YDR200C / VPS64

Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR200C / VPS64

Click on Significant Values for Screen Details ID:SGTC_94|Compound:0334-0113|FD-Score:3.98|P-value:1.21E-4 ID:SGTC_99|Compound:0109-0120|FD-Score:4.53|P-value:1.34E-5 ID:SGTC_103|Compound:0844-0104|FD-Score:4.12|P-value:7.10E-5 ID:SGTC_115|Compound:0527-0192|FD-Score:5.18|P-value:7.50E-7 ID:SGTC_151|Compound:3013-0144|FD-Score:-3.52|P-value:2.71E-4 ID:SGTC_152|Compound:4469-0003|FD-Score:-3.26|P-value:6.45E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:-3.79|P-value:1.01E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:3.60|P-value:4.71E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.93|P-value:5.85E-5 ID:SGTC_351|Compound:caspofungin|FD-Score:3.64|P-value:4.00E-4 ID:SGTC_352|Compound:k048-0007|FD-Score:5.00|P-value:1.74E-6 ID:SGTC_384|Compound:k007-0144|FD-Score:5.07|P-value:1.26E-6 ID:SGTC_424|Compound:splitomicin|FD-Score:-3.89|P-value:6.86E-5 ID:SGTC_426|Compound:0988-0037|FD-Score:6.57|P-value:4.32E-10 ID:SGTC_435|Compound:c-PAF|FD-Score:-3.64|P-value:1.77E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:5.04|P-value:1.46E-6 ID:SGTC_568|Compound:1193-0097|FD-Score:6.37|P-value:1.41E-9 ID:SGTC_611|Compound:3453-1652|FD-Score:3.96|P-value:1.30E-4 ID:SGTC_637|Compound:1181-0519|FD-Score:3.91|P-value:1.57E-4 ID:SGTC_642|Compound:0335-0876|FD-Score:3.63|P-value:4.26E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:10.30|P-value:2.34E-22 ID:SGTC_739|Compound:1486-1183|FD-Score:-3.60|P-value:2.04E-4 ID:SGTC_777|Compound:1315-0167|FD-Score:5.04|P-value:1.46E-6 ID:SGTC_787|Compound:4152-0075|FD-Score:4.89|P-value:2.88E-6 ID:SGTC_805|Compound:3852-0218|FD-Score:5.29|P-value:4.42E-7 ID:SGTC_835|Compound:0312-0010|FD-Score:4.17|P-value:5.76E-5 ID:SGTC_954|Compound:1189-0853|FD-Score:4.34|P-value:2.95E-5 ID:SGTC_967|Compound:1306-0166|FD-Score:5.76|P-value:3.95E-8 ID:SGTC_1026|Compound:k007-1105|FD-Score:5.42|P-value:2.27E-7 ID:SGTC_1043|Compound:0916-0009|FD-Score:6.03|P-value:9.54E-9 ID:SGTC_1124|Compound:fisetin|FD-Score:5.04|P-value:1.45E-6 ID:SGTC_1161|Compound:k007-0610|FD-Score:3.91|P-value:1.57E-4 ID:SGTC_1380|Compound:2291-0730|FD-Score:4.51|P-value:1.50E-5 ID:SGTC_1479|Compound:0549-0158|FD-Score:3.74|P-value:2.90E-4 ID:SGTC_1500|Compound:4488-1866|FD-Score:3.37|P-value:9.93E-4 ID:SGTC_1571|Compound:st070080|FD-Score:4.52|P-value:1.41E-5 ID:SGTC_1728|Compound:st035614|FD-Score:3.95|P-value:1.34E-4 ID:SGTC_1754|Compound:st042065|FD-Score:3.75|P-value:2.78E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:4.49|P-value:1.61E-5 ID:SGTC_1873|Compound:rapamycin|FD-Score:3.71|P-value:3.22E-4 ID:SGTC_1876|Compound:myriocin|FD-Score:-3.79|P-value:1.00E-4 ID:SGTC_1884|Compound:5633444|FD-Score:9.22|P-value:2.42E-18 ID:SGTC_1955|Compound:wogonin|FD-Score:-3.41|P-value:3.94E-4 ID:SGTC_2027|Compound:5150920|FD-Score:-4.25|P-value:1.60E-5 ID:SGTC_2029|Compound:5175081|FD-Score:3.63|P-value:4.25E-4 ID:SGTC_2036|Compound:5160441|FD-Score:6.90|P-value:5.57E-11 ID:SGTC_2045|Compound:5106652|FD-Score:-3.65|P-value:1.66E-4 ID:SGTC_2065|Compound:5221596|FD-Score:-4.39|P-value:9.08E-6 ID:SGTC_2066|Compound:5222451|FD-Score:5.46|P-value:1.92E-7 ID:SGTC_2069|Compound:5228469|FD-Score:-4.49|P-value:5.87E-6 ID:SGTC_2076|Compound:5212012|FD-Score:-3.95|P-value:5.40E-5 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:-4.52|P-value:5.15E-6 ID:SGTC_2177|Compound:5811945|FD-Score:3.60|P-value:4.65E-4 ID:SGTC_2271|Compound:7849410|FD-Score:4.71|P-value:6.44E-6 ID:SGTC_2322|Compound:7413319|FD-Score:3.51|P-value:6.40E-4 ID:SGTC_2391|Compound:6285546|FD-Score:4.06|P-value:9.04E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:7.59|P-value:5.92E-13 ID:SGTC_2584|Compound:androsterone|FD-Score:-3.58|P-value:2.19E-4 ID:SGTC_2638|Compound:epoxomicin|FD-Score:-3.90|P-value:6.55E-5 ID:SGTC_2693|Compound:st077581|FD-Score:-3.53|P-value:2.59E-4 ID:SGTC_2788|Compound:5282734|FD-Score:3.52|P-value:6.10E-4 ID:SGTC_2961|Compound:9083290|FD-Score:4.03|P-value:9.82E-5 ID:SGTC_2964|Compound:9083345|FD-Score:3.41|P-value:8.85E-4 ID:SGTC_94|Compound:0334-0113|FD-Score:3.98|P-value:1.21E-4 ID:SGTC_99|Compound:0109-0120|FD-Score:4.53|P-value:1.34E-5 ID:SGTC_103|Compound:0844-0104|FD-Score:4.12|P-value:7.10E-5 ID:SGTC_115|Compound:0527-0192|FD-Score:5.18|P-value:7.50E-7 ID:SGTC_151|Compound:3013-0144|FD-Score:-3.52|P-value:2.71E-4 ID:SGTC_152|Compound:4469-0003|FD-Score:-3.26|P-value:6.45E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:-3.79|P-value:1.01E-4 ID:SGTC_184|Compound:1494-0393|FD-Score:3.60|P-value:4.71E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.93|P-value:5.85E-5 ID:SGTC_351|Compound:caspofungin|FD-Score:3.64|P-value:4.00E-4 ID:SGTC_352|Compound:k048-0007|FD-Score:5.00|P-value:1.74E-6 ID:SGTC_384|Compound:k007-0144|FD-Score:5.07|P-value:1.26E-6 ID:SGTC_424|Compound:splitomicin|FD-Score:-3.89|P-value:6.86E-5 ID:SGTC_426|Compound:0988-0037|FD-Score:6.57|P-value:4.32E-10 ID:SGTC_435|Compound:c-PAF|FD-Score:-3.64|P-value:1.77E-4 ID:SGTC_524|Compound:r073-0010|FD-Score:5.04|P-value:1.46E-6 ID:SGTC_568|Compound:1193-0097|FD-Score:6.37|P-value:1.41E-9 ID:SGTC_611|Compound:3453-1652|FD-Score:3.96|P-value:1.30E-4 ID:SGTC_637|Compound:1181-0519|FD-Score:3.91|P-value:1.57E-4 ID:SGTC_642|Compound:0335-0876|FD-Score:3.63|P-value:4.26E-4 ID:SGTC_733|Compound:4069-0007|FD-Score:10.30|P-value:2.34E-22 ID:SGTC_739|Compound:1486-1183|FD-Score:-3.60|P-value:2.04E-4 ID:SGTC_777|Compound:1315-0167|FD-Score:5.04|P-value:1.46E-6 ID:SGTC_787|Compound:4152-0075|FD-Score:4.89|P-value:2.88E-6 ID:SGTC_805|Compound:3852-0218|FD-Score:5.29|P-value:4.42E-7 ID:SGTC_835|Compound:0312-0010|FD-Score:4.17|P-value:5.76E-5 ID:SGTC_954|Compound:1189-0853|FD-Score:4.34|P-value:2.95E-5 ID:SGTC_967|Compound:1306-0166|FD-Score:5.76|P-value:3.95E-8 ID:SGTC_1026|Compound:k007-1105|FD-Score:5.42|P-value:2.27E-7 ID:SGTC_1043|Compound:0916-0009|FD-Score:6.03|P-value:9.54E-9 ID:SGTC_1124|Compound:fisetin|FD-Score:5.04|P-value:1.45E-6 ID:SGTC_1161|Compound:k007-0610|FD-Score:3.91|P-value:1.57E-4 ID:SGTC_1380|Compound:2291-0730|FD-Score:4.51|P-value:1.50E-5 ID:SGTC_1479|Compound:0549-0158|FD-Score:3.74|P-value:2.90E-4 ID:SGTC_1500|Compound:4488-1866|FD-Score:3.37|P-value:9.93E-4 ID:SGTC_1571|Compound:st070080|FD-Score:4.52|P-value:1.41E-5 ID:SGTC_1728|Compound:st035614|FD-Score:3.95|P-value:1.34E-4 ID:SGTC_1754|Compound:st042065|FD-Score:3.75|P-value:2.78E-4 ID:SGTC_1785|Compound:bathophenanthroline|FD-Score:4.49|P-value:1.61E-5 ID:SGTC_1873|Compound:rapamycin|FD-Score:3.71|P-value:3.22E-4 ID:SGTC_1876|Compound:myriocin|FD-Score:-3.79|P-value:1.00E-4 ID:SGTC_1884|Compound:5633444|FD-Score:9.22|P-value:2.42E-18 ID:SGTC_1955|Compound:wogonin|FD-Score:-3.41|P-value:3.94E-4 ID:SGTC_2027|Compound:5150920|FD-Score:-4.25|P-value:1.60E-5 ID:SGTC_2029|Compound:5175081|FD-Score:3.63|P-value:4.25E-4 ID:SGTC_2036|Compound:5160441|FD-Score:6.90|P-value:5.57E-11 ID:SGTC_2045|Compound:5106652|FD-Score:-3.65|P-value:1.66E-4 ID:SGTC_2065|Compound:5221596|FD-Score:-4.39|P-value:9.08E-6 ID:SGTC_2066|Compound:5222451|FD-Score:5.46|P-value:1.92E-7 ID:SGTC_2069|Compound:5228469|FD-Score:-4.49|P-value:5.87E-6 ID:SGTC_2076|Compound:5212012|FD-Score:-3.95|P-value:5.40E-5 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:-4.52|P-value:5.15E-6 ID:SGTC_2177|Compound:5811945|FD-Score:3.60|P-value:4.65E-4 ID:SGTC_2271|Compound:7849410|FD-Score:4.71|P-value:6.44E-6 ID:SGTC_2322|Compound:7413319|FD-Score:3.51|P-value:6.40E-4 ID:SGTC_2391|Compound:6285546|FD-Score:4.06|P-value:9.04E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:7.59|P-value:5.92E-13 ID:SGTC_2584|Compound:androsterone|FD-Score:-3.58|P-value:2.19E-4 ID:SGTC_2638|Compound:epoxomicin|FD-Score:-3.90|P-value:6.55E-5 ID:SGTC_2693|Compound:st077581|FD-Score:-3.53|P-value:2.59E-4 ID:SGTC_2788|Compound:5282734|FD-Score:3.52|P-value:6.10E-4 ID:SGTC_2961|Compound:9083290|FD-Score:4.03|P-value:9.82E-5 ID:SGTC_2964|Compound:9083345|FD-Score:3.41|P-value:8.85E-4

Top fitness defect scores for YDR200C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_733 4069-0007 10.30 56.1 µM 2.34E-22 ERAD & cell cycle 13 4.82E-25
2 SGTC_1884 5633444 9.22 25.0 µM 2.42E-18 redox potentiating 14 1.48E-20
3 SGTC_2582 agaric acid 7.59 100.0 µM 5.92E-13 redox potentiating 113 1.58E-14
4 SGTC_2036 5160441 6.90 105.0 µM 5.57E-11 NEO1 35 2.58E-12
5 SGTC_426 0988-0037 6.57 14.6 µM 4.32E-10 ERAD & cell cycle 11 2.57E-11
6 SGTC_568 1193-0097 6.37 7.5 µM 1.41E-9 ERAD & cell cycle 55 9.74E-11
7 SGTC_1043 0916-0009 6.03 19.3 µM 9.54E-9 1 8.30E-10
8 SGTC_967 1306-0166 5.76 352.0 µM 3.95E-8 4 4.09E-9
9 SGTC_2066 5222451 5.46 176.0 µM 1.92E-7 heme biosynthesis & mitochondrial translocase 11 2.43E-8
10 SGTC_1026 k007-1105 5.42 30.6 µM 2.27E-7 ERAD & cell cycle 52 2.93E-8
11 SGTC_805 3852-0218 5.29 479.0 µM 4.42E-7 ERAD & cell cycle 30 6.20E-8
12 SGTC_115 0527-0192 5.18 112.0 µM 7.50E-7 29 1.12E-7
13 SGTC_384 k007-0144 5.07 5.2 µM 1.26E-6 ERAD & cell cycle 35 2.02E-7
14 SGTC_1124 fisetin 5.04 1.2 µM 1.45E-6 12 2.37E-7
15 SGTC_524 r073-0010 5.04 27.8 µM 1.46E-6 28 2.37E-7
16 SGTC_777 1315-0167 5.04 517.0 µM 1.46E-6 Golgi 14 2.37E-7
17 SGTC_352 k048-0007 5.00 5.1 µM 1.74E-6 ERAD & cell cycle 46 2.90E-7
18 SGTC_787 4152-0075 4.89 138.0 µM 2.88E-6 7 5.12E-7
19 SGTC_2271 7849410 4.71 118.6 µM 6.44E-6 8 1.27E-6
20 SGTC_99 0109-0120 4.53 622.8 µM 1.34E-5 7 2.89E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.374 3.75E-112 YIL030C SSM4 hom Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation
0.350 1.31E-97 YMR022W UBC7 hom Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
0.345 1.31E-94 YOL002C IZH2 hom Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc
0.324 7.85E-83 YDR199W_d YDR199W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity
0.293 2.62E-67 YER084W_p YER084W_p hom Protein of unknown function; expressed at both mRNA and protein levels
0.287 9.19E-65 YMR029C FAR8 hom Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p
0.269 7.67E-57 YDR186C YDR186C hom Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.265 6.03E-55 YML014W TRM9 hom tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses
0.238 1.51E-44 YMR052C-A_d YMR052C-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.237 3.92E-44 YFR008W FAR7 hom Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress
0.232 3.46E-42 YDL100C GET3 hom Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress
0.227 1.26E-40 YMR052W FAR3 hom Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
0.221 1.76E-38 YML012C-A_d YML012C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1
0.215 2.61E-36 YLR238W FAR10 hom Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate
0.213 1.37E-35 YKR014C YPT52 hom Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress