YDR135C / YCF1

Vacuolar glutathione S-conjugate transporter; member of the ATP-binding cassette family; has a role in detoxifying metals such as cadmium, mercury, and arsenite; also transports unconjugated bilirubin, selenodigluthatione, and oxidized glutathione; similar to human cystic fibrosis protein CFTR

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR135C / YCF1

Click on Significant Values for Screen Details ID:SGTC_167|Compound:3672-0051|FD-Score:-3.72|P-value:4.00E-5 ID:SGTC_188|Compound:3229-0966|FD-Score:2.94|P-value:7.58E-4 ID:SGTC_213|Compound:1246-0773|FD-Score:3.56|P-value:6.33E-5 ID:SGTC_391|Compound:gliotoxin|FD-Score:3.24|P-value:2.38E-4 ID:SGTC_493|Compound:zm226600|FD-Score:2.97|P-value:6.64E-4 ID:SGTC_504|Compound:lovastatin|FD-Score:3.42|P-value:1.15E-4 ID:SGTC_534|Compound:k292-0788|FD-Score:3.67|P-value:3.85E-5 ID:SGTC_673|Compound:1082-0474|FD-Score:2.90|P-value:8.73E-4 ID:SGTC_701|Compound:4424-0219|FD-Score:2.92|P-value:7.95E-4 ID:SGTC_723|Compound:4182-0001|FD-Score:3.32|P-value:1.73E-4 ID:SGTC_724|Compound:3910-0327|FD-Score:3.59|P-value:5.40E-5 ID:SGTC_725|Compound:1497-0963|FD-Score:3.65|P-value:4.20E-5 ID:SGTC_728|Compound:3030-5151|FD-Score:-2.97|P-value:8.48E-4 ID:SGTC_730|Compound:1436-0052|FD-Score:-3.52|P-value:9.56E-5 ID:SGTC_774|Compound:k060-0086|FD-Score:2.96|P-value:7.10E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:7.71|P-value:6.73E-17 ID:SGTC_788|Compound:1182-0090|FD-Score:3.32|P-value:1.72E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:2.89|P-value:9.08E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:3.78|P-value:2.30E-5 ID:SGTC_942|Compound:3253-1115|FD-Score:3.35|P-value:1.51E-4 ID:SGTC_979|Compound:1426-2828|FD-Score:4.65|P-value:2.91E-7 ID:SGTC_1206|Compound:3978-0017|FD-Score:3.02|P-value:5.49E-4 ID:SGTC_1207|Compound:2099-0101|FD-Score:3.09|P-value:4.32E-4 ID:SGTC_1355|Compound:1499-0035|FD-Score:3.76|P-value:2.60E-5 ID:SGTC_1465|Compound:k213-0056|FD-Score:3.11|P-value:3.94E-4 ID:SGTC_1506|Compound:4226-2149|FD-Score:2.97|P-value:6.80E-4 ID:SGTC_1518|Compound:st038567|FD-Score:3.66|P-value:3.93E-5 ID:SGTC_1702|Compound:st027038|FD-Score:3.37|P-value:1.41E-4 ID:SGTC_1773|Compound:st048542|FD-Score:3.98|P-value:8.98E-6 ID:SGTC_1830|Compound:st055982|FD-Score:4.77|P-value:1.45E-7 ID:SGTC_1940|Compound:st075990|FD-Score:3.72|P-value:3.00E-5 ID:SGTC_1954|Compound:st077032|FD-Score:-3.49|P-value:1.11E-4 ID:SGTC_2002|Compound:4023503|FD-Score:3.20|P-value:2.75E-4 ID:SGTC_2020|Compound:4017018|FD-Score:-4.62|P-value:4.67E-7 ID:SGTC_2021|Compound:4019055|FD-Score:5.53|P-value:1.38E-9 ID:SGTC_2064|Compound:5220758|FD-Score:-3.16|P-value:4.22E-4 ID:SGTC_2133|Compound:5320837|FD-Score:3.14|P-value:3.52E-4 ID:SGTC_2241|Compound:6772625|FD-Score:-3.05|P-value:6.33E-4 ID:SGTC_2254|Compound:7938975|FD-Score:3.00|P-value:6.11E-4 ID:SGTC_2276|Compound:7947513|FD-Score:-3.49|P-value:1.09E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-3.11|P-value:5.06E-4 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:3.30|P-value:1.83E-4 ID:SGTC_2638|Compound:epoxomicin|FD-Score:-2.95|P-value:9.16E-4 ID:SGTC_2639|Compound:lactacystin|FD-Score:-2.95|P-value:9.16E-4 ID:SGTC_2743|Compound:auranofin|FD-Score:7.49|P-value:4.82E-16 ID:SGTC_2811|Compound:7967822|FD-Score:3.30|P-value:1.84E-4 ID:SGTC_2930|Compound:9013230|FD-Score:3.81|P-value:1.99E-5 ID:SGTC_2940|Compound:9060638|FD-Score:5.26|P-value:7.67E-9 ID:SGTC_2959|Compound:9079155|FD-Score:-2.99|P-value:7.81E-4 ID:SGTC_3094|Compound:9117742|FD-Score:-4.18|P-value:4.69E-6 ID:SGTC_3134|Compound:9127454|FD-Score:-3.38|P-value:1.71E-4 ID:SGTC_3146|Compound:9095609|FD-Score:3.54|P-value:6.82E-5 ID:SGTC_3210|Compound:9128157|FD-Score:-3.85|P-value:2.22E-5 ID:SGTC_3253|Compound:9135987|FD-Score:3.05|P-value:4.90E-4 ID:SGTC_3300|Compound:9124615|FD-Score:3.69|P-value:3.52E-5 ID:SGTC_167|Compound:3672-0051|FD-Score:-3.72|P-value:4.00E-5 ID:SGTC_188|Compound:3229-0966|FD-Score:2.94|P-value:7.58E-4 ID:SGTC_213|Compound:1246-0773|FD-Score:3.56|P-value:6.33E-5 ID:SGTC_391|Compound:gliotoxin|FD-Score:3.24|P-value:2.38E-4 ID:SGTC_493|Compound:zm226600|FD-Score:2.97|P-value:6.64E-4 ID:SGTC_504|Compound:lovastatin|FD-Score:3.42|P-value:1.15E-4 ID:SGTC_534|Compound:k292-0788|FD-Score:3.67|P-value:3.85E-5 ID:SGTC_673|Compound:1082-0474|FD-Score:2.90|P-value:8.73E-4 ID:SGTC_701|Compound:4424-0219|FD-Score:2.92|P-value:7.95E-4 ID:SGTC_723|Compound:4182-0001|FD-Score:3.32|P-value:1.73E-4 ID:SGTC_724|Compound:3910-0327|FD-Score:3.59|P-value:5.40E-5 ID:SGTC_725|Compound:1497-0963|FD-Score:3.65|P-value:4.20E-5 ID:SGTC_728|Compound:3030-5151|FD-Score:-2.97|P-value:8.48E-4 ID:SGTC_730|Compound:1436-0052|FD-Score:-3.52|P-value:9.56E-5 ID:SGTC_774|Compound:k060-0086|FD-Score:2.96|P-value:7.10E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:7.71|P-value:6.73E-17 ID:SGTC_788|Compound:1182-0090|FD-Score:3.32|P-value:1.72E-4 ID:SGTC_805|Compound:3852-0218|FD-Score:2.89|P-value:9.08E-4 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:3.78|P-value:2.30E-5 ID:SGTC_942|Compound:3253-1115|FD-Score:3.35|P-value:1.51E-4 ID:SGTC_979|Compound:1426-2828|FD-Score:4.65|P-value:2.91E-7 ID:SGTC_1206|Compound:3978-0017|FD-Score:3.02|P-value:5.49E-4 ID:SGTC_1207|Compound:2099-0101|FD-Score:3.09|P-value:4.32E-4 ID:SGTC_1355|Compound:1499-0035|FD-Score:3.76|P-value:2.60E-5 ID:SGTC_1465|Compound:k213-0056|FD-Score:3.11|P-value:3.94E-4 ID:SGTC_1506|Compound:4226-2149|FD-Score:2.97|P-value:6.80E-4 ID:SGTC_1518|Compound:st038567|FD-Score:3.66|P-value:3.93E-5 ID:SGTC_1702|Compound:st027038|FD-Score:3.37|P-value:1.41E-4 ID:SGTC_1773|Compound:st048542|FD-Score:3.98|P-value:8.98E-6 ID:SGTC_1830|Compound:st055982|FD-Score:4.77|P-value:1.45E-7 ID:SGTC_1940|Compound:st075990|FD-Score:3.72|P-value:3.00E-5 ID:SGTC_1954|Compound:st077032|FD-Score:-3.49|P-value:1.11E-4 ID:SGTC_2002|Compound:4023503|FD-Score:3.20|P-value:2.75E-4 ID:SGTC_2020|Compound:4017018|FD-Score:-4.62|P-value:4.67E-7 ID:SGTC_2021|Compound:4019055|FD-Score:5.53|P-value:1.38E-9 ID:SGTC_2064|Compound:5220758|FD-Score:-3.16|P-value:4.22E-4 ID:SGTC_2133|Compound:5320837|FD-Score:3.14|P-value:3.52E-4 ID:SGTC_2241|Compound:6772625|FD-Score:-3.05|P-value:6.33E-4 ID:SGTC_2254|Compound:7938975|FD-Score:3.00|P-value:6.11E-4 ID:SGTC_2276|Compound:7947513|FD-Score:-3.49|P-value:1.09E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:-3.11|P-value:5.06E-4 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:3.30|P-value:1.83E-4 ID:SGTC_2638|Compound:epoxomicin|FD-Score:-2.95|P-value:9.16E-4 ID:SGTC_2639|Compound:lactacystin|FD-Score:-2.95|P-value:9.16E-4 ID:SGTC_2743|Compound:auranofin|FD-Score:7.49|P-value:4.82E-16 ID:SGTC_2811|Compound:7967822|FD-Score:3.30|P-value:1.84E-4 ID:SGTC_2930|Compound:9013230|FD-Score:3.81|P-value:1.99E-5 ID:SGTC_2940|Compound:9060638|FD-Score:5.26|P-value:7.67E-9 ID:SGTC_2959|Compound:9079155|FD-Score:-2.99|P-value:7.81E-4 ID:SGTC_3094|Compound:9117742|FD-Score:-4.18|P-value:4.69E-6 ID:SGTC_3134|Compound:9127454|FD-Score:-3.38|P-value:1.71E-4 ID:SGTC_3146|Compound:9095609|FD-Score:3.54|P-value:6.82E-5 ID:SGTC_3210|Compound:9128157|FD-Score:-3.85|P-value:2.22E-5 ID:SGTC_3253|Compound:9135987|FD-Score:3.05|P-value:4.90E-4 ID:SGTC_3300|Compound:9124615|FD-Score:3.69|P-value:3.52E-5

Top fitness defect scores for YDR135C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_778 3456-2434 7.71 196.0 µM 6.73E-17 mitochondrial response to ROS 10 6.34E-15
2 SGTC_2743 auranofin 7.49 4.3 µM 4.82E-16 RPP1 & pyrimidine depletion 5 3.54E-14
3 SGTC_2021 4019055 5.53 173.0 µM 1.38E-9 endomembrane recycling 53 1.57E-8
4 SGTC_2940 9060638 5.26 45.9 µM 7.67E-9 amide catabolism 4 7.02E-8
5 SGTC_1830 st055982 4.77 50.3 µM 1.45E-7 6 9.18E-7
6 SGTC_979 1426-2828 4.65 121.0 µM 2.91E-7 12 1.69E-6
7 SGTC_1773 st048542 3.98 73.6 µM 8.98E-6 41 3.40E-5
8 SGTC_2930 9013230 3.81 14.9 µM 1.99E-5 heme biosynthesis & mitochondrial translocase 45 6.82E-5
9 SGTC_915 methyl methanesulfonate (MMS) 3.78 110.0 mM 2.30E-5 DNA damage response 51 7.73E-5
10 SGTC_1355 1499-0035 3.76 1.7 µM 2.60E-5 21 8.61E-5
11 SGTC_1940 st075990 3.72 51.1 µM 3.00E-5 60S ribosome export 28 9.78E-5
12 SGTC_3300 9124615 3.69 50.5 µM 3.52E-5 23 1.12E-4
13 SGTC_534 k292-0788 3.67 63.4 µM 3.85E-5 mitochondrial processes 37 1.21E-4
14 SGTC_1518 st038567 3.66 90.8 µM 3.93E-5 17 1.24E-4
15 SGTC_725 1497-0963 3.65 19.0 µM 4.20E-5 copper-dependent oxidative stress 25 1.31E-4
16 SGTC_724 3910-0327 3.59 137.0 µM 5.40E-5 ERG2 28 1.63E-4
17 SGTC_213 1246-0773 3.56 967.0 nM 6.33E-5 32 1.88E-4
18 SGTC_3146 9095609 3.54 49.5 µM 6.82E-5 28 2.00E-4
19 SGTC_504 lovastatin 3.42 124.0 µM 1.15E-4 25 3.16E-4
20 SGTC_1702 st027038 3.37 51.0 µM 1.41E-4 29 3.79E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.132 1.97E-14 YLR408C_p BLS1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome; YLR408C is not an essential gene
0.114 3.25E-11 YGR181W TIM13 hom Mitochondrial intermembrane space protein, forms a complex with Tim8p that delivers a subset of hydrophobic proteins to the TIM22 complex for insertion into the inner membrane
0.108 2.91E-10 YDL138W RGT2 hom Plasma membrane high glucose sensor that regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; RGT2 has a paralog, SNF3, that arose from the whole genome duplication
0.104 1.81E-9 YDR310C SUM1 hom Transcriptional repressor required for mitotic repression of middle sporulation-specific genes; also acts as general replication initiation factor; involved in telomere maintenance, chromatin silencing; regulated by pachytene checkpoint
0.096 2.67E-8 YOR079C ATX2 hom Golgi membrane protein involved in manganese homeostasis; overproduction suppresses the sod1 (copper, zinc superoxide dismutase) null mutation
0.095 3.66E-8 YGL203C KEX1 hom Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins
0.095 3.98E-8 YPL047W SGF11 hom Integral subunit of SAGA histone acetyltransferase complex, regulates transcription of a subset of SAGA-regulated genes, required for the Ubp8p association with SAGA and for H2B deubiquitylation
0.093 7.26E-8 YHR008C SOD2 hom Mitochondrial manganese superoxide dismutase, protects cells against oxygen toxicity; phosphorylated
0.093 7.47E-8 YLR277C YSH1 het Putative endoribonuclease; subunit of the mRNA cleavage and polyadenylation specificity complex; required for 3' processing, splicing, and transcriptional termination of mRNAs and snoRNAs; protein abundance increases in response to DNA replication stress; YSH1 has a paralog, SYC1, that arose from the whole genome duplication
0.092 9.50E-8 YIL065C FIS1 hom Protein involved in mitochondrial membrane fission and peroxisome abundance; required for localization of Dnm1p and Mdv1p during mitochondrial division; mediates ethanol-induced apoptosis and ethanol-induced mitochondrial fragmentation
0.091 1.49E-7 YDR357C_p CNL1_p hom Protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.088 3.32E-7 YDR084C TVP23 hom Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
0.086 5.83E-7 YDR467C_d YDR467C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.086 5.87E-7 YPL110C GDE1 hom Glycerophosphocholine (GroPCho) phosphodiesterase; hydrolyzes GroPCho to choline and glycerolphosphate, for use as a phosphate source and as a precursor for phosphocholine synthesis; may interact with ribosomes
0.086 6.37E-7 YGL079W_p KXD1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome