HIPHOP chemogenomics database

YDR211W / GCD6

Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress

Zygosity: Heterozygous strain

fixedexpanded
Profile for YDR211W / GCD6

Click on Significant Values for Screen Details

Top fitness defect scores for YDR211W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_2612	dictamnine	13.70	15.5 µM	1.06E-44	RNA pol III & RNase P/MRP	6	7.17E-43	0.69
2	SGTC_4	0960-0234	6.79	25.7 µM	1.99E-12	Golgi	3	5.66E-12	1.37
3	SGTC_2411	st077133	6.75	39.9 µM	2.67E-12	RNA pol III & RNase P/MRP	11	7.49E-12	0.81
4	SGTC_392	N-ethylmaleimide	6.48	8.3 µM	1.80E-11	RNA pol III & RNase P/MRP	4	4.65E-11	0.93
5	SGTC_1035	k081-0041	6.22	55.2 µM	1.06E-10	Golgi	2	2.55E-10	0.49
6	SGTC_3	0833-0587	5.94	95.6 µM	6.56E-10	redox potentiating	4	1.46E-9	0.76
7	SGTC_1031	k035-0009	5.90	16.0 µM	8.24E-10		1	1.82E-9	2.28
8	SGTC_2504	purpurogallin	5.63	52.3 µM	4.30E-9	RPP1 & pyrimidine depletion	1	8.84E-9	1.46
9	SGTC_2	0422-0095	5.36	29.0 µM	2.20E-8	redox potentiating	2	4.22E-8	1.25
10	SGTC_9	0092-0002	5.01	10.4 µM	1.56E-7		3	2.77E-7	0.17
11	SGTC_145	3737-0113	4.81	38.4 µM	4.53E-7	DNA intercalators	31	7.68E-7	0.01
12	SGTC_1145	3546-0597	4.34	10.2 µM	4.60E-6	mitochondrial processes	2	7.08E-6	0.67
13	SGTC_87	0100-0052	4.21	35.5 µM	8.52E-6		15	1.28E-5	0.14
14	SGTC_1332	1480-0653	4.18	17.5 µM	9.57E-6	TSC3-RPN4	3	1.43E-5	0.30
15	SGTC_682	0kpi-0099	4.06	17.0 µM	1.69E-5		27	2.47E-5	0.07
16	SGTC_1675	st018485	3.94	24.5 µM	2.89E-5	60S ribosome export	10	4.13E-5	0.13
17	SGTC_1926	methyl fluorone black	3.93	53.6 µM	2.92E-5	DNA intercalators	28	4.18E-5	0.10
18	SGTC_594	1487-1584	3.88	10.8 µM	3.71E-5		9	5.25E-5	0.16
19	SGTC_2121	5257820	3.80	149.6 µM	5.25E-5		7	7.33E-5	0.61
20	SGTC_5	0352-0601	3.77	109.9 µM	5.77E-5	ERG2	2	8.02E-5	2.20E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.331	1.22E-86	YLR291C	GCD7	het	Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.310	1.86E-75	YGR083C	GCD2	het	Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.293	3.08E-67	YNL221C	POP1	het	Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
0.268	2.75E-56	YOR260W	GCD1	het	Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.197	1.45E-30	YMR283C	RIT1	hom	2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA
0.183	1.34E-26	YOR116C	RPO31	het	RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
0.178	1.99E-25	YIL144W	TID3	het	Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering
0.172	1.40E-23	YDL150W	RPC53	het	RNA polymerase III subunit C53
0.159	1.58E-20	YOR207C	RET1	het	Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
0.158	4.63E-20	YML047W-A_d	YML047W-A_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.136	2.25E-15	YBR257W	POP4	het	Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
0.136	2.60E-15	YDL152W_d	YDL152W_d	het	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
0.130	3.86E-14	YHR149C	SKG6	hom	Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p
0.120	3.26E-12	YCL031C	RRP7	het	Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress
0.115	2.26E-11	YGR090W	UTP22	het	Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals