HIPHOP chemogenomics database

YDR221W / GTB1

Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress

Zygosity: Homozygous strain

fixedexpanded
Profile for YDR221W / GTB1

Click on Significant Values for Screen Details

Top fitness defect scores for YDR221W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_522	1273-0010	6.66	11.6 µM	1.85E-12	60S ribosome export	2	1.35E-11
2	SGTC_2518	prenyletin	5.28	46.3 µM	1.80E-8		2	6.41E-8
3	SGTC_3300	9124615	5.18	50.5 µM	3.25E-8		4	1.11E-7
4	SGTC_1860	st056228	4.90	74.6 µM	1.57E-7		4	4.73E-7
5	SGTC_266	formosanin C	4.86	406.0 nM	2.03E-7	sphingolipid biosynthesis & PDR1	50	5.99E-7
6	SGTC_3201	9113999	4.74	49.5 µM	3.77E-7		17	1.06E-6
7	SGTC_2937	9044753	4.73	11.3 µM	4.06E-7	60S ribosome export	18	1.13E-6
8	SGTC_2526	patulin	4.61	37.6 µM	7.71E-7		57	2.05E-6
9	SGTC_43	0570-0105	4.57	72.5 µM	9.46E-7	ERG2	64	2.47E-6
10	SGTC_42	0133-0086	4.20	60.6 µM	5.88E-6		23	1.33E-5
11	SGTC_1090	efavirenz	4.13	20.7 µM	8.29E-6		16	1.83E-5
12	SGTC_52	0329-0193	3.82	875.0 nM	3.31E-5	TSC3-RPN4	39	6.58E-5
13	SGTC_1356	1502-0781	3.69	85.8 µM	5.85E-5	fatty acid desaturase (OLE1)	23	1.11E-4
14	SGTC_1855	st056195	3.68	39.6 µM	6.15E-5	60S ribosome export	24	1.16E-4
15	SGTC_363	0455-0021	3.58	3.1 µM	9.54E-5	azole & statin	83	1.75E-4
16	SGTC_361	000s-0510	3.50	1.4 µM	1.30E-4		51	2.33E-4
17	SGTC_1700	st030840	3.37	7.9 µM	2.22E-4	PDR1	37	3.81E-4
18	SGTC_1932	st074806	3.37	40.9 µM	2.23E-4		49	3.82E-4
19	SGTC_1230	0170-0365	3.26	3.8 µM	3.37E-4	endomembrane recycling	107	5.61E-4
20	SGTC_159	3970-0959	3.25	50.0 µM	3.44E-4		131	5.72E-4

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.133	1.28E-14	YHL016C	DUR3	hom	Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway
0.124	4.88E-13	YGR206W	MVB12	hom	ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin
0.116	1.60E-11	YDL180W_p	YDL180W_p	hom	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
0.112	7.59E-11	YHL023C	NPR3	hom	Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair
0.108	3.28E-10	YDR090C_p	YDR090C_p	hom	Putative protein of unknown function
0.108	4.09E-10	YER109C	FLO8	hom	Transcription factor required for flocculation, diploid filamentous growth, and haploid invasive growth; genome reference strain S288C and most laboratory strains have a mutation in this gene
0.104	1.80E-9	YNL219C	ALG9	hom	Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation
0.102	3.73E-9	YDR516C	EMI2	hom	Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.102	3.92E-9	YBR161W	CSH1	hom	Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication
0.096	2.66E-8	YDR323C	PEP7	hom	Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
0.095	3.80E-8	YDL201W	TRM8	hom	Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p
0.094	4.76E-8	YOL075C_p	YOL075C_p	hom	Putative ABC transporter
0.092	8.61E-8	YMR102C_p	YMR102C_p	hom	Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene
0.091	1.44E-7	YPL120W	VPS30	hom	Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1
0.089	2.49E-7	YNL323W	LEM3	hom	Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane