YDR197W / CBS2

Mitochondrial translational activator of the COB mRNA; interacts with translating ribosomes, acts on the COB mRNA 5'-untranslated leader

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR197W / CBS2

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:5.23|P-value:2.92E-6 ID:SGTC_34|Compound:0469-0683|FD-Score:4.55|P-value:4.00E-5 ID:SGTC_134|Compound:0269-0018|FD-Score:5.23|P-value:2.93E-6 ID:SGTC_220|Compound:paclitaxel|FD-Score:4.07|P-value:2.11E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:20.80|P-value:1.54E-72 ID:SGTC_310|Compound:1273-0058|FD-Score:4.62|P-value:3.09E-5 ID:SGTC_357|Compound:1325-0123|FD-Score:-4.11|P-value:1.94E-4 ID:SGTC_386|Compound:1509-0011|FD-Score:13.40|P-value:2.40E-31 ID:SGTC_558|Compound:k064-0027|FD-Score:-4.14|P-value:1.74E-4 ID:SGTC_773|Compound:4358-1457|FD-Score:3.81|P-value:4.71E-4 ID:SGTC_852|Compound:0418-0143|FD-Score:3.64|P-value:7.96E-4 ID:SGTC_1233|Compound:0325-0408|FD-Score:3.90|P-value:3.57E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-5.46|P-value:1.22E-6 ID:SGTC_1463|Compound:k081-0032|FD-Score:-5.49|P-value:1.05E-6 ID:SGTC_1635|Compound:st007035|FD-Score:17.90|P-value:1.14E-54 ID:SGTC_1637|Compound:st007924|FD-Score:-4.06|P-value:2.32E-4 ID:SGTC_1840|Compound:st055363|FD-Score:4.24|P-value:1.18E-4 ID:SGTC_1977|Compound:st070720|FD-Score:-6.37|P-value:1.86E-8 ID:SGTC_1987|Compound:st072290|FD-Score:-4.55|P-value:4.25E-5 ID:SGTC_2394|Compound:5221648|FD-Score:3.94|P-value:3.22E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:8.21|P-value:5.78E-13 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:4.39|P-value:6.98E-5 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:5.18|P-value:3.64E-6 ID:SGTC_2606|Compound:farnesol|FD-Score:3.58|P-value:9.53E-4 ID:SGTC_2607|Compound:solasodine|FD-Score:5.96|P-value:1.19E-7 ID:SGTC_2611|Compound:pyromycin|FD-Score:5.18|P-value:3.59E-6 ID:SGTC_2942|Compound:9052777|FD-Score:5.23|P-value:2.89E-6 ID:SGTC_2943|Compound:9068529|FD-Score:3.58|P-value:9.64E-4 ID:SGTC_2946|Compound:9065502|FD-Score:-5.53|P-value:9.14E-7 ID:SGTC_3202|Compound:9113761|FD-Score:-6.32|P-value:2.35E-8 ID:SGTC_3204|Compound:9114183|FD-Score:-5.03|P-value:7.09E-6 ID:SGTC_3205|Compound:9113483|FD-Score:-4.81|P-value:1.64E-5 ID:SGTC_3209|Compound:9113616|FD-Score:4.69|P-value:2.44E-5 ID:SGTC_3211|Compound:9128781|FD-Score:10.30|P-value:2.24E-19 ID:SGTC_3285|Compound:9112818|FD-Score:3.63|P-value:8.24E-4 ID:SGTC_3302|Compound:9125395|FD-Score:4.07|P-value:2.12E-4 ID:SGTC_3310|Compound:9130229|FD-Score:3.65|P-value:7.71E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:5.23|P-value:2.92E-6 ID:SGTC_34|Compound:0469-0683|FD-Score:4.55|P-value:4.00E-5 ID:SGTC_134|Compound:0269-0018|FD-Score:5.23|P-value:2.93E-6 ID:SGTC_220|Compound:paclitaxel|FD-Score:4.07|P-value:2.11E-4 ID:SGTC_236|Compound:1509-0011|FD-Score:20.80|P-value:1.54E-72 ID:SGTC_310|Compound:1273-0058|FD-Score:4.62|P-value:3.09E-5 ID:SGTC_357|Compound:1325-0123|FD-Score:-4.11|P-value:1.94E-4 ID:SGTC_386|Compound:1509-0011|FD-Score:13.40|P-value:2.40E-31 ID:SGTC_558|Compound:k064-0027|FD-Score:-4.14|P-value:1.74E-4 ID:SGTC_773|Compound:4358-1457|FD-Score:3.81|P-value:4.71E-4 ID:SGTC_852|Compound:0418-0143|FD-Score:3.64|P-value:7.96E-4 ID:SGTC_1233|Compound:0325-0408|FD-Score:3.90|P-value:3.57E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-5.46|P-value:1.22E-6 ID:SGTC_1463|Compound:k081-0032|FD-Score:-5.49|P-value:1.05E-6 ID:SGTC_1635|Compound:st007035|FD-Score:17.90|P-value:1.14E-54 ID:SGTC_1637|Compound:st007924|FD-Score:-4.06|P-value:2.32E-4 ID:SGTC_1840|Compound:st055363|FD-Score:4.24|P-value:1.18E-4 ID:SGTC_1977|Compound:st070720|FD-Score:-6.37|P-value:1.86E-8 ID:SGTC_1987|Compound:st072290|FD-Score:-4.55|P-value:4.25E-5 ID:SGTC_2394|Compound:5221648|FD-Score:3.94|P-value:3.22E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:8.21|P-value:5.78E-13 ID:SGTC_2542|Compound:2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene|FD-Score:4.39|P-value:6.98E-5 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:5.18|P-value:3.64E-6 ID:SGTC_2606|Compound:farnesol|FD-Score:3.58|P-value:9.53E-4 ID:SGTC_2607|Compound:solasodine|FD-Score:5.96|P-value:1.19E-7 ID:SGTC_2611|Compound:pyromycin|FD-Score:5.18|P-value:3.59E-6 ID:SGTC_2942|Compound:9052777|FD-Score:5.23|P-value:2.89E-6 ID:SGTC_2943|Compound:9068529|FD-Score:3.58|P-value:9.64E-4 ID:SGTC_2946|Compound:9065502|FD-Score:-5.53|P-value:9.14E-7 ID:SGTC_3202|Compound:9113761|FD-Score:-6.32|P-value:2.35E-8 ID:SGTC_3204|Compound:9114183|FD-Score:-5.03|P-value:7.09E-6 ID:SGTC_3205|Compound:9113483|FD-Score:-4.81|P-value:1.64E-5 ID:SGTC_3209|Compound:9113616|FD-Score:4.69|P-value:2.44E-5 ID:SGTC_3211|Compound:9128781|FD-Score:10.30|P-value:2.24E-19 ID:SGTC_3285|Compound:9112818|FD-Score:3.63|P-value:8.24E-4 ID:SGTC_3302|Compound:9125395|FD-Score:4.07|P-value:2.12E-4 ID:SGTC_3310|Compound:9130229|FD-Score:3.65|P-value:7.71E-4

Top fitness defect scores for YDR197W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_236 1509-0011 20.80 5.3 µM 1.54E-72 48 4.04E-96
2 SGTC_1635 st007035 17.90 42.4 µM 1.14E-54 24 2.86E-72
3 SGTC_386 1509-0011 13.40 3.1 µM 2.40E-31 28 3.67E-41
4 SGTC_3211 9128781 10.30 49.5 µM 2.24E-19 6 3.24E-25
5 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 8.21 3.7 µM 5.78E-13 2 1.10E-16
6 SGTC_2607 solasodine 5.96 47.4 µM 1.19E-7 29 1.23E-9
7 SGTC_2942 9052777 5.23 62.2 µM 2.89E-6 8 8.34E-8
8 SGTC_6 0844-0013 5.23 19.8 µM 2.92E-6 30 8.47E-8
9 SGTC_134 0269-0018 5.23 6.9 µM 2.93E-6 100 8.49E-8
10 SGTC_2611 pyromycin 5.18 23.9 µM 3.59E-6 7 1.11E-7
11 SGTC_2604 4'-hydroxychalcone 5.18 11.3 µM 3.64E-6 14 1.13E-7
12 SGTC_3209 9113616 4.69 49.5 µM 2.44E-5 11 1.40E-6
13 SGTC_310 1273-0058 4.62 1.1 µM 3.09E-5 mitochondrial stress 201 1.90E-6
14 SGTC_34 0469-0683 4.55 81.8 µM 4.00E-5 SWF1 & branched chain AA biosynthesis 18 2.68E-6
15 SGTC_2542 2-hydroxy-5 (6)epoxy-tetrahydrocaryophyllene 4.39 100.0 µM 6.98E-5 10 5.56E-6
16 SGTC_1840 st055363 4.24 71.0 µM 1.18E-4 7 1.11E-5
17 SGTC_220 paclitaxel 4.07 373.4 µM 2.11E-4 7 2.37E-5
18 SGTC_3302 9125395 4.07 16.4 µM 2.12E-4 6 2.40E-5
19 SGTC_2394 5221648 3.94 200.0 µM 3.22E-4 22 4.14E-5
20 SGTC_1233 0325-0408 3.90 14.2 µM 3.57E-4 20 4.75E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.637 0 YJL166W QCR8 hom Subunit 8 of ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the intermembrane space; expression is regulated by Abf1p and Cpf1p
0.615 0 YDR230W_d YDR230W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene COX20
0.602 0 YJL022W_d YJL022W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene PET130
0.594 8.90E-319 YMR151W_d YIM2_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 5% of ORF overlaps the verified gene IMP1
0.592 3.53E-317 YPL013C MRPS16 hom Mitochondrial ribosomal protein of the small subunit
0.584 4.81E-306 YPL132W COX11 hom Mitochondrial inner membrane protein required for delivery of copper to the Cox1p subunit of cytochrome c oxidase; association with mitochondrial ribosomes suggests that copper delivery may occur during translation of Cox1p
0.569 1.47E-287 YPL215W CBP3 hom Mitochondrial protein required for assembly of cytochrome bc1 complex; forms a complex with Cbp6p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.568 1.04E-285 YDR375C BCS1 hom Protein translocase and chaperone required for Complex III assembly; member of the AAA ATPase family; forms a homo-oligomeric complex in the mitochondrial inner membrane that translocates the C-terminal domain of Rip1p from the matrix across the inner membrane, and then delivers it to an assembly intermediate of respiratory Complex III in an ATP-dependent reaction; also required for assembly of the Qcr10p subunit; mutations in human homolog BCS1L linked to neonatal diseases
0.562 4.99E-279 YEL059C-A SOM1 hom Subunit of the mitochondrial inner membrane peptidase, which is required for maturation of mitochondrial proteins of the intermembrane space; Som1p facilitates cleavage of a subset of substrates; contains twin cysteine-x9-cysteine motifs
0.556 8.22E-271 YOR330C MIP1 hom Mitochondrial DNA polymerase; conserved C-terminal segment is required for the maintenance of mitochondrial genome; mutations in the human ortholog POLG are associated with Alpers-Huttenlocher syndrome (AHS) and other mitochondrial diseases; Mip1p is the single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoans in which there is a complex of a catalytic subunit and an accessory subunit
0.555 7.47E-271 YHR116W COX23 hom Mitochondrial intermembrane space protein that functions in mitochondrial copper homeostasis, essential for functional cytochrome oxidase expression; homologous to Cox17p; contains twin cysteine-x9-cysteine motifs
0.552 1.65E-267 YJL062W-A COA3 hom Mitochondrial inner membrane protein that participates in regulation of COX1 translation, Cox1p stabilization, and cytochrome oxidase assembly
0.552 8.88E-267 YLL009C COX17 hom Copper metallochaperone that transfers copper to Sco1p and Cox11p for eventual delivery to cytochrome c oxidase; contains twin cysteine-x9-cysteine motifs
0.551 4.17E-266 YER058W PET117 hom Protein required for assembly of cytochrome c oxidase
0.539 2.78E-252 YJR120W YJR120W hom Protein of unknown function; essential for growth under anaerobic conditions; mutation causes decreased expression of ATP2, impaired respiration, defective sterol uptake, and altered levels/localization of ABC transporters Aus1p and Pdr11p