HIPHOP chemogenomics database

YDR200C / VPS64

Protein required for cytoplasm to vacuole targeting of proteins; forms a complex with Far3p and Far7p to Far11p involved in recovery from pheromone-induced cell cycle arrest; mutant has increased aneuploidy tolerance

Zygosity: Homozygous strain

fixedexpanded
Profile for YDR200C / VPS64

Click on Significant Values for Screen Details

Top fitness defect scores for YDR200C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_733	4069-0007	10.30	56.1 µM	2.34E-22	ERAD & cell cycle	13	4.82E-25
2	SGTC_1884	5633444	9.22	25.0 µM	2.42E-18	redox potentiating	14	1.48E-20
3	SGTC_2582	agaric acid	7.59	100.0 µM	5.92E-13	redox potentiating	113	1.58E-14
4	SGTC_2036	5160441	6.90	105.0 µM	5.57E-11	NEO1	35	2.58E-12
5	SGTC_426	0988-0037	6.57	14.6 µM	4.32E-10	ERAD & cell cycle	11	2.57E-11
6	SGTC_568	1193-0097	6.37	7.5 µM	1.41E-9	ERAD & cell cycle	55	9.74E-11
7	SGTC_1043	0916-0009	6.03	19.3 µM	9.54E-9		1	8.30E-10
8	SGTC_967	1306-0166	5.76	352.0 µM	3.95E-8		4	4.09E-9
9	SGTC_2066	5222451	5.46	176.0 µM	1.92E-7	heme biosynthesis & mitochondrial translocase	11	2.43E-8
10	SGTC_1026	k007-1105	5.42	30.6 µM	2.27E-7	ERAD & cell cycle	52	2.93E-8
11	SGTC_805	3852-0218	5.29	479.0 µM	4.42E-7	ERAD & cell cycle	30	6.20E-8
12	SGTC_115	0527-0192	5.18	112.0 µM	7.50E-7		29	1.12E-7
13	SGTC_384	k007-0144	5.07	5.2 µM	1.26E-6	ERAD & cell cycle	35	2.02E-7
14	SGTC_1124	fisetin	5.04	1.2 µM	1.45E-6		12	2.37E-7
15	SGTC_524	r073-0010	5.04	27.8 µM	1.46E-6		28	2.37E-7
16	SGTC_777	1315-0167	5.04	517.0 µM	1.46E-6	Golgi	14	2.37E-7
17	SGTC_352	k048-0007	5.00	5.1 µM	1.74E-6	ERAD & cell cycle	46	2.90E-7
18	SGTC_787	4152-0075	4.89	138.0 µM	2.88E-6		7	5.12E-7
19	SGTC_2271	7849410	4.71	118.6 µM	6.44E-6		8	1.27E-6
20	SGTC_99	0109-0120	4.53	622.8 µM	1.34E-5		7	2.89E-6

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.374	3.75E-112	YIL030C	SSM4	hom	Ubiquitin-protein ligase involved in ER-associated protein degradation; located in the ER/nuclear envelope; ssm4 mutation suppresses mRNA instability caused by an rna14 mutation
0.350	1.31E-97	YMR022W	UBC7	hom	Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly
0.345	1.31E-94	YOL002C	IZH2	hom	Plasma membrane protein involved in zinc homeostasis and osmotin-induced apoptosis; transcription regulated by Zap1p, zinc and fatty acid levels; similar to mammalian adiponectins; deletion increases sensitivity to elevated zinc
0.324	7.85E-83	YDR199W_d	YDR199W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene VPS64; computationally predicted to have thiol-disulfide oxidoreductase activity
0.293	2.62E-67	YER084W_p	YER084W_p	hom	Protein of unknown function; expressed at both mRNA and protein levels
0.287	9.19E-65	YMR029C	FAR8	hom	Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p
0.269	7.67E-57	YDR186C	YDR186C	hom	Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.265	6.03E-55	YML014W	TRM9	hom	tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses
0.238	1.51E-44	YMR052C-A_d	YMR052C-A_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.237	3.92E-44	YFR008W	FAR7	hom	Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress
0.232	3.46E-42	YDL100C	GET3	hom	Guanine nucleotide exchange factor for Gpa1p; amplifies G protein signaling; subunit of the GET complex, which is involved in Golgi to ER trafficking and insertion of proteins into the ER membrane; has low-level ATPase activity; protein abundance increases in response to DNA replication stress
0.227	1.26E-40	YMR052W	FAR3	hom	Protein of unknown function; involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far7p, Far8p, Far9p, Far10p, and Far11p; localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
0.221	1.76E-38	YML012C-A_d	YML012C-A_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene SEL1
0.215	2.61E-36	YLR238W	FAR10	hom	Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far8p, Far9p, and Far11p; potential Cdc28p substrate
0.213	1.37E-35	YKR014C	YPT52	hom	Rab family GTPase, similar to Ypt51p and Ypt53p and to mammalian rab5; required for vacuolar protein sorting and endocytosis; protein abundance increases in response to DNA replication stress