HIPHOP chemogenomics database

YDR136C_d / VPS61

Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 4% of ORF overlaps the verified gene RGP1; deletion causes a vacuolar protein sorting defect

Zygosity: Homozygous strain

fixedexpanded
Profile for YDR136C / VPS61

Click on Significant Values for Screen Details

Top fitness defect scores for YDR136C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_1319	1306-0155	12.50	43.7 µM	3.99E-15		1	2.24E-36
2	SGTC_2397	5161689	9.52	200.0 µM	1.82E-9		2	8.46E-22
3	SGTC_528	3492-0019	8.83	172.0 µM	2.23E-8	calcium & mitochondrial duress	57	5.15E-19
4	SGTC_2440	5628896	8.51	157.8 µM	6.71E-8		2	8.68E-18
5	SGTC_748	0136-0228	8.01	77.9 µM	3.52E-7	redox potentiating	20	5.97E-16
6	SGTC_1306	1185-0270	7.32	9.0 µM	2.86E-6	Golgi	22	1.23E-13
7	SGTC_1313	1220-0047	7.28	237.0 µM	3.22E-6		9	1.67E-13
8	SGTC_133	0724-0033	7.07	378.9 µM	5.83E-6		4	7.52E-13
9	SGTC_2443	5493264	6.54	126.7 µM	2.49E-5		5	2.98E-11
10	SGTC_1349	flufenamic acid	6.31	69.0 µM	4.55E-5		3	1.36E-10
11	SGTC_132	0417-1715	6.27	25.8 µM	5.07E-5	RPP1 & pyrimidine depletion	4	1.79E-10
12	SGTC_1510	1123-0200	6.00	71.5 µM	9.96E-5		16	9.77E-10
13	SGTC_1732	st034308	5.99	51.8 µM	1.02E-4		2	1.03E-9
14	SGTC_31	0508-1974	5.68	57.2 µM	2.16E-4		2	6.79E-9
15	SGTC_1927	st060202	5.67	13.8 µM	2.22E-4		2	7.31E-9
16	SGTC_1717	st033143	5.51	58.0 µM	3.16E-4		17	1.77E-8
17	SGTC_1038	k292-0204	5.49	154.0 µM	3.37E-4		4	2.07E-8
18	SGTC_2900	5328528	5.42	85.3 µM	3.94E-4	Golgi	10	3.05E-8
19	SGTC_1274	0865-0114	5.30	173.0 µM	5.12E-4		5	5.88E-8
20	SGTC_2437	5741531	5.30	144.2 µM	5.12E-4		16	5.90E-8

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.545	1.18E-257	YDR137W	RGP1	hom	Subunit of a Golgi membrane exchange factor (Ric1p-Rgp1p) that catalyzes nucleotide exchange on Ypt6p
0.497	8.94E-208	YLR262C	YPT6	hom	Rab family GTPase, Ras-like GTP binding protein involved in the secretory pathway, required for fusion of endosome-derived vesicles with the late Golgi, maturation of the vacuolar carboxypeptidase Y; has similarity to the human GTPase, Rab6
0.488	3.28E-200	YLR261C_d	VPS63_d	hom	Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene YPT6; deletion causes a vacuolar protein sorting defect
0.445	2.10E-162	YLR039C	RIC1	hom	Protein involved in retrograde transport to the cis-Golgi network; forms heterodimer with Rgp1p that acts as a GTP exchange factor for Ypt6p; involved in transcription of rRNA and ribosomal protein genes
0.377	1.35E-113	YNL051W	COG5	hom	Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
0.344	2.43E-93	YNL041C	COG6	hom	Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
0.326	9.37E-84	YML071C	COG8	hom	Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
0.314	1.36E-77	YDR320C	SWA2	hom	Auxilin-like protein involved in vesicular transport; clathrin-binding protein required for uncoating of clathrin-coated vesicles
0.307	1.00E-73	YKR019C	IRS4	hom	EH domain-containing protein; involved in regulating phosphatidylinositol 4,5-bisphosphate levels and autophagy; Irs4p and Tax4p bind and activate the PtdIns phosphatase Inp51p; Irs4p and Tax4p are involved in localizing Atg17p to the PAS; IRS4 has a paralog, TAX4, that arose from the whole genome duplication
0.275	7.09E-59	YGL005C	COG7	hom	Component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
0.214	6.40E-36	YGR167W	CLC1	hom	Clathrin light chain; subunit of the major coat protein involved in intracellular protein transport and endocytosis; thought to regulate clathrin function; two Clathrin heavy chains (CHC1) form the clathrin triskelion structural component; YGR167W
0.197	1.17E-30	YGL223C	COG1	hom	Essential component of the conserved oligomeric Golgi complex (Cog1p through Cog8p), a cytosolic tethering complex that functions in protein trafficking to mediate fusion of transport vesicles to Golgi compartments
0.178	2.45E-25	YKR020W	VPS51	hom	Component of the GARP (Golgi-associated retrograde protein) complex, Vps51p-Vps52p-Vps53p-Vps54p, which is required for the recycling of proteins from endosomes to the late Golgi; links the (VFT/GARP) complex to the SNARE Tlg1p
0.173	8.89E-24	YOL018C	TLG2	hom	Syntaxin-like t-SNARE that forms a complex with Tlg1p and Vti1p and mediates fusion of endosome-derived vesicles with the late Golgi; binds Vps45p, which prevents Tlg2p degradation and also facilitates t-SNARE complex formation; homologous to mammalian SNARE protein syntaxin 16 (Sx16)
0.169	8.87E-23	YJL004C	SYS1	hom	Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation