YDR198C / RKM2

Ribosomal protein lysine methyltransferase, responsible for trimethylation of the lysine residue at position 3 of Rpl12Ap and Rpl12Bp

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR198C / RKM2

Click on Significant Values for Screen Details ID:SGTC_4|Compound:0960-0234|FD-Score:-3.76|P-value:1.22E-4 ID:SGTC_7|Compound:0192-1582|FD-Score:-3.27|P-value:7.07E-4 ID:SGTC_592|Compound:k241-0073|FD-Score:3.47|P-value:4.61E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.29|P-value:8.47E-4 ID:SGTC_1546|Compound:5-methoxyflavanone|FD-Score:3.72|P-value:1.84E-4 ID:SGTC_1836|Compound:st055642|FD-Score:-3.25|P-value:7.43E-4 ID:SGTC_1940|Compound:st075990|FD-Score:3.74|P-value:1.76E-4 ID:SGTC_1945|Compound:st075991|FD-Score:-3.95|P-value:5.94E-5 ID:SGTC_1948|Compound:st076329|FD-Score:-4.79|P-value:1.55E-6 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:3.70|P-value:1.99E-4 ID:SGTC_2013|Compound:4024628|FD-Score:3.96|P-value:7.61E-5 ID:SGTC_2094|Compound:5332512|FD-Score:-4.27|P-value:1.61E-5 ID:SGTC_2214|Compound:7179297|FD-Score:3.63|P-value:2.59E-4 ID:SGTC_2388|Compound:9080168|FD-Score:3.99|P-value:6.83E-5 ID:SGTC_2559|Compound:tetrahydrosappanone a trimethyl ether|FD-Score:3.57|P-value:3.20E-4 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:3.44|P-value:5.02E-4 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:-3.28|P-value:6.80E-4 ID:SGTC_2762|Compound:leflunomide|FD-Score:3.39|P-value:5.96E-4 ID:SGTC_2774|Compound:tamoxifen|FD-Score:3.34|P-value:7.20E-4 ID:SGTC_4|Compound:0960-0234|FD-Score:-3.76|P-value:1.22E-4 ID:SGTC_7|Compound:0192-1582|FD-Score:-3.27|P-value:7.07E-4 ID:SGTC_592|Compound:k241-0073|FD-Score:3.47|P-value:4.61E-4 ID:SGTC_1125|Compound:tnp00248|FD-Score:3.29|P-value:8.47E-4 ID:SGTC_1546|Compound:5-methoxyflavanone|FD-Score:3.72|P-value:1.84E-4 ID:SGTC_1836|Compound:st055642|FD-Score:-3.25|P-value:7.43E-4 ID:SGTC_1940|Compound:st075990|FD-Score:3.74|P-value:1.76E-4 ID:SGTC_1945|Compound:st075991|FD-Score:-3.95|P-value:5.94E-5 ID:SGTC_1948|Compound:st076329|FD-Score:-4.79|P-value:1.55E-6 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:3.70|P-value:1.99E-4 ID:SGTC_2013|Compound:4024628|FD-Score:3.96|P-value:7.61E-5 ID:SGTC_2094|Compound:5332512|FD-Score:-4.27|P-value:1.61E-5 ID:SGTC_2214|Compound:7179297|FD-Score:3.63|P-value:2.59E-4 ID:SGTC_2388|Compound:9080168|FD-Score:3.99|P-value:6.83E-5 ID:SGTC_2559|Compound:tetrahydrosappanone a trimethyl ether|FD-Score:3.57|P-value:3.20E-4 ID:SGTC_2565|Compound:isoliquiritigenin|FD-Score:3.44|P-value:5.02E-4 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:-3.28|P-value:6.80E-4 ID:SGTC_2762|Compound:leflunomide|FD-Score:3.39|P-value:5.96E-4 ID:SGTC_2774|Compound:tamoxifen|FD-Score:3.34|P-value:7.20E-4

Top fitness defect scores for YDR198C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2388 9080168 3.99 200.0 µM 6.83E-5 2 3.37E-5
2 SGTC_2013 4024628 3.96 200.0 µM 7.61E-5 40 3.78E-5
3 SGTC_1940 st075990 3.74 51.1 µM 1.76E-4 60S ribosome export 26 9.30E-5
4 SGTC_1546 5-methoxyflavanone 3.72 78.7 µM 1.84E-4 13 9.81E-5
5 SGTC_1951 sophoraflavanone G 3.70 9.2 µM 1.99E-4 RNA processing & uracil transport 33 1.06E-4
6 SGTC_2214 7179297 3.63 48.3 µM 2.59E-4 superoxide 7 1.41E-4
7 SGTC_2559 tetrahydrosappanone a trimethyl ether 3.57 100.0 µM 3.20E-4 20 1.77E-4
8 SGTC_592 k241-0073 3.47 46.5 µM 4.61E-4 31 2.63E-4
9 SGTC_2565 isoliquiritigenin 3.44 100.0 µM 5.02E-4 48 2.88E-4
10 SGTC_2762 leflunomide 3.39 42.1 µM 5.96E-4 53 3.47E-4
11 SGTC_2774 tamoxifen 3.34 10.9 µM 7.20E-4 29 4.26E-4
12 SGTC_1125 tnp00248 3.29 1.3 µM 8.47E-4 71 5.07E-4
13 SGTC_1476 4112-3294 3.23 3.3 µM 0.00102 60S ribosome export 40 6.19E-4
14 SGTC_1884 5633444 3.18 25.0 µM 0.00119 redox potentiating 91 7.34E-4
15 SGTC_1859 st055998 3.11 20.0 µM 0.00148 52 9.25E-4
16 SGTC_1941 st076221 3.08 61.8 µM 0.00163 sphingolipid biosynthesis & PDR1 134 0.00102
17 SGTC_1300 1094-0077 3.08 113.0 µM 0.00167 39 0.00105
18 SGTC_1104 tacrolimus 3.03 29.8 µM 0.00192 72 0.00122
19 SGTC_1753 st043925 3.02 64.0 µM 0.00196 39 0.00125
20 SGTC_1548 st061468 2.95 90.8 µM 0.00243 35 0.00158

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.137 1.38E-15 YDR165W TRM82 hom Catalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p
0.111 1.14E-10 YOR308C SNU66 hom Component of the U4/U6.U5 snRNP complex involved in pre-mRNA splicing via spliceosome; also required for pre-5S rRNA processing and may act in concert with Rnh70p; has homology to human SART-1
0.105 1.16E-9 YLR394W CST9 hom SUMO E3 ligase; required for synaptonemal complex formation; localizes to synapsis initiation sites on meiotic chromosomes; potential Cdc28p substrate
0.092 1.05E-7 YCL048W SPS22 hom Protein of unknown function; SPS22 has a paralog, SPS2, that arose from the whole genome duplication; redundant with Sps2p for the organization of the beta-glucan layer of the spore wall
0.086 5.51E-7 YHR130C_d YHR130C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.085 9.05E-7 YGR110W CLD1 hom Mitochondrial cardiolipin-specific phospholipase; functions upstream of Taz1p to generate monolyso-cardiolipin; transcription increases upon genotoxic stress; involved in restricting Ty1 transposition; has homology to mammalian CGI-58
0.083 1.36E-6 YDL004W ATP16 het Delta subunit of the central stalk of mitochondrial F1F0 ATP synthase, which is a large, evolutionarily conserved enzyme complex required for ATP synthesis; phosphorylated
0.083 1.64E-6 YNL024C_p YNL024C_p hom Putative methyltransferase; has seven beta-strand methyltransferase motif; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; despite similarity to methyltransferases, null mutant does not display alterations in lysine methylation pattern
0.082 1.76E-6 YDR499W LCD1 het Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress
0.082 2.20E-6 YDR272W GLO2 hom Cytoplasmic glyoxalase II, catalyzes the hydrolysis of S-D-lactoylglutathione into glutathione and D-lactate
0.082 2.28E-6 YJL051W IRC8 hom Bud tip localized protein of unknown function; mRNA is targeted to the bud by a She2p dependent transport system; mRNA is cell cycle regulated via Fkh2p, peaking in G2/M phase; null mutant displays increased levels of spontaneous Rad52p foc
0.080 3.38E-6 YML102W CAC2 hom Subunit of chromatin assembly factor I (CAF-1), with Rlf2p and Msi1p; chromatin assembly by CAF-1 is important for multiple processes including silencing at telomeres, mating type loci, and rDNA; maintenance of kinetochore structure; deactivation of the DNA damage checkpoint after DNA repair; and chromatin dynamics during transcription
0.080 3.68E-6 YMR122C_d YMR122C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.079 4.60E-6 YDR281C PHM6 hom Protein of unknown function, expression is regulated by phosphate levels
0.076 1.02E-5 YMR094W CTF13 het Subunit of the CBF3 complex, which binds to the CDE III element of centromeres, bending the DNA upon binding, and may be involved in sister chromatid cohesion during mitosis