YDR125C / ECM18

Protein of unknown function; ECM18 has a paralog, ICT1, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR125C / ECM18

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:-5.07|P-value:5.65E-6 ID:SGTC_91|Compound:0417-1751|FD-Score:4.56|P-value:3.41E-5 ID:SGTC_103|Compound:0844-0104|FD-Score:-4.74|P-value:2.05E-5 ID:SGTC_115|Compound:0527-0192|FD-Score:3.92|P-value:3.11E-4 ID:SGTC_126|Compound:0417-1624|FD-Score:3.70|P-value:6.05E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:4.84|P-value:1.18E-5 ID:SGTC_221|Compound:trifluoperazine|FD-Score:8.21|P-value:4.11E-13 ID:SGTC_445|Compound:e6 berbamine|FD-Score:3.72|P-value:5.76E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-6.63|P-value:4.55E-9 ID:SGTC_467|Compound:ro 31-8220|FD-Score:4.54|P-value:3.74E-5 ID:SGTC_568|Compound:1193-0097|FD-Score:-7.16|P-value:2.72E-10 ID:SGTC_658|Compound:3253-0951|FD-Score:-3.79|P-value:5.25E-4 ID:SGTC_711|Compound:k292-0785|FD-Score:3.92|P-value:3.03E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:4.83|P-value:1.22E-5 ID:SGTC_913|Compound:3487-1245|FD-Score:3.80|P-value:4.47E-4 ID:SGTC_1023|Compound:4482-3888|FD-Score:-4.03|P-value:2.45E-4 ID:SGTC_1142|Compound:3253-1408|FD-Score:6.39|P-value:1.25E-8 ID:SGTC_1145|Compound:3546-0597|FD-Score:4.82|P-value:1.31E-5 ID:SGTC_1156|Compound:4092-0367|FD-Score:-4.26|P-value:1.14E-4 ID:SGTC_1160|Compound:4092-0570|FD-Score:-4.98|P-value:8.17E-6 ID:SGTC_1205|Compound:4161-2785|FD-Score:-3.59|P-value:9.50E-4 ID:SGTC_1212|Compound:0520-0165|FD-Score:3.87|P-value:3.65E-4 ID:SGTC_1338|Compound:1486-1223|FD-Score:3.69|P-value:6.28E-4 ID:SGTC_1355|Compound:1499-0035|FD-Score:4.49|P-value:4.35E-5 ID:SGTC_1356|Compound:1502-0781|FD-Score:3.97|P-value:2.62E-4 ID:SGTC_1361|Compound:1608-0041|FD-Score:3.75|P-value:5.23E-4 ID:SGTC_1491|Compound:4138-0063|FD-Score:-3.86|P-value:4.20E-4 ID:SGTC_1728|Compound:st035614|FD-Score:4.00|P-value:2.40E-4 ID:SGTC_1760|Compound:st045453|FD-Score:4.42|P-value:5.61E-5 ID:SGTC_1766|Compound:st045414|FD-Score:4.73|P-value:1.83E-5 ID:SGTC_1800|Compound:st050079|FD-Score:5.54|P-value:6.57E-7 ID:SGTC_1801|Compound:st050089|FD-Score:6.47|P-value:8.24E-9 ID:SGTC_1803|Compound:st050068|FD-Score:5.40|P-value:1.22E-6 ID:SGTC_1949|Compound:st076024|FD-Score:4.21|P-value:1.16E-4 ID:SGTC_2060|Compound:5238662|FD-Score:5.45|P-value:9.76E-7 ID:SGTC_2062|Compound:5245942|FD-Score:4.12|P-value:1.62E-4 ID:SGTC_2065|Compound:5221596|FD-Score:3.94|P-value:2.93E-4 ID:SGTC_2182|Compound:6325192|FD-Score:-4.43|P-value:6.23E-5 ID:SGTC_2402|Compound:5553605|FD-Score:3.61|P-value:7.95E-4 ID:SGTC_2431|Compound:5490079|FD-Score:-4.16|P-value:1.60E-4 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:-3.75|P-value:5.85E-4 ID:SGTC_2608|Compound:thymoquinone|FD-Score:3.67|P-value:6.68E-4 ID:SGTC_2635|Compound:hydroquinidine|FD-Score:-3.83|P-value:4.56E-4 ID:SGTC_2639|Compound:lactacystin|FD-Score:3.59|P-value:8.59E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:-3.63|P-value:8.39E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:7.31|P-value:9.40E-11 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:-3.91|P-value:3.54E-4 ID:SGTC_2830|Compound:7992007|FD-Score:-5.09|P-value:5.19E-6 ID:SGTC_2952|Compound:9073731|FD-Score:-3.70|P-value:6.92E-4 ID:SGTC_3037|Compound:9091026|FD-Score:4.13|P-value:1.52E-4 ID:SGTC_3152|Compound:9099253|FD-Score:3.57|P-value:8.99E-4 ID:SGTC_3157|Compound:9099274|FD-Score:4.10|P-value:1.71E-4 ID:SGTC_3245|Compound:9134821|FD-Score:3.91|P-value:3.20E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-5.07|P-value:5.65E-6 ID:SGTC_91|Compound:0417-1751|FD-Score:4.56|P-value:3.41E-5 ID:SGTC_103|Compound:0844-0104|FD-Score:-4.74|P-value:2.05E-5 ID:SGTC_115|Compound:0527-0192|FD-Score:3.92|P-value:3.11E-4 ID:SGTC_126|Compound:0417-1624|FD-Score:3.70|P-value:6.05E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:4.84|P-value:1.18E-5 ID:SGTC_221|Compound:trifluoperazine|FD-Score:8.21|P-value:4.11E-13 ID:SGTC_445|Compound:e6 berbamine|FD-Score:3.72|P-value:5.76E-4 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-6.63|P-value:4.55E-9 ID:SGTC_467|Compound:ro 31-8220|FD-Score:4.54|P-value:3.74E-5 ID:SGTC_568|Compound:1193-0097|FD-Score:-7.16|P-value:2.72E-10 ID:SGTC_658|Compound:3253-0951|FD-Score:-3.79|P-value:5.25E-4 ID:SGTC_711|Compound:k292-0785|FD-Score:3.92|P-value:3.03E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:4.83|P-value:1.22E-5 ID:SGTC_913|Compound:3487-1245|FD-Score:3.80|P-value:4.47E-4 ID:SGTC_1023|Compound:4482-3888|FD-Score:-4.03|P-value:2.45E-4 ID:SGTC_1142|Compound:3253-1408|FD-Score:6.39|P-value:1.25E-8 ID:SGTC_1145|Compound:3546-0597|FD-Score:4.82|P-value:1.31E-5 ID:SGTC_1156|Compound:4092-0367|FD-Score:-4.26|P-value:1.14E-4 ID:SGTC_1160|Compound:4092-0570|FD-Score:-4.98|P-value:8.17E-6 ID:SGTC_1205|Compound:4161-2785|FD-Score:-3.59|P-value:9.50E-4 ID:SGTC_1212|Compound:0520-0165|FD-Score:3.87|P-value:3.65E-4 ID:SGTC_1338|Compound:1486-1223|FD-Score:3.69|P-value:6.28E-4 ID:SGTC_1355|Compound:1499-0035|FD-Score:4.49|P-value:4.35E-5 ID:SGTC_1356|Compound:1502-0781|FD-Score:3.97|P-value:2.62E-4 ID:SGTC_1361|Compound:1608-0041|FD-Score:3.75|P-value:5.23E-4 ID:SGTC_1491|Compound:4138-0063|FD-Score:-3.86|P-value:4.20E-4 ID:SGTC_1728|Compound:st035614|FD-Score:4.00|P-value:2.40E-4 ID:SGTC_1760|Compound:st045453|FD-Score:4.42|P-value:5.61E-5 ID:SGTC_1766|Compound:st045414|FD-Score:4.73|P-value:1.83E-5 ID:SGTC_1800|Compound:st050079|FD-Score:5.54|P-value:6.57E-7 ID:SGTC_1801|Compound:st050089|FD-Score:6.47|P-value:8.24E-9 ID:SGTC_1803|Compound:st050068|FD-Score:5.40|P-value:1.22E-6 ID:SGTC_1949|Compound:st076024|FD-Score:4.21|P-value:1.16E-4 ID:SGTC_2060|Compound:5238662|FD-Score:5.45|P-value:9.76E-7 ID:SGTC_2062|Compound:5245942|FD-Score:4.12|P-value:1.62E-4 ID:SGTC_2065|Compound:5221596|FD-Score:3.94|P-value:2.93E-4 ID:SGTC_2182|Compound:6325192|FD-Score:-4.43|P-value:6.23E-5 ID:SGTC_2402|Compound:5553605|FD-Score:3.61|P-value:7.95E-4 ID:SGTC_2431|Compound:5490079|FD-Score:-4.16|P-value:1.60E-4 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:-3.75|P-value:5.85E-4 ID:SGTC_2608|Compound:thymoquinone|FD-Score:3.67|P-value:6.68E-4 ID:SGTC_2635|Compound:hydroquinidine|FD-Score:-3.83|P-value:4.56E-4 ID:SGTC_2639|Compound:lactacystin|FD-Score:3.59|P-value:8.59E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:-3.63|P-value:8.39E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:7.31|P-value:9.40E-11 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:-3.91|P-value:3.54E-4 ID:SGTC_2830|Compound:7992007|FD-Score:-5.09|P-value:5.19E-6 ID:SGTC_2952|Compound:9073731|FD-Score:-3.70|P-value:6.92E-4 ID:SGTC_3037|Compound:9091026|FD-Score:4.13|P-value:1.52E-4 ID:SGTC_3152|Compound:9099253|FD-Score:3.57|P-value:8.99E-4 ID:SGTC_3157|Compound:9099274|FD-Score:4.10|P-value:1.71E-4 ID:SGTC_3245|Compound:9134821|FD-Score:3.91|P-value:3.20E-4

Top fitness defect scores for YDR125C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_221 trifluoperazine 8.21 10.3 µM 4.11E-13 NEO1-PIK1 82 1.08E-16
2 SGTC_2688 kanamycin b 7.31 96.7 µM 9.40E-11 calcium & mitochondrial duress 93 1.36E-13
3 SGTC_1801 st050089 6.47 28.4 µM 8.24E-9 NEO1-PIK1 43 4.78E-11
4 SGTC_1142 3253-1408 6.39 9.8 µM 1.25E-8 1 8.25E-11
5 SGTC_1800 st050079 5.54 29.1 µM 6.57E-7 NEO1-PIK1 54 1.47E-8
6 SGTC_2060 5238662 5.45 140.0 µM 9.76E-7 11 2.47E-8
7 SGTC_1803 st050068 5.40 52.4 µM 1.22E-6 NEO1-PIK1 102 3.30E-8
8 SGTC_146 3448-8650 4.84 53.1 µM 1.18E-5 NEO1-PIK1 90 6.37E-7
9 SGTC_785 1270-0111 4.83 65.3 µM 1.22E-5 11 6.70E-7
10 SGTC_1145 3546-0597 4.82 10.2 µM 1.31E-5 mitochondrial processes 9 7.36E-7
11 SGTC_1766 st045414 4.73 44.8 µM 1.83E-5 RPP1 & pyrimidine depletion 13 1.14E-6
12 SGTC_91 0417-1751 4.56 58.4 µM 3.41E-5 Golgi 35 2.55E-6
13 SGTC_467 ro 31-8220 4.54 72.8 µM 3.74E-5 calcium & mitochondrial duress 56 2.88E-6
14 SGTC_1355 1499-0035 4.49 1.7 µM 4.35E-5 8 3.50E-6
15 SGTC_1760 st045453 4.42 10.4 µM 5.61E-5 NEO1-PIK1 126 4.87E-6
16 SGTC_1949 st076024 4.21 69.1 µM 1.16E-4 16 1.25E-5
17 SGTC_3037 9091026 4.13 49.5 µM 1.52E-4 NEO1-PIK1 113 1.78E-5
18 SGTC_2062 5245942 4.12 200.0 µM 1.62E-4 14 1.93E-5
19 SGTC_3157 9099274 4.10 49.5 µM 1.71E-4 17 2.07E-5
20 SGTC_1728 st035614 4.00 33.8 µM 2.40E-4 45 3.21E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.261 1.45E-53 YOR357C SNX3 hom Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p
0.257 1.03E-51 YPL202C AFT2 hom Iron-regulated transcriptional activator; activates genes involved in intracellular iron use and required for iron homeostasis and resistance to oxidative stress; AFT2 has a paralog, AFT1, that arose from the whole genome duplication
0.254 2.17E-50 YPR091C NVJ2 hom Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments
0.247 8.49E-48 YJL052W TDH1 hom Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress
0.247 1.06E-47 YPL145C KES1 hom One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication
0.246 1.91E-47 YNL183C NPR1 hom Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex
0.241 1.70E-45 YAR042W SWH1 hom Protein similar to mammalian oxysterol-binding protein; contains ankyrin repeats; localizes to the Golgi and the nucleus-vacuole junction
0.229 5.23E-41 YJL154C VPS35 hom Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.220 5.79E-38 YJL053W PEP8 hom Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
0.218 2.22E-37 YER120W SCS2 hom Integral ER membrane protein, regulates phospholipid metabolism; one of 6 proteins (Ist2p, Scs2p, Scs22p, Tcb1p, Tcb2p, Tcb3p) that connect ER to the plasma membrane (PM) and regulate PI4P levels by controlling access of Sac1p phosphatase to its substrate PI4P in the PM; interacts with FFAT motif of Opi1p; involved in telomeric silencing; null shows inositol auxotrophy above 34 deg C; VAP homolog; SCS2 has a paralog, SCS22, that arose from the whole genome duplication
0.217 6.59E-37 YHR012W VPS29 hom Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
0.215 1.93E-36 YNL267W PIK1 het Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
0.214 7.01E-36 YDL033C SLM3 hom tRNA-specific 2-thiouridylase, responsible for 2-thiolation of the wobble base of mitochondrial tRNAs; human ortholog is implicated in myoclonus epilepsy associated with ragged red fibers (MERRF)
0.211 4.15E-35 YHR108W GGA2 hom Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin
0.202 2.81E-32 YER119C-A_d YER119C-A_d hom Dubious open reading frame, not conserved in closely related Saccharomyces species; deletion mutation blocks replication of Brome mosaic virus in S. cerevisiae, but this is likely due to effects on the overlapping gene SCS2