YDR096W / GIS1

Histone demethylase and transcription factor; regulates genes during nutrient limitation; negatively regulates DPP1, PHR1; activity modulated by limited proteasome-mediated proteolysis; has JmjC and JmjN domain in N-terminal region that interact, promoting stability and proper transcriptional activity; contains two transactivating domains downstream of Jmj domains and a C-terminal DNA binding domain; GIS1 has a paralog, RPH1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR096W / GIS1

Click on Significant Values for Screen Details ID:SGTC_317|Compound:4495-0002|FD-Score:3.21|P-value:4.41E-4 ID:SGTC_497|Compound:capsazepine|FD-Score:3.05|P-value:7.84E-4 ID:SGTC_502|Compound:l-744,832|FD-Score:3.01|P-value:9.32E-4 ID:SGTC_506|Compound:n9-isopropylolomoucine|FD-Score:3.61|P-value:9.40E-5 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:4.29|P-value:4.50E-6 ID:SGTC_1231|Compound:0242-0792|FD-Score:-3.18|P-value:4.89E-4 ID:SGTC_1312|Compound:1218-2183|FD-Score:3.90|P-value:2.68E-5 ID:SGTC_1372|Compound:2752-0194|FD-Score:-3.03|P-value:8.49E-4 ID:SGTC_1376|Compound:2910-0861|FD-Score:3.37|P-value:2.40E-4 ID:SGTC_1380|Compound:2291-0730|FD-Score:-3.43|P-value:1.89E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-5.03|P-value:9.79E-8 ID:SGTC_1463|Compound:k081-0032|FD-Score:-4.26|P-value:5.25E-6 ID:SGTC_1546|Compound:5-methoxyflavanone|FD-Score:3.87|P-value:3.07E-5 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:-3.49|P-value:1.49E-4 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:3.27|P-value:3.55E-4 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:3.14|P-value:5.65E-4 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:3.23|P-value:4.20E-4 ID:SGTC_1587|Compound:estradiol valerate|FD-Score:4.26|P-value:5.21E-6 ID:SGTC_2143|Compound:5623677|FD-Score:3.29|P-value:3.24E-4 ID:SGTC_2217|Compound:6934799|FD-Score:4.37|P-value:3.00E-6 ID:SGTC_2419|Compound:paclitaxel|FD-Score:3.63|P-value:8.49E-5 ID:SGTC_2458|Compound:5808907|FD-Score:3.04|P-value:8.26E-4 ID:SGTC_2473|Compound:5854593|FD-Score:-4.00|P-value:1.72E-5 ID:SGTC_2537|Compound:larixol|FD-Score:3.98|P-value:1.90E-5 ID:SGTC_2693|Compound:st077581|FD-Score:3.16|P-value:5.31E-4 ID:SGTC_2849|Compound:9019321|FD-Score:3.78|P-value:4.57E-5 ID:SGTC_2859|Compound:9028643|FD-Score:-3.02|P-value:8.97E-4 ID:SGTC_2876|Compound:9059903|FD-Score:3.97|P-value:1.95E-5 ID:SGTC_2909|Compound:7941780|FD-Score:3.52|P-value:1.33E-4 ID:SGTC_2978|Compound:9011784|FD-Score:3.58|P-value:1.05E-4 ID:SGTC_3098|Compound:9119039|FD-Score:-4.19|P-value:7.03E-6 ID:SGTC_317|Compound:4495-0002|FD-Score:3.21|P-value:4.41E-4 ID:SGTC_497|Compound:capsazepine|FD-Score:3.05|P-value:7.84E-4 ID:SGTC_502|Compound:l-744,832|FD-Score:3.01|P-value:9.32E-4 ID:SGTC_506|Compound:n9-isopropylolomoucine|FD-Score:3.61|P-value:9.40E-5 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:4.29|P-value:4.50E-6 ID:SGTC_1231|Compound:0242-0792|FD-Score:-3.18|P-value:4.89E-4 ID:SGTC_1312|Compound:1218-2183|FD-Score:3.90|P-value:2.68E-5 ID:SGTC_1372|Compound:2752-0194|FD-Score:-3.03|P-value:8.49E-4 ID:SGTC_1376|Compound:2910-0861|FD-Score:3.37|P-value:2.40E-4 ID:SGTC_1380|Compound:2291-0730|FD-Score:-3.43|P-value:1.89E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-5.03|P-value:9.79E-8 ID:SGTC_1463|Compound:k081-0032|FD-Score:-4.26|P-value:5.25E-6 ID:SGTC_1546|Compound:5-methoxyflavanone|FD-Score:3.87|P-value:3.07E-5 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:-3.49|P-value:1.49E-4 ID:SGTC_1566|Compound:6,7-dimethoxy-4-ethylcoumarin|FD-Score:3.27|P-value:3.55E-4 ID:SGTC_1572|Compound:epiandrosterone|FD-Score:3.14|P-value:5.65E-4 ID:SGTC_1585|Compound:2',4'-dihydroxychalcone|FD-Score:3.23|P-value:4.20E-4 ID:SGTC_1587|Compound:estradiol valerate|FD-Score:4.26|P-value:5.21E-6 ID:SGTC_2143|Compound:5623677|FD-Score:3.29|P-value:3.24E-4 ID:SGTC_2217|Compound:6934799|FD-Score:4.37|P-value:3.00E-6 ID:SGTC_2419|Compound:paclitaxel|FD-Score:3.63|P-value:8.49E-5 ID:SGTC_2458|Compound:5808907|FD-Score:3.04|P-value:8.26E-4 ID:SGTC_2473|Compound:5854593|FD-Score:-4.00|P-value:1.72E-5 ID:SGTC_2537|Compound:larixol|FD-Score:3.98|P-value:1.90E-5 ID:SGTC_2693|Compound:st077581|FD-Score:3.16|P-value:5.31E-4 ID:SGTC_2849|Compound:9019321|FD-Score:3.78|P-value:4.57E-5 ID:SGTC_2859|Compound:9028643|FD-Score:-3.02|P-value:8.97E-4 ID:SGTC_2876|Compound:9059903|FD-Score:3.97|P-value:1.95E-5 ID:SGTC_2909|Compound:7941780|FD-Score:3.52|P-value:1.33E-4 ID:SGTC_2978|Compound:9011784|FD-Score:3.58|P-value:1.05E-4 ID:SGTC_3098|Compound:9119039|FD-Score:-4.19|P-value:7.03E-6

Top fitness defect scores for YDR096W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2217 6934799 4.37 96.1 µM 3.00E-6 TSC3-RPN4 4 6.15E-6
2 SGTC_1083 didemethylchlorpromazine sulfoxide 4.29 707.4 nM 4.50E-6 redox potentiating 61 8.99E-6
3 SGTC_1587 estradiol valerate 4.26 56.1 µM 5.21E-6 7 1.03E-5
4 SGTC_2537 larixol 3.98 68.9 µM 1.90E-5 21 3.46E-5
5 SGTC_2876 9059903 3.97 26.0 µM 1.95E-5 13 3.54E-5
6 SGTC_1312 1218-2183 3.90 165.0 µM 2.68E-5 9 4.78E-5
7 SGTC_1546 5-methoxyflavanone 3.87 78.7 µM 3.07E-5 11 5.42E-5
8 SGTC_2849 9019321 3.78 13.0 µM 4.57E-5 36 7.87E-5
9 SGTC_2419 paclitaxel 3.63 166.5 µM 8.49E-5 56 1.41E-4
10 SGTC_506 n9-isopropylolomoucine 3.61 153.0 µM 9.40E-5 15 1.55E-4
11 SGTC_2978 9011784 3.58 43.0 µM 1.05E-4 44 1.72E-4
12 SGTC_2909 7941780 3.52 10.5 µM 1.33E-4 31 2.14E-4
13 SGTC_1376 2910-0861 3.37 41.3 µM 2.40E-4 39 3.73E-4
14 SGTC_2143 5623677 3.29 140.5 µM 3.24E-4 16 4.94E-4
15 SGTC_1566 6,7-dimethoxy-4-ethylcoumarin 3.27 85.4 µM 3.55E-4 44 5.38E-4
16 SGTC_1585 2',4'-dihydroxychalcone 3.23 10.4 µM 4.20E-4 64 6.29E-4
17 SGTC_317 4495-0002 3.21 170.0 µM 4.41E-4 30 6.58E-4
18 SGTC_2693 st077581 3.16 40.7 µM 5.31E-4 78 7.83E-4
19 SGTC_1572 epiandrosterone 3.14 68.9 µM 5.65E-4 54 8.31E-4
20 SGTC_497 capsazepine 3.05 133.0 µM 7.84E-4 42 0.00113

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.242 7.57E-46 YPR180W AOS1 het Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Uba2p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
0.236 7.37E-44 YJL171C YJL171C hom GPI-anchored cell wall protein of unknown function; induced in response to cell wall damaging agents and by mutations in genes involved in cell wall biogenesis; sequence similarity to YBR162C/TOS1, a covalently bound cell wall protein; protein abundance increases in response to DNA replication stress
0.235 1.74E-43 YJR146W_p YJR146W_p hom Protein of unknown function; expressed at both mRNA and protein levels; partially overlaps HMS2
0.234 7.68E-43 YGR022C_d YGR022C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps almost completely with the verified ORF MTL1/YGR023W
0.224 2.02E-39 YDR528W HLR1 hom Protein involved in regulation of cell wall composition and integrity and response to osmotic stress; overproduction suppresses a lysis sensitive PKC mutation; similar to Lre1p, which functions antagonistically to protein kinase A;
HLR1 has a paralog, LRE1, that arose from the whole genome duplication
0.223 4.57E-39 YNL166C BNI5 hom Protein involved in organization of septins at the mother-bud neck, may interact directly with the Cdc11p septin, localizes to bud neck in a septin-dependent manner
0.223 6.30E-39 YMR106C YKU80 hom Subunit of the telomeric Ku complex (Yku70p-Yku80p), involved in telomere length maintenance, structure and telomere position effect; relocates to sites of double-strand cleavage to promote nonhomologous end joining during DSB repair
0.213 1.47E-35 YDR521W_d YDR521W_d hom Dubious ORF that overlaps YDR520C; mutant increases expression of PIS1 and RPL3 in glycerol
0.207 8.55E-34 YKL030W_d YKL030W_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; partially overlaps the verified gene MAE1
0.204 5.74E-33 YPL251W_d YPL251W_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C
0.202 2.61E-32 YGR077C PEX8 hom Intraperoxisomal organizer of the peroxisomal import machinery; organizes the formation of the importomer complex, bridging the docking complex with the RING finger complex; tightly associated with the lumenal face of the peroxisomal membrane; essential for peroxisome biogenesis; binds PTS1-signal receptor Pex5p, and PTS2-signal receptor Pex7p
0.201 8.91E-32 YBR274W CHK1 hom Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase
0.198 6.41E-31 YOR094W ARF3 hom Glucose-repressible ADP-ribosylation factor, GTPase of the Ras superfamily involved in development of polarity; also has mRNA binding activity
0.197 7.66E-31 YDL157C_p YDL157C_p hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.193 1.25E-29 YOR152C_p YOR152C_p hom Putative protein of unknown function; YOR152C is not an essential gene