HIPHOP chemogenomics database

YDR120C / TRM1

tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments

Zygosity: Homozygous strain

fixedexpanded
Profile for YDR120C / TRM1

Click on Significant Values for Screen Details

Top fitness defect scores for YDR120C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_23	0213-0011	23.90	40.0 µM	4.85E-114	copper-dependent oxidative stress	1	1.03E-126
2	SGTC_1941	st076221	17.60	61.8 µM	1.10E-62	sphingolipid biosynthesis & PDR1	1	1.53E-69
3	SGTC_2322	7413319	16.90	38.2 µM	6.16E-58	ERAD & cell cycle	5	2.96E-64
4	SGTC_612	0302-0167	15.60	22.4 µM	7.14E-50	copper-dependent oxidative stress	1	2.80E-55
5	SGTC_1687	st020778	15.00	55.4 µM	2.09E-46		19	2.02E-51
6	SGTC_2752	diclazuril	14.80	33.1 µM	7.05E-45		8	1.01E-49
7	SGTC_2858	9025781	8.15	9.0 µM	5.65E-15	sphingolipid biosynthesis & PDR1	26	1.87E-16
8	SGTC_682	0kpi-0099	7.55	17.0 µM	4.21E-13		14	2.25E-14
9	SGTC_743	1275-0778	7.54	158.0 µM	4.43E-13	excess fatty acid	6	2.38E-14
10	SGTC_318	1488-0629	7.52	67.2 µM	5.12E-13	copper-dependent oxidative stress	8	2.80E-14
11	SGTC_1877	wortmannin	6.78	25.0 µM	6.55E-11		36	6.13E-12
12	SGTC_1875	nocodazole	6.71	6.0 µM	9.78E-11	tubulin folding & SWR complex	23	9.57E-12
13	SGTC_1425	4048-4433	6.12	7.3 µM	3.28E-9	copper-dependent oxidative stress	8	4.74E-10
14	SGTC_2483	5729050	6.02	27.2 µM	5.73E-9		5	8.78E-10
15	SGTC_453	enantio-paf c16	6.02	10.0 µM	5.80E-9		49	8.90E-10
16	SGTC_2651	1327-0036	5.91	5.0 µM	1.07E-8		76	1.76E-9
17	SGTC_528	3492-0019	5.66	172.0 µM	3.96E-8	calcium & mitochondrial duress	133	7.51E-9
18	SGTC_143	4032-1194	5.42	15.7 µM	1.39E-7		23	3.02E-8
19	SGTC_575	k072-0246	5.35	11.9 µM	1.99E-7		9	4.50E-8
20	SGTC_1543	azetidyl-2-carboxylic acid	5.28	198.0 µM	2.81E-7		1	6.59E-8

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.442	1.27E-160	YGR164W_d	YGR164W_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.374	4.33E-112	YGL232W	TAN1	hom	Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress
0.359	1.75E-102	YBL079W	NUP170	hom	Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication
0.342	2.67E-92	YMR031W-A_d	YMR031W-A_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C
0.334	2.40E-88	YLR204W	QRI5	hom	Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA
0.330	4.57E-86	YLR087C	CSF1	hom	Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.284	4.12E-63	YDR392W	SPT3	hom	Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters
0.274	8.76E-59	YNL237W	YTP1	hom	Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins
0.267	6.16E-56	YLR055C	SPT8	hom	Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters
0.259	1.23E-52	YJR074W	MOG1	hom	Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p
0.256	2.72E-51	YOL050C_d	YOL050C_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO)
0.247	6.93E-48	YGL025C	PGD1	hom	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor
0.243	2.84E-46	YIL170W	HXT12	hom	Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family
0.224	1.60E-39	YGL013C	PDR1	hom	Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication
0.223	4.00E-39	YPL265W	DIP5	hom	Dicarboxylic amino acid permease; mediates high-affinity and high-capacity transport of L-glutamate and L-aspartate; also a transporter for Gln, Asn, Ser, Ala, and Gly; relocalizes from plasma membrane to vacuole upon DNA replication stress