tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments
Zygosity: Homozygous strain
fixedexpanded
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Top fitness defect scores for YDR120C deletion by condition
Download Fitness data (tab-delimited text) (excel) |
Correlation | pval | ORF | Gene | Zygosity | Description |
---|---|---|---|---|---|
0.442 | 1.27E-160 | YGR164W_d | YGR164W_d | hom | Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data |
0.374 | 4.33E-112 | YGL232W | TAN1 | hom | Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress |
0.359 | 1.75E-102 | YBL079W | NUP170 | hom | Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication |
0.342 | 2.67E-92 | YMR031W-A_d | YMR031W-A_d | hom | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; null mutant displays shortened telomeres; partially overlaps the uncharacterized ORF YMR031C |
0.334 | 2.40E-88 | YLR204W | QRI5 | hom | Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA |
0.330 | 4.57E-86 | YLR087C | CSF1 | hom | Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies |
0.284 | 4.12E-63 | YDR392W | SPT3 | hom | Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters |
0.274 | 8.76E-59 | YNL237W | YTP1 | hom | Probable type-III integral membrane protein of unknown function, has regions of similarity to mitochondrial electron transport proteins |
0.267 | 6.16E-56 | YLR055C | SPT8 | hom | Subunit of the SAGA transcriptional regulatory complex but not present in SAGA-like complex SLIK/SALSA, required for SAGA-mediated inhibition at some promoters |
0.259 | 1.23E-52 | YJR074W | MOG1 | hom | Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p |
0.256 | 2.72E-51 | YOL050C_d | YOL050C_d | hom | Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified gene GAL11; deletion confers sensitivity to 4-(N-(S-glutathionylacetyl)amino) phenylarsenoxide (GSAO) |
0.247 | 6.93E-48 | YGL025C | PGD1 | hom | Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for basal and activated transcription; direct target of Cyc8p-Tup1p transcriptional corepressor |
0.243 | 2.84E-46 | YIL170W | HXT12 | hom | Possible pseudogene in strain S288C; YIL170W/HXT12 and the adjacent ORF, YIL171W, together encode a non-functional member of the hexose transporter family |
0.224 | 1.60E-39 | YGL013C | PDR1 | hom | Transcription factor that regulates the pleiotropic drug response; zinc cluster protein that is a master regulator involved in recruiting other zinc cluster proteins to pleiotropic drug response elements (PDREs) to fine tune the regulation of multidrug resistance genes; PDR1 has a paralog, PDR3, that arose from the whole genome duplication |
0.223 | 4.00E-39 | YPL265W | DIP5 | hom | Dicarboxylic amino acid permease; mediates high-affinity and high-capacity transport of L-glutamate and L-aspartate; also a transporter for Gln, Asn, Ser, Ala, and Gly; relocalizes from plasma membrane to vacuole upon DNA replication stress |