YDR165W / TRM82

Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR165W / TRM82

Click on Significant Values for Screen Details ID:SGTC_56|Compound:0957-0365|FD-Score:-3.83|P-value:6.09E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.23|P-value:5.77E-4 ID:SGTC_61|Compound:0978-8327|FD-Score:-3.21|P-value:6.28E-4 ID:SGTC_63|Compound:0099-0242|FD-Score:-3.35|P-value:3.88E-4 ID:SGTC_249|Compound:wiskostatin|FD-Score:-3.36|P-value:3.75E-4 ID:SGTC_271|Compound:rotenone|FD-Score:3.25|P-value:8.59E-4 ID:SGTC_318|Compound:1488-0629|FD-Score:8.75|P-value:6.20E-18 ID:SGTC_427|Compound:1354-0053|FD-Score:3.27|P-value:8.01E-4 ID:SGTC_555|Compound:4296-0685|FD-Score:3.76|P-value:1.38E-4 ID:SGTC_612|Compound:0302-0167|FD-Score:4.34|P-value:1.30E-5 ID:SGTC_884|Compound:0851-0618|FD-Score:-3.63|P-value:1.37E-4 ID:SGTC_921|Compound:1561-0023|FD-Score:3.42|P-value:4.83E-4 ID:SGTC_1039|Compound:k821-0043|FD-Score:-3.54|P-value:1.88E-4 ID:SGTC_1220|Compound:1838-0075|FD-Score:-3.93|P-value:4.16E-5 ID:SGTC_1230|Compound:0170-0365|FD-Score:-3.23|P-value:5.91E-4 ID:SGTC_1535|Compound:tetrahydropapaveroline hydrobromide|FD-Score:-3.21|P-value:6.34E-4 ID:SGTC_1816|Compound:st051957|FD-Score:3.71|P-value:1.65E-4 ID:SGTC_1938|Compound:st074717|FD-Score:-5.04|P-value:2.45E-7 ID:SGTC_1946|Compound:st076057|FD-Score:-4.12|P-value:1.85E-5 ID:SGTC_1948|Compound:st076329|FD-Score:-5.84|P-value:2.96E-9 ID:SGTC_2016|Compound:5121758|FD-Score:3.32|P-value:6.89E-4 ID:SGTC_2175|Compound:5807670|FD-Score:-4.54|P-value:2.87E-6 ID:SGTC_2176|Compound:5809774|FD-Score:-5.69|P-value:6.97E-9 ID:SGTC_2421|Compound:5690543|FD-Score:-3.15|P-value:7.72E-4 ID:SGTC_2430|Compound:5571206|FD-Score:-3.82|P-value:6.43E-5 ID:SGTC_2523|Compound:spermine|FD-Score:-3.26|P-value:5.32E-4 ID:SGTC_2524|Compound:avocatin b|FD-Score:-3.30|P-value:4.61E-4 ID:SGTC_2550|Compound:chrysin dimethyl ether|FD-Score:-3.34|P-value:3.96E-4 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:-3.11|P-value:8.85E-4 ID:SGTC_2693|Compound:st077581|FD-Score:-3.45|P-value:2.70E-4 ID:SGTC_2708|Compound:st078559|FD-Score:-3.67|P-value:1.15E-4 ID:SGTC_2716|Compound:st080858|FD-Score:-3.29|P-value:4.76E-4 ID:SGTC_2717|Compound:st081029|FD-Score:-3.41|P-value:3.11E-4 ID:SGTC_2762|Compound:leflunomide|FD-Score:5.25|P-value:1.69E-7 ID:SGTC_56|Compound:0957-0365|FD-Score:-3.83|P-value:6.09E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.23|P-value:5.77E-4 ID:SGTC_61|Compound:0978-8327|FD-Score:-3.21|P-value:6.28E-4 ID:SGTC_63|Compound:0099-0242|FD-Score:-3.35|P-value:3.88E-4 ID:SGTC_249|Compound:wiskostatin|FD-Score:-3.36|P-value:3.75E-4 ID:SGTC_271|Compound:rotenone|FD-Score:3.25|P-value:8.59E-4 ID:SGTC_318|Compound:1488-0629|FD-Score:8.75|P-value:6.20E-18 ID:SGTC_427|Compound:1354-0053|FD-Score:3.27|P-value:8.01E-4 ID:SGTC_555|Compound:4296-0685|FD-Score:3.76|P-value:1.38E-4 ID:SGTC_612|Compound:0302-0167|FD-Score:4.34|P-value:1.30E-5 ID:SGTC_884|Compound:0851-0618|FD-Score:-3.63|P-value:1.37E-4 ID:SGTC_921|Compound:1561-0023|FD-Score:3.42|P-value:4.83E-4 ID:SGTC_1039|Compound:k821-0043|FD-Score:-3.54|P-value:1.88E-4 ID:SGTC_1220|Compound:1838-0075|FD-Score:-3.93|P-value:4.16E-5 ID:SGTC_1230|Compound:0170-0365|FD-Score:-3.23|P-value:5.91E-4 ID:SGTC_1535|Compound:tetrahydropapaveroline hydrobromide|FD-Score:-3.21|P-value:6.34E-4 ID:SGTC_1816|Compound:st051957|FD-Score:3.71|P-value:1.65E-4 ID:SGTC_1938|Compound:st074717|FD-Score:-5.04|P-value:2.45E-7 ID:SGTC_1946|Compound:st076057|FD-Score:-4.12|P-value:1.85E-5 ID:SGTC_1948|Compound:st076329|FD-Score:-5.84|P-value:2.96E-9 ID:SGTC_2016|Compound:5121758|FD-Score:3.32|P-value:6.89E-4 ID:SGTC_2175|Compound:5807670|FD-Score:-4.54|P-value:2.87E-6 ID:SGTC_2176|Compound:5809774|FD-Score:-5.69|P-value:6.97E-9 ID:SGTC_2421|Compound:5690543|FD-Score:-3.15|P-value:7.72E-4 ID:SGTC_2430|Compound:5571206|FD-Score:-3.82|P-value:6.43E-5 ID:SGTC_2523|Compound:spermine|FD-Score:-3.26|P-value:5.32E-4 ID:SGTC_2524|Compound:avocatin b|FD-Score:-3.30|P-value:4.61E-4 ID:SGTC_2550|Compound:chrysin dimethyl ether|FD-Score:-3.34|P-value:3.96E-4 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:-3.11|P-value:8.85E-4 ID:SGTC_2693|Compound:st077581|FD-Score:-3.45|P-value:2.70E-4 ID:SGTC_2708|Compound:st078559|FD-Score:-3.67|P-value:1.15E-4 ID:SGTC_2716|Compound:st080858|FD-Score:-3.29|P-value:4.76E-4 ID:SGTC_2717|Compound:st081029|FD-Score:-3.41|P-value:3.11E-4 ID:SGTC_2762|Compound:leflunomide|FD-Score:5.25|P-value:1.69E-7

Top fitness defect scores for YDR165W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_318 1488-0629 8.75 67.2 µM 6.20E-18 copper-dependent oxidative stress 3 1.09E-18
2 SGTC_2762 leflunomide 5.25 42.1 µM 1.69E-7 8 7.75E-8
3 SGTC_612 0302-0167 4.34 22.4 µM 1.30E-5 copper-dependent oxidative stress 28 7.23E-6
4 SGTC_555 4296-0685 3.76 37.0 µM 1.38E-4 ergosterol biosynthesis 12 8.52E-5
5 SGTC_1816 st051957 3.71 91.6 µM 1.65E-4 13 1.03E-4
6 SGTC_921 1561-0023 3.42 372.0 µM 4.83E-4 37 3.16E-4
7 SGTC_2016 5121758 3.32 140.0 µM 6.89E-4 redox potentiating 83 4.58E-4
8 SGTC_427 1354-0053 3.27 47.3 µM 8.01E-4 48 5.36E-4
9 SGTC_271 rotenone 3.25 740.0 µM 8.59E-4 56 5.77E-4
10 SGTC_1821 st053465 3.18 65.6 µM 0.00109 27 7.42E-4
11 SGTC_2364 9055074 3.18 200.0 µM 0.00109 47 7.43E-4
12 SGTC_1655 st012546 3.18 55.1 µM 0.00109 54 7.43E-4
13 SGTC_1747 st037496 3.12 66.6 µM 0.00130 51 8.90E-4
14 SGTC_3005 9076496 3.09 71.4 µM 0.00146 25 0.00100
15 SGTC_1648 st011736 3.06 69.8 µM 0.00158 PDR1 45 0.00110
16 SGTC_2017 5132927 3.06 60.6 µM 0.00160 77 0.00111
17 SGTC_1588 farnesol 3.05 29.9 µM 0.00166 44 0.00115
18 SGTC_1660 st012960 2.99 28.1 µM 0.00197 iron homeostasis 89 0.00137
19 SGTC_2343 7990716 2.96 14.8 µM 0.00220 55 0.00154
20 SGTC_2992 9074591 2.93 71.4 µM 0.00239 43 0.00169

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.282 2.74E-62 YDL201W TRM8 hom Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p
0.172 8.77E-24 YGR110W CLD1 hom Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58
0.169 5.97E-23 YGL152C_d YGL152C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF PEX14/YGL153W
0.166 3.01E-22 YDR143C SAN1 hom Ubiquitin-protein ligase; involved in the proteasome-dependent degradation of aberrant nuclear proteins; targets substrates with regions of exposed hydrophobicity containing 5 or more contiguous hydrophobic residues; contains intrinsically disordered regions that contribute to substrate recognition
0.160 9.15E-21 YDR452W PPN1 hom Vacuolar endopolyphosphatase with a role in phosphate metabolism; functions as a homodimer; relocalizes from vacuole to cytoplasm upon DNA replication stress
0.155 1.40E-19 YDR206W EBS1 hom Protein involved in inhibition of translation and nonsense-mediated decay; interacts with cap binding protein Cdc33p and with Nam7p; localizes to P-bodies upon glucose starvation; mRNA abundance regulated by mRNA decay factors
0.147 9.84E-18 YGL232W TAN1 hom Putative tRNA acetyltransferase; RNA-binding protein required for the formation of the modified nucleoside N(4)-acetylcytidine in serine and leucine tRNAs but not required for the same modification in 18S rRNA; protein abundance increases in response to DNA replication stress
0.144 5.96E-17 YMR080C NAM7 hom ATP-dependent RNA helicase of the SFI superfamily; involved in nonsense mediated mRNA decay; required for efficient translation termination at nonsense codons and targeting of NMD substrates to P-bodies; involved in telomere maintenance; forms cytoplasmic foci upon DNA replication stress
0.143 1.04E-16 YGR096W TPC1 hom Mitochondrial membrane transporter that mediates uptake of the essential cofactor thiamine pyrophosphate (ThPP) into mitochondria; expression appears to be regulated by carbon source; member of the mitochondrial carrier family
0.137 1.38E-15 YDR198C RKM2 hom Ribosomal protein lysine methyltransferase, responsible for trimethylation of the lysine residue at position 3 of Rpl12Ap and Rpl12Bp
0.135 3.95E-15 YAL066W_d YAL066W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.131 2.70E-14 YLR328W NMA1 hom Nicotinic acid mononucleotide adenylyltransferase; catalyzes the transfer of the adenylyl moiety of ATP to nicotinamide mononucleotide to form NAD; involved in pathways of NAD biosynthesis, including the de novo, NAD(+) salvage, and nicotinamide riboside salvage pathways; NMA1 has a paralog, NMA2, that arose from the whole genome duplication
0.130 4.01E-14 YDR223W CRF1 hom Transcriptional corepressor involved in repression of ribosomal protein (RP) gene transcription via the TOR signaling pathway which promotes accumulation of Crf1p in the nucleus; role in repression of RP genes varies by strain
0.128 1.09E-13 YHL024W RIM4 hom Putative RNA-binding protein required for the expression of early and middle sporulation genes
0.105 1.06E-9 YOR308C SNU66 hom Component of the U4/U6.U5 snRNP complex involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1