YDR217C / RAD9

DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M; transmits checkpoint signal by activating Rad53p and Chk1p; hyperphosphorylated by Mec1p and Tel1p; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; potential Cdc28p substrate

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR217C / RAD9

Click on Significant Values for Screen Details ID:SGTC_101|Compound:0490-5701|FD-Score:-3.08|P-value:8.95E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:-3.05|P-value:9.87E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:10.10|P-value:2.98E-25 ID:SGTC_563|Compound:tetrahydrosecaminediol|FD-Score:3.34|P-value:3.19E-4 ID:SGTC_604|Compound:1000-0114|FD-Score:3.74|P-value:6.61E-5 ID:SGTC_633|Compound:0437-0093|FD-Score:3.79|P-value:5.49E-5 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.08|P-value:8.10E-4 ID:SGTC_729|Compound:4092-0585|FD-Score:4.49|P-value:2.23E-6 ID:SGTC_856|Compound:0433-0016|FD-Score:-4.10|P-value:1.58E-5 ID:SGTC_961|Compound:1252-1571|FD-Score:-4.42|P-value:3.73E-6 ID:SGTC_1032|Compound:k061-1018|FD-Score:3.06|P-value:8.86E-4 ID:SGTC_1045|Compound:1348-1485|FD-Score:4.49|P-value:2.25E-6 ID:SGTC_1054|Compound:3429-0080|FD-Score:-3.60|P-value:1.30E-4 ID:SGTC_1056|Compound:3448-1962|FD-Score:-3.07|P-value:9.31E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:3.21|P-value:5.09E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:3.92|P-value:3.12E-5 ID:SGTC_1349|Compound:flufenamic acid|FD-Score:4.14|P-value:1.20E-5 ID:SGTC_1370|Compound:2001-0092|FD-Score:4.16|P-value:1.08E-5 ID:SGTC_1372|Compound:2752-0194|FD-Score:4.02|P-value:2.02E-5 ID:SGTC_1384|Compound:3089-0840|FD-Score:-3.90|P-value:3.78E-5 ID:SGTC_1718|Compound:roemerine|FD-Score:4.03|P-value:1.95E-5 ID:SGTC_1751|Compound:st044839|FD-Score:-3.10|P-value:8.32E-4 ID:SGTC_1836|Compound:st055642|FD-Score:3.91|P-value:3.28E-5 ID:SGTC_1861|Compound:st057647|FD-Score:3.47|P-value:1.92E-4 ID:SGTC_1867|Compound:st057372|FD-Score:3.27|P-value:4.21E-4 ID:SGTC_1962|Compound:st076595|FD-Score:3.35|P-value:3.08E-4 ID:SGTC_1966|Compound:st070145|FD-Score:4.65|P-value:1.02E-6 ID:SGTC_1968|Compound:st069590|FD-Score:4.76|P-value:5.84E-7 ID:SGTC_1969|Compound:st069479|FD-Score:6.04|P-value:3.62E-10 ID:SGTC_1970|Compound:st070122|FD-Score:3.18|P-value:5.65E-4 ID:SGTC_1971|Compound:st069429|FD-Score:3.20|P-value:5.43E-4 ID:SGTC_2021|Compound:4019055|FD-Score:3.56|P-value:1.37E-4 ID:SGTC_2474|Compound:5321570|FD-Score:3.25|P-value:4.46E-4 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:-4.17|P-value:1.14E-5 ID:SGTC_2784|Compound:7567673|FD-Score:4.09|P-value:1.50E-5 ID:SGTC_2902|Compound:5787006|FD-Score:3.03|P-value:9.76E-4 ID:SGTC_3040|Compound:9091894|FD-Score:3.58|P-value:1.26E-4 ID:SGTC_3204|Compound:9114183|FD-Score:3.02|P-value:9.92E-4 ID:SGTC_3213|Compound:9129884|FD-Score:3.52|P-value:1.60E-4 ID:SGTC_101|Compound:0490-5701|FD-Score:-3.08|P-value:8.95E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:-3.05|P-value:9.87E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:10.10|P-value:2.98E-25 ID:SGTC_563|Compound:tetrahydrosecaminediol|FD-Score:3.34|P-value:3.19E-4 ID:SGTC_604|Compound:1000-0114|FD-Score:3.74|P-value:6.61E-5 ID:SGTC_633|Compound:0437-0093|FD-Score:3.79|P-value:5.49E-5 ID:SGTC_682|Compound:0kpi-0099|FD-Score:3.08|P-value:8.10E-4 ID:SGTC_729|Compound:4092-0585|FD-Score:4.49|P-value:2.23E-6 ID:SGTC_856|Compound:0433-0016|FD-Score:-4.10|P-value:1.58E-5 ID:SGTC_961|Compound:1252-1571|FD-Score:-4.42|P-value:3.73E-6 ID:SGTC_1032|Compound:k061-1018|FD-Score:3.06|P-value:8.86E-4 ID:SGTC_1045|Compound:1348-1485|FD-Score:4.49|P-value:2.25E-6 ID:SGTC_1054|Compound:3429-0080|FD-Score:-3.60|P-value:1.30E-4 ID:SGTC_1056|Compound:3448-1962|FD-Score:-3.07|P-value:9.31E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:3.21|P-value:5.09E-4 ID:SGTC_1290|Compound:0986-0249|FD-Score:3.92|P-value:3.12E-5 ID:SGTC_1349|Compound:flufenamic acid|FD-Score:4.14|P-value:1.20E-5 ID:SGTC_1370|Compound:2001-0092|FD-Score:4.16|P-value:1.08E-5 ID:SGTC_1372|Compound:2752-0194|FD-Score:4.02|P-value:2.02E-5 ID:SGTC_1384|Compound:3089-0840|FD-Score:-3.90|P-value:3.78E-5 ID:SGTC_1718|Compound:roemerine|FD-Score:4.03|P-value:1.95E-5 ID:SGTC_1751|Compound:st044839|FD-Score:-3.10|P-value:8.32E-4 ID:SGTC_1836|Compound:st055642|FD-Score:3.91|P-value:3.28E-5 ID:SGTC_1861|Compound:st057647|FD-Score:3.47|P-value:1.92E-4 ID:SGTC_1867|Compound:st057372|FD-Score:3.27|P-value:4.21E-4 ID:SGTC_1962|Compound:st076595|FD-Score:3.35|P-value:3.08E-4 ID:SGTC_1966|Compound:st070145|FD-Score:4.65|P-value:1.02E-6 ID:SGTC_1968|Compound:st069590|FD-Score:4.76|P-value:5.84E-7 ID:SGTC_1969|Compound:st069479|FD-Score:6.04|P-value:3.62E-10 ID:SGTC_1970|Compound:st070122|FD-Score:3.18|P-value:5.65E-4 ID:SGTC_1971|Compound:st069429|FD-Score:3.20|P-value:5.43E-4 ID:SGTC_2021|Compound:4019055|FD-Score:3.56|P-value:1.37E-4 ID:SGTC_2474|Compound:5321570|FD-Score:3.25|P-value:4.46E-4 ID:SGTC_2498|Compound:4'-Methoxychalcone|FD-Score:-4.17|P-value:1.14E-5 ID:SGTC_2784|Compound:7567673|FD-Score:4.09|P-value:1.50E-5 ID:SGTC_2902|Compound:5787006|FD-Score:3.03|P-value:9.76E-4 ID:SGTC_3040|Compound:9091894|FD-Score:3.58|P-value:1.26E-4 ID:SGTC_3204|Compound:9114183|FD-Score:3.02|P-value:9.92E-4 ID:SGTC_3213|Compound:9129884|FD-Score:3.52|P-value:1.60E-4

Top fitness defect scores for YDR217C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_268 camptothecin 10.10 39.6 µM 2.98E-25 DNA damage response 10 2.24E-24
2 SGTC_1969 st069479 6.04 40.1 µM 3.62E-10 2 7.80E-10
3 SGTC_1968 st069590 4.76 43.6 µM 5.84E-7 8 9.62E-7
4 SGTC_1966 st070145 4.65 16.1 µM 1.02E-6 15 1.65E-6
5 SGTC_729 4092-0585 4.49 94.1 µM 2.23E-6 17 3.49E-6
6 SGTC_1045 1348-1485 4.49 165.0 µM 2.25E-6 15 3.54E-6
7 SGTC_1370 2001-0092 4.16 44.6 µM 1.08E-5 24 1.60E-5
8 SGTC_1349 flufenamic acid 4.14 69.0 µM 1.20E-5 9 1.77E-5
9 SGTC_2784 7567673 4.09 72.7 µM 1.50E-5 iron homeostasis 23 2.20E-5
10 SGTC_1718 roemerine 4.03 11.9 µM 1.95E-5 redox potentiating 53 2.82E-5
11 SGTC_1372 2752-0194 4.02 270.0 µM 2.02E-5 17 2.93E-5
12 SGTC_1290 0986-0249 3.92 10.2 µM 3.12E-5 DNA intercalators 21 4.45E-5
13 SGTC_1836 st055642 3.91 81.0 µM 3.28E-5 17 4.67E-5
14 SGTC_633 0437-0093 3.79 97.7 µM 5.49E-5 DNA intercalators 17 7.66E-5
15 SGTC_604 1000-0114 3.74 314.0 µM 6.61E-5 26 9.17E-5
16 SGTC_3040 9091894 3.58 49.5 µM 1.26E-4 DNA intercalators 25 1.71E-4
17 SGTC_2021 4019055 3.56 173.0 µM 1.37E-4 endomembrane recycling 147 1.85E-4
18 SGTC_3213 9129884 3.52 49.5 µM 1.60E-4 31 2.15E-4
19 SGTC_1861 st057647 3.47 17.4 µM 1.92E-4 40 2.57E-4
20 SGTC_1962 st076595 3.35 64.5 µM 3.08E-4 redox potentiating 54 4.04E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.168 1.36E-22 YBR099C_d YBR099C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
0.154 2.78E-19 YBL088C TEL1 hom Protein kinase primarily involved in telomere length regulation; contributes to cell cycle checkpoint control in response to DNA damage; functionally redundant with Mec1p; regulates P-body formation induced by replication stress; homolog of human ataxia-telangiectasia mutated (ATM) gene, the gene responsible for ataxia telangiectasia (AT) (OMIM 607585)
0.151 1.76E-18 YLR135W SLX4 hom Endonuclease involved in processing DNA; acts during recombination and repair; cleaves branched structures in a complex with Slx1p; involved interstrand cross-link repair and in Rad1p/Rad10p-dependent removal of 3'-nonhomologous tails during DSBR via single-strand annealing; relative distribution to nuclear foci increases upon DNA replication stress
0.149 3.40E-18 YBR098W MMS4 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.147 1.40E-17 YPL194W DDC1 hom DNA damage checkpoint protein; part of a PCNA-like complex required for DNA damage response, required for pachytene checkpoint to inhibit cell cycle in response to unrepaired recombination intermediates; potential Cdc28p substrate; forms nuclear foci upon DNA replication stress
0.133 1.20E-14 YOL135C MED7 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation
0.128 1.08E-13 YDR386W MUS81 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.121 2.40E-12 YDL058W USO1 het Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex
0.120 3.16E-12 YDL003W MCD1 het Essential alpha-kleisin subunit of the cohesin complex; required for sister chromatid cohesion in mitosis and meiosis; apoptosis induces cleavage and translocation of a C-terminal fragment to mitochondria; expression peaks in S phase
0.117 1.27E-11 YGL175C SAE2 hom Endonuclease that processes hairpin DNA structures with the MRX complex; involved in meiotic and mitotic double-strand break repair; phosphorylated in response to DNA damage and required for normal resistance to DNA-damaging agents
0.113 4.47E-11 YDR180W SCC2 het Subunit of cohesin loading factor (Scc2p-Scc4p), a complex required for loading of cohesin complexes onto chromosomes; involved in establishing sister chromatid cohesion during DSB repair via histone H2AX; evolutionarily-conserved adherin
0.107 4.93E-10 YER089C PTC2 hom Type 2C protein phosphatase (PP2C); dephosphorylates Hog1p to limit maximal osmostress induced kinase activity; dephosphorylates Ire1p to downregulate the unfolded protein response; dephosphorylates Cdc28p; inactivates the DNA damage checkpoint; PTC2 has a paralog, PTC3, that arose from the whole genome duplication
0.107 5.19E-10 YDR076W RAD55 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad57p
0.106 6.50E-10 YDR362C TFC6 het One of six subunits of RNA polymerase III transcription initiation factor complex (TFIIIC); part of TFIIIC TauB domain that binds BoxB promoter sites of tRNA and other genes; cooperates with Tfc3p in DNA binding; human homolog is TFIIIC-110
0.101 4.05E-9 YBR143C SUP45 het Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p