YDR099W / BMH2

14-3-3 protein, minor isoform; controls proteome at post-transcriptional level, binds proteins and DNA, involved in regulation of many processes including exocytosis, vesicle transport, Ras/MAPK signaling, and rapamycin-sensitive signaling; protein increases in abundance and relative distribution to the nucleus increases upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR099W / BMH2

Click on Significant Values for Screen Details ID:SGTC_353|Compound:1988-1175|FD-Score:-3.50|P-value:3.40E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:5.09|P-value:4.46E-7 ID:SGTC_494|Compound:Boc-Gly-Val-Val-CHO|FD-Score:-3.56|P-value:2.73E-4 ID:SGTC_497|Compound:capsazepine|FD-Score:-3.77|P-value:1.25E-4 ID:SGTC_503|Compound:tyrphostin 8|FD-Score:3.96|P-value:6.73E-5 ID:SGTC_511|Compound:blebbistatin|FD-Score:5.48|P-value:6.33E-8 ID:SGTC_555|Compound:4296-0685|FD-Score:4.32|P-value:1.55E-5 ID:SGTC_558|Compound:k064-0027|FD-Score:-4.35|P-value:1.20E-5 ID:SGTC_561|Compound:1550-0330|FD-Score:-4.09|P-value:3.62E-5 ID:SGTC_701|Compound:4424-0219|FD-Score:3.23|P-value:9.33E-4 ID:SGTC_771|Compound:0250-0039|FD-Score:4.57|P-value:5.17E-6 ID:SGTC_842|Compound:0271-0008|FD-Score:3.43|P-value:4.83E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:-4.16|P-value:2.76E-5 ID:SGTC_947|Compound:3447-0010|FD-Score:3.62|P-value:2.43E-4 ID:SGTC_998|Compound:3996-0085|FD-Score:-4.61|P-value:3.78E-6 ID:SGTC_1004|Compound:4084-0029|FD-Score:-3.18|P-value:9.96E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-3.99|P-value:5.28E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-5.80|P-value:9.33E-9 ID:SGTC_1415|Compound:3966-0071|FD-Score:-3.20|P-value:9.35E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.44|P-value:4.21E-4 ID:SGTC_2165|Compound:5727197|FD-Score:3.25|P-value:8.80E-4 ID:SGTC_2287|Compound:7945887|FD-Score:3.34|P-value:6.56E-4 ID:SGTC_2322|Compound:7413319|FD-Score:4.59|P-value:4.67E-6 ID:SGTC_2326|Compound:6164455|FD-Score:4.85|P-value:1.47E-6 ID:SGTC_2364|Compound:9055074|FD-Score:3.63|P-value:2.36E-4 ID:SGTC_2543|Compound:pimpinellin|FD-Score:-3.39|P-value:4.93E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:-3.39|P-value:5.02E-4 ID:SGTC_2709|Compound:st078560|FD-Score:3.47|P-value:4.19E-4 ID:SGTC_2859|Compound:9028643|FD-Score:-4.03|P-value:4.58E-5 ID:SGTC_2934|Compound:9036370|FD-Score:3.73|P-value:1.62E-4 ID:SGTC_2987|Compound:9018338|FD-Score:5.92|P-value:5.65E-9 ID:SGTC_3040|Compound:9091894|FD-Score:-3.34|P-value:5.99E-4 ID:SGTC_353|Compound:1988-1175|FD-Score:-3.50|P-value:3.40E-4 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:5.09|P-value:4.46E-7 ID:SGTC_494|Compound:Boc-Gly-Val-Val-CHO|FD-Score:-3.56|P-value:2.73E-4 ID:SGTC_497|Compound:capsazepine|FD-Score:-3.77|P-value:1.25E-4 ID:SGTC_503|Compound:tyrphostin 8|FD-Score:3.96|P-value:6.73E-5 ID:SGTC_511|Compound:blebbistatin|FD-Score:5.48|P-value:6.33E-8 ID:SGTC_555|Compound:4296-0685|FD-Score:4.32|P-value:1.55E-5 ID:SGTC_558|Compound:k064-0027|FD-Score:-4.35|P-value:1.20E-5 ID:SGTC_561|Compound:1550-0330|FD-Score:-4.09|P-value:3.62E-5 ID:SGTC_701|Compound:4424-0219|FD-Score:3.23|P-value:9.33E-4 ID:SGTC_771|Compound:0250-0039|FD-Score:4.57|P-value:5.17E-6 ID:SGTC_842|Compound:0271-0008|FD-Score:3.43|P-value:4.83E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:-4.16|P-value:2.76E-5 ID:SGTC_947|Compound:3447-0010|FD-Score:3.62|P-value:2.43E-4 ID:SGTC_998|Compound:3996-0085|FD-Score:-4.61|P-value:3.78E-6 ID:SGTC_1004|Compound:4084-0029|FD-Score:-3.18|P-value:9.96E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:-3.99|P-value:5.28E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-5.80|P-value:9.33E-9 ID:SGTC_1415|Compound:3966-0071|FD-Score:-3.20|P-value:9.35E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.44|P-value:4.21E-4 ID:SGTC_2165|Compound:5727197|FD-Score:3.25|P-value:8.80E-4 ID:SGTC_2287|Compound:7945887|FD-Score:3.34|P-value:6.56E-4 ID:SGTC_2322|Compound:7413319|FD-Score:4.59|P-value:4.67E-6 ID:SGTC_2326|Compound:6164455|FD-Score:4.85|P-value:1.47E-6 ID:SGTC_2364|Compound:9055074|FD-Score:3.63|P-value:2.36E-4 ID:SGTC_2543|Compound:pimpinellin|FD-Score:-3.39|P-value:4.93E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:-3.39|P-value:5.02E-4 ID:SGTC_2709|Compound:st078560|FD-Score:3.47|P-value:4.19E-4 ID:SGTC_2859|Compound:9028643|FD-Score:-4.03|P-value:4.58E-5 ID:SGTC_2934|Compound:9036370|FD-Score:3.73|P-value:1.62E-4 ID:SGTC_2987|Compound:9018338|FD-Score:5.92|P-value:5.65E-9 ID:SGTC_3040|Compound:9091894|FD-Score:-3.34|P-value:5.99E-4

Top fitness defect scores for YDR099W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2987 9018338 5.92 71.4 µM 5.65E-9 RPP1 & pyrimidine depletion 3 1.65E-9
2 SGTC_511 blebbistatin 5.48 171.0 µM 6.33E-8 DNA intercalators 6 2.18E-8
3 SGTC_457 grayanotoxin iii 5.09 135.0 µM 4.46E-7 27 1.76E-7
4 SGTC_2326 6164455 4.85 177.6 µM 1.47E-6 mitochondrial processes 7 6.30E-7
5 SGTC_2322 7413319 4.59 38.2 µM 4.67E-6 ERAD & cell cycle 119 2.17E-6
6 SGTC_771 0250-0039 4.57 27.3 µM 5.17E-6 7 2.41E-6
7 SGTC_555 4296-0685 4.32 37.0 µM 1.55E-5 ergosterol biosynthesis 5 7.80E-6
8 SGTC_503 tyrphostin 8 3.96 294.0 µM 6.73E-5 TSC3-RPN4 25 3.75E-5
9 SGTC_2934 9036370 3.73 36.9 µM 1.62E-4 40 9.54E-5
10 SGTC_2364 9055074 3.63 200.0 µM 2.36E-4 27 1.43E-4
11 SGTC_947 3447-0010 3.62 211.0 µM 2.43E-4 22 1.48E-4
12 SGTC_2709 st078560 3.47 56.0 µM 4.19E-4 22 2.64E-4
13 SGTC_842 0271-0008 3.43 11.1 µM 4.83E-4 heme biosynthesis & mitochondrial translocase 34 3.07E-4
14 SGTC_2287 7945887 3.34 168.8 µM 6.56E-4 15 4.26E-4
15 SGTC_2165 5727197 3.25 32.1 µM 8.80E-4 65 5.83E-4
16 SGTC_701 4424-0219 3.23 185.0 µM 9.33E-4 calcium & mitochondrial duress 38 6.21E-4
17 SGTC_1918 oxolan-2-ylmethyl 3-bromobenzoate 3.20 70.1 µM 0.00104 67 6.97E-4
18 SGTC_2621 lithochol-11-enic acid 3.11 100.0 µM 0.00138 71 9.44E-4
19 SGTC_3165 9101544 3.09 49.5 µM 0.00146 33 9.99E-4
20 SGTC_1412 3909-8563 3.07 178.0 µM 0.00155 39 0.00107

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.199 2.56E-31 YGL081W_p YGL081W_p hom Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis
0.180 9.26E-26 YBL036C YBL036C hom Putative non-specific single-domain racemase based on structural similarity; binds pyridoxal 5'-phosphate; expression of GFP-fusion protein induced in response to the DNA-damaging agent MMS
0.175 1.29E-24 YJL135W_d YJL135W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified genes YJL134W/LCB3
0.171 2.14E-23 YJR019C TES1 hom Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids
0.165 5.46E-22 YBL086C_p YBL086C_p hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.163 1.76E-21 YML084W_d YML084W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.157 7.83E-20 YDL110C TMA17 hom Protein of unknown function that associates with ribosomes; heterozygous deletion demonstrated increases in chromosome instability in a rad9 deletion background; protein abundance is decreased upon intracellular iron depletion
0.150 3.05E-18 YLR205C HMX1 hom ER localized heme oxygenase, involved in heme degradation during iron starvation and in the oxidative stress response; expression is regulated by AFT1 and oxidative stress; relocates to the perinuclear region in the presence of oxidants
0.146 1.66E-17 YDR122W KIN1 hom Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p
0.146 1.84E-17 YPL264C_p YPL264C_p hom Putative membrane protein of unknown function; physically interacts with Hsp82p; YPL264C is not an essential gene
0.145 3.71E-17 YGR025W_d YGR025W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.138 9.89E-16 YOR012W_p YOR012W_p hom Putative protein of unknown function
0.136 2.74E-15 YKL205W LOS1 hom Nuclear pore protein involved in nuclear export of pre-tRNA and in re-export of mature tRNAs after their retrograde import from the cytoplasm
0.134 5.58E-15 YLR016C PML1 hom Subunit of the RES complex, which is required for nuclear retention of unspliced pre-mRNAs; acts in the same pathway as Pml39p and Mlp1p
0.132 1.39E-14 YOR238W_p YOR238W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm