YDR119W_p / VBA4

Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR119W / VBA4

Click on Significant Values for Screen Details ID:SGTC_42|Compound:0133-0086|FD-Score:-3.01|P-value:3.08E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:-2.85|P-value:5.94E-4 ID:SGTC_167|Compound:3672-0051|FD-Score:-3.01|P-value:3.16E-4 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:3.28|P-value:8.66E-5 ID:SGTC_249|Compound:wiskostatin|FD-Score:3.53|P-value:2.66E-5 ID:SGTC_375|Compound:3970-0961|FD-Score:-4.46|P-value:1.89E-7 ID:SGTC_380|Compound:3970-0968|FD-Score:-3.03|P-value:2.93E-4 ID:SGTC_383|Compound:2324-0395|FD-Score:3.57|P-value:2.13E-5 ID:SGTC_597|Compound:k018-0003|FD-Score:-2.87|P-value:5.49E-4 ID:SGTC_613|Compound:3914-0030|FD-Score:-3.82|P-value:6.94E-6 ID:SGTC_615|Compound:k087-0205|FD-Score:-3.65|P-value:1.69E-5 ID:SGTC_620|Compound:0109-0052|FD-Score:8.32|P-value:9.38E-22 ID:SGTC_625|Compound:k213-0058|FD-Score:5.67|P-value:4.32E-11 ID:SGTC_628|Compound:1636-0253|FD-Score:6.39|P-value:1.27E-13 ID:SGTC_648|Compound:0375-0473|FD-Score:-4.09|P-value:1.62E-6 ID:SGTC_662|Compound:k832-1615|FD-Score:4.32|P-value:3.80E-7 ID:SGTC_737|Compound:1950-0117|FD-Score:2.97|P-value:3.22E-4 ID:SGTC_749|Compound:1000-0129|FD-Score:3.41|P-value:4.67E-5 ID:SGTC_785|Compound:1270-0111|FD-Score:-4.89|P-value:1.27E-8 ID:SGTC_789|Compound:1309-1135|FD-Score:-3.04|P-value:2.72E-4 ID:SGTC_825|Compound:1988-2224|FD-Score:3.08|P-value:2.04E-4 ID:SGTC_981|Compound:1488-1094|FD-Score:-3.04|P-value:2.72E-4 ID:SGTC_986|Compound:1493-0483|FD-Score:3.50|P-value:3.06E-5 ID:SGTC_1214|Compound:0358-0031|FD-Score:2.91|P-value:4.18E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:2.71|P-value:9.18E-4 ID:SGTC_1283|Compound:0922-0125|FD-Score:3.75|P-value:8.66E-6 ID:SGTC_1347|Compound:1493-0251|FD-Score:2.79|P-value:6.79E-4 ID:SGTC_1366|Compound:1683-0113|FD-Score:-3.15|P-value:1.69E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.30|P-value:8.72E-5 ID:SGTC_1503|Compound:4585-0012|FD-Score:2.79|P-value:6.90E-4 ID:SGTC_1748|Compound:st037455|FD-Score:3.60|P-value:1.83E-5 ID:SGTC_1811|Compound:st050069|FD-Score:2.82|P-value:5.96E-4 ID:SGTC_1885|Compound:st057546|FD-Score:2.78|P-value:7.15E-4 ID:SGTC_1892|Compound:st058411|FD-Score:3.10|P-value:1.88E-4 ID:SGTC_1972|Compound:st070201|FD-Score:3.56|P-value:2.20E-5 ID:SGTC_1980|Compound:st071811|FD-Score:4.74|P-value:2.87E-8 ID:SGTC_1981|Compound:st071826|FD-Score:-4.25|P-value:6.73E-7 ID:SGTC_2106|Compound:5357045|FD-Score:3.79|P-value:6.91E-6 ID:SGTC_2241|Compound:6772625|FD-Score:-3.08|P-value:2.30E-4 ID:SGTC_2355|Compound:9061122|FD-Score:3.05|P-value:2.33E-4 ID:SGTC_2380|Compound:9035439|FD-Score:-3.17|P-value:1.57E-4 ID:SGTC_2393|Compound:9031399|FD-Score:3.80|P-value:6.51E-6 ID:SGTC_2397|Compound:5161689|FD-Score:3.28|P-value:8.38E-5 ID:SGTC_2398|Compound:5228213|FD-Score:2.90|P-value:4.46E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-7.98|P-value:4.81E-20 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:3.64|P-value:1.49E-5 ID:SGTC_2662|Compound:daunorubicin|FD-Score:-3.53|P-value:2.97E-5 ID:SGTC_2670|Compound:beta-carotene|FD-Score:3.26|P-value:9.32E-5 ID:SGTC_2678|Compound:equilin|FD-Score:2.80|P-value:6.57E-4 ID:SGTC_2694|Compound:st077685|FD-Score:-3.27|P-value:1.01E-4 ID:SGTC_2706|Compound:st078239|FD-Score:-3.13|P-value:1.90E-4 ID:SGTC_2864|Compound:9034755|FD-Score:-2.96|P-value:3.87E-4 ID:SGTC_2873|Compound:9040596|FD-Score:3.31|P-value:7.45E-5 ID:SGTC_3348|Compound:9149464|FD-Score:-4.07|P-value:1.86E-6 ID:SGTC_3355|Compound:9151058|FD-Score:-2.90|P-value:4.85E-4 ID:SGTC_42|Compound:0133-0086|FD-Score:-3.01|P-value:3.08E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:-2.85|P-value:5.94E-4 ID:SGTC_167|Compound:3672-0051|FD-Score:-3.01|P-value:3.16E-4 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:3.28|P-value:8.66E-5 ID:SGTC_249|Compound:wiskostatin|FD-Score:3.53|P-value:2.66E-5 ID:SGTC_375|Compound:3970-0961|FD-Score:-4.46|P-value:1.89E-7 ID:SGTC_380|Compound:3970-0968|FD-Score:-3.03|P-value:2.93E-4 ID:SGTC_383|Compound:2324-0395|FD-Score:3.57|P-value:2.13E-5 ID:SGTC_597|Compound:k018-0003|FD-Score:-2.87|P-value:5.49E-4 ID:SGTC_613|Compound:3914-0030|FD-Score:-3.82|P-value:6.94E-6 ID:SGTC_615|Compound:k087-0205|FD-Score:-3.65|P-value:1.69E-5 ID:SGTC_620|Compound:0109-0052|FD-Score:8.32|P-value:9.38E-22 ID:SGTC_625|Compound:k213-0058|FD-Score:5.67|P-value:4.32E-11 ID:SGTC_628|Compound:1636-0253|FD-Score:6.39|P-value:1.27E-13 ID:SGTC_648|Compound:0375-0473|FD-Score:-4.09|P-value:1.62E-6 ID:SGTC_662|Compound:k832-1615|FD-Score:4.32|P-value:3.80E-7 ID:SGTC_737|Compound:1950-0117|FD-Score:2.97|P-value:3.22E-4 ID:SGTC_749|Compound:1000-0129|FD-Score:3.41|P-value:4.67E-5 ID:SGTC_785|Compound:1270-0111|FD-Score:-4.89|P-value:1.27E-8 ID:SGTC_789|Compound:1309-1135|FD-Score:-3.04|P-value:2.72E-4 ID:SGTC_825|Compound:1988-2224|FD-Score:3.08|P-value:2.04E-4 ID:SGTC_981|Compound:1488-1094|FD-Score:-3.04|P-value:2.72E-4 ID:SGTC_986|Compound:1493-0483|FD-Score:3.50|P-value:3.06E-5 ID:SGTC_1214|Compound:0358-0031|FD-Score:2.91|P-value:4.18E-4 ID:SGTC_1239|Compound:0337-0584|FD-Score:2.71|P-value:9.18E-4 ID:SGTC_1283|Compound:0922-0125|FD-Score:3.75|P-value:8.66E-6 ID:SGTC_1347|Compound:1493-0251|FD-Score:2.79|P-value:6.79E-4 ID:SGTC_1366|Compound:1683-0113|FD-Score:-3.15|P-value:1.69E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.30|P-value:8.72E-5 ID:SGTC_1503|Compound:4585-0012|FD-Score:2.79|P-value:6.90E-4 ID:SGTC_1748|Compound:st037455|FD-Score:3.60|P-value:1.83E-5 ID:SGTC_1811|Compound:st050069|FD-Score:2.82|P-value:5.96E-4 ID:SGTC_1885|Compound:st057546|FD-Score:2.78|P-value:7.15E-4 ID:SGTC_1892|Compound:st058411|FD-Score:3.10|P-value:1.88E-4 ID:SGTC_1972|Compound:st070201|FD-Score:3.56|P-value:2.20E-5 ID:SGTC_1980|Compound:st071811|FD-Score:4.74|P-value:2.87E-8 ID:SGTC_1981|Compound:st071826|FD-Score:-4.25|P-value:6.73E-7 ID:SGTC_2106|Compound:5357045|FD-Score:3.79|P-value:6.91E-6 ID:SGTC_2241|Compound:6772625|FD-Score:-3.08|P-value:2.30E-4 ID:SGTC_2355|Compound:9061122|FD-Score:3.05|P-value:2.33E-4 ID:SGTC_2380|Compound:9035439|FD-Score:-3.17|P-value:1.57E-4 ID:SGTC_2393|Compound:9031399|FD-Score:3.80|P-value:6.51E-6 ID:SGTC_2397|Compound:5161689|FD-Score:3.28|P-value:8.38E-5 ID:SGTC_2398|Compound:5228213|FD-Score:2.90|P-value:4.46E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:-7.98|P-value:4.81E-20 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:3.64|P-value:1.49E-5 ID:SGTC_2662|Compound:daunorubicin|FD-Score:-3.53|P-value:2.97E-5 ID:SGTC_2670|Compound:beta-carotene|FD-Score:3.26|P-value:9.32E-5 ID:SGTC_2678|Compound:equilin|FD-Score:2.80|P-value:6.57E-4 ID:SGTC_2694|Compound:st077685|FD-Score:-3.27|P-value:1.01E-4 ID:SGTC_2706|Compound:st078239|FD-Score:-3.13|P-value:1.90E-4 ID:SGTC_2864|Compound:9034755|FD-Score:-2.96|P-value:3.87E-4 ID:SGTC_2873|Compound:9040596|FD-Score:3.31|P-value:7.45E-5 ID:SGTC_3348|Compound:9149464|FD-Score:-4.07|P-value:1.86E-6 ID:SGTC_3355|Compound:9151058|FD-Score:-2.90|P-value:4.85E-4

Top fitness defect scores for YDR119W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_620 0109-0052 8.32 432.0 µM 9.38E-22 1 4.47E-17
2 SGTC_628 1636-0253 6.39 16.0 µM 1.27E-13 sphingolipid biosynthesis & PDR1 7 8.03E-11
3 SGTC_625 k213-0058 5.67 121.0 µM 4.32E-11 translation 40 7.13E-9
4 SGTC_1980 st071811 4.74 45.7 µM 2.87E-8 RPP1 & pyrimidine depletion 8 1.07E-6
5 SGTC_662 k832-1615 4.32 104.0 µM 3.80E-7 12 7.88E-6
6 SGTC_2393 9031399 3.80 185.9 µM 6.51E-6 RNA processing & uracil transport 31 7.09E-5
7 SGTC_2106 5357045 3.79 167.3 µM 6.91E-6 21 7.43E-5
8 SGTC_1283 0922-0125 3.75 16.4 µM 8.66E-6 29 8.84E-5
9 SGTC_2499 avocadenofuran 3.64 51.5 µM 1.49E-5 50 1.35E-4
10 SGTC_1748 st037455 3.60 53.6 µM 1.83E-5 42 1.58E-4
11 SGTC_383 2324-0395 3.57 7.4 µM 2.13E-5 azole & statin 56 1.77E-4
12 SGTC_1972 st070201 3.56 17.4 µM 2.20E-5 35 1.82E-4
13 SGTC_249 wiskostatin 3.53 11.2 µM 2.66E-5 23 2.11E-4
14 SGTC_986 1493-0483 3.50 84.0 µM 3.06E-5 24 2.36E-4
15 SGTC_749 1000-0129 3.41 128.0 µM 4.67E-5 amide catabolism 37 3.27E-4
16 SGTC_2873 9040596 3.31 71.4 µM 7.45E-5 76 4.70E-4
17 SGTC_2397 5161689 3.28 200.0 µM 8.38E-5 65 5.14E-4
18 SGTC_243 5hr heat shock (37°C) + splitomicin 3.28 1.0 µM 8.66E-5 heat shock/prefoldin 83 5.28E-4
19 SGTC_2670 beta-carotene 3.26 100.0 µM 9.32E-5 7 5.59E-4
20 SGTC_1892 st058411 3.10 7.4 µM 1.88E-4 62 9.63E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.160 1.09E-20 YJL050W MTR4 het ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing
0.149 4.50E-18 YBR165W UBS1 hom Ubiquitin-conjugating enzyme suppressor that functions as a general positive regulator of Cdc34p activity; nuclear protein that may represent a link between nucleocytoplasmic transport and ubiquitin ligase activity
0.145 3.27E-17 YJR084W YJR084W hom Protein that forms a complex with Thp3p; may have a role in transcription elongation and/or mRNA splicing; identified as a COP9 signalosome component but phenotype and interactions suggest it may not be involved with the signalosome
0.128 1.24E-13 YER060W-A FCY22 hom Putative purine-cytosine permease, very similar to Fcy2p but cannot substitute for its function
0.120 2.74E-12 YOL071W EMI5 hom Subunit of succinate dehydrogenase, which couples succinate oxidation to ubiquinone reduction; required for FAD cofactor attachment to Sdh1p; mutations in human ortholog PGL2 are associated with neuroendocrine tumors (paraganglioma)
0.120 3.03E-12 YGR123C PPT1 hom Protein serine/threonine phosphatase, regulates Hsp90 chaperone by affecting its ATPase and cochaperone binding activities; has similarity to human phosphatase PP5; present in both the nucleus and cytoplasm; expressed during logarithmic growth
0.116 1.87E-11 YEL010W_d YEL010W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.110 2.13E-10 YBR186W PCH2 hom Nucleolar component of the pachytene checkpoint, which prevents chromosome segregation when recombination and chromosome synapsis are defective; also represses meiotic interhomolog recombination in the rDNA; required for meiotic double-stranded break formation
0.109 2.25E-10 YGL081W_p YGL081W_p hom Putative protein of unknown function; non-essential gene; interacts genetically with CHS5, a gene involved in chitin biosynthesis
0.105 1.30E-9 YBR191W RPL21A hom Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication
0.102 3.49E-9 YIR016W_p YIR016W_p hom Putative protein of unknown function; expression directly regulated by the metabolic and meiotic transcriptional regulator Ume6p; overexpression causes a cell cycle delay or arrest; non-essential gene; YIR016W has a paralog, YOL036W, that arose from the whole genome duplication
0.098 1.15E-8 YBL017C PEP1 hom Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments
0.096 2.68E-8 YML119W_p YML119W_p hom Putative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate
0.096 2.90E-8 YCR102W-A_d YCR102W-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.095 3.30E-8 YER053C PIC2 hom Mitochondrial phosphate carrier, imports inorganic phosphate into mitochondria; functionally redundant with Mir1p but less abundant than Mir1p under normal conditions; expression is induced at high temperature