YDR150W / NUM1

Protein required for nuclear migration, localizes to the mother cell cortex and the bud tip; may mediate interactions of dynein and cytoplasmic microtubules with the cell cortex

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR150W / NUM1

Click on Significant Values for Screen Details ID:SGTC_44|Compound:0929-0055|FD-Score:4.70|P-value:5.95E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:8.66|P-value:6.74E-13 ID:SGTC_115|Compound:0527-0192|FD-Score:5.33|P-value:6.42E-6 ID:SGTC_138|Compound:0850-0259|FD-Score:6.43|P-value:6.83E-8 ID:SGTC_332|Compound:4112-0506|FD-Score:4.48|P-value:1.23E-4 ID:SGTC_393|Compound:acivicin|FD-Score:-3.95|P-value:6.04E-4 ID:SGTC_456|Compound:gingerol|FD-Score:3.93|P-value:6.41E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:4.28|P-value:2.31E-4 ID:SGTC_532|Compound:1548-0774|FD-Score:5.70|P-value:1.51E-6 ID:SGTC_575|Compound:k072-0246|FD-Score:7.00|P-value:4.99E-9 ID:SGTC_580|Compound:1123-0028|FD-Score:6.13|P-value:2.61E-7 ID:SGTC_596|Compound:1155-0152|FD-Score:4.95|P-value:2.56E-5 ID:SGTC_722|Compound:3534-0461|FD-Score:4.00|P-value:5.26E-4 ID:SGTC_736|Compound:0958-0254|FD-Score:4.62|P-value:7.74E-5 ID:SGTC_795|Compound:0685-0265|FD-Score:4.30|P-value:2.17E-4 ID:SGTC_905|Compound:2-aminofluorene|FD-Score:3.78|P-value:9.81E-4 ID:SGTC_1029|Compound:k015-0030|FD-Score:6.12|P-value:2.68E-7 ID:SGTC_1038|Compound:k292-0204|FD-Score:4.71|P-value:5.70E-5 ID:SGTC_1078|Compound:idebenone|FD-Score:5.81|P-value:9.73E-7 ID:SGTC_1082|Compound:camptothecin|FD-Score:4.79|P-value:4.41E-5 ID:SGTC_1105|Compound:epirubicin|FD-Score:3.91|P-value:6.87E-4 ID:SGTC_1195|Compound:1502-0147|FD-Score:4.26|P-value:2.41E-4 ID:SGTC_1250|Compound:0485-0349|FD-Score:3.84|P-value:8.22E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:4.43|P-value:1.41E-4 ID:SGTC_1367|Compound:1812-0510|FD-Score:4.26|P-value:2.41E-4 ID:SGTC_1406|Compound:3909-7906|FD-Score:5.51|P-value:3.20E-6 ID:SGTC_1534|Compound:6-azauridine|FD-Score:4.55|P-value:9.59E-5 ID:SGTC_1554|Compound:st069299|FD-Score:4.50|P-value:1.15E-4 ID:SGTC_1570|Compound:verbenone|FD-Score:3.85|P-value:8.08E-4 ID:SGTC_1573|Compound:oleylamine|FD-Score:4.67|P-value:6.58E-5 ID:SGTC_1677|Compound:st019204|FD-Score:-4.47|P-value:1.26E-4 ID:SGTC_1731|Compound:st035964|FD-Score:3.81|P-value:9.05E-4 ID:SGTC_1786|Compound:berberine|FD-Score:5.07|P-value:1.65E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.31|P-value:2.09E-4 ID:SGTC_2092|Compound:5303694|FD-Score:4.15|P-value:3.35E-4 ID:SGTC_2242|Compound:6630449|FD-Score:4.17|P-value:3.18E-4 ID:SGTC_2247|Compound:cerulenin|FD-Score:9.80|P-value:4.96E-16 ID:SGTC_2283|Compound:7944793|FD-Score:-5.71|P-value:1.45E-6 ID:SGTC_2296|Compound:7971656|FD-Score:5.38|P-value:5.25E-6 ID:SGTC_2320|Compound:7373003|FD-Score:4.33|P-value:1.95E-4 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:4.27|P-value:2.32E-4 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:4.56|P-value:9.35E-5 ID:SGTC_2710|Compound:st078562|FD-Score:-5.31|P-value:6.91E-6 ID:SGTC_2809|Compound:7964133|FD-Score:5.02|P-value:1.98E-5 ID:SGTC_3126|Compound:9127922|FD-Score:-3.84|P-value:8.30E-4 ID:SGTC_3129|Compound:9127257|FD-Score:-4.01|P-value:5.10E-4 ID:SGTC_3156|Compound:9099931|FD-Score:3.96|P-value:5.90E-4 ID:SGTC_3304|Compound:9125678|FD-Score:4.10|P-value:3.96E-4 ID:SGTC_44|Compound:0929-0055|FD-Score:4.70|P-value:5.95E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:8.66|P-value:6.74E-13 ID:SGTC_115|Compound:0527-0192|FD-Score:5.33|P-value:6.42E-6 ID:SGTC_138|Compound:0850-0259|FD-Score:6.43|P-value:6.83E-8 ID:SGTC_332|Compound:4112-0506|FD-Score:4.48|P-value:1.23E-4 ID:SGTC_393|Compound:acivicin|FD-Score:-3.95|P-value:6.04E-4 ID:SGTC_456|Compound:gingerol|FD-Score:3.93|P-value:6.41E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:4.28|P-value:2.31E-4 ID:SGTC_532|Compound:1548-0774|FD-Score:5.70|P-value:1.51E-6 ID:SGTC_575|Compound:k072-0246|FD-Score:7.00|P-value:4.99E-9 ID:SGTC_580|Compound:1123-0028|FD-Score:6.13|P-value:2.61E-7 ID:SGTC_596|Compound:1155-0152|FD-Score:4.95|P-value:2.56E-5 ID:SGTC_722|Compound:3534-0461|FD-Score:4.00|P-value:5.26E-4 ID:SGTC_736|Compound:0958-0254|FD-Score:4.62|P-value:7.74E-5 ID:SGTC_795|Compound:0685-0265|FD-Score:4.30|P-value:2.17E-4 ID:SGTC_905|Compound:2-aminofluorene|FD-Score:3.78|P-value:9.81E-4 ID:SGTC_1029|Compound:k015-0030|FD-Score:6.12|P-value:2.68E-7 ID:SGTC_1038|Compound:k292-0204|FD-Score:4.71|P-value:5.70E-5 ID:SGTC_1078|Compound:idebenone|FD-Score:5.81|P-value:9.73E-7 ID:SGTC_1082|Compound:camptothecin|FD-Score:4.79|P-value:4.41E-5 ID:SGTC_1105|Compound:epirubicin|FD-Score:3.91|P-value:6.87E-4 ID:SGTC_1195|Compound:1502-0147|FD-Score:4.26|P-value:2.41E-4 ID:SGTC_1250|Compound:0485-0349|FD-Score:3.84|P-value:8.22E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:4.43|P-value:1.41E-4 ID:SGTC_1367|Compound:1812-0510|FD-Score:4.26|P-value:2.41E-4 ID:SGTC_1406|Compound:3909-7906|FD-Score:5.51|P-value:3.20E-6 ID:SGTC_1534|Compound:6-azauridine|FD-Score:4.55|P-value:9.59E-5 ID:SGTC_1554|Compound:st069299|FD-Score:4.50|P-value:1.15E-4 ID:SGTC_1570|Compound:verbenone|FD-Score:3.85|P-value:8.08E-4 ID:SGTC_1573|Compound:oleylamine|FD-Score:4.67|P-value:6.58E-5 ID:SGTC_1677|Compound:st019204|FD-Score:-4.47|P-value:1.26E-4 ID:SGTC_1731|Compound:st035964|FD-Score:3.81|P-value:9.05E-4 ID:SGTC_1786|Compound:berberine|FD-Score:5.07|P-value:1.65E-5 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.31|P-value:2.09E-4 ID:SGTC_2092|Compound:5303694|FD-Score:4.15|P-value:3.35E-4 ID:SGTC_2242|Compound:6630449|FD-Score:4.17|P-value:3.18E-4 ID:SGTC_2247|Compound:cerulenin|FD-Score:9.80|P-value:4.96E-16 ID:SGTC_2283|Compound:7944793|FD-Score:-5.71|P-value:1.45E-6 ID:SGTC_2296|Compound:7971656|FD-Score:5.38|P-value:5.25E-6 ID:SGTC_2320|Compound:7373003|FD-Score:4.33|P-value:1.95E-4 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:4.27|P-value:2.32E-4 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:4.56|P-value:9.35E-5 ID:SGTC_2710|Compound:st078562|FD-Score:-5.31|P-value:6.91E-6 ID:SGTC_2809|Compound:7964133|FD-Score:5.02|P-value:1.98E-5 ID:SGTC_3126|Compound:9127922|FD-Score:-3.84|P-value:8.30E-4 ID:SGTC_3129|Compound:9127257|FD-Score:-4.01|P-value:5.10E-4 ID:SGTC_3156|Compound:9099931|FD-Score:3.96|P-value:5.90E-4 ID:SGTC_3304|Compound:9125678|FD-Score:4.10|P-value:3.96E-4

Top fitness defect scores for YDR150W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2247 cerulenin 9.80 830.0 nM 4.96E-16 1 5.58E-23
2 SGTC_58 0957-0393 8.66 9.0 µM 6.74E-13 cell wall 108 2.46E-18
3 SGTC_575 k072-0246 7.00 11.9 µM 4.99E-9 6 1.31E-12
4 SGTC_138 0850-0259 6.43 40.9 µM 6.83E-8 cell wall 93 6.23E-11
5 SGTC_580 1123-0028 6.13 41.0 µM 2.61E-7 DNA damage response 3 4.49E-10
6 SGTC_1029 k015-0030 6.12 64.7 µM 2.68E-7 5 4.68E-10
7 SGTC_1078 idebenone 5.81 36.1 µM 9.73E-7 Golgi 13 3.12E-9
8 SGTC_532 1548-0774 5.70 215.0 µM 1.51E-6 2 5.98E-9
9 SGTC_1406 3909-7906 5.51 11.9 µM 3.20E-6 60S ribosome export 1 1.80E-8
10 SGTC_2296 7971656 5.38 126.5 µM 5.25E-6 18 3.73E-8
11 SGTC_115 0527-0192 5.33 112.0 µM 6.42E-6 24 5.02E-8
12 SGTC_1786 berberine 5.07 21.6 µM 1.65E-5 60S ribosome export 16 2.01E-7
13 SGTC_2809 7964133 5.02 13.0 µM 1.98E-5 60S ribosome export 14 2.61E-7
14 SGTC_596 1155-0152 4.95 116.0 µM 2.56E-5 TRP & mitochondrial translation 16 3.81E-7
15 SGTC_1082 camptothecin 4.79 424.9 nM 4.41E-5 DNA damage response 35 8.47E-7
16 SGTC_1038 k292-0204 4.71 154.0 µM 5.70E-5 6 1.23E-6
17 SGTC_44 0929-0055 4.70 61.2 µM 5.95E-5 21 1.31E-6
18 SGTC_1573 oleylamine 4.67 1.1 µM 6.58E-5 15 1.52E-6
19 SGTC_736 0958-0254 4.62 92.6 µM 7.74E-5 Golgi 16 1.93E-6
20 SGTC_2697 mebhydrolin 4.56 72.4 µM 9.35E-5 NEO1 53 2.54E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.156 8.76E-20 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.146 2.07E-17 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.143 8.24E-17 YAL015C NTG1 hom DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
0.141 2.88E-16 YIL148W RPL40A hom Ubiquitin-ribosomal 60S subunit protein L40A fusion protein; cleaved to yield ubiquitin and ribosomal protein L40A; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; homologous to mammalian ribosomal protein L40, no bacterial homolog; RPL40A has a paralog, RPL40B, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.138 9.31E-16 YHR036W BRL1 het Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
0.136 2.59E-15 YPL269W KAR9 hom Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase
0.135 4.22E-15 YLR150W STM1 hom Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
0.132 1.39E-14 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.132 1.54E-14 YLL043W FPS1 hom Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress
0.131 2.77E-14 YKL115C_d YKL115C_d hom Dubious open reading frame, unlikely to encode a protein; partially overlaps the verified gene PRR1
0.130 4.43E-14 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.128 1.18E-13 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.127 1.42E-13 YMR152W YIM1 hom Protein of unknown function; null mutant displays sensitivity to DNA damaging agents; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; protein abundance increases in response to DNA replication stress
0.127 1.65E-13 YLR412W BER1 hom Protein involved in microtubule-related processes, N-acetylation; GFP-fusion protein localizes to the cytoplasm and is induced in response to the DNA-damaging agent MMS; YLR412W is not an essential gene; similar to Arabidopsis SRR1 gene
0.125 3.23E-13 YDR335W MSN5 hom Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm