YDR221W / GTB1

Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YDR221W / GTB1

Click on Significant Values for Screen Details ID:SGTC_42|Compound:0133-0086|FD-Score:4.20|P-value:5.88E-6 ID:SGTC_43|Compound:0570-0105|FD-Score:4.57|P-value:9.46E-7 ID:SGTC_52|Compound:0329-0193|FD-Score:3.82|P-value:3.31E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:-5.72|P-value:1.29E-9 ID:SGTC_159|Compound:3970-0959|FD-Score:3.25|P-value:3.44E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:4.86|P-value:2.03E-7 ID:SGTC_361|Compound:000s-0510|FD-Score:3.50|P-value:1.30E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:3.58|P-value:9.54E-5 ID:SGTC_376|Compound:3970-0862|FD-Score:3.23|P-value:3.70E-4 ID:SGTC_522|Compound:1273-0010|FD-Score:6.66|P-value:1.85E-12 ID:SGTC_529|Compound:4076-0302|FD-Score:-3.35|P-value:2.40E-4 ID:SGTC_600|Compound:1495-0418|FD-Score:-3.03|P-value:7.96E-4 ID:SGTC_706|Compound:4262-0057|FD-Score:-2.98|P-value:9.55E-4 ID:SGTC_1008|Compound:4106-0037|FD-Score:3.05|P-value:7.24E-4 ID:SGTC_1088|Compound:bifonazole|FD-Score:3.13|P-value:5.43E-4 ID:SGTC_1090|Compound:efavirenz|FD-Score:4.13|P-value:8.29E-6 ID:SGTC_1100|Compound:amiodarone|FD-Score:3.11|P-value:5.86E-4 ID:SGTC_1104|Compound:tacrolimus|FD-Score:3.08|P-value:6.46E-4 ID:SGTC_1230|Compound:0170-0365|FD-Score:3.26|P-value:3.37E-4 ID:SGTC_1242|Compound:0402-0103|FD-Score:-3.44|P-value:1.73E-4 ID:SGTC_1246|Compound:0422-0085|FD-Score:-3.18|P-value:4.60E-4 ID:SGTC_1356|Compound:1502-0781|FD-Score:3.69|P-value:5.85E-5 ID:SGTC_1370|Compound:2001-0092|FD-Score:3.04|P-value:7.49E-4 ID:SGTC_1600|Compound:coumarin 106|FD-Score:-3.46|P-value:1.58E-4 ID:SGTC_1621|Compound:st003705|FD-Score:2.99|P-value:8.94E-4 ID:SGTC_1630|Compound:st005209|FD-Score:3.02|P-value:8.19E-4 ID:SGTC_1686|Compound:st019653|FD-Score:3.01|P-value:8.28E-4 ID:SGTC_1700|Compound:st030840|FD-Score:3.37|P-value:2.22E-4 ID:SGTC_1855|Compound:st056195|FD-Score:3.68|P-value:6.15E-5 ID:SGTC_1860|Compound:st056228|FD-Score:4.90|P-value:1.57E-7 ID:SGTC_1932|Compound:st074806|FD-Score:3.37|P-value:2.23E-4 ID:SGTC_2055|Compound:5235184|FD-Score:-3.57|P-value:9.90E-5 ID:SGTC_2518|Compound:prenyletin|FD-Score:5.28|P-value:1.80E-8 ID:SGTC_2526|Compound:patulin|FD-Score:4.61|P-value:7.71E-7 ID:SGTC_2556|Compound:citropten|FD-Score:-3.08|P-value:6.75E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:-3.36|P-value:2.29E-4 ID:SGTC_2874|Compound:9043904|FD-Score:-3.28|P-value:3.14E-4 ID:SGTC_2892|Compound:9047358|FD-Score:-2.98|P-value:9.47E-4 ID:SGTC_2934|Compound:9036370|FD-Score:-3.51|P-value:1.27E-4 ID:SGTC_2937|Compound:9044753|FD-Score:4.73|P-value:4.06E-7 ID:SGTC_3083|Compound:9117465|FD-Score:3.23|P-value:3.69E-4 ID:SGTC_3201|Compound:9113999|FD-Score:4.74|P-value:3.77E-7 ID:SGTC_3209|Compound:9113616|FD-Score:-4.83|P-value:2.47E-7 ID:SGTC_3263|Compound:9137018|FD-Score:-3.36|P-value:2.36E-4 ID:SGTC_3272|Compound:9137413|FD-Score:3.24|P-value:3.59E-4 ID:SGTC_3279|Compound:9140807|FD-Score:-3.55|P-value:1.10E-4 ID:SGTC_3300|Compound:9124615|FD-Score:5.18|P-value:3.25E-8 ID:SGTC_3307|Compound:9126885|FD-Score:-3.31|P-value:2.84E-4 ID:SGTC_42|Compound:0133-0086|FD-Score:4.20|P-value:5.88E-6 ID:SGTC_43|Compound:0570-0105|FD-Score:4.57|P-value:9.46E-7 ID:SGTC_52|Compound:0329-0193|FD-Score:3.82|P-value:3.31E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:-5.72|P-value:1.29E-9 ID:SGTC_159|Compound:3970-0959|FD-Score:3.25|P-value:3.44E-4 ID:SGTC_266|Compound:formosanin C|FD-Score:4.86|P-value:2.03E-7 ID:SGTC_361|Compound:000s-0510|FD-Score:3.50|P-value:1.30E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:3.58|P-value:9.54E-5 ID:SGTC_376|Compound:3970-0862|FD-Score:3.23|P-value:3.70E-4 ID:SGTC_522|Compound:1273-0010|FD-Score:6.66|P-value:1.85E-12 ID:SGTC_529|Compound:4076-0302|FD-Score:-3.35|P-value:2.40E-4 ID:SGTC_600|Compound:1495-0418|FD-Score:-3.03|P-value:7.96E-4 ID:SGTC_706|Compound:4262-0057|FD-Score:-2.98|P-value:9.55E-4 ID:SGTC_1008|Compound:4106-0037|FD-Score:3.05|P-value:7.24E-4 ID:SGTC_1088|Compound:bifonazole|FD-Score:3.13|P-value:5.43E-4 ID:SGTC_1090|Compound:efavirenz|FD-Score:4.13|P-value:8.29E-6 ID:SGTC_1100|Compound:amiodarone|FD-Score:3.11|P-value:5.86E-4 ID:SGTC_1104|Compound:tacrolimus|FD-Score:3.08|P-value:6.46E-4 ID:SGTC_1230|Compound:0170-0365|FD-Score:3.26|P-value:3.37E-4 ID:SGTC_1242|Compound:0402-0103|FD-Score:-3.44|P-value:1.73E-4 ID:SGTC_1246|Compound:0422-0085|FD-Score:-3.18|P-value:4.60E-4 ID:SGTC_1356|Compound:1502-0781|FD-Score:3.69|P-value:5.85E-5 ID:SGTC_1370|Compound:2001-0092|FD-Score:3.04|P-value:7.49E-4 ID:SGTC_1600|Compound:coumarin 106|FD-Score:-3.46|P-value:1.58E-4 ID:SGTC_1621|Compound:st003705|FD-Score:2.99|P-value:8.94E-4 ID:SGTC_1630|Compound:st005209|FD-Score:3.02|P-value:8.19E-4 ID:SGTC_1686|Compound:st019653|FD-Score:3.01|P-value:8.28E-4 ID:SGTC_1700|Compound:st030840|FD-Score:3.37|P-value:2.22E-4 ID:SGTC_1855|Compound:st056195|FD-Score:3.68|P-value:6.15E-5 ID:SGTC_1860|Compound:st056228|FD-Score:4.90|P-value:1.57E-7 ID:SGTC_1932|Compound:st074806|FD-Score:3.37|P-value:2.23E-4 ID:SGTC_2055|Compound:5235184|FD-Score:-3.57|P-value:9.90E-5 ID:SGTC_2518|Compound:prenyletin|FD-Score:5.28|P-value:1.80E-8 ID:SGTC_2526|Compound:patulin|FD-Score:4.61|P-value:7.71E-7 ID:SGTC_2556|Compound:citropten|FD-Score:-3.08|P-value:6.75E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:-3.36|P-value:2.29E-4 ID:SGTC_2874|Compound:9043904|FD-Score:-3.28|P-value:3.14E-4 ID:SGTC_2892|Compound:9047358|FD-Score:-2.98|P-value:9.47E-4 ID:SGTC_2934|Compound:9036370|FD-Score:-3.51|P-value:1.27E-4 ID:SGTC_2937|Compound:9044753|FD-Score:4.73|P-value:4.06E-7 ID:SGTC_3083|Compound:9117465|FD-Score:3.23|P-value:3.69E-4 ID:SGTC_3201|Compound:9113999|FD-Score:4.74|P-value:3.77E-7 ID:SGTC_3209|Compound:9113616|FD-Score:-4.83|P-value:2.47E-7 ID:SGTC_3263|Compound:9137018|FD-Score:-3.36|P-value:2.36E-4 ID:SGTC_3272|Compound:9137413|FD-Score:3.24|P-value:3.59E-4 ID:SGTC_3279|Compound:9140807|FD-Score:-3.55|P-value:1.10E-4 ID:SGTC_3300|Compound:9124615|FD-Score:5.18|P-value:3.25E-8 ID:SGTC_3307|Compound:9126885|FD-Score:-3.31|P-value:2.84E-4

Top fitness defect scores for YDR221W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_522 1273-0010 6.66 11.6 µM 1.85E-12 60S ribosome export 2 1.35E-11
2 SGTC_2518 prenyletin 5.28 46.3 µM 1.80E-8 2 6.41E-8
3 SGTC_3300 9124615 5.18 50.5 µM 3.25E-8 4 1.11E-7
4 SGTC_1860 st056228 4.90 74.6 µM 1.57E-7 4 4.73E-7
5 SGTC_266 formosanin C 4.86 406.0 nM 2.03E-7 sphingolipid biosynthesis & PDR1 50 5.99E-7
6 SGTC_3201 9113999 4.74 49.5 µM 3.77E-7 17 1.06E-6
7 SGTC_2937 9044753 4.73 11.3 µM 4.06E-7 60S ribosome export 18 1.13E-6
8 SGTC_2526 patulin 4.61 37.6 µM 7.71E-7 57 2.05E-6
9 SGTC_43 0570-0105 4.57 72.5 µM 9.46E-7 ERG2 64 2.47E-6
10 SGTC_42 0133-0086 4.20 60.6 µM 5.88E-6 23 1.33E-5
11 SGTC_1090 efavirenz 4.13 20.7 µM 8.29E-6 16 1.83E-5
12 SGTC_52 0329-0193 3.82 875.0 nM 3.31E-5 TSC3-RPN4 39 6.58E-5
13 SGTC_1356 1502-0781 3.69 85.8 µM 5.85E-5 fatty acid desaturase (OLE1) 23 1.11E-4
14 SGTC_1855 st056195 3.68 39.6 µM 6.15E-5 60S ribosome export 24 1.16E-4
15 SGTC_363 0455-0021 3.58 3.1 µM 9.54E-5 azole & statin 83 1.75E-4
16 SGTC_361 000s-0510 3.50 1.4 µM 1.30E-4 51 2.33E-4
17 SGTC_1700 st030840 3.37 7.9 µM 2.22E-4 PDR1 37 3.81E-4
18 SGTC_1932 st074806 3.37 40.9 µM 2.23E-4 49 3.82E-4
19 SGTC_1230 0170-0365 3.26 3.8 µM 3.37E-4 endomembrane recycling 107 5.61E-4
20 SGTC_159 3970-0959 3.25 50.0 µM 3.44E-4 131 5.72E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.133 1.28E-14 YHL016C DUR3 hom Plasma membrane transporter for both urea and polyamines, expression is highly sensitive to nitrogen catabolite repression and induced by allophanate, the last intermediate of the allantoin degradative pathway
0.124 4.88E-13 YGR206W MVB12 hom ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin
0.116 1.60E-11 YDL180W_p YDL180W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the vacuole
0.112 7.59E-11 YHL023C NPR3 hom Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair
0.108 3.28E-10 YDR090C_p YDR090C_p hom Putative protein of unknown function
0.108 4.09E-10 YER109C FLO8 hom Transcription factor required for flocculation, diploid filamentous growth, and haploid invasive growth; genome reference strain S288C and most laboratory strains have a mutation in this gene
0.104 1.80E-9 YNL219C ALG9 hom Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation
0.102 3.73E-9 YDR516C EMI2 hom Non-essential protein of unknown function; required for transcriptional induction of the early meiotic-specific transcription factor IME1; required for sporulation; expression regulated by glucose-repression transcription factors Mig1/2p; EMI2 has a paralog, GLK1, that arose from the whole genome duplication; protein abundance increases in response to DNA replication stress
0.102 3.92E-9 YBR161W CSH1 hom Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Sur1p; CSH1 has a paralog, SUR1, that arose from the whole genome duplication
0.096 2.66E-8 YDR323C PEP7 hom Multivalent adaptor protein that facilitates vesicle-mediated vacuolar protein sorting by ensuring high-fidelity vesicle docking and fusion, which are essential for targeting of vesicles to the endosome; required for vacuole inheritance
0.095 3.80E-8 YDL201W TRM8 hom Noncatalytic subunit of a tRNA methyltransferase complex; Trm8p and Trm82p comprise an enzyme that catalyzes a methyl-transfer from S-adenosyl-l-methionine to the N(7) atom of guanine at position 46 in tRNA; Trm8 lacks catalytic activity if not bound to Trm82p
0.094 4.76E-8 YOL075C_p YOL075C_p hom Putative ABC transporter
0.092 8.61E-8 YMR102C_p YMR102C_p hom Protein of unknown function; transcription is activated by paralogous transcription factors Yrm1p and Yrr1p along with genes involved in multidrug resistance; mutant shows increased resistance to azoles; YMR102C is not an essential gene
0.091 1.44E-7 YPL120W VPS30 hom Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1
0.089 2.49E-7 YNL323W LEM3 hom Membrane protein of the plasma membrane and ER, interacts specifically in vivo with the phospholipid translocase (flippase) Dnf1p; involved in translocation of phospholipids and alkylphosphocholine drugs across the plasma membrane