YDR177W / UBC1

Ubiquitin-conjugating enzyme; mediates selective degradation of short-lived and abnormal proteins; plays a role in vesicle biogenesis and ER-associated protein degradation (ERAD); component of the cellular stress response; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR177W / UBC1

Click on Significant Values for Screen Details ID:SGTC_195|Compound:4234-0266|FD-Score:3.59|P-value:5.93E-4 ID:SGTC_223|Compound:busulfan|FD-Score:3.46|P-value:8.84E-4 ID:SGTC_276|Compound:colchicine|FD-Score:-3.88|P-value:2.04E-4 ID:SGTC_600|Compound:1495-0418|FD-Score:-3.82|P-value:2.53E-4 ID:SGTC_605|Compound:1315-0399|FD-Score:-3.97|P-value:1.52E-4 ID:SGTC_612|Compound:0302-0167|FD-Score:-4.39|P-value:3.22E-5 ID:SGTC_623|Compound:k072-0108|FD-Score:-4.14|P-value:8.24E-5 ID:SGTC_635|Compound:k038-0028|FD-Score:4.14|P-value:9.30E-5 ID:SGTC_691|Compound:1548-0686|FD-Score:3.45|P-value:9.23E-4 ID:SGTC_848|Compound:0368-0089|FD-Score:4.16|P-value:8.41E-5 ID:SGTC_930|Compound:2810-3379|FD-Score:3.63|P-value:5.26E-4 ID:SGTC_940|Compound:3232-1287|FD-Score:-4.70|P-value:9.62E-6 ID:SGTC_1065|Compound:prochlorperazine maleate|FD-Score:6.03|P-value:2.45E-8 ID:SGTC_1112|Compound:tnp00069|FD-Score:3.83|P-value:2.75E-4 ID:SGTC_1145|Compound:3546-0597|FD-Score:3.47|P-value:8.64E-4 ID:SGTC_1151|Compound:3909-8316|FD-Score:5.90|P-value:4.67E-8 ID:SGTC_1206|Compound:3978-0017|FD-Score:3.69|P-value:4.36E-4 ID:SGTC_1272|Compound:0839-0021|FD-Score:4.13|P-value:9.53E-5 ID:SGTC_1282|Compound:0886-0010|FD-Score:5.21|P-value:1.23E-6 ID:SGTC_1321|Compound:1326-2144|FD-Score:4.24|P-value:6.41E-5 ID:SGTC_1328|Compound:1441-0025|FD-Score:4.42|P-value:3.30E-5 ID:SGTC_1334|Compound:1486-0610|FD-Score:3.67|P-value:4.67E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:-6.60|P-value:9.77E-10 ID:SGTC_1366|Compound:1683-0113|FD-Score:3.58|P-value:6.07E-4 ID:SGTC_1376|Compound:2910-0861|FD-Score:3.77|P-value:3.34E-4 ID:SGTC_1477|Compound:0139-0234|FD-Score:4.43|P-value:3.17E-5 ID:SGTC_1880|Compound:MG-132|FD-Score:5.52|P-value:3.00E-7 ID:SGTC_1933|Compound:st074711|FD-Score:4.65|P-value:1.33E-5 ID:SGTC_1964|Compound:st076914|FD-Score:4.89|P-value:4.95E-6 ID:SGTC_2015|Compound:5119600|FD-Score:-4.66|P-value:1.11E-5 ID:SGTC_2021|Compound:4019055|FD-Score:3.48|P-value:8.43E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-3.92|P-value:1.80E-4 ID:SGTC_2124|Compound:5246320|FD-Score:-3.51|P-value:6.96E-4 ID:SGTC_2267|Compound:7931562|FD-Score:3.52|P-value:7.51E-4 ID:SGTC_2292|Compound:7671323|FD-Score:3.47|P-value:8.57E-4 ID:SGTC_2328|Compound:6201408|FD-Score:3.89|P-value:2.18E-4 ID:SGTC_2350|Compound:9023895|FD-Score:3.93|P-value:1.92E-4 ID:SGTC_2391|Compound:6285546|FD-Score:3.65|P-value:4.95E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:5.68|P-value:1.37E-7 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:3.95|P-value:1.83E-4 ID:SGTC_2569|Compound:tetrandrine|FD-Score:3.53|P-value:7.12E-4 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:4.85|P-value:5.88E-6 ID:SGTC_2739|Compound:tacrine|FD-Score:-3.47|P-value:7.92E-4 ID:SGTC_2791|Compound:5663188|FD-Score:3.49|P-value:8.05E-4 ID:SGTC_2794|Compound:7785382|FD-Score:4.95|P-value:3.85E-6 ID:SGTC_2832|Compound:7992762|FD-Score:3.63|P-value:5.32E-4 ID:SGTC_2846|Compound:9008642|FD-Score:8.06|P-value:1.49E-13 ID:SGTC_2850|Compound:9015429|FD-Score:-4.93|P-value:3.68E-6 ID:SGTC_2871|Compound:9038510|FD-Score:-4.81|P-value:5.92E-6 ID:SGTC_2885|Compound:9057732|FD-Score:3.71|P-value:3.98E-4 ID:SGTC_2890|Compound:9046729|FD-Score:-4.40|P-value:3.05E-5 ID:SGTC_2893|Compound:9048488|FD-Score:-6.24|P-value:6.85E-9 ID:SGTC_2895|Compound:9054447|FD-Score:-3.43|P-value:8.94E-4 ID:SGTC_2987|Compound:9018338|FD-Score:4.71|P-value:1.03E-5 ID:SGTC_3062|Compound:9077494|FD-Score:-7.21|P-value:2.89E-11 ID:SGTC_3072|Compound:9116419|FD-Score:3.73|P-value:3.74E-4 ID:SGTC_3139|Compound:9095020|FD-Score:4.02|P-value:1.39E-4 ID:SGTC_3162|Compound:9099653|FD-Score:-3.78|P-value:2.88E-4 ID:SGTC_3164|Compound:9100705|FD-Score:-3.54|P-value:6.32E-4 ID:SGTC_3175|Compound:9106517|FD-Score:3.50|P-value:7.98E-4 ID:SGTC_195|Compound:4234-0266|FD-Score:3.59|P-value:5.93E-4 ID:SGTC_223|Compound:busulfan|FD-Score:3.46|P-value:8.84E-4 ID:SGTC_276|Compound:colchicine|FD-Score:-3.88|P-value:2.04E-4 ID:SGTC_600|Compound:1495-0418|FD-Score:-3.82|P-value:2.53E-4 ID:SGTC_605|Compound:1315-0399|FD-Score:-3.97|P-value:1.52E-4 ID:SGTC_612|Compound:0302-0167|FD-Score:-4.39|P-value:3.22E-5 ID:SGTC_623|Compound:k072-0108|FD-Score:-4.14|P-value:8.24E-5 ID:SGTC_635|Compound:k038-0028|FD-Score:4.14|P-value:9.30E-5 ID:SGTC_691|Compound:1548-0686|FD-Score:3.45|P-value:9.23E-4 ID:SGTC_848|Compound:0368-0089|FD-Score:4.16|P-value:8.41E-5 ID:SGTC_930|Compound:2810-3379|FD-Score:3.63|P-value:5.26E-4 ID:SGTC_940|Compound:3232-1287|FD-Score:-4.70|P-value:9.62E-6 ID:SGTC_1065|Compound:prochlorperazine maleate|FD-Score:6.03|P-value:2.45E-8 ID:SGTC_1112|Compound:tnp00069|FD-Score:3.83|P-value:2.75E-4 ID:SGTC_1145|Compound:3546-0597|FD-Score:3.47|P-value:8.64E-4 ID:SGTC_1151|Compound:3909-8316|FD-Score:5.90|P-value:4.67E-8 ID:SGTC_1206|Compound:3978-0017|FD-Score:3.69|P-value:4.36E-4 ID:SGTC_1272|Compound:0839-0021|FD-Score:4.13|P-value:9.53E-5 ID:SGTC_1282|Compound:0886-0010|FD-Score:5.21|P-value:1.23E-6 ID:SGTC_1321|Compound:1326-2144|FD-Score:4.24|P-value:6.41E-5 ID:SGTC_1328|Compound:1441-0025|FD-Score:4.42|P-value:3.30E-5 ID:SGTC_1334|Compound:1486-0610|FD-Score:3.67|P-value:4.67E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:-6.60|P-value:9.77E-10 ID:SGTC_1366|Compound:1683-0113|FD-Score:3.58|P-value:6.07E-4 ID:SGTC_1376|Compound:2910-0861|FD-Score:3.77|P-value:3.34E-4 ID:SGTC_1477|Compound:0139-0234|FD-Score:4.43|P-value:3.17E-5 ID:SGTC_1880|Compound:MG-132|FD-Score:5.52|P-value:3.00E-7 ID:SGTC_1933|Compound:st074711|FD-Score:4.65|P-value:1.33E-5 ID:SGTC_1964|Compound:st076914|FD-Score:4.89|P-value:4.95E-6 ID:SGTC_2015|Compound:5119600|FD-Score:-4.66|P-value:1.11E-5 ID:SGTC_2021|Compound:4019055|FD-Score:3.48|P-value:8.43E-4 ID:SGTC_2054|Compound:5233173|FD-Score:-3.92|P-value:1.80E-4 ID:SGTC_2124|Compound:5246320|FD-Score:-3.51|P-value:6.96E-4 ID:SGTC_2267|Compound:7931562|FD-Score:3.52|P-value:7.51E-4 ID:SGTC_2292|Compound:7671323|FD-Score:3.47|P-value:8.57E-4 ID:SGTC_2328|Compound:6201408|FD-Score:3.89|P-value:2.18E-4 ID:SGTC_2350|Compound:9023895|FD-Score:3.93|P-value:1.92E-4 ID:SGTC_2391|Compound:6285546|FD-Score:3.65|P-value:4.95E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:5.68|P-value:1.37E-7 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:3.95|P-value:1.83E-4 ID:SGTC_2569|Compound:tetrandrine|FD-Score:3.53|P-value:7.12E-4 ID:SGTC_2636|Compound:apigenin dimethyl ether|FD-Score:4.85|P-value:5.88E-6 ID:SGTC_2739|Compound:tacrine|FD-Score:-3.47|P-value:7.92E-4 ID:SGTC_2791|Compound:5663188|FD-Score:3.49|P-value:8.05E-4 ID:SGTC_2794|Compound:7785382|FD-Score:4.95|P-value:3.85E-6 ID:SGTC_2832|Compound:7992762|FD-Score:3.63|P-value:5.32E-4 ID:SGTC_2846|Compound:9008642|FD-Score:8.06|P-value:1.49E-13 ID:SGTC_2850|Compound:9015429|FD-Score:-4.93|P-value:3.68E-6 ID:SGTC_2871|Compound:9038510|FD-Score:-4.81|P-value:5.92E-6 ID:SGTC_2885|Compound:9057732|FD-Score:3.71|P-value:3.98E-4 ID:SGTC_2890|Compound:9046729|FD-Score:-4.40|P-value:3.05E-5 ID:SGTC_2893|Compound:9048488|FD-Score:-6.24|P-value:6.85E-9 ID:SGTC_2895|Compound:9054447|FD-Score:-3.43|P-value:8.94E-4 ID:SGTC_2987|Compound:9018338|FD-Score:4.71|P-value:1.03E-5 ID:SGTC_3062|Compound:9077494|FD-Score:-7.21|P-value:2.89E-11 ID:SGTC_3072|Compound:9116419|FD-Score:3.73|P-value:3.74E-4 ID:SGTC_3139|Compound:9095020|FD-Score:4.02|P-value:1.39E-4 ID:SGTC_3162|Compound:9099653|FD-Score:-3.78|P-value:2.88E-4 ID:SGTC_3164|Compound:9100705|FD-Score:-3.54|P-value:6.32E-4 ID:SGTC_3175|Compound:9106517|FD-Score:3.50|P-value:7.98E-4

Top fitness defect scores for YDR177W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2846 9008642 8.06 19.5 µM 1.49E-13 1 3.79E-16 3.12
2 SGTC_1065 prochlorperazine maleate 6.03 2.5 µM 2.45E-8 1 8.16E-10 0.66
3 SGTC_1151 3909-8316 5.90 15.3 µM 4.67E-8 1 1.78E-9 0.60
4 SGTC_2502 avocadyne acetate 5.68 18.6 µM 1.37E-7 2 6.55E-9 0.86
5 SGTC_1880 MG-132 5.52 525.0 µM 3.00E-7 11 1.70E-8 0.08
6 SGTC_1282 0886-0010 5.21 32.4 µM 1.23E-6 endomembrane recycling 6 9.41E-8 0.13
7 SGTC_2794 7785382 4.95 27.3 µM 3.85E-6 9 3.74E-7 0.62
8 SGTC_1964 st076914 4.89 61.0 µM 4.95E-6 RPP1 & pyrimidine depletion 4 5.06E-7 0.59
9 SGTC_2636 apigenin dimethyl ether 4.85 100.0 µM 5.88E-6 4 6.23E-7 0.08
10 SGTC_2987 9018338 4.71 71.4 µM 1.03E-5 RPP1 & pyrimidine depletion 5 1.23E-6 0.51
11 SGTC_1933 st074711 4.65 15.9 µM 1.33E-5 5 1.68E-6 0.42
12 SGTC_1477 0139-0234 4.43 16.0 µM 3.17E-5 2 4.79E-6 0.53
13 SGTC_1328 1441-0025 4.42 69.2 µM 3.30E-5 3 5.03E-6 0.73
14 SGTC_1321 1326-2144 4.24 215.0 µM 6.41E-5 3 1.12E-5 0.42
15 SGTC_848 0368-0089 4.16 85.6 µM 8.41E-5 iron homeostasis 9 1.56E-5 0.25
16 SGTC_635 k038-0028 4.14 24.7 µM 9.30E-5 ubiquinone biosynthesis & proteasome 15 1.76E-5 0.13
17 SGTC_1272 0839-0021 4.13 27.0 µM 9.53E-5 4 1.81E-5 0.01
18 SGTC_3139 9095020 4.02 49.5 µM 1.39E-4 4 2.85E-5 0.07
19 SGTC_2565 isoliquiritigenin 3.95 100.0 µM 1.83E-4 11 3.98E-5 0.05
20 SGTC_2350 9023895 3.93 38.9 µM 1.92E-4 ergosterol biosynthesis 8 4.22E-5 0.09

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.104 1.59E-9 YBR193C MED8 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
0.103 2.03E-9 YKL042W SPC42 het Central plaque component of spindle pole body (SPB); involved in SPB duplication, may facilitate attachment of the SPB to the nuclear membrane
0.095 3.54E-8 YDR373W FRQ1 het N-myristoylated calcium-binding protein that may have a role in intracellular signaling through its regulation of the phosphatidylinositol 4-kinase Pik1p; member of the recoverin/frequenin branch of the EF-hand superfamily
0.092 9.31E-8 YER038C KRE29 het Subunit of the SMC5-SMC6 complex; this complex is involved in removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; heterozygous mutant shows haploinsufficiency in K1 killer toxin resistance
0.090 1.54E-7 YNL161W CBK1 het Serine/threonine protein kinase of the the RAM signaling network; Ndr/LATS family member; binds regulatory subunit Mob2p; involved in regulation of cellular morphogenesis, polarized growth, and septum destruction; phosphorylation by Cbk1p regulates localization and activity of Ace2p transcription factor and Ssd1p translational repressor; Cbk1p activity is regulated by both phosphorylation and specific localization; relocalizes to cytoplasm upon DNA replication stress
0.089 2.39E-7 YKL153W_d YKL153W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant
0.087 4.03E-7 YDR434W GPI17 het Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog
0.086 6.21E-7 YOR103C OST2 het Epsilon subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins
0.086 6.27E-7 YML046W PRP39 het U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats
0.086 6.92E-7 YMR218C TRS130 het One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in ER to Golgi membrane traffic; mutation activates transcription of OCH1; mutants are defective in cytoplasm-to-vacuole targeting (Cvt) pathway and starvation-induced autophagy
0.085 7.09E-7 YIL147C SLN1 het Histidine kinase osmosensor that regulates a MAP kinase cascade; transmembrane protein with an intracellular kinase domain that signals to Ypd1p and Ssk1p, thereby forming a phosphorelay system similar to bacterial two-component regulators
0.085 8.46E-7 YBL023C MCM2 het Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex; relative distribution to the nucleus increases upon DNA replication stress
0.083 1.47E-6 YER094C PUP3 het Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10
0.083 1.62E-6 YGL011C SCL1 het Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
0.083 1.66E-6 YDR086C SSS1 het Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p