YDR121W / DPB4

Subunit of DNA pol epsilon and of ISW2 chromatin accessibility complex; involved in both chromosomal DNA replication and inheritance of telomeric silencing; stabilizes the interaction of Pol epsilon with primer-template DNA, positively affecting the processivity of the polymerase and exonuclease activities of Pol epsilon; interacts with extranucleosomal DNA and acts as anchor point for ISW2 complex that retains its position on DNA during nucleosome mobilization

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR121W / DPB4

Click on Significant Values for Screen Details ID:SGTC_514|Compound:4466-0038|FD-Score:13.20|P-value:1.32E-32 ID:SGTC_839|Compound:0122-0028|FD-Score:5.83|P-value:8.23E-8 ID:SGTC_916|Compound:4466-0038|FD-Score:5.20|P-value:1.51E-6 ID:SGTC_962|Compound:1254-0300|FD-Score:5.07|P-value:2.55E-6 ID:SGTC_983|Compound:1491-0821|FD-Score:3.76|P-value:3.50E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:-3.54|P-value:9.02E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:4.66|P-value:1.35E-5 ID:SGTC_1431|Compound:4049-0260|FD-Score:3.45|P-value:9.17E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:4.36|P-value:4.39E-5 ID:SGTC_1521|Compound:st012942|FD-Score:-5.67|P-value:2.60E-7 ID:SGTC_1846|Compound:st056244|FD-Score:-4.52|P-value:3.33E-5 ID:SGTC_2023|Compound:5144124|FD-Score:4.44|P-value:3.27E-5 ID:SGTC_2584|Compound:androsterone|FD-Score:4.19|P-value:7.97E-5 ID:SGTC_3071|Compound:9117417|FD-Score:-4.03|P-value:1.92E-4 ID:SGTC_3199|Compound:9111318|FD-Score:-3.62|P-value:7.23E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:13.20|P-value:1.32E-32 ID:SGTC_839|Compound:0122-0028|FD-Score:5.83|P-value:8.23E-8 ID:SGTC_916|Compound:4466-0038|FD-Score:5.20|P-value:1.51E-6 ID:SGTC_962|Compound:1254-0300|FD-Score:5.07|P-value:2.55E-6 ID:SGTC_983|Compound:1491-0821|FD-Score:3.76|P-value:3.50E-4 ID:SGTC_1119|Compound:parthenolide|FD-Score:-3.54|P-value:9.02E-4 ID:SGTC_1373|Compound:2887-0003|FD-Score:4.66|P-value:1.35E-5 ID:SGTC_1431|Compound:4049-0260|FD-Score:3.45|P-value:9.17E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:4.36|P-value:4.39E-5 ID:SGTC_1521|Compound:st012942|FD-Score:-5.67|P-value:2.60E-7 ID:SGTC_1846|Compound:st056244|FD-Score:-4.52|P-value:3.33E-5 ID:SGTC_2023|Compound:5144124|FD-Score:4.44|P-value:3.27E-5 ID:SGTC_2584|Compound:androsterone|FD-Score:4.19|P-value:7.97E-5 ID:SGTC_3071|Compound:9117417|FD-Score:-4.03|P-value:1.92E-4 ID:SGTC_3199|Compound:9111318|FD-Score:-3.62|P-value:7.23E-4

Top fitness defect scores for YDR121W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_514 4466-0038 13.20 2.6 nM 1.32E-32 cell wall 13 2.93E-40
2 SGTC_839 0122-0028 5.83 230.0 µM 8.23E-8 RSC complex & mRNA processing 7 2.77E-9
3 SGTC_916 4466-0038 5.20 42.0 nM 1.51E-6 cell wall 35 1.02E-7
4 SGTC_962 1254-0300 5.07 21.5 µM 2.55E-6 10 1.96E-7
5 SGTC_1373 2887-0003 4.66 58.5 µM 1.35E-5 Golgi 10 1.54E-6
6 SGTC_2023 5144124 4.44 124.0 µM 3.27E-5 17 4.59E-6
7 SGTC_1468 k284-1086 4.36 134.0 µM 4.39E-5 DNA intercalators 24 6.60E-6
8 SGTC_2584 androsterone 4.19 48.7 µM 7.97E-5 cell wall 118 1.38E-5
9 SGTC_983 1491-0821 3.76 12.2 µM 3.50E-4 17 8.51E-5
10 SGTC_1431 4049-0260 3.45 9.1 µM 9.17E-4 11 2.78E-4
11 SGTC_1915 st067105 3.40 30.6 µM 0.00108 RNA pol III & RNase P/MRP 72 3.39E-4
12 SGTC_2961 9083290 3.39 32.5 µM 0.00110 35 3.47E-4
13 SGTC_2152 5568786 3.31 200.0 µM 0.00142 30 4.74E-4
14 SGTC_3350 9150499 3.28 3.6 µM 0.00151 cell wall 87 5.12E-4
15 SGTC_2109 5483026 3.27 200.0 µM 0.00157 RSC complex & mRNA processing 63 5.37E-4
16 SGTC_906 1165-0455 3.26 526.0 µM 0.00160 26 5.50E-4
17 SGTC_448 monastrol 3.23 171.0 µM 0.00174 19 6.09E-4
18 SGTC_1864 st057645 3.21 30.4 µM 0.00186 amide catabolism 29 6.59E-4
19 SGTC_3196 9111891 3.16 49.5 µM 0.00213 mitochondrial processes 41 7.80E-4
20 SGTC_2756 carbadox 3.12 75.1 µM 0.00242 110 9.14E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.381 4.27E-116 YMR036C MIH1 hom Protein tyrosine phosphatase involved in cell cycle control; regulates the phosphorylation state of Cdc28p; homolog of S. pombe cdc25
0.366 1.36E-106 YBL085W BOI1 hom Protein implicated in polar growth; functionally redundant with Boi2p; interacts with bud-emergence protein Bem1p; contains an SH3 (src homology 3) domain and a PH (pleckstrin homology) domain; relocalizes from bud neck to cytoplasm upon DNA replication stress; BOI1 has a paralog, BOI2, that arose from the whole genome duplication
0.350 6.54E-97 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.335 1.28E-88 YMR121C RPL15B hom Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress
0.309 4.62E-75 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.300 7.68E-71 YBR020W GAL1 hom Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication
0.300 9.63E-71 YMR124W_p YMR124W_p hom Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.300 9.51E-71 YGL033W HOP2 hom Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair
0.289 1.48E-65 YOR091W TMA46 hom Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p
0.266 2.76E-55 YML119W_p YML119W_p hom Putative protein of unknown function; YML119W is not an essential gene; potential Cdc28p substrate
0.262 7.51E-54 YBR134W_d YBR134W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.253 5.50E-50 YLR102C APC9 hom Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
0.252 9.42E-50 YPR061C JID1 hom Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae
0.246 3.29E-47 YGR211W ZPR1 het Essential protein with two zinc fingers; present in the nucleus of growing cells but relocates to the cytoplasm in starved cells via a process mediated by Cpr1p; binds to translation elongation factor eEF-1 (Tef1p); relative distribution to the nucleus increases upon DNA replication stress
0.236 1.42E-43 YEL061C CIN8 hom Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation