YDR166C / SEC5

Essential 107kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in assembly of the exocyst complex; required with Sec3p for ER inheritance where it promotes anchoring of the cortical ER at the bud tip

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR166C / SEC5

Click on Significant Values for Screen Details ID:SGTC_57|Compound:miconazole|FD-Score:6.83|P-value:3.64E-13 ID:SGTC_67|Compound:0335-0847|FD-Score:4.04|P-value:1.12E-5 ID:SGTC_73|Compound:0327-0325|FD-Score:-4.31|P-value:2.62E-6 ID:SGTC_160|Compound:3453-0671|FD-Score:-4.12|P-value:6.58E-6 ID:SGTC_187|Compound:1488-2104|FD-Score:-3.25|P-value:2.93E-4 ID:SGTC_193|Compound:1273-0060|FD-Score:4.87|P-value:1.57E-7 ID:SGTC_204|Compound:1543-0184|FD-Score:5.20|P-value:2.37E-8 ID:SGTC_205|Compound:1486-1330|FD-Score:3.19|P-value:4.18E-4 ID:SGTC_206|Compound:4546-0025|FD-Score:5.54|P-value:3.06E-9 ID:SGTC_207|Compound:4185-0010|FD-Score:6.04|P-value:1.11E-10 ID:SGTC_252|Compound:miconazole|FD-Score:3.03|P-value:7.55E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:3.37|P-value:2.04E-4 ID:SGTC_306|Compound:1672-3436|FD-Score:3.22|P-value:3.72E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:8.02|P-value:1.67E-17 ID:SGTC_320|Compound:4092-0846|FD-Score:5.83|P-value:4.53E-10 ID:SGTC_685|Compound:1278-0033|FD-Score:4.06|P-value:1.01E-5 ID:SGTC_757|Compound:1066-0008|FD-Score:3.03|P-value:7.52E-4 ID:SGTC_767|Compound:0929-0023|FD-Score:3.39|P-value:1.89E-4 ID:SGTC_773|Compound:4358-1457|FD-Score:5.47|P-value:4.57E-9 ID:SGTC_815|Compound:1582-0027|FD-Score:3.24|P-value:3.36E-4 ID:SGTC_874|Compound:0599-0792|FD-Score:3.25|P-value:3.26E-4 ID:SGTC_892|Compound:0929-0076|FD-Score:3.17|P-value:4.47E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:-3.20|P-value:3.49E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:-4.74|P-value:2.77E-7 ID:SGTC_1042|Compound:0350-0096|FD-Score:3.44|P-value:1.54E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:4.42|P-value:1.78E-6 ID:SGTC_1204|Compound:4049-0315|FD-Score:3.16|P-value:4.72E-4 ID:SGTC_1237|Compound:0330-0133|FD-Score:3.76|P-value:3.96E-5 ID:SGTC_1280|Compound:0880-0253|FD-Score:-2.94|P-value:9.35E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:3.19|P-value:4.22E-4 ID:SGTC_1289|Compound:0976-0073|FD-Score:3.08|P-value:6.34E-4 ID:SGTC_1470|Compound:k295-0292|FD-Score:2.97|P-value:9.37E-4 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:3.23|P-value:3.53E-4 ID:SGTC_1732|Compound:st034308|FD-Score:-2.99|P-value:7.77E-4 ID:SGTC_1737|Compound:st035557|FD-Score:-3.12|P-value:4.82E-4 ID:SGTC_1828|Compound:st055811|FD-Score:-3.11|P-value:5.05E-4 ID:SGTC_1910|Compound:st062352|FD-Score:3.30|P-value:2.75E-4 ID:SGTC_2036|Compound:5160441|FD-Score:3.19|P-value:4.16E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.58|P-value:7.71E-7 ID:SGTC_2294|Compound:7971431|FD-Score:3.43|P-value:1.60E-4 ID:SGTC_2356|Compound:9061756|FD-Score:3.26|P-value:3.19E-4 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:4.24|P-value:4.40E-6 ID:SGTC_2533|Compound:agelasine|FD-Score:-2.99|P-value:7.83E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.51|P-value:1.03E-4 ID:SGTC_2587|Compound:piperic acid|FD-Score:3.34|P-value:2.30E-4 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:3.04|P-value:7.21E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:3.54|P-value:1.05E-4 ID:SGTC_2896|Compound:9052656|FD-Score:3.38|P-value:1.98E-4 ID:SGTC_2910|Compound:7948405|FD-Score:3.16|P-value:4.63E-4 ID:SGTC_2934|Compound:9036370|FD-Score:-4.68|P-value:3.91E-7 ID:SGTC_2935|Compound:9038016|FD-Score:-3.24|P-value:3.06E-4 ID:SGTC_2942|Compound:9052777|FD-Score:3.07|P-value:6.53E-4 ID:SGTC_2944|Compound:9067346|FD-Score:-3.07|P-value:5.74E-4 ID:SGTC_2978|Compound:9011784|FD-Score:-3.28|P-value:2.59E-4 ID:SGTC_3030|Compound:9087619|FD-Score:-2.99|P-value:7.76E-4 ID:SGTC_3035|Compound:9091303|FD-Score:3.22|P-value:3.74E-4 ID:SGTC_3037|Compound:9091026|FD-Score:3.85|P-value:2.72E-5 ID:SGTC_3118|Compound:9124250|FD-Score:-3.01|P-value:7.31E-4 ID:SGTC_3143|Compound:9086658|FD-Score:2.96|P-value:9.71E-4 ID:SGTC_3205|Compound:9113483|FD-Score:-3.91|P-value:1.80E-5 ID:SGTC_3206|Compound:9114350|FD-Score:3.28|P-value:2.89E-4 ID:SGTC_3211|Compound:9128781|FD-Score:4.99|P-value:8.16E-8 ID:SGTC_3266|Compound:9136860|FD-Score:-3.90|P-value:1.87E-5 ID:SGTC_3326|Compound:9140064|FD-Score:3.32|P-value:2.50E-4 ID:SGTC_57|Compound:miconazole|FD-Score:6.83|P-value:3.64E-13 ID:SGTC_67|Compound:0335-0847|FD-Score:4.04|P-value:1.12E-5 ID:SGTC_73|Compound:0327-0325|FD-Score:-4.31|P-value:2.62E-6 ID:SGTC_160|Compound:3453-0671|FD-Score:-4.12|P-value:6.58E-6 ID:SGTC_187|Compound:1488-2104|FD-Score:-3.25|P-value:2.93E-4 ID:SGTC_193|Compound:1273-0060|FD-Score:4.87|P-value:1.57E-7 ID:SGTC_204|Compound:1543-0184|FD-Score:5.20|P-value:2.37E-8 ID:SGTC_205|Compound:1486-1330|FD-Score:3.19|P-value:4.18E-4 ID:SGTC_206|Compound:4546-0025|FD-Score:5.54|P-value:3.06E-9 ID:SGTC_207|Compound:4185-0010|FD-Score:6.04|P-value:1.11E-10 ID:SGTC_252|Compound:miconazole|FD-Score:3.03|P-value:7.55E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:3.37|P-value:2.04E-4 ID:SGTC_306|Compound:1672-3436|FD-Score:3.22|P-value:3.72E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:8.02|P-value:1.67E-17 ID:SGTC_320|Compound:4092-0846|FD-Score:5.83|P-value:4.53E-10 ID:SGTC_685|Compound:1278-0033|FD-Score:4.06|P-value:1.01E-5 ID:SGTC_757|Compound:1066-0008|FD-Score:3.03|P-value:7.52E-4 ID:SGTC_767|Compound:0929-0023|FD-Score:3.39|P-value:1.89E-4 ID:SGTC_773|Compound:4358-1457|FD-Score:5.47|P-value:4.57E-9 ID:SGTC_815|Compound:1582-0027|FD-Score:3.24|P-value:3.36E-4 ID:SGTC_874|Compound:0599-0792|FD-Score:3.25|P-value:3.26E-4 ID:SGTC_892|Compound:0929-0076|FD-Score:3.17|P-value:4.47E-4 ID:SGTC_946|Compound:3434-1334|FD-Score:-3.20|P-value:3.49E-4 ID:SGTC_951|Compound:1171-0579|FD-Score:-4.74|P-value:2.77E-7 ID:SGTC_1042|Compound:0350-0096|FD-Score:3.44|P-value:1.54E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:4.42|P-value:1.78E-6 ID:SGTC_1204|Compound:4049-0315|FD-Score:3.16|P-value:4.72E-4 ID:SGTC_1237|Compound:0330-0133|FD-Score:3.76|P-value:3.96E-5 ID:SGTC_1280|Compound:0880-0253|FD-Score:-2.94|P-value:9.35E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:3.19|P-value:4.22E-4 ID:SGTC_1289|Compound:0976-0073|FD-Score:3.08|P-value:6.34E-4 ID:SGTC_1470|Compound:k295-0292|FD-Score:2.97|P-value:9.37E-4 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:3.23|P-value:3.53E-4 ID:SGTC_1732|Compound:st034308|FD-Score:-2.99|P-value:7.77E-4 ID:SGTC_1737|Compound:st035557|FD-Score:-3.12|P-value:4.82E-4 ID:SGTC_1828|Compound:st055811|FD-Score:-3.11|P-value:5.05E-4 ID:SGTC_1910|Compound:st062352|FD-Score:3.30|P-value:2.75E-4 ID:SGTC_2036|Compound:5160441|FD-Score:3.19|P-value:4.16E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:4.58|P-value:7.71E-7 ID:SGTC_2294|Compound:7971431|FD-Score:3.43|P-value:1.60E-4 ID:SGTC_2356|Compound:9061756|FD-Score:3.26|P-value:3.19E-4 ID:SGTC_2530|Compound:3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene|FD-Score:4.24|P-value:4.40E-6 ID:SGTC_2533|Compound:agelasine|FD-Score:-2.99|P-value:7.83E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.51|P-value:1.03E-4 ID:SGTC_2587|Compound:piperic acid|FD-Score:3.34|P-value:2.30E-4 ID:SGTC_2597|Compound:laccaic acid a|FD-Score:3.04|P-value:7.21E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:3.54|P-value:1.05E-4 ID:SGTC_2896|Compound:9052656|FD-Score:3.38|P-value:1.98E-4 ID:SGTC_2910|Compound:7948405|FD-Score:3.16|P-value:4.63E-4 ID:SGTC_2934|Compound:9036370|FD-Score:-4.68|P-value:3.91E-7 ID:SGTC_2935|Compound:9038016|FD-Score:-3.24|P-value:3.06E-4 ID:SGTC_2942|Compound:9052777|FD-Score:3.07|P-value:6.53E-4 ID:SGTC_2944|Compound:9067346|FD-Score:-3.07|P-value:5.74E-4 ID:SGTC_2978|Compound:9011784|FD-Score:-3.28|P-value:2.59E-4 ID:SGTC_3030|Compound:9087619|FD-Score:-2.99|P-value:7.76E-4 ID:SGTC_3035|Compound:9091303|FD-Score:3.22|P-value:3.74E-4 ID:SGTC_3037|Compound:9091026|FD-Score:3.85|P-value:2.72E-5 ID:SGTC_3118|Compound:9124250|FD-Score:-3.01|P-value:7.31E-4 ID:SGTC_3143|Compound:9086658|FD-Score:2.96|P-value:9.71E-4 ID:SGTC_3205|Compound:9113483|FD-Score:-3.91|P-value:1.80E-5 ID:SGTC_3206|Compound:9114350|FD-Score:3.28|P-value:2.89E-4 ID:SGTC_3211|Compound:9128781|FD-Score:4.99|P-value:8.16E-8 ID:SGTC_3266|Compound:9136860|FD-Score:-3.90|P-value:1.87E-5 ID:SGTC_3326|Compound:9140064|FD-Score:3.32|P-value:2.50E-4

Top fitness defect scores for YDR166C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_312 4092-0653 8.02 6.8 µM 1.67E-17 ergosterol depletion effects on membrane 8 5.08E-16 0.06
2 SGTC_57 miconazole 6.83 162.5 nM 3.64E-13 ergosterol depletion effects on membrane 13 4.28E-12 0.52
3 SGTC_207 4185-0010 6.04 4.5 µM 1.11E-10 endomembrane recycling 9 7.62E-10 0.33
4 SGTC_320 4092-0846 5.83 5.3 µM 4.53E-10 ergosterol depletion effects on membrane 11 2.73E-9 0.23
5 SGTC_206 4546-0025 5.54 72.0 µM 3.06E-9 NEO1 6 1.54E-8 0.24
6 SGTC_773 4358-1457 5.47 44.6 µM 4.57E-9 6 2.22E-8 0.35
7 SGTC_204 1543-0184 5.20 62.4 µM 2.37E-8 TRP & mitochondrial translation 4 9.87E-8 0.16
8 SGTC_3211 9128781 4.99 49.5 µM 8.16E-8 9 3.03E-7 0.05
9 SGTC_193 1273-0060 4.87 1.6 µM 1.57E-7 ergosterol biosynthesis 4 5.50E-7 0.64
10 SGTC_2249 haloperidol 4.58 116.4 µM 7.71E-7 ergosterol depletion effects on membrane 28 2.33E-6 0.15
11 SGTC_1072 oxiconazole 4.42 14.9 nM 1.78E-6 ergosterol depletion effects on membrane 15 4.99E-6 0.11
12 SGTC_2530 3-alpha-hydroxy-4,4-bisnor-8,11,13-podocarpatriene 4.24 92.6 µM 4.40E-6 10 1.14E-5 0.39
13 SGTC_685 1278-0033 4.06 54.9 µM 1.01E-5 60S ribosome export 9 2.42E-5 0.42
14 SGTC_67 0335-0847 4.04 266.0 µM 1.12E-5 ubiquinone biosynthesis & proteasome 20 2.66E-5 0.01
15 SGTC_3037 9091026 3.85 49.5 µM 2.72E-5 NEO1-PIK1 22 5.95E-5 0.06
16 SGTC_1237 0330-0133 3.76 46.1 µM 3.96E-5 ergosterol biosynthesis 11 8.37E-5 0.04
17 SGTC_2778 tioconazole 3.54 65.1 nM 1.05E-4 ergosterol depletion effects on membrane 37 2.03E-4 0.03
18 SGTC_1042 0350-0096 3.44 82.0 µM 1.54E-4 TSC3-RPN4 9 2.89E-4 0.08
19 SGTC_2294 7971431 3.43 200.0 µM 1.60E-4 6 2.98E-4 0.08
20 SGTC_767 0929-0023 3.39 86.0 µM 1.89E-4 TRP & mitochondrial translation 19 3.48E-4 0.08

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.334 4.47E-88 YIL068C SEC6 het Essential 88kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; anchors the assembled complex to sites of secretion; interacts with SM-like protein and SNARE regulator Sec1p and may recruit it to sites of secretion; interacts with Sec9p and inhibits formation of the t-SNARE complex between Sec9p and Sso1p
0.308 1.82E-74 YFL034W_p YFL034W_p hom Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk
0.304 1.29E-72 YPR055W SEC8 het Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress
0.299 2.19E-70 YGL233W SEC15 het Essential 113 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; interacts with and functions as a downstream effector of active, GTP-bound Sec4p, a Rab family GTPase
0.297 3.29E-69 YBR102C EXO84 het Exocyst subunit with dual roles in exocytosis and spliceosome assembly; subunit of the the exocyst complex which mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane (PM) prior to SNARE-mediated fusion; required for exocyst assembly and targeting the complex to specific sites on the bud tip PM; associates the U1 snRNP; role in pre-mRNA splicing and prespliceosome formation; possible Cdc28 substrate
0.296 5.04E-69 YBL010C_p YBL010C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein colocalizes with clathrin-coated vesicles
0.271 1.71E-57 YMR218C TRS130 het One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy
0.254 1.71E-50 YLR170C APS1 hom Small subunit of the clathrin-associated adaptor complex AP-1, which is involved in protein sorting at the trans-Golgi network; homolog of the sigma subunit of the mammalian clathrin AP-1 complex
0.248 2.37E-48 YHL019C APM2 hom Protein of unknown function, homologous to the medium chain of mammalian clathrin-associated protein complex; involved in vesicular transport
0.240 4.36E-45 YER180C-A SLO1 hom Protein interacting with Arl3p, which is a GTPase of the Ras superfamily involved in vesicle-tethering at the Golgi; putative ortholog of human SCOCO
0.228 1.40E-40 YLR171W_d YLR171W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.206 2.31E-33 YLR169W_d YLR169W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.202 2.62E-32 YMR071C TVP18 hom Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments
0.189 2.03E-28 YJL085W EXO70 het Subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis prior to SNARE-mediated fusion; PtdIns[4,5]P2-binding protein that localizes to exocytic sites in an actin-independent manner, targeting and anchoring the exocyst with Sec3p; involved in exocyst assembly; direct downstream effector of Rho3p and Cdc42p; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.178 2.63E-25 YLR083C EMP70 hom Protein with a role in cellular adhesion, filamentous growth, and endosome-to-vacuole sorting; similar to Tmn2p and Tmn3p; member of Transmembrane Nine family of proteins with 9 transmembrane segments