YDR195W / REF2

RNA-binding protein involved in the cleavage step of mRNA 3'-end formation prior to polyadenylation, and in snoRNA maturation; part of holo-CPF subcomplex APT, which associates with 3'-ends of snoRNA- and mRNA-encoding genes

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR195W / REF2

Click on Significant Values for Screen Details ID:SGTC_41|Compound:0478-0632|FD-Score:-3.37|P-value:6.84E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:3.83|P-value:1.87E-4 ID:SGTC_57|Compound:miconazole|FD-Score:-3.45|P-value:5.28E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:-3.40|P-value:6.20E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:4.23|P-value:4.20E-5 ID:SGTC_229|Compound:benomyl|FD-Score:3.74|P-value:2.51E-4 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:4.04|P-value:8.72E-5 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:3.61|P-value:4.03E-4 ID:SGTC_253|Compound:dyclonine|FD-Score:3.54|P-value:4.97E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.57|P-value:7.69E-6 ID:SGTC_467|Compound:ro 31-8220|FD-Score:3.53|P-value:5.20E-4 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:3.59|P-value:4.30E-4 ID:SGTC_526|Compound:4469-0568|FD-Score:-3.88|P-value:1.18E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:3.59|P-value:4.33E-4 ID:SGTC_562|Compound:3772-2733|FD-Score:3.41|P-value:7.63E-4 ID:SGTC_569|Compound:4130-2812|FD-Score:-4.14|P-value:4.33E-5 ID:SGTC_666|Compound:0272-0154|FD-Score:3.39|P-value:8.20E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.62|P-value:8.38E-6 ID:SGTC_691|Compound:1548-0686|FD-Score:4.91|P-value:2.35E-6 ID:SGTC_800|Compound:3448-7362|FD-Score:4.02|P-value:9.35E-5 ID:SGTC_942|Compound:3253-1115|FD-Score:-3.27|P-value:9.64E-4 ID:SGTC_1144|Compound:3486-0183|FD-Score:-18.10|P-value:3.98E-65 ID:SGTC_1166|Compound:k206-0078|FD-Score:-3.36|P-value:7.18E-4 ID:SGTC_1214|Compound:0358-0031|FD-Score:-4.03|P-value:6.66E-5 ID:SGTC_1323|Compound:1348-1351|FD-Score:-3.35|P-value:7.36E-4 ID:SGTC_1400|Compound:3771-8998|FD-Score:-3.32|P-value:8.01E-4 ID:SGTC_1687|Compound:st020778|FD-Score:7.01|P-value:3.00E-11 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.05|P-value:6.22E-5 ID:SGTC_1853|Compound:st056710|FD-Score:-4.08|P-value:5.49E-5 ID:SGTC_1875|Compound:nocodazole|FD-Score:5.66|P-value:6.58E-8 ID:SGTC_1877|Compound:wortmannin|FD-Score:7.21|P-value:8.03E-12 ID:SGTC_2077|Compound:5212015|FD-Score:4.65|P-value:7.47E-6 ID:SGTC_2090|Compound:5301565|FD-Score:4.21|P-value:4.51E-5 ID:SGTC_2108|Compound:5365811|FD-Score:-4.04|P-value:6.48E-5 ID:SGTC_2141|Compound:5621638|FD-Score:5.13|P-value:8.76E-7 ID:SGTC_2161|Compound:5662504|FD-Score:3.66|P-value:3.39E-4 ID:SGTC_2200|Compound:6600604|FD-Score:4.06|P-value:7.96E-5 ID:SGTC_2202|Compound:7226349|FD-Score:3.75|P-value:2.46E-4 ID:SGTC_2322|Compound:7413319|FD-Score:5.10|P-value:1.02E-6 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:5.57|P-value:1.02E-7 ID:SGTC_2826|Compound:7992541|FD-Score:3.39|P-value:8.21E-4 ID:SGTC_2909|Compound:7941780|FD-Score:3.91|P-value:1.37E-4 ID:SGTC_2913|Compound:7966556|FD-Score:-4.90|P-value:1.80E-6 ID:SGTC_2916|Compound:7875993|FD-Score:-7.10|P-value:1.08E-11 ID:SGTC_2989|Compound:9073649|FD-Score:3.96|P-value:1.15E-4 ID:SGTC_3018|Compound:9082602|FD-Score:-5.24|P-value:3.59E-7 ID:SGTC_3136|Compound:9095672|FD-Score:3.42|P-value:7.48E-4 ID:SGTC_3152|Compound:9099253|FD-Score:-4.28|P-value:2.52E-5 ID:SGTC_3201|Compound:9113999|FD-Score:5.85|P-value:2.48E-8 ID:SGTC_41|Compound:0478-0632|FD-Score:-3.37|P-value:6.84E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:3.83|P-value:1.87E-4 ID:SGTC_57|Compound:miconazole|FD-Score:-3.45|P-value:5.28E-4 ID:SGTC_116|Compound:0155-0182|FD-Score:-3.40|P-value:6.20E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:4.23|P-value:4.20E-5 ID:SGTC_229|Compound:benomyl|FD-Score:3.74|P-value:2.51E-4 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:4.04|P-value:8.72E-5 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:3.61|P-value:4.03E-4 ID:SGTC_253|Compound:dyclonine|FD-Score:3.54|P-value:4.97E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-4.57|P-value:7.69E-6 ID:SGTC_467|Compound:ro 31-8220|FD-Score:3.53|P-value:5.20E-4 ID:SGTC_475|Compound:α-linolenic acid|FD-Score:3.59|P-value:4.30E-4 ID:SGTC_526|Compound:4469-0568|FD-Score:-3.88|P-value:1.18E-4 ID:SGTC_528|Compound:3492-0019|FD-Score:3.59|P-value:4.33E-4 ID:SGTC_562|Compound:3772-2733|FD-Score:3.41|P-value:7.63E-4 ID:SGTC_569|Compound:4130-2812|FD-Score:-4.14|P-value:4.33E-5 ID:SGTC_666|Compound:0272-0154|FD-Score:3.39|P-value:8.20E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.62|P-value:8.38E-6 ID:SGTC_691|Compound:1548-0686|FD-Score:4.91|P-value:2.35E-6 ID:SGTC_800|Compound:3448-7362|FD-Score:4.02|P-value:9.35E-5 ID:SGTC_942|Compound:3253-1115|FD-Score:-3.27|P-value:9.64E-4 ID:SGTC_1144|Compound:3486-0183|FD-Score:-18.10|P-value:3.98E-65 ID:SGTC_1166|Compound:k206-0078|FD-Score:-3.36|P-value:7.18E-4 ID:SGTC_1214|Compound:0358-0031|FD-Score:-4.03|P-value:6.66E-5 ID:SGTC_1323|Compound:1348-1351|FD-Score:-3.35|P-value:7.36E-4 ID:SGTC_1400|Compound:3771-8998|FD-Score:-3.32|P-value:8.01E-4 ID:SGTC_1687|Compound:st020778|FD-Score:7.01|P-value:3.00E-11 ID:SGTC_1718|Compound:roemerine|FD-Score:-4.05|P-value:6.22E-5 ID:SGTC_1853|Compound:st056710|FD-Score:-4.08|P-value:5.49E-5 ID:SGTC_1875|Compound:nocodazole|FD-Score:5.66|P-value:6.58E-8 ID:SGTC_1877|Compound:wortmannin|FD-Score:7.21|P-value:8.03E-12 ID:SGTC_2077|Compound:5212015|FD-Score:4.65|P-value:7.47E-6 ID:SGTC_2090|Compound:5301565|FD-Score:4.21|P-value:4.51E-5 ID:SGTC_2108|Compound:5365811|FD-Score:-4.04|P-value:6.48E-5 ID:SGTC_2141|Compound:5621638|FD-Score:5.13|P-value:8.76E-7 ID:SGTC_2161|Compound:5662504|FD-Score:3.66|P-value:3.39E-4 ID:SGTC_2200|Compound:6600604|FD-Score:4.06|P-value:7.96E-5 ID:SGTC_2202|Compound:7226349|FD-Score:3.75|P-value:2.46E-4 ID:SGTC_2322|Compound:7413319|FD-Score:5.10|P-value:1.02E-6 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:5.57|P-value:1.02E-7 ID:SGTC_2826|Compound:7992541|FD-Score:3.39|P-value:8.21E-4 ID:SGTC_2909|Compound:7941780|FD-Score:3.91|P-value:1.37E-4 ID:SGTC_2913|Compound:7966556|FD-Score:-4.90|P-value:1.80E-6 ID:SGTC_2916|Compound:7875993|FD-Score:-7.10|P-value:1.08E-11 ID:SGTC_2989|Compound:9073649|FD-Score:3.96|P-value:1.15E-4 ID:SGTC_3018|Compound:9082602|FD-Score:-5.24|P-value:3.59E-7 ID:SGTC_3136|Compound:9095672|FD-Score:3.42|P-value:7.48E-4 ID:SGTC_3152|Compound:9099253|FD-Score:-4.28|P-value:2.52E-5 ID:SGTC_3201|Compound:9113999|FD-Score:5.85|P-value:2.48E-8

Top fitness defect scores for YDR195W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1877 wortmannin 7.21 25.0 µM 8.03E-12 31 2.70E-13
2 SGTC_1687 st020778 7.01 55.4 µM 3.00E-11 87 1.21E-12
3 SGTC_3201 9113999 5.85 49.5 µM 2.48E-8 6 2.53E-9
4 SGTC_1875 nocodazole 5.66 6.0 µM 6.58E-8 tubulin folding & SWR complex 33 7.66E-9
5 SGTC_2577 2',4'-dihydroxy-4-methoxychalcone 5.57 15.3 µM 1.02E-7 9 1.26E-8
6 SGTC_2141 5621638 5.13 7.7 µM 8.76E-7 tubulin folding & SWR complex 16 1.46E-7
7 SGTC_2322 7413319 5.10 38.2 µM 1.02E-6 ERAD & cell cycle 90 1.73E-7
8 SGTC_691 1548-0686 4.91 185.0 µM 2.35E-6 16 4.47E-7
9 SGTC_2077 5212015 4.65 200.0 µM 7.47E-6 42 1.67E-6
10 SGTC_682 0kpi-0099 4.62 17.0 µM 8.38E-6 48 1.90E-6
11 SGTC_168 3970-0862 4.23 530.5 µM 4.20E-5 103 1.19E-5
12 SGTC_2090 5301565 4.21 104.5 µM 4.51E-5 TSC3-RPN4 39 1.29E-5
13 SGTC_2200 6600604 4.06 21.9 µM 7.96E-5 tubulin folding & SWR complex 51 2.46E-5
14 SGTC_241 5hr heat shock (37°C) + cantharidin 4.04 155.4 µM 8.72E-5 cell wall signaling 70 2.73E-5
15 SGTC_800 3448-7362 4.02 155.0 µM 9.35E-5 translation 155 2.95E-5
16 SGTC_2989 9073649 3.96 71.4 µM 1.15E-4 38 3.73E-5
17 SGTC_2909 7941780 3.91 10.5 µM 1.37E-4 21 4.56E-5
18 SGTC_43 0570-0105 3.83 72.5 µM 1.87E-4 ERG2 102 6.48E-5
19 SGTC_2202 7226349 3.75 200.0 µM 2.46E-4 10 8.86E-5
20 SGTC_229 benomyl 3.74 22.9 µM 2.51E-4 tubulin folding & SWR complex 36 9.06E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.221 1.66E-38 YGR164W_d YGR164W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.187 7.78E-28 YLR087C CSF1 hom Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.171 2.35E-23 YGL012W ERG4 hom C-24(28) sterol reductase, catalyzes the final step in ergosterol biosynthesis; mutants are viable, but lack ergosterol
0.169 6.65E-23 YLR204W QRI5 hom Mitochondrial inner membrane protein, required for accumulation of spliced COX1 mRNA; may have an additional role in translation of COX1 mRNA
0.168 1.23E-22 YDR120C TRM1 hom tRNA methyltransferase; two forms of the protein are made by alternative translation starts; localizes to both the nucleus and mitochondrion to produce the modified base N2,N2-dimethylguanosine in tRNAs in both compartments
0.167 2.86E-22 YBL079W NUP170 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication
0.164 1.15E-21 YJR074W MOG1 hom Conserved nuclear protein that interacts with GTP-Gsp1p, which is a Ran homolog of the Ras GTPase family, and stimulates nucleotide release, involved in nuclear protein import, nucleotide release is inhibited by Yrb1p
0.159 1.70E-20 YLR268W SEC22 hom R-SNARE protein; assembles into SNARE complex with Bet1p, Bos1p and Sed5p; cycles between the ER and Golgi complex; involved in anterograde and retrograde transport between the ER and Golgi; synaptobrevin homolog
0.159 2.45E-20 YGL094C PAN2 hom Essential subunit of the Pan2p-Pan3p poly(A)-ribonuclease complex, which acts to control poly(A) tail length and regulate the stoichiometry and activity of postreplication repair complexes
0.153 6.00E-19 YPR070W MED1 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
0.149 4.81E-18 YGL212W VAM7 hom Vacuolar SNARE protein; functions with Vam3p in vacuolar protein trafficking; has an N-terminal PX domain (phosphoinositide-binding module) that binds PtdIns-3-P and mediates membrane binding; SNAP-25 homolog; protein abundance increases in response to DNA replication stress
0.148 6.11E-18 YFL025C BST1 hom GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules
0.145 3.24E-17 YML001W YPT7 hom Rab family GTPase; GTP-binding protein of the rab family; required for homotypic fusion event in vacuole inheritance, for endosome-endosome fusion; interacts with the cargo selection/retromer complex for retrograde sorting; similar to mammalian Rab7
0.144 4.11E-17 YPL042C SSN3 hom Cyclin-dependent protein kinase, component of RNA polymerase II holoenzyme; involved in phosphorylation of the RNA polymerase II C-terminal domain; involved in glucose repression
0.141 2.32E-16 YER151C UBP3 hom Ubiquitin-specific protease involved in transport and osmotic response; interacts with Bre5p to co-regulate anterograde and retrograde transport between the ER and Golgi; involved in transcription elongation in response to osmostress through phosphorylation at Ser695 by Hog1p; inhibitor of gene silencing; cleaves ubiquitin fusions but not polyubiquitin; also has mRNA binding activity; protein abundance increases in response to DNA replication stress