YDR211W / GCD6

Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR211W / GCD6

Click on Significant Values for Screen Details ID:SGTC_2|Compound:0422-0095|FD-Score:5.36|P-value:2.20E-8 ID:SGTC_3|Compound:0833-0587|FD-Score:5.94|P-value:6.56E-10 ID:SGTC_4|Compound:0960-0234|FD-Score:6.79|P-value:1.99E-12 ID:SGTC_5|Compound:0352-0601|FD-Score:3.77|P-value:5.77E-5 ID:SGTC_9|Compound:0092-0002|FD-Score:5.01|P-value:1.56E-7 ID:SGTC_51|Compound:1013-0126|FD-Score:-3.62|P-value:1.06E-4 ID:SGTC_62|Compound:0180-0423|FD-Score:3.19|P-value:5.66E-4 ID:SGTC_72|Compound:0744-0168|FD-Score:3.21|P-value:5.28E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:4.21|P-value:8.52E-6 ID:SGTC_126|Compound:0417-1624|FD-Score:3.06|P-value:8.74E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:4.81|P-value:4.53E-7 ID:SGTC_292|Compound:k038-0015|FD-Score:3.42|P-value:2.41E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:3.06|P-value:8.91E-4 ID:SGTC_385|Compound:1494-0393|FD-Score:3.07|P-value:8.53E-4 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:6.48|P-value:1.80E-11 ID:SGTC_594|Compound:1487-1584|FD-Score:3.88|P-value:3.71E-5 ID:SGTC_611|Compound:3453-1652|FD-Score:3.64|P-value:1.02E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.06|P-value:1.69E-5 ID:SGTC_734|Compound:2023-0417|FD-Score:3.35|P-value:3.09E-4 ID:SGTC_777|Compound:1315-0167|FD-Score:-3.24|P-value:4.58E-4 ID:SGTC_781|Compound:4335-2806|FD-Score:-4.66|P-value:9.11E-7 ID:SGTC_791|Compound:1348-1292|FD-Score:3.08|P-value:8.42E-4 ID:SGTC_796|Compound:1234-0020|FD-Score:3.76|P-value:6.18E-5 ID:SGTC_869|Compound:0564-0080|FD-Score:3.07|P-value:8.50E-4 ID:SGTC_962|Compound:1254-0300|FD-Score:-3.40|P-value:2.48E-4 ID:SGTC_963|Compound:1269-2853|FD-Score:-3.56|P-value:1.32E-4 ID:SGTC_1031|Compound:k035-0009|FD-Score:5.90|P-value:8.24E-10 ID:SGTC_1035|Compound:k081-0041|FD-Score:6.22|P-value:1.06E-10 ID:SGTC_1038|Compound:k292-0204|FD-Score:3.38|P-value:2.78E-4 ID:SGTC_1044|Compound:1222-0021|FD-Score:-3.30|P-value:3.73E-4 ID:SGTC_1145|Compound:3546-0597|FD-Score:4.34|P-value:4.60E-6 ID:SGTC_1332|Compound:1480-0653|FD-Score:4.18|P-value:9.57E-6 ID:SGTC_1449|Compound:4112-4002|FD-Score:3.76|P-value:6.06E-5 ID:SGTC_1523|Compound:st012844|FD-Score:3.17|P-value:6.02E-4 ID:SGTC_1527|Compound:coumarin|FD-Score:-5.55|P-value:7.02E-9 ID:SGTC_1530|Compound:rhein|FD-Score:3.26|P-value:4.28E-4 ID:SGTC_1668|Compound:st014914|FD-Score:3.08|P-value:8.35E-4 ID:SGTC_1675|Compound:st018485|FD-Score:3.94|P-value:2.89E-5 ID:SGTC_1711|Compound:st032263|FD-Score:3.29|P-value:3.96E-4 ID:SGTC_1726|Compound:st035556|FD-Score:3.04|P-value:9.67E-4 ID:SGTC_1872|Compound:amphotericin b|FD-Score:3.53|P-value:1.53E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:3.93|P-value:2.92E-5 ID:SGTC_2121|Compound:5257820|FD-Score:3.80|P-value:5.25E-5 ID:SGTC_2164|Compound:5685496|FD-Score:-3.49|P-value:1.81E-4 ID:SGTC_2301|Compound:7769476|FD-Score:-3.54|P-value:1.44E-4 ID:SGTC_2326|Compound:6164455|FD-Score:-3.28|P-value:3.97E-4 ID:SGTC_2411|Compound:st077133|FD-Score:6.75|P-value:2.67E-12 ID:SGTC_2431|Compound:5490079|FD-Score:-3.61|P-value:1.09E-4 ID:SGTC_2434|Compound:5670830|FD-Score:-3.54|P-value:1.47E-4 ID:SGTC_2504|Compound:purpurogallin|FD-Score:5.63|P-value:4.30E-9 ID:SGTC_2522|Compound:biochanin a|FD-Score:3.18|P-value:5.89E-4 ID:SGTC_2612|Compound:dictamnine|FD-Score:13.70|P-value:1.06E-44 ID:SGTC_2942|Compound:9052777|FD-Score:-3.85|P-value:4.16E-5 ID:SGTC_2943|Compound:9068529|FD-Score:-3.84|P-value:4.22E-5 ID:SGTC_2945|Compound:9067681|FD-Score:-5.64|P-value:3.90E-9 ID:SGTC_2970|Compound:9090938|FD-Score:3.12|P-value:7.16E-4 ID:SGTC_2|Compound:0422-0095|FD-Score:5.36|P-value:2.20E-8 ID:SGTC_3|Compound:0833-0587|FD-Score:5.94|P-value:6.56E-10 ID:SGTC_4|Compound:0960-0234|FD-Score:6.79|P-value:1.99E-12 ID:SGTC_5|Compound:0352-0601|FD-Score:3.77|P-value:5.77E-5 ID:SGTC_9|Compound:0092-0002|FD-Score:5.01|P-value:1.56E-7 ID:SGTC_51|Compound:1013-0126|FD-Score:-3.62|P-value:1.06E-4 ID:SGTC_62|Compound:0180-0423|FD-Score:3.19|P-value:5.66E-4 ID:SGTC_72|Compound:0744-0168|FD-Score:3.21|P-value:5.28E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:4.21|P-value:8.52E-6 ID:SGTC_126|Compound:0417-1624|FD-Score:3.06|P-value:8.74E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:4.81|P-value:4.53E-7 ID:SGTC_292|Compound:k038-0015|FD-Score:3.42|P-value:2.41E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:3.06|P-value:8.91E-4 ID:SGTC_385|Compound:1494-0393|FD-Score:3.07|P-value:8.53E-4 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:6.48|P-value:1.80E-11 ID:SGTC_594|Compound:1487-1584|FD-Score:3.88|P-value:3.71E-5 ID:SGTC_611|Compound:3453-1652|FD-Score:3.64|P-value:1.02E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.06|P-value:1.69E-5 ID:SGTC_734|Compound:2023-0417|FD-Score:3.35|P-value:3.09E-4 ID:SGTC_777|Compound:1315-0167|FD-Score:-3.24|P-value:4.58E-4 ID:SGTC_781|Compound:4335-2806|FD-Score:-4.66|P-value:9.11E-7 ID:SGTC_791|Compound:1348-1292|FD-Score:3.08|P-value:8.42E-4 ID:SGTC_796|Compound:1234-0020|FD-Score:3.76|P-value:6.18E-5 ID:SGTC_869|Compound:0564-0080|FD-Score:3.07|P-value:8.50E-4 ID:SGTC_962|Compound:1254-0300|FD-Score:-3.40|P-value:2.48E-4 ID:SGTC_963|Compound:1269-2853|FD-Score:-3.56|P-value:1.32E-4 ID:SGTC_1031|Compound:k035-0009|FD-Score:5.90|P-value:8.24E-10 ID:SGTC_1035|Compound:k081-0041|FD-Score:6.22|P-value:1.06E-10 ID:SGTC_1038|Compound:k292-0204|FD-Score:3.38|P-value:2.78E-4 ID:SGTC_1044|Compound:1222-0021|FD-Score:-3.30|P-value:3.73E-4 ID:SGTC_1145|Compound:3546-0597|FD-Score:4.34|P-value:4.60E-6 ID:SGTC_1332|Compound:1480-0653|FD-Score:4.18|P-value:9.57E-6 ID:SGTC_1449|Compound:4112-4002|FD-Score:3.76|P-value:6.06E-5 ID:SGTC_1523|Compound:st012844|FD-Score:3.17|P-value:6.02E-4 ID:SGTC_1527|Compound:coumarin|FD-Score:-5.55|P-value:7.02E-9 ID:SGTC_1530|Compound:rhein|FD-Score:3.26|P-value:4.28E-4 ID:SGTC_1668|Compound:st014914|FD-Score:3.08|P-value:8.35E-4 ID:SGTC_1675|Compound:st018485|FD-Score:3.94|P-value:2.89E-5 ID:SGTC_1711|Compound:st032263|FD-Score:3.29|P-value:3.96E-4 ID:SGTC_1726|Compound:st035556|FD-Score:3.04|P-value:9.67E-4 ID:SGTC_1872|Compound:amphotericin b|FD-Score:3.53|P-value:1.53E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:3.93|P-value:2.92E-5 ID:SGTC_2121|Compound:5257820|FD-Score:3.80|P-value:5.25E-5 ID:SGTC_2164|Compound:5685496|FD-Score:-3.49|P-value:1.81E-4 ID:SGTC_2301|Compound:7769476|FD-Score:-3.54|P-value:1.44E-4 ID:SGTC_2326|Compound:6164455|FD-Score:-3.28|P-value:3.97E-4 ID:SGTC_2411|Compound:st077133|FD-Score:6.75|P-value:2.67E-12 ID:SGTC_2431|Compound:5490079|FD-Score:-3.61|P-value:1.09E-4 ID:SGTC_2434|Compound:5670830|FD-Score:-3.54|P-value:1.47E-4 ID:SGTC_2504|Compound:purpurogallin|FD-Score:5.63|P-value:4.30E-9 ID:SGTC_2522|Compound:biochanin a|FD-Score:3.18|P-value:5.89E-4 ID:SGTC_2612|Compound:dictamnine|FD-Score:13.70|P-value:1.06E-44 ID:SGTC_2942|Compound:9052777|FD-Score:-3.85|P-value:4.16E-5 ID:SGTC_2943|Compound:9068529|FD-Score:-3.84|P-value:4.22E-5 ID:SGTC_2945|Compound:9067681|FD-Score:-5.64|P-value:3.90E-9 ID:SGTC_2970|Compound:9090938|FD-Score:3.12|P-value:7.16E-4

Top fitness defect scores for YDR211W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2612 dictamnine 13.70 15.5 µM 1.06E-44 RNA pol III & RNase P/MRP 6 7.17E-43 0.69
2 SGTC_4 0960-0234 6.79 25.7 µM 1.99E-12 Golgi 3 5.66E-12 1.37
3 SGTC_2411 st077133 6.75 39.9 µM 2.67E-12 RNA pol III & RNase P/MRP 11 7.49E-12 0.81
4 SGTC_392 N-ethylmaleimide 6.48 8.3 µM 1.80E-11 RNA pol III & RNase P/MRP 4 4.65E-11 0.93
5 SGTC_1035 k081-0041 6.22 55.2 µM 1.06E-10 Golgi 2 2.55E-10 0.49
6 SGTC_3 0833-0587 5.94 95.6 µM 6.56E-10 redox potentiating 4 1.46E-9 0.76
7 SGTC_1031 k035-0009 5.90 16.0 µM 8.24E-10 1 1.82E-9 2.28
8 SGTC_2504 purpurogallin 5.63 52.3 µM 4.30E-9 RPP1 & pyrimidine depletion 1 8.84E-9 1.46
9 SGTC_2 0422-0095 5.36 29.0 µM 2.20E-8 redox potentiating 2 4.22E-8 1.25
10 SGTC_9 0092-0002 5.01 10.4 µM 1.56E-7 3 2.77E-7 0.17
11 SGTC_145 3737-0113 4.81 38.4 µM 4.53E-7 DNA intercalators 31 7.68E-7 0.01
12 SGTC_1145 3546-0597 4.34 10.2 µM 4.60E-6 mitochondrial processes 2 7.08E-6 0.67
13 SGTC_87 0100-0052 4.21 35.5 µM 8.52E-6 15 1.28E-5 0.14
14 SGTC_1332 1480-0653 4.18 17.5 µM 9.57E-6 TSC3-RPN4 3 1.43E-5 0.30
15 SGTC_682 0kpi-0099 4.06 17.0 µM 1.69E-5 27 2.47E-5 0.07
16 SGTC_1675 st018485 3.94 24.5 µM 2.89E-5 60S ribosome export 10 4.13E-5 0.13
17 SGTC_1926 methyl fluorone black 3.93 53.6 µM 2.92E-5 DNA intercalators 28 4.18E-5 0.10
18 SGTC_594 1487-1584 3.88 10.8 µM 3.71E-5 9 5.25E-5 0.16
19 SGTC_2121 5257820 3.80 149.6 µM 5.25E-5 7 7.33E-5 0.61
20 SGTC_5 0352-0601 3.77 109.9 µM 5.77E-5 ERG2 2 8.02E-5 2.20E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.331 1.22E-86 YLR291C GCD7 het Beta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.310 1.86E-75 YGR083C GCD2 het Delta subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.293 3.08E-67 YNL221C POP1 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
0.268 2.75E-56 YOR260W GCD1 het Gamma subunit of the translation initiation factor eIF2B, the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression
0.197 1.45E-30 YMR283C RIT1 hom 2'-O-ribosyl phosphate transferase, modifies the initiator methionine tRNA at position 64 to distinguish it from elongator methionine tRNA
0.183 1.34E-26 YOR116C RPO31 het RNA polymerase III largest subunit C160, part of core enzyme; similar to bacterial beta-prime subunit and to RPA190 and RPO21
0.178 1.99E-25 YIL144W TID3 het Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); conserved coiled-coil protein involved in chromosome segregation, spindle checkpoint activity, kinetochore assembly and clustering
0.172 1.40E-23 YDL150W RPC53 het RNA polymerase III subunit C53
0.159 1.58E-20 YOR207C RET1 het Second-largest subunit of RNA polymerase III, which is responsible for the transcription of tRNA and 5S RNA genes, and other low molecular weight RNAs
0.158 4.63E-20 YML047W-A_d YML047W-A_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.136 2.25E-15 YBR257W POP4 het Subunit of both RNase MRP and nuclear RNase P; RNase MRP cleaves pre-rRNA, while nuclear RNase P cleaves tRNA precursors to generate mature 5' ends and facilitates turnover of nuclear RNAs; binds to the RPR1 RNA subunit in RNase P
0.136 2.60E-15 YDL152W_d YDL152W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF SAS10/YDL153C, a component of the small ribosomal subunit processosome
0.130 3.86E-14 YHR149C SKG6 hom Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p
0.120 3.26E-12 YCL031C RRP7 het Essential protein involved in rRNA processing and ribosome biogenesis; protein abundance increases in response to DNA replication stress
0.115 2.26E-11 YGR090W UTP22 het Component of the small-subunit processome; required for nuclear export of tRNAs; may facilitate binding of Utp8p to aminoacylated tRNAs and their delivery to Los1p for export; conserved from yeast to mammals