YDR170C / SEC7

Guanine nucleotide exchange factor (GEF) for ADP ribosylation factors involved in proliferation of the Golgi, intra-Golgi transport and ER-to-Golgi transport; found in the cytoplasm and on Golgi-associated coated vesicles

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR170C / SEC7

Click on Significant Values for Screen Details ID:SGTC_40|Compound:0327-0103|FD-Score:-3.49|P-value:2.09E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:3.26|P-value:5.19E-4 ID:SGTC_112|Compound:phenothiazine|FD-Score:3.18|P-value:7.02E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-4.32|P-value:6.24E-6 ID:SGTC_168|Compound:3970-0862|FD-Score:-4.31|P-value:6.54E-6 ID:SGTC_514|Compound:4466-0038|FD-Score:4.50|P-value:2.91E-6 ID:SGTC_540|Compound:1187-0821|FD-Score:3.15|P-value:7.66E-4 ID:SGTC_637|Compound:1181-0519|FD-Score:-3.39|P-value:3.01E-4 ID:SGTC_740|Compound:k014-0057|FD-Score:3.26|P-value:5.25E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:-3.91|P-value:3.78E-5 ID:SGTC_780|Compound:0109-0199|FD-Score:4.31|P-value:7.20E-6 ID:SGTC_874|Compound:0599-0792|FD-Score:3.29|P-value:4.77E-4 ID:SGTC_999|Compound:4022-0066|FD-Score:47.40|P-value:0 ID:SGTC_1003|Compound:4049-0218|FD-Score:-3.45|P-value:2.38E-4 ID:SGTC_1097|Compound:itavastatin|FD-Score:-3.10|P-value:8.63E-4 ID:SGTC_1098|Compound:clofazimine|FD-Score:3.24|P-value:5.69E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:3.59|P-value:1.54E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:-3.13|P-value:7.63E-4 ID:SGTC_1531|Compound:st059297|FD-Score:3.37|P-value:3.52E-4 ID:SGTC_1556|Compound:plumbagin|FD-Score:3.56|P-value:1.70E-4 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:3.80|P-value:6.73E-5 ID:SGTC_1754|Compound:st042065|FD-Score:3.34|P-value:3.86E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:4.12|P-value:1.71E-5 ID:SGTC_1800|Compound:st050079|FD-Score:4.34|P-value:6.16E-6 ID:SGTC_1803|Compound:st050068|FD-Score:3.12|P-value:8.51E-4 ID:SGTC_1827|Compound:berberine|FD-Score:3.32|P-value:4.16E-4 ID:SGTC_1838|Compound:st055369|FD-Score:4.28|P-value:8.11E-6 ID:SGTC_2003|Compound:4023581|FD-Score:-3.69|P-value:9.56E-5 ID:SGTC_2076|Compound:5212012|FD-Score:-3.26|P-value:4.91E-4 ID:SGTC_2217|Compound:6934799|FD-Score:-3.18|P-value:6.54E-4 ID:SGTC_2242|Compound:6630449|FD-Score:40.20|P-value:0 ID:SGTC_2321|Compound:7390090|FD-Score:4.22|P-value:1.09E-5 ID:SGTC_2336|Compound:9001819|FD-Score:3.72|P-value:9.03E-5 ID:SGTC_2428|Compound:5615643|FD-Score:3.76|P-value:7.79E-5 ID:SGTC_2479|Compound:5617353|FD-Score:-4.17|P-value:1.26E-5 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:4.68|P-value:1.22E-6 ID:SGTC_2642|Compound:cholecalciferol|FD-Score:-4.47|P-value:3.02E-6 ID:SGTC_2657|Compound:nystatin|FD-Score:4.70|P-value:1.14E-6 ID:SGTC_2896|Compound:9052656|FD-Score:-3.82|P-value:5.48E-5 ID:SGTC_2965|Compound:9082772|FD-Score:-5.00|P-value:2.18E-7 ID:SGTC_2985|Compound:9022375|FD-Score:3.12|P-value:8.44E-4 ID:SGTC_3000|Compound:9075062|FD-Score:-9.80|P-value:2.26E-23 ID:SGTC_3004|Compound:9076122|FD-Score:7.54|P-value:1.56E-14 ID:SGTC_3013|Compound:9080133|FD-Score:3.54|P-value:1.88E-4 ID:SGTC_3014|Compound:9081533|FD-Score:-5.44|P-value:1.97E-8 ID:SGTC_3015|Compound:9080845|FD-Score:-3.11|P-value:8.12E-4 ID:SGTC_3018|Compound:9082602|FD-Score:-3.53|P-value:1.75E-4 ID:SGTC_3019|Compound:9081971|FD-Score:-6.45|P-value:3.50E-11 ID:SGTC_3037|Compound:9091026|FD-Score:5.22|P-value:7.51E-8 ID:SGTC_3080|Compound:9117754|FD-Score:-3.10|P-value:8.54E-4 ID:SGTC_3115|Compound:9123671|FD-Score:3.42|P-value:2.89E-4 ID:SGTC_3151|Compound:9099821|FD-Score:-3.33|P-value:3.71E-4 ID:SGTC_3159|Compound:9100096|FD-Score:4.21|P-value:1.16E-5 ID:SGTC_3293|Compound:9120939|FD-Score:-5.05|P-value:1.66E-7 ID:SGTC_40|Compound:0327-0103|FD-Score:-3.49|P-value:2.09E-4 ID:SGTC_70|Compound:0141-0289|FD-Score:3.26|P-value:5.19E-4 ID:SGTC_112|Compound:phenothiazine|FD-Score:3.18|P-value:7.02E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:-4.32|P-value:6.24E-6 ID:SGTC_168|Compound:3970-0862|FD-Score:-4.31|P-value:6.54E-6 ID:SGTC_514|Compound:4466-0038|FD-Score:4.50|P-value:2.91E-6 ID:SGTC_540|Compound:1187-0821|FD-Score:3.15|P-value:7.66E-4 ID:SGTC_637|Compound:1181-0519|FD-Score:-3.39|P-value:3.01E-4 ID:SGTC_740|Compound:k014-0057|FD-Score:3.26|P-value:5.25E-4 ID:SGTC_768|Compound:0384-0033|FD-Score:-3.91|P-value:3.78E-5 ID:SGTC_780|Compound:0109-0199|FD-Score:4.31|P-value:7.20E-6 ID:SGTC_874|Compound:0599-0792|FD-Score:3.29|P-value:4.77E-4 ID:SGTC_999|Compound:4022-0066|FD-Score:47.40|P-value:0 ID:SGTC_1003|Compound:4049-0218|FD-Score:-3.45|P-value:2.38E-4 ID:SGTC_1097|Compound:itavastatin|FD-Score:-3.10|P-value:8.63E-4 ID:SGTC_1098|Compound:clofazimine|FD-Score:3.24|P-value:5.69E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:3.59|P-value:1.54E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:-3.13|P-value:7.63E-4 ID:SGTC_1531|Compound:st059297|FD-Score:3.37|P-value:3.52E-4 ID:SGTC_1556|Compound:plumbagin|FD-Score:3.56|P-value:1.70E-4 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:3.80|P-value:6.73E-5 ID:SGTC_1754|Compound:st042065|FD-Score:3.34|P-value:3.86E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:4.12|P-value:1.71E-5 ID:SGTC_1800|Compound:st050079|FD-Score:4.34|P-value:6.16E-6 ID:SGTC_1803|Compound:st050068|FD-Score:3.12|P-value:8.51E-4 ID:SGTC_1827|Compound:berberine|FD-Score:3.32|P-value:4.16E-4 ID:SGTC_1838|Compound:st055369|FD-Score:4.28|P-value:8.11E-6 ID:SGTC_2003|Compound:4023581|FD-Score:-3.69|P-value:9.56E-5 ID:SGTC_2076|Compound:5212012|FD-Score:-3.26|P-value:4.91E-4 ID:SGTC_2217|Compound:6934799|FD-Score:-3.18|P-value:6.54E-4 ID:SGTC_2242|Compound:6630449|FD-Score:40.20|P-value:0 ID:SGTC_2321|Compound:7390090|FD-Score:4.22|P-value:1.09E-5 ID:SGTC_2336|Compound:9001819|FD-Score:3.72|P-value:9.03E-5 ID:SGTC_2428|Compound:5615643|FD-Score:3.76|P-value:7.79E-5 ID:SGTC_2479|Compound:5617353|FD-Score:-4.17|P-value:1.26E-5 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:4.68|P-value:1.22E-6 ID:SGTC_2642|Compound:cholecalciferol|FD-Score:-4.47|P-value:3.02E-6 ID:SGTC_2657|Compound:nystatin|FD-Score:4.70|P-value:1.14E-6 ID:SGTC_2896|Compound:9052656|FD-Score:-3.82|P-value:5.48E-5 ID:SGTC_2965|Compound:9082772|FD-Score:-5.00|P-value:2.18E-7 ID:SGTC_2985|Compound:9022375|FD-Score:3.12|P-value:8.44E-4 ID:SGTC_3000|Compound:9075062|FD-Score:-9.80|P-value:2.26E-23 ID:SGTC_3004|Compound:9076122|FD-Score:7.54|P-value:1.56E-14 ID:SGTC_3013|Compound:9080133|FD-Score:3.54|P-value:1.88E-4 ID:SGTC_3014|Compound:9081533|FD-Score:-5.44|P-value:1.97E-8 ID:SGTC_3015|Compound:9080845|FD-Score:-3.11|P-value:8.12E-4 ID:SGTC_3018|Compound:9082602|FD-Score:-3.53|P-value:1.75E-4 ID:SGTC_3019|Compound:9081971|FD-Score:-6.45|P-value:3.50E-11 ID:SGTC_3037|Compound:9091026|FD-Score:5.22|P-value:7.51E-8 ID:SGTC_3080|Compound:9117754|FD-Score:-3.10|P-value:8.54E-4 ID:SGTC_3115|Compound:9123671|FD-Score:3.42|P-value:2.89E-4 ID:SGTC_3151|Compound:9099821|FD-Score:-3.33|P-value:3.71E-4 ID:SGTC_3159|Compound:9100096|FD-Score:4.21|P-value:1.16E-5 ID:SGTC_3293|Compound:9120939|FD-Score:-5.05|P-value:1.66E-7

Top fitness defect scores for YDR170C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_999 4022-0066 47.40 22.7 µM 0 Golgi 1 0 39.50
2 SGTC_2242 6630449 40.20 61.3 µM 0 Golgi 1 0 32.60
3 SGTC_3004 9076122 7.54 71.4 µM 1.56E-14 1 2.39E-14 1.97
4 SGTC_3037 9091026 5.22 49.5 µM 7.51E-8 NEO1-PIK1 16 9.05E-8 0.44
5 SGTC_2657 nystatin 4.70 74.4 µM 1.14E-6 1 1.33E-6 0.38
6 SGTC_2541 muurolladie-3-one 4.68 83.1 µM 1.22E-6 excess fatty acid 15 1.41E-6 0.33
7 SGTC_514 4466-0038 4.50 2.6 nM 2.91E-6 cell wall 32 3.33E-6 0.17
8 SGTC_1800 st050079 4.34 29.1 µM 6.16E-6 NEO1-PIK1 20 6.96E-6 0.01
9 SGTC_780 0109-0199 4.31 192.0 µM 7.20E-6 3 8.12E-6 0.48
10 SGTC_1838 st055369 4.28 84.5 µM 8.11E-6 4 9.14E-6 0.14
11 SGTC_2321 7390090 4.22 138.8 µM 1.09E-5 3 1.23E-5 0.08
12 SGTC_3159 9100096 4.21 49.5 µM 1.16E-5 4 1.30E-5 0.26
13 SGTC_1785 bathophenanthroline 4.12 24.0 µM 1.71E-5 heme requiring 21 1.91E-5 0.06
14 SGTC_1595 6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin 3.80 67.5 µM 6.73E-5 10 7.36E-5 0.02
15 SGTC_2428 5615643 3.76 7.8 µM 7.79E-5 7 8.50E-5 0.29
16 SGTC_2336 9001819 3.72 198.7 µM 9.03E-5 heme biosynthesis & mitochondrial translocase 42 9.83E-5 0.09
17 SGTC_1203 3992-3522 3.59 103.0 µM 1.54E-4 18 1.67E-4 0.10
18 SGTC_1556 plumbagin 3.56 1.5 µM 1.70E-4 superoxide 24 1.83E-4 0.03
19 SGTC_3013 9080133 3.54 71.4 µM 1.88E-4 4 2.02E-4 0.07
20 SGTC_3115 9123671 3.42 49.5 µM 2.89E-4 4 3.10E-4 0.23

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.232 5.25E-42 YJL124C LSM1 hom Lsm (Like Sm) protein; forms heteroheptameric complex (with Lsm2p, Lsm3p, Lsm4p, Lsm5p, Lsm6p, and Lsm7p) involved in degradation of cytoplasmic mRNAs; unlike most Sm-like proteins, Lsm1p requires both its SM-domain and C-terminal domain for RNA-binding; forms cytoplasmic foci upon DNA replication stress
0.229 4.53E-41 YBR164C ARL1 hom Soluble GTPase with a role in regulation of membrane traffic; regulates potassium influx; G protein of the Ras superfamily, similar to ADP-ribosylation factor
0.224 2.15E-39 YJL004C SYS1 hom Integral membrane protein of the Golgi required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
0.206 1.23E-33 YNR051C BRE5 hom Ubiquitin protease cofactor, forms deubiquitination complex with Ubp3p that coregulates anterograde and retrograde transport between the endoplasmic reticulum and Golgi compartments; null is sensitive to brefeldin A
0.179 1.48E-25 YDR389W SAC7 hom GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate
0.160 9.73E-21 YPL051W ARL3 hom GTPase of the Ras superfamily required to recruit Arl1p to the Golgi; similar to ADP-ribosylation factor
0.153 5.54E-19 YEL022W GEA2 hom Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication
0.150 2.81E-18 YDL031W DBP10 het Putative ATP-dependent RNA helicase of the DEAD-box protein family, constituent of 66S pre-ribosomal particles; essential protein involved in ribosome biogenesis
0.144 5.27E-17 YCR006C_d YCR006C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.140 3.58E-16 YNL147W LSM7 hom Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; protein abundance increases and forms cytoplasmic foci in response to DNA replication stress
0.134 7.64E-15 YKR001C VPS1 hom Dynamin-like GTPase required for vacuolar sorting; also involved in actin cytoskeleton organization, endocytosis, late Golgi-retention of some proteins, regulation of peroxisome biogenesis
0.130 4.95E-14 YNL053W MSG5 hom Dual-specificity protein phosphatase; exists in 2 isoforms; required for maintenance of a low level of signaling through the cell integrity pathway, adaptive response to pheromone; regulates and is regulated by Slt2p; dephosphorylates Fus3p
0.127 1.41E-13 YCR021C HSP30 hom Hydrophobic plasma membrane localized, stress-responsive protein that negatively regulates the H(+)-ATPase Pma1p; induced by heat shock, ethanol treatment, weak organic acid, glucose limitation, and entry into stationary phase
0.127 1.81E-13 YKR040C_d YKR040C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YKR041W
0.121 2.30E-12 YDR162C NBP2 hom Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM)