29.0 µM 4.67E-6 19 2.69E-5
19 SGTC_391 gliotoxin 3.88 8.3 µM 1.02E-5 65 5.13E-5
20 SGTC_702 1488-0190 3.83 94.0 µM 1.30E-5 amide catabolism 17 6.30E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.548 1.55E-262 YGL087C MMS2 hom Ubiquitin-conjugating enzyme variant; involved in error-free postreplication repair; forms a heteromeric complex with Ubc13p, an active ubiquitin-conjugating enzyme; cooperates with chromatin-associated RING finger proteins, Rad18p and Rad5p; protein abundance increases in response to DNA replication stress
0.442 1.69E-160 YML095C RAD10 hom Single-stranded DNA endonuclease (with Rad1p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human ERCC1 protein
0.435 2.21E-155 YMR201C RAD14 hom Protein that recognizes and binds damaged DNA during nucleotide excision repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); contains zinc finger motif; homolog of human XPA protein
0.410 2.52E-136 YGR258C RAD2 hom Single-stranded DNA endonuclease, cleaves single-stranded DNA during nucleotide excision repair to excise damaged DNA; subunit of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPG protein
0.399 2.28E-128 YLR032W RAD5 hom DNA helicase; proposed to promote replication fork regression during postreplication repair by template switching; RING finger containing ubiquitin ligase; stimulates the synthesis of free and PCNA-bound polyubiquitin chains by Ubc13p-Mms2p; required for error-prone translesion synthesis; forms nuclear foci upon DNA replication stress
0.365 3.24E-106 YPL022W RAD1 hom Single-stranded DNA endonuclease (with Rad10p), cleaves single-stranded DNA during nucleotide excision repair and double-strand break repair; subunit of Nucleotide Excision Repair Factor 1 (NEF1); homolog of human XPF protein
0.329 1.04E-85 YER162C RAD4 hom Protein that recognizes and binds damaged DNA (with Rad23p) during nucleotide excision repair; subunit of Nuclear Excision Repair Factor 2 (NEF2); also involved, with Rad23p, in turnover of ubiquitylated proteins
0.286 4.58E-64 YCR066W RAD18 hom E3 ubiquitin ligase; forms heterodimer with Rad6p to monoubiquitinate PCNA-K164; heterodimer binds single-stranded DNA and has single-stranded DNA dependent ATPase activity; required for postreplication repair; SUMO-targeted ubiquitin ligase (STUbl) that contains a SUMO-interacting motif (SIM) which stimulates its ubiquitin ligase activity towards the sumoylated form of PCNA
0.266 3.60E-55 YIR002C MPH1 hom 3'-5' DNA helicase involved in error-free bypass of DNA lesions; binds to flap DNA in an error-free bypass pathway and stimulates the activity of Rad27p and Dna2p; also involved in interstrand cross-link repair mutations confer a mutator phenotype; similarity to FANCM, a human Fanconi anemia complementation group protein that along with the MHF complex is involved in stabilizing and remodeling blocked replication forks; member of the SF2 DExD/H superfamily of helicases
0.238 1.40E-44 YBR098W MMS4 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.230 1.20E-41 YDR076W RAD55 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
0.229 4.92E-41 YEL037C RAD23 hom Protein with ubiquitin-like N terminus, subunit of Nuclear Excision Repair Factor 2 (NEF2) with Rad4p that binds damaged DNA; enhances protein deglycosylation activity of Png1p; also involved, with Rad4p, in ubiquitylated protein turnover
0.209 2.20E-34 YDR386W MUS81 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.199 3.36E-31 YDR004W RAD57 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
0.177 5.98E-25 YLR376C PSY3 hom Component of the Shu complex, which promotes error-free DNA repair; Shu complex mediates inhibition of Srs2p function; structural analysis reveals a similar DNA-binding region in both Psy3p and Csm2p and that both regions work together to form a single DNA binding site; deletion of PSY3 results in a mutator phenotype; deletion increases sensitivity to anticancer drugs oxaliplatin and cisplatin but not mitomycin C