YDR118W / APC4

Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C); APC/C is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; component of the platform domain of the APC/C, based on structural analysis; relative distribution to the nucleus increases upon DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR118W / APC4

Click on Significant Values for Screen Details ID:SGTC_45|Compound:0kpi-0036|FD-Score:5.09|P-value:2.96E-8 ID:SGTC_67|Compound:0335-0847|FD-Score:3.53|P-value:8.57E-5 ID:SGTC_137|Compound:0438-0332|FD-Score:2.98|P-value:7.75E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:4.25|P-value:3.03E-6 ID:SGTC_278|Compound:4092-0821|FD-Score:3.61|P-value:5.96E-5 ID:SGTC_332|Compound:4112-0506|FD-Score:3.60|P-value:6.27E-5 ID:SGTC_367|Compound:0335-0881|FD-Score:5.70|P-value:6.11E-10 ID:SGTC_405|Compound:ebelactone b|FD-Score:-2.93|P-value:8.42E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.09|P-value:5.04E-4 ID:SGTC_496|Compound:calpeptin|FD-Score:-3.03|P-value:5.87E-4 ID:SGTC_541|Compound:4513-1321|FD-Score:3.23|P-value:2.90E-4 ID:SGTC_542|Compound:3381-0244|FD-Score:3.04|P-value:6.21E-4 ID:SGTC_561|Compound:1550-0330|FD-Score:-3.14|P-value:3.91E-4 ID:SGTC_582|Compound:0987-0078|FD-Score:-3.45|P-value:1.11E-4 ID:SGTC_783|Compound:0848-0141|FD-Score:3.02|P-value:6.66E-4 ID:SGTC_820|Compound:1582-0060|FD-Score:3.45|P-value:1.21E-4 ID:SGTC_836|Compound:0330-0104|FD-Score:-3.21|P-value:2.93E-4 ID:SGTC_840|Compound:0245-0196|FD-Score:3.53|P-value:8.47E-5 ID:SGTC_855|Compound:0428-0020|FD-Score:-3.02|P-value:6.12E-4 ID:SGTC_969|Compound:1313-0238|FD-Score:3.01|P-value:6.90E-4 ID:SGTC_981|Compound:1488-1094|FD-Score:-3.13|P-value:4.06E-4 ID:SGTC_1063|Compound:disulfiram|FD-Score:3.88|P-value:1.81E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.69|P-value:3.93E-5 ID:SGTC_1204|Compound:4049-0315|FD-Score:3.53|P-value:8.74E-5 ID:SGTC_1215|Compound:3852-0577|FD-Score:3.35|P-value:1.85E-4 ID:SGTC_1282|Compound:0886-0010|FD-Score:3.03|P-value:6.34E-4 ID:SGTC_1746|Compound:st038117|FD-Score:3.33|P-value:1.94E-4 ID:SGTC_1906|Compound:st060206|FD-Score:3.31|P-value:2.12E-4 ID:SGTC_2076|Compound:5212012|FD-Score:2.93|P-value:9.24E-4 ID:SGTC_2093|Compound:5331342|FD-Score:2.96|P-value:8.33E-4 ID:SGTC_2110|Compound:5483698|FD-Score:3.10|P-value:4.88E-4 ID:SGTC_2189|Compound:6047310|FD-Score:-3.38|P-value:1.46E-4 ID:SGTC_2308|Compound:7584454|FD-Score:3.31|P-value:2.11E-4 ID:SGTC_2317|Compound:5986382|FD-Score:3.00|P-value:6.97E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:-4.67|P-value:2.96E-7 ID:SGTC_2415|Compound:st077333|FD-Score:3.38|P-value:1.58E-4 ID:SGTC_2458|Compound:5808907|FD-Score:4.34|P-value:1.93E-6 ID:SGTC_2459|Compound:5811157|FD-Score:3.16|P-value:3.80E-4 ID:SGTC_2471|Compound:5466956|FD-Score:3.83|P-value:2.29E-5 ID:SGTC_2482|Compound:5981709|FD-Score:5.06|P-value:3.57E-8 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.23|P-value:2.97E-4 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:3.05|P-value:5.84E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:7.93|P-value:1.39E-17 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.05|P-value:7.98E-6 ID:SGTC_2583|Compound:asiatic acid|FD-Score:3.88|P-value:1.83E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:-4.17|P-value:4.03E-6 ID:SGTC_3048|Compound:9093155|FD-Score:3.03|P-value:6.41E-4 ID:SGTC_3066|Compound:9117245|FD-Score:-4.49|P-value:7.65E-7 ID:SGTC_3200|Compound:9114385|FD-Score:-3.39|P-value:1.45E-4 ID:SGTC_3210|Compound:9128157|FD-Score:-3.34|P-value:1.76E-4 ID:SGTC_45|Compound:0kpi-0036|FD-Score:5.09|P-value:2.96E-8 ID:SGTC_67|Compound:0335-0847|FD-Score:3.53|P-value:8.57E-5 ID:SGTC_137|Compound:0438-0332|FD-Score:2.98|P-value:7.75E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:4.25|P-value:3.03E-6 ID:SGTC_278|Compound:4092-0821|FD-Score:3.61|P-value:5.96E-5 ID:SGTC_332|Compound:4112-0506|FD-Score:3.60|P-value:6.27E-5 ID:SGTC_367|Compound:0335-0881|FD-Score:5.70|P-value:6.11E-10 ID:SGTC_405|Compound:ebelactone b|FD-Score:-2.93|P-value:8.42E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.09|P-value:5.04E-4 ID:SGTC_496|Compound:calpeptin|FD-Score:-3.03|P-value:5.87E-4 ID:SGTC_541|Compound:4513-1321|FD-Score:3.23|P-value:2.90E-4 ID:SGTC_542|Compound:3381-0244|FD-Score:3.04|P-value:6.21E-4 ID:SGTC_561|Compound:1550-0330|FD-Score:-3.14|P-value:3.91E-4 ID:SGTC_582|Compound:0987-0078|FD-Score:-3.45|P-value:1.11E-4 ID:SGTC_783|Compound:0848-0141|FD-Score:3.02|P-value:6.66E-4 ID:SGTC_820|Compound:1582-0060|FD-Score:3.45|P-value:1.21E-4 ID:SGTC_836|Compound:0330-0104|FD-Score:-3.21|P-value:2.93E-4 ID:SGTC_840|Compound:0245-0196|FD-Score:3.53|P-value:8.47E-5 ID:SGTC_855|Compound:0428-0020|FD-Score:-3.02|P-value:6.12E-4 ID:SGTC_969|Compound:1313-0238|FD-Score:3.01|P-value:6.90E-4 ID:SGTC_981|Compound:1488-1094|FD-Score:-3.13|P-value:4.06E-4 ID:SGTC_1063|Compound:disulfiram|FD-Score:3.88|P-value:1.81E-5 ID:SGTC_1107|Compound:amlodipine|FD-Score:-3.69|P-value:3.93E-5 ID:SGTC_1204|Compound:4049-0315|FD-Score:3.53|P-value:8.74E-5 ID:SGTC_1215|Compound:3852-0577|FD-Score:3.35|P-value:1.85E-4 ID:SGTC_1282|Compound:0886-0010|FD-Score:3.03|P-value:6.34E-4 ID:SGTC_1746|Compound:st038117|FD-Score:3.33|P-value:1.94E-4 ID:SGTC_1906|Compound:st060206|FD-Score:3.31|P-value:2.12E-4 ID:SGTC_2076|Compound:5212012|FD-Score:2.93|P-value:9.24E-4 ID:SGTC_2093|Compound:5331342|FD-Score:2.96|P-value:8.33E-4 ID:SGTC_2110|Compound:5483698|FD-Score:3.10|P-value:4.88E-4 ID:SGTC_2189|Compound:6047310|FD-Score:-3.38|P-value:1.46E-4 ID:SGTC_2308|Compound:7584454|FD-Score:3.31|P-value:2.11E-4 ID:SGTC_2317|Compound:5986382|FD-Score:3.00|P-value:6.97E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:-4.67|P-value:2.96E-7 ID:SGTC_2415|Compound:st077333|FD-Score:3.38|P-value:1.58E-4 ID:SGTC_2458|Compound:5808907|FD-Score:4.34|P-value:1.93E-6 ID:SGTC_2459|Compound:5811157|FD-Score:3.16|P-value:3.80E-4 ID:SGTC_2471|Compound:5466956|FD-Score:3.83|P-value:2.29E-5 ID:SGTC_2482|Compound:5981709|FD-Score:5.06|P-value:3.57E-8 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.23|P-value:2.97E-4 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:3.05|P-value:5.84E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:7.93|P-value:1.39E-17 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.05|P-value:7.98E-6 ID:SGTC_2583|Compound:asiatic acid|FD-Score:3.88|P-value:1.83E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:-4.17|P-value:4.03E-6 ID:SGTC_3048|Compound:9093155|FD-Score:3.03|P-value:6.41E-4 ID:SGTC_3066|Compound:9117245|FD-Score:-4.49|P-value:7.65E-7 ID:SGTC_3200|Compound:9114385|FD-Score:-3.39|P-value:1.45E-4 ID:SGTC_3210|Compound:9128157|FD-Score:-3.34|P-value:1.76E-4

Top fitness defect scores for YDR118W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2567 aureobasidin a 7.93 60.0 nM 1.39E-17 17 1.08E-15 2.04
2 SGTC_367 0335-0881 5.70 7.1 µM 6.11E-10 ubiquinone biosynthesis & proteasome 9 5.86E-9 0.25
3 SGTC_45 0kpi-0036 5.09 14.9 µM 2.96E-8 excess fatty acid 4 1.80E-7 0.19
4 SGTC_2482 5981709 5.06 142.7 µM 3.57E-8 PDR1 8 2.12E-7 0.50
5 SGTC_2458 5808907 4.34 16.4 µM 1.93E-6 2 7.22E-6 0.32
6 SGTC_266 formosanin C 4.25 406.0 nM 3.03E-6 sphingolipid biosynthesis & PDR1 22 1.08E-5 0.16
7 SGTC_2582 agaric acid 4.05 100.0 µM 7.98E-6 redox potentiating 31 2.53E-5 0.02
8 SGTC_1063 disulfiram 3.88 1.8 µM 1.81E-5 9 5.22E-5 0.12
9 SGTC_2583 asiatic acid 3.88 100.0 µM 1.83E-5 11 5.28E-5 0.12
10 SGTC_2471 5466956 3.83 24.8 µM 2.29E-5 mitochondrial processes 20 6.44E-5 0.16
11 SGTC_278 4092-0821 3.61 23.9 µM 5.96E-5 plasma membrane duress 4 1.50E-4 0.55
12 SGTC_332 4112-0506 3.60 40.9 µM 6.27E-5 28 1.57E-4 0.01
13 SGTC_840 0245-0196 3.53 390.0 µM 8.47E-5 4 2.05E-4 0.10
14 SGTC_67 0335-0847 3.53 266.0 µM 8.57E-5 ubiquinone biosynthesis & proteasome 27 2.07E-4 0.01
15 SGTC_1204 4049-0315 3.53 18.3 µM 8.74E-5 13 2.11E-4 0.00
16 SGTC_820 1582-0060 3.45 59.9 µM 1.21E-4 14 2.82E-4 0.01
17 SGTC_2415 st077333 3.38 57.7 µM 1.58E-4 22 3.58E-4 0.05
18 SGTC_1215 3852-0577 3.35 11.2 µM 1.85E-4 13 4.10E-4 0.00
19 SGTC_1746 st038117 3.33 42.0 µM 1.94E-4 9 4.28E-4 0.05
20 SGTC_2308 7584454 3.31 197.6 µM 2.11E-4 20 4.62E-4 0.12

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.310 1.66E-75 YKL004W AUR1 het Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
0.207 5.66E-34 YMR211W DML1 het Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
0.192 4.26E-29 YDR367W KEI1 het Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
0.175 2.08E-24 YJL119C_d YJL119C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.162 3.02E-21 YMR102C_p YMR102C_p hom Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene
0.156 1.06E-19 YNL027W CRZ1 hom Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress
0.137 1.29E-15 YDR326C YSP2 hom Protein involved in programmed cell death; mutant shows resistance to cell death induced by amiodarone or intracellular acidification; YSP2 has a paralog, YHR080C, that arose from the whole genome duplication
0.135 3.98E-15 YKL182W FAS1 het Beta subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains acetyltransacylase, dehydratase, enoyl reductase, malonyl transacylase, and palmitoyl transacylase activities
0.135 4.28E-15 YPL224C MMT2 hom Putative metal transporter involved in mitochondrial iron accumulation; closely related to Mmt1p
0.134 8.03E-15 YML081C-A ATP18 hom Subunit of the mitochondrial F1F0 ATP synthase, which is a large enzyme complex required for ATP synthesis; termed subunit I or subunit j; does not correspond to known ATP synthase subunits in other organisms
0.133 1.11E-14 YLR241W_p YLR241W_p hom Putative protein of unknown function, may be involved in detoxification
0.131 2.78E-14 YBR110W ALG1 het Mannosyltransferase, involved in asparagine-linked glycosylation in the endoplasmic reticulum (ER); essential for viability, mutation is functionally complemented by human ortholog
0.129 5.27E-14 YNL257C SIP3 hom Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication
0.128 1.21E-13 YOR050C_d YOR050C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutation is viable
0.127 1.35E-13 YML052W SUR7 hom Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants