YDR154C_d

Dubious open reading frame, null mutant exhibits synthetic phenotype with alpha-synuclein

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR154C / YDR154C

Click on Significant Values for Screen Details ID:SGTC_80|Compound:0159-0065|FD-Score:4.25|P-value:1.65E-6 ID:SGTC_217|Compound:ketoconazole|FD-Score:4.06|P-value:4.41E-6 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:-3.17|P-value:3.37E-4 ID:SGTC_249|Compound:wiskostatin|FD-Score:3.28|P-value:1.58E-4 ID:SGTC_265|Compound:mycophenolic acid|FD-Score:-3.31|P-value:1.96E-4 ID:SGTC_361|Compound:000s-0510|FD-Score:-3.70|P-value:3.59E-5 ID:SGTC_646|Compound:4092-0839|FD-Score:4.14|P-value:2.81E-6 ID:SGTC_654|Compound:1124-0271|FD-Score:2.85|P-value:8.48E-4 ID:SGTC_840|Compound:0245-0196|FD-Score:-2.97|P-value:7.48E-4 ID:SGTC_849|Compound:0386-0493|FD-Score:-3.44|P-value:1.13E-4 ID:SGTC_909|Compound:3448-9333|FD-Score:-3.04|P-value:5.61E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:2.88|P-value:7.62E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:3.75|P-value:1.98E-5 ID:SGTC_942|Compound:3253-1115|FD-Score:-3.63|P-value:4.79E-5 ID:SGTC_1068|Compound:loratadine|FD-Score:-3.24|P-value:2.61E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:4.09|P-value:3.66E-6 ID:SGTC_1170|Compound:0310-0127|FD-Score:3.21|P-value:2.09E-4 ID:SGTC_1171|Compound:0368-0077|FD-Score:5.44|P-value:1.39E-9 ID:SGTC_1184|Compound:1269-1718|FD-Score:-2.91|P-value:9.27E-4 ID:SGTC_1339|Compound:1486-1321|FD-Score:3.47|P-value:7.04E-5 ID:SGTC_1532|Compound:4',5'-dihydroxyflavone|FD-Score:3.14|P-value:2.77E-4 ID:SGTC_1573|Compound:oleylamine|FD-Score:3.76|P-value:1.88E-5 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:-3.01|P-value:6.37E-4 ID:SGTC_1594|Compound:piperine|FD-Score:-2.93|P-value:8.40E-4 ID:SGTC_1627|Compound:st003713|FD-Score:-3.04|P-value:5.73E-4 ID:SGTC_1632|Compound:st005650|FD-Score:-4.28|P-value:2.10E-6 ID:SGTC_1655|Compound:st012546|FD-Score:3.03|P-value:4.32E-4 ID:SGTC_1773|Compound:st048542|FD-Score:3.22|P-value:2.00E-4 ID:SGTC_1837|Compound:st055472|FD-Score:2.95|P-value:5.99E-4 ID:SGTC_2062|Compound:5245942|FD-Score:-2.93|P-value:8.41E-4 ID:SGTC_2147|Compound:5545932|FD-Score:3.57|P-value:4.47E-5 ID:SGTC_2274|Compound:7947094|FD-Score:-3.01|P-value:6.40E-4 ID:SGTC_2276|Compound:7947513|FD-Score:-3.03|P-value:5.84E-4 ID:SGTC_2398|Compound:5228213|FD-Score:3.87|P-value:1.12E-5 ID:SGTC_2483|Compound:5729050|FD-Score:2.96|P-value:5.75E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:3.57|P-value:4.56E-5 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:-3.21|P-value:2.86E-4 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:-4.21|P-value:2.96E-6 ID:SGTC_2630|Compound:antimycin a|FD-Score:3.13|P-value:2.85E-4 ID:SGTC_2816|Compound:7982706|FD-Score:3.65|P-value:3.08E-5 ID:SGTC_2823|Compound:7987624|FD-Score:3.30|P-value:1.48E-4 ID:SGTC_2841|Compound:9004761|FD-Score:-3.36|P-value:1.54E-4 ID:SGTC_2868|Compound:9035608|FD-Score:-2.98|P-value:7.00E-4 ID:SGTC_2901|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:-3.64|P-value:4.58E-5 ID:SGTC_2905|Compound:7799065|FD-Score:3.16|P-value:2.58E-4 ID:SGTC_2907|Compound:7721985|FD-Score:-3.49|P-value:8.97E-5 ID:SGTC_3138|Compound:9097216|FD-Score:3.03|P-value:4.29E-4 ID:SGTC_3141|Compound:9094609|FD-Score:-3.00|P-value:6.51E-4 ID:SGTC_3152|Compound:9099253|FD-Score:4.62|P-value:2.15E-7 ID:SGTC_3154|Compound:9100113|FD-Score:3.07|P-value:3.75E-4 ID:SGTC_3178|Compound:9107506|FD-Score:-3.16|P-value:3.57E-4 ID:SGTC_80|Compound:0159-0065|FD-Score:4.25|P-value:1.65E-6 ID:SGTC_217|Compound:ketoconazole|FD-Score:4.06|P-value:4.41E-6 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:-3.17|P-value:3.37E-4 ID:SGTC_249|Compound:wiskostatin|FD-Score:3.28|P-value:1.58E-4 ID:SGTC_265|Compound:mycophenolic acid|FD-Score:-3.31|P-value:1.96E-4 ID:SGTC_361|Compound:000s-0510|FD-Score:-3.70|P-value:3.59E-5 ID:SGTC_646|Compound:4092-0839|FD-Score:4.14|P-value:2.81E-6 ID:SGTC_654|Compound:1124-0271|FD-Score:2.85|P-value:8.48E-4 ID:SGTC_840|Compound:0245-0196|FD-Score:-2.97|P-value:7.48E-4 ID:SGTC_849|Compound:0386-0493|FD-Score:-3.44|P-value:1.13E-4 ID:SGTC_909|Compound:3448-9333|FD-Score:-3.04|P-value:5.61E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:2.88|P-value:7.62E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:3.75|P-value:1.98E-5 ID:SGTC_942|Compound:3253-1115|FD-Score:-3.63|P-value:4.79E-5 ID:SGTC_1068|Compound:loratadine|FD-Score:-3.24|P-value:2.61E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:4.09|P-value:3.66E-6 ID:SGTC_1170|Compound:0310-0127|FD-Score:3.21|P-value:2.09E-4 ID:SGTC_1171|Compound:0368-0077|FD-Score:5.44|P-value:1.39E-9 ID:SGTC_1184|Compound:1269-1718|FD-Score:-2.91|P-value:9.27E-4 ID:SGTC_1339|Compound:1486-1321|FD-Score:3.47|P-value:7.04E-5 ID:SGTC_1532|Compound:4',5'-dihydroxyflavone|FD-Score:3.14|P-value:2.77E-4 ID:SGTC_1573|Compound:oleylamine|FD-Score:3.76|P-value:1.88E-5 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:-3.01|P-value:6.37E-4 ID:SGTC_1594|Compound:piperine|FD-Score:-2.93|P-value:8.40E-4 ID:SGTC_1627|Compound:st003713|FD-Score:-3.04|P-value:5.73E-4 ID:SGTC_1632|Compound:st005650|FD-Score:-4.28|P-value:2.10E-6 ID:SGTC_1655|Compound:st012546|FD-Score:3.03|P-value:4.32E-4 ID:SGTC_1773|Compound:st048542|FD-Score:3.22|P-value:2.00E-4 ID:SGTC_1837|Compound:st055472|FD-Score:2.95|P-value:5.99E-4 ID:SGTC_2062|Compound:5245942|FD-Score:-2.93|P-value:8.41E-4 ID:SGTC_2147|Compound:5545932|FD-Score:3.57|P-value:4.47E-5 ID:SGTC_2274|Compound:7947094|FD-Score:-3.01|P-value:6.40E-4 ID:SGTC_2276|Compound:7947513|FD-Score:-3.03|P-value:5.84E-4 ID:SGTC_2398|Compound:5228213|FD-Score:3.87|P-value:1.12E-5 ID:SGTC_2483|Compound:5729050|FD-Score:2.96|P-value:5.75E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:3.57|P-value:4.56E-5 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:-3.21|P-value:2.86E-4 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:-4.21|P-value:2.96E-6 ID:SGTC_2630|Compound:antimycin a|FD-Score:3.13|P-value:2.85E-4 ID:SGTC_2816|Compound:7982706|FD-Score:3.65|P-value:3.08E-5 ID:SGTC_2823|Compound:7987624|FD-Score:3.30|P-value:1.48E-4 ID:SGTC_2841|Compound:9004761|FD-Score:-3.36|P-value:1.54E-4 ID:SGTC_2868|Compound:9035608|FD-Score:-2.98|P-value:7.00E-4 ID:SGTC_2901|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:-3.64|P-value:4.58E-5 ID:SGTC_2905|Compound:7799065|FD-Score:3.16|P-value:2.58E-4 ID:SGTC_2907|Compound:7721985|FD-Score:-3.49|P-value:8.97E-5 ID:SGTC_3138|Compound:9097216|FD-Score:3.03|P-value:4.29E-4 ID:SGTC_3141|Compound:9094609|FD-Score:-3.00|P-value:6.51E-4 ID:SGTC_3152|Compound:9099253|FD-Score:4.62|P-value:2.15E-7 ID:SGTC_3154|Compound:9100113|FD-Score:3.07|P-value:3.75E-4 ID:SGTC_3178|Compound:9107506|FD-Score:-3.16|P-value:3.57E-4

Top fitness defect scores for YDR154C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1171 0368-0077 5.44 21.4 µM 1.39E-9 6 2.70E-8
2 SGTC_3152 9099253 4.62 49.5 µM 2.15E-7 NEO1-PIK1 98 1.93E-6
3 SGTC_80 0159-0065 4.25 512.2 µM 1.65E-6 2 1.09E-5
4 SGTC_646 4092-0839 4.14 27.3 µM 2.81E-6 plasma membrane duress 24 1.71E-5
5 SGTC_1072 oxiconazole 4.09 14.9 nM 3.66E-6 ergosterol depletion effects on membrane 48 2.14E-5
6 SGTC_217 ketoconazole 4.06 989.0 nM 4.41E-6 ergosterol depletion effects on membrane 23 2.50E-5
7 SGTC_2398 5228213 3.87 48.8 µM 1.12E-5 iron homeostasis 42 5.52E-5
8 SGTC_1573 oleylamine 3.76 1.1 µM 1.88E-5 33 8.55E-5
9 SGTC_933 2914-0560 3.75 114.0 µM 1.98E-5 29 8.95E-5
10 SGTC_2816 7982706 3.65 58.4 µM 3.08E-5 azole & statin 66 1.30E-4
11 SGTC_2147 5545932 3.57 89.7 µM 4.47E-5 ergosterol biosynthesis 22 1.78E-4
12 SGTC_2533 agelasine 3.57 13.7 µM 4.56E-5 ERG2 61 1.81E-4
13 SGTC_1339 1486-1321 3.47 22.7 µM 7.04E-5 ERG2 41 2.62E-4
14 SGTC_2823 7987624 3.30 58.4 µM 1.48E-4 67 4.91E-4
15 SGTC_249 wiskostatin 3.28 11.2 µM 1.58E-4 31 5.20E-4
16 SGTC_1773 st048542 3.22 73.6 µM 2.00E-4 82 6.36E-4
17 SGTC_1170 0310-0127 3.21 64.8 µM 2.09E-4 55 6.61E-4
18 SGTC_2905 7799065 3.16 20.1 µM 2.58E-4 31 7.89E-4
19 SGTC_1532 4',5'-dihydroxyflavone 3.14 78.7 µM 2.77E-4 Golgi 43 8.38E-4
20 SGTC_2630 antimycin a 3.13 100.0 µM 2.85E-4 mitochondrial processes 75 8.60E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.111 1.20E-10 YMR071C TVP18 hom Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments
0.097 1.75E-8 YHR086W NAM8 hom RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function
0.096 2.32E-8 YGL233W SEC15 het Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase
0.093 5.77E-8 YDR189W SLY1 het Hydrophilic protein involved in vesicle trafficking between the ER and Golgi; SM (Sec1/Munc-18) family protein that binds the tSNARE Sed5p and stimulates its assembly into a trans-SNARE membrane-protein complex
0.092 8.82E-8 YCR032W BPH1 hom Protein homologous to Chediak-Higashi syndrome and Beige proteins; both of which are implicated in disease syndromes in human and mouse, respectively, due to defective lysosomal trafficking; mutant phenotype and genetic interactions suggest a role in protein sorting
0.091 1.18E-7 YNL216W RAP1 het Essential DNA-binding transcription regulator that binds at many loci; involved in either transcription activation or repression, chromatin silencing, and telomere length maintenance; conserved protein with an N-terminal BRCT domain, a central region with homology to the Myb DNA binding domain, and a C-terminal Rap1-specific protein-interaction domain (RCT domain)
0.084 9.76E-7 YLR453C RIF2 hom Protein that binds to the Rap1p C-terminus and acts synergistically with Rif1p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation
0.084 1.19E-6 YOL108C INO4 hom Transcription factor required for derepression of inositol-choline-regulated genes involved in phospholipid synthesis; forms a complex, with Ino2p, that binds the inositol-choline-responsive element through a basic helix-loop-helix domain
0.083 1.37E-6 YMR173W DDR48 hom DNA damage-responsive protein; expression is increased in response to heat-shock stress or treatments that produce DNA lesions; contains multiple repeats of the amino acid sequence NNNDSYGS; protein abundance increases in response to DNA replication stress
0.080 3.26E-6 YGL045W RIM8 hom Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family
0.077 7.60E-6 YMR042W ARG80 hom Transcription factor involved in regulation of arginine-responsive genes; acts with Arg81p and Arg82p
0.077 7.24E-6 YMR196W_p YMR196W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YMR196W is not an essential gene
0.077 7.63E-6 YOL084W PHM7 hom Protein of unknown function; expression is regulated by phosphate levels; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery and vacuole; protein abundance increases in response to DNA replication stress
0.077 8.58E-6 YEL058W PCM1 het Essential N-acetylglucosamine-phosphate mutase; converts GlcNAc-6-P to GlcNAc-1-P, which is a precursor for the biosynthesis of chitin and for the formation of N-glycosylated mannoproteins and glycosylphosphatidylinositol anchors
0.076 1.04E-5 YBR089C-A NHP6B hom High-mobility group (HMG) protein, binds to and remodels nucleosomes; involved in recruiting FACT and other chromatin remodelling complexes to the chromosomes; functionally redundant with Nhp6Ap; homologous to mammalian HMGB1 and HMGB2; NHP6B has a paralog, NHP6A, that arose from the whole genome duplication