YDR105C / TMS1

Vacuolar membrane protein of unknown function that is conserved in mammals; predicted to contain eleven transmembrane helices; interacts with Pdr5p, a protein involved in multidrug resistance

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR105C / TMS1

Click on Significant Values for Screen Details ID:SGTC_266|Compound:formosanin C|FD-Score:-3.19|P-value:9.15E-4 ID:SGTC_288|Compound:1315-0019|FD-Score:3.41|P-value:4.60E-4 ID:SGTC_382|Compound:3229-0966|FD-Score:-3.92|P-value:6.49E-5 ID:SGTC_530|Compound:4358-1714|FD-Score:-4.04|P-value:4.04E-5 ID:SGTC_647|Compound:0140-0236|FD-Score:3.69|P-value:1.68E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:3.32|P-value:6.37E-4 ID:SGTC_962|Compound:1254-0300|FD-Score:3.79|P-value:1.14E-4 ID:SGTC_1023|Compound:4482-3888|FD-Score:3.44|P-value:4.19E-4 ID:SGTC_1178|Compound:0988-0033|FD-Score:3.54|P-value:2.94E-4 ID:SGTC_1187|Compound:1391-0724|FD-Score:3.94|P-value:6.31E-5 ID:SGTC_1196|Compound:1588-0294|FD-Score:-3.64|P-value:1.87E-4 ID:SGTC_1283|Compound:0922-0125|FD-Score:3.70|P-value:1.63E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:3.46|P-value:3.82E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:3.86|P-value:8.67E-5 ID:SGTC_1712|Compound:st032288|FD-Score:3.83|P-value:9.84E-5 ID:SGTC_2264|Compound:7948757|FD-Score:3.41|P-value:4.54E-4 ID:SGTC_2305|Compound:7443522|FD-Score:-3.52|P-value:2.95E-4 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:4.73|P-value:2.16E-6 ID:SGTC_2759|Compound:disulfiram|FD-Score:5.19|P-value:2.22E-7 ID:SGTC_2760|Compound:etidronate|FD-Score:6.65|P-value:4.75E-11 ID:SGTC_2762|Compound:leflunomide|FD-Score:4.48|P-value:6.74E-6 ID:SGTC_2937|Compound:9044753|FD-Score:3.41|P-value:4.52E-4 ID:SGTC_2990|Compound:9074105|FD-Score:3.21|P-value:9.04E-4 ID:SGTC_3005|Compound:9076496|FD-Score:3.61|P-value:2.23E-4 ID:SGTC_3210|Compound:9128157|FD-Score:6.52|P-value:1.11E-10 ID:SGTC_3356|Compound:9151811|FD-Score:3.35|P-value:5.69E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:-3.19|P-value:9.15E-4 ID:SGTC_288|Compound:1315-0019|FD-Score:3.41|P-value:4.60E-4 ID:SGTC_382|Compound:3229-0966|FD-Score:-3.92|P-value:6.49E-5 ID:SGTC_530|Compound:4358-1714|FD-Score:-4.04|P-value:4.04E-5 ID:SGTC_647|Compound:0140-0236|FD-Score:3.69|P-value:1.68E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:3.32|P-value:6.37E-4 ID:SGTC_962|Compound:1254-0300|FD-Score:3.79|P-value:1.14E-4 ID:SGTC_1023|Compound:4482-3888|FD-Score:3.44|P-value:4.19E-4 ID:SGTC_1178|Compound:0988-0033|FD-Score:3.54|P-value:2.94E-4 ID:SGTC_1187|Compound:1391-0724|FD-Score:3.94|P-value:6.31E-5 ID:SGTC_1196|Compound:1588-0294|FD-Score:-3.64|P-value:1.87E-4 ID:SGTC_1283|Compound:0922-0125|FD-Score:3.70|P-value:1.63E-4 ID:SGTC_1335|Compound:1486-0900|FD-Score:3.46|P-value:3.82E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:3.86|P-value:8.67E-5 ID:SGTC_1712|Compound:st032288|FD-Score:3.83|P-value:9.84E-5 ID:SGTC_2264|Compound:7948757|FD-Score:3.41|P-value:4.54E-4 ID:SGTC_2305|Compound:7443522|FD-Score:-3.52|P-value:2.95E-4 ID:SGTC_2758|Compound:dehydroepiandrosterone|FD-Score:4.73|P-value:2.16E-6 ID:SGTC_2759|Compound:disulfiram|FD-Score:5.19|P-value:2.22E-7 ID:SGTC_2760|Compound:etidronate|FD-Score:6.65|P-value:4.75E-11 ID:SGTC_2762|Compound:leflunomide|FD-Score:4.48|P-value:6.74E-6 ID:SGTC_2937|Compound:9044753|FD-Score:3.41|P-value:4.52E-4 ID:SGTC_2990|Compound:9074105|FD-Score:3.21|P-value:9.04E-4 ID:SGTC_3005|Compound:9076496|FD-Score:3.61|P-value:2.23E-4 ID:SGTC_3210|Compound:9128157|FD-Score:6.52|P-value:1.11E-10 ID:SGTC_3356|Compound:9151811|FD-Score:3.35|P-value:5.69E-4

Top fitness defect scores for YDR105C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2760 etidronate 6.65 98.0 µM 4.75E-11 2 1.43E-11
2 SGTC_3210 9128157 6.52 49.5 µM 1.11E-10 11 3.49E-11
3 SGTC_2759 disulfiram 5.19 3.4 µM 2.22E-7 12 1.05E-7
4 SGTC_2758 dehydroepiandrosterone 4.73 69.3 µM 2.16E-6 24 1.15E-6
5 SGTC_2762 leflunomide 4.48 42.1 µM 6.74E-6 14 3.81E-6
6 SGTC_1187 1391-0724 3.94 117.0 µM 6.31E-5 amide catabolism 17 4.01E-5
7 SGTC_1495 4469-0029 3.86 237.0 µM 8.67E-5 114 5.60E-5
8 SGTC_1712 st032288 3.83 53.4 µM 9.84E-5 calcium & mitochondrial duress 194 6.41E-5
9 SGTC_962 1254-0300 3.79 21.5 µM 1.14E-4 28 7.50E-5
10 SGTC_1283 0922-0125 3.70 16.4 µM 1.63E-4 31 1.09E-4
11 SGTC_647 0140-0236 3.69 81.0 µM 1.68E-4 14 1.13E-4
12 SGTC_3005 9076496 3.61 71.4 µM 2.23E-4 11 1.51E-4
13 SGTC_1178 0988-0033 3.54 191.0 µM 2.94E-4 42 2.03E-4
14 SGTC_1335 1486-0900 3.46 202.0 µM 3.82E-4 46 2.67E-4
15 SGTC_1023 4482-3888 3.44 16.0 µM 4.19E-4 TSC3-RPN4 31 2.94E-4
16 SGTC_2937 9044753 3.41 11.3 µM 4.52E-4 60S ribosome export 56 3.19E-4
17 SGTC_2264 7948757 3.41 110.8 µM 4.54E-4 33 3.20E-4
18 SGTC_288 1315-0019 3.41 35.1 µM 4.60E-4 RSC & ERG11 39 3.25E-4
19 SGTC_3356 9151811 3.35 4.5 µM 5.69E-4 cell wall 39 4.06E-4
20 SGTC_933 2914-0560 3.32 114.0 µM 6.37E-4 47 4.57E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.158 3.46E-20 YDR475C JIP4 hom Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.137 1.29E-15 YOR086C TCB1 hom Lipid-binding ER protein involved in ER-plasma membrane tethering; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PM phosphatidylinositol-4-phosphate (PI4P) levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; contains three calcium and lipid binding domains; non-tagged protein also localizes to mitochondria; C-termini of Tcb1p, Tcb2p and Tcb3p interact
0.127 1.75E-13 YGR040W KSS1 hom Mitogen-activated protein kinase (MAPK) involved in signal transduction pathways that control filamentous growth and pheromone response; the KSS1 gene is nonfunctional in S288C strains and functional in W303 strains
0.126 2.87E-13 YDR262W_p YDR262W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole and is induced in response to the DNA-damaging agent MMS; gene expression increases in response to Zymoliase treatment
0.125 3.87E-13 YKL051W SFK1 hom Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane
0.120 3.58E-12 YDR466W PKH3 hom Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
0.117 8.57E-12 YLL017W YLL017W hom Non-essential Ras guanine nucleotide exchange factor (GEF); localized to the membrane; expressed in poor nutrient conditions and on nonfermentable carbon sources; contains a stop codon in S288C, full-length gene includes YLL016W; SDC25 has a paralog, CDC25, that arose from the whole genome duplication
0.113 4.96E-11 YJL164C TPK1 hom cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication
0.110 1.88E-10 YNL001W DOM34 hom Protein that facilitates ribosomal subunit dissociation; acts when translation is stalled, with binding partner Hbs1p; required for RNA cleavage in no-go decay, but reports conflict on endonuclease activity; Pelota ortholog; protein abundance increases in response to DNA replication stress; DOM34 has a paralog, YCL001W-B, that arose from the whole genome duplication
0.109 2.13E-10 YHL002W HSE1 hom Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes
0.108 3.72E-10 YDL079C MRK1 hom Glycogen synthase kinase 3 (GSK-3) homolog; one of four GSK-3 homologs in S. cerevisiae that function to activate Msn2p-dependent transcription of stress responsive genes and that function in protein degradation
0.105 9.59E-10 YMR004W MVP1 hom Protein required for sorting proteins to the vacuole; Mvp1p and Vps1p act in concert to promote membrane traffic to the vacuole; participates in transcription initiation and/or early elongation of specific genes; interacts with foot domain of RNA polymerase II; deletion results in abnormal CTD-Ser5 phosphorylation of RNA polymerase II at specific promoter regions; protein abundance increases in response to DNA replication stress
0.102 2.87E-9 YJL165C HAL5 hom Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication
0.102 3.49E-9 YBL071W-A KTI11 hom Zn-ribbon protein that co-purifies with Dph1 and Dph2 in a complex required for synthesis of diphthamide on translation factor eEF2 and with Elongator subunits Iki3p, Elp2p, and Elp3p involved in modification of wobble nucleosides in tRNAs
0.102 3.71E-9 YMR008C PLB1 hom Phospholipase B (lysophospholipase) involved in lipid metabolism, required for deacylation of phosphatidylcholine and phosphatidylethanolamine but not phosphatidylinositol