YDR224C / HTB1

Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR224C / HTB1

Click on Significant Values for Screen Details ID:SGTC_64|Compound:000l-0018|FD-Score:4.44|P-value:4.35E-6 ID:SGTC_127|Compound:0180-0333|FD-Score:-3.14|P-value:9.44E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:4.01|P-value:2.92E-5 ID:SGTC_171|Compound:k064-0041|FD-Score:3.98|P-value:3.42E-5 ID:SGTC_234|Compound:nsc-17383|FD-Score:-3.20|P-value:7.66E-4 ID:SGTC_288|Compound:1315-0019|FD-Score:4.03|P-value:2.72E-5 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:3.42|P-value:3.09E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:3.16|P-value:7.75E-4 ID:SGTC_495|Compound:decylubiquinone|FD-Score:3.66|P-value:1.20E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:4.35|P-value:6.63E-6 ID:SGTC_576|Compound:1073-0113|FD-Score:3.98|P-value:3.33E-5 ID:SGTC_794|Compound:k038-0031|FD-Score:3.44|P-value:2.85E-4 ID:SGTC_802|Compound:4112-3352|FD-Score:3.28|P-value:5.09E-4 ID:SGTC_895|Compound:0964-0006|FD-Score:3.26|P-value:5.30E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:3.34|P-value:4.06E-4 ID:SGTC_1044|Compound:1222-0021|FD-Score:11.60|P-value:2.86E-31 ID:SGTC_1476|Compound:4112-3294|FD-Score:5.39|P-value:3.46E-8 ID:SGTC_1501|Compound:4491-0275|FD-Score:3.20|P-value:6.68E-4 ID:SGTC_1522|Compound:st012842|FD-Score:7.52|P-value:2.92E-14 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:3.99|P-value:3.19E-5 ID:SGTC_1675|Compound:st018485|FD-Score:7.22|P-value:2.63E-13 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:-5.62|P-value:1.22E-8 ID:SGTC_1682|Compound:st012968|FD-Score:3.86|P-value:5.50E-5 ID:SGTC_1718|Compound:roemerine|FD-Score:7.45|P-value:4.94E-14 ID:SGTC_1719|Compound:st032227|FD-Score:-3.39|P-value:4.00E-4 ID:SGTC_1738|Compound:st035555|FD-Score:-4.41|P-value:6.10E-6 ID:SGTC_1744|Compound:st037937|FD-Score:4.79|P-value:8.13E-7 ID:SGTC_1786|Compound:berberine|FD-Score:3.39|P-value:3.40E-4 ID:SGTC_1827|Compound:berberine|FD-Score:4.93|P-value:3.99E-7 ID:SGTC_1829|Compound:berberine|FD-Score:4.32|P-value:7.77E-6 ID:SGTC_2003|Compound:4023581|FD-Score:-3.35|P-value:4.54E-4 ID:SGTC_2066|Compound:5222451|FD-Score:3.53|P-value:2.03E-4 ID:SGTC_2404|Compound:5161160|FD-Score:3.74|P-value:8.79E-5 ID:SGTC_2508|Compound:celastrol|FD-Score:-3.36|P-value:4.42E-4 ID:SGTC_2511|Compound:berberine|FD-Score:4.87|P-value:5.38E-7 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:3.64|P-value:1.33E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:9.11|P-value:4.83E-20 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:-3.27|P-value:5.99E-4 ID:SGTC_2602|Compound:kawain|FD-Score:-3.16|P-value:8.82E-4 ID:SGTC_2623|Compound:humulene|FD-Score:-3.42|P-value:3.52E-4 ID:SGTC_2704|Compound:st078022|FD-Score:3.08|P-value:9.93E-4 ID:SGTC_2759|Compound:disulfiram|FD-Score:4.22|P-value:1.20E-5 ID:SGTC_2762|Compound:leflunomide|FD-Score:3.13|P-value:8.51E-4 ID:SGTC_2821|Compound:7996764|FD-Score:3.17|P-value:7.32E-4 ID:SGTC_2913|Compound:7966556|FD-Score:4.53|P-value:2.86E-6 ID:SGTC_3222|Compound:9128301|FD-Score:3.98|P-value:3.41E-5 ID:SGTC_3296|Compound:9122258|FD-Score:3.25|P-value:5.65E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:4.44|P-value:4.35E-6 ID:SGTC_127|Compound:0180-0333|FD-Score:-3.14|P-value:9.44E-4 ID:SGTC_158|Compound:k038-0087|FD-Score:4.01|P-value:2.92E-5 ID:SGTC_171|Compound:k064-0041|FD-Score:3.98|P-value:3.42E-5 ID:SGTC_234|Compound:nsc-17383|FD-Score:-3.20|P-value:7.66E-4 ID:SGTC_288|Compound:1315-0019|FD-Score:4.03|P-value:2.72E-5 ID:SGTC_402|Compound:epigallocatechin gallate|FD-Score:3.42|P-value:3.09E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:3.16|P-value:7.75E-4 ID:SGTC_495|Compound:decylubiquinone|FD-Score:3.66|P-value:1.20E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:4.35|P-value:6.63E-6 ID:SGTC_576|Compound:1073-0113|FD-Score:3.98|P-value:3.33E-5 ID:SGTC_794|Compound:k038-0031|FD-Score:3.44|P-value:2.85E-4 ID:SGTC_802|Compound:4112-3352|FD-Score:3.28|P-value:5.09E-4 ID:SGTC_895|Compound:0964-0006|FD-Score:3.26|P-value:5.30E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:3.34|P-value:4.06E-4 ID:SGTC_1044|Compound:1222-0021|FD-Score:11.60|P-value:2.86E-31 ID:SGTC_1476|Compound:4112-3294|FD-Score:5.39|P-value:3.46E-8 ID:SGTC_1501|Compound:4491-0275|FD-Score:3.20|P-value:6.68E-4 ID:SGTC_1522|Compound:st012842|FD-Score:7.52|P-value:2.92E-14 ID:SGTC_1592|Compound:4',5'-dihydroxyflavone|FD-Score:3.99|P-value:3.19E-5 ID:SGTC_1675|Compound:st018485|FD-Score:7.22|P-value:2.63E-13 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:-5.62|P-value:1.22E-8 ID:SGTC_1682|Compound:st012968|FD-Score:3.86|P-value:5.50E-5 ID:SGTC_1718|Compound:roemerine|FD-Score:7.45|P-value:4.94E-14 ID:SGTC_1719|Compound:st032227|FD-Score:-3.39|P-value:4.00E-4 ID:SGTC_1738|Compound:st035555|FD-Score:-4.41|P-value:6.10E-6 ID:SGTC_1744|Compound:st037937|FD-Score:4.79|P-value:8.13E-7 ID:SGTC_1786|Compound:berberine|FD-Score:3.39|P-value:3.40E-4 ID:SGTC_1827|Compound:berberine|FD-Score:4.93|P-value:3.99E-7 ID:SGTC_1829|Compound:berberine|FD-Score:4.32|P-value:7.77E-6 ID:SGTC_2003|Compound:4023581|FD-Score:-3.35|P-value:4.54E-4 ID:SGTC_2066|Compound:5222451|FD-Score:3.53|P-value:2.03E-4 ID:SGTC_2404|Compound:5161160|FD-Score:3.74|P-value:8.79E-5 ID:SGTC_2508|Compound:celastrol|FD-Score:-3.36|P-value:4.42E-4 ID:SGTC_2511|Compound:berberine|FD-Score:4.87|P-value:5.38E-7 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:3.64|P-value:1.33E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:9.11|P-value:4.83E-20 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:-3.27|P-value:5.99E-4 ID:SGTC_2602|Compound:kawain|FD-Score:-3.16|P-value:8.82E-4 ID:SGTC_2623|Compound:humulene|FD-Score:-3.42|P-value:3.52E-4 ID:SGTC_2704|Compound:st078022|FD-Score:3.08|P-value:9.93E-4 ID:SGTC_2759|Compound:disulfiram|FD-Score:4.22|P-value:1.20E-5 ID:SGTC_2762|Compound:leflunomide|FD-Score:3.13|P-value:8.51E-4 ID:SGTC_2821|Compound:7996764|FD-Score:3.17|P-value:7.32E-4 ID:SGTC_2913|Compound:7966556|FD-Score:4.53|P-value:2.86E-6 ID:SGTC_3222|Compound:9128301|FD-Score:3.98|P-value:3.41E-5 ID:SGTC_3296|Compound:9122258|FD-Score:3.25|P-value:5.65E-4

Top fitness defect scores for YDR224C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1044 1222-0021 11.60 1.8 µM 2.86E-31 heme biosynthesis & mitochondrial translocase 3 2.18E-31 0.62
2 SGTC_2567 aureobasidin a 9.11 60.0 nM 4.83E-20 14 4.26E-20 0.64
3 SGTC_1522 st012842 7.52 45.6 µM 2.92E-14 RNA pol III & RNase P/MRP 3 2.76E-14 1.66
4 SGTC_1718 roemerine 7.45 11.9 µM 4.94E-14 redox potentiating 5 4.68E-14 0.63
5 SGTC_1675 st018485 7.22 24.5 µM 2.63E-13 60S ribosome export 1 2.51E-13 1.10
6 SGTC_1476 4112-3294 5.39 3.3 µM 3.46E-8 60S ribosome export 1 3.48E-8 1.68
7 SGTC_1827 berberine 4.93 7.2 µM 3.99E-7 60S ribosome export 6 4.05E-7 0.19
8 SGTC_2511 berberine 4.87 10.5 µM 5.38E-7 60S ribosome export 7 5.46E-7 0.07
9 SGTC_1744 st037937 4.79 38.9 µM 8.13E-7 2 8.27E-7 0.40
10 SGTC_2913 7966556 4.53 36.6 µM 2.86E-6 60S ribosome export 4 2.92E-6 0.17
11 SGTC_64 000l-0018 4.44 272.1 µM 4.35E-6 ubiquinone biosynthesis & proteasome 42 4.44E-6 0.11
12 SGTC_525 k781-2675 4.35 26.7 µM 6.63E-6 redox potentiating 36 6.78E-6 0.02
13 SGTC_1829 berberine 4.32 14.4 µM 7.77E-6 60S ribosome export 10 7.96E-6 0.01
14 SGTC_2759 disulfiram 4.22 3.4 µM 1.20E-5 15 1.23E-5 0.02
15 SGTC_288 1315-0019 4.03 35.1 µM 2.72E-5 RSC & ERG11 21 2.79E-5 0.00
16 SGTC_158 k038-0087 4.01 26.4 µM 2.92E-5 ubiquinone biosynthesis & proteasome 32 3.00E-5 0.33
17 SGTC_1592 4',5'-dihydroxyflavone 3.99 8.8 µM 3.19E-5 60S ribosome export 6 3.28E-5 0.07
18 SGTC_576 1073-0113 3.98 22.7 µM 3.33E-5 redox potentiating 44 3.42E-5 0.01
19 SGTC_3222 9128301 3.98 49.5 µM 3.41E-5 60S ribosome export 7 3.50E-5 0.23
20 SGTC_171 k064-0041 3.98 9.7 µM 3.42E-5 heme requiring 15 3.51E-5 0.03

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.261 2.93E-53 YKL004W AUR1 het Phosphatidylinositol:ceramide phosphoinositol transferase (IPC synthase), required for sphingolipid synthesis; can mutate to confer aureobasidin A resistance
0.187 6.76E-28 YBR071W YBR071W hom Protein of unknown function found in the cytoplasm and bud neck; mRNA expression may be regulated by the cell cycle and/or cell wall stress; overexpression of YBR071W affects endocytic protein trafficking
0.177 6.33E-25 YDR367W KEI1 het Component of inositol phosphorylceramide (IPC) synthase; forms a complex with Aur1p and regulates its activity; required for IPC synthase complex localization to the Golgi; post-translationally processed by Kex2p; KEI1 is an essential gene
0.167 2.04E-22 YLR417W VPS36 hom Component of the ESCRT-II complex; contains the GLUE (GRAM Like Ubiquitin binding in EAP45) domain which is involved in interactions with ESCRT-I and ubiquitin-dependent sorting of proteins into the endosome
0.161 7.38E-21 YMR211W DML1 het Essential protein involved in mtDNA inheritance, may also function in the partitioning of the mitochondrial organelle or in the segregation of chromosomes, exhibits regions similar to members of a GTPase family
0.153 4.85E-19 YDR232W HEM1 het 5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p
0.142 1.62E-16 YDL205C HEM3 het Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
0.137 1.67E-15 YKR008W RSC4 het Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p
0.136 2.21E-15 YGL045W RIM8 hom Protein involved in proteolytic activation of Rim101p in response to alkaline pH; interacts with ESCRT-1 subunits Stp22p and Vps28p; essential for anaerobic growth; member of the arrestin-related trafficking adaptor family
0.134 8.22E-15 YGL099W LSG1 het Putative GTPase involved in 60S ribosomal subunit biogenesis; required for the release of Nmd3p from 60S subunits in the cytoplasm
0.133 1.04E-14 YDR086C SSS1 het Subunit of the Sec61p translocation complex (Sec61p-Sss1p-Sbh1p) that forms a channel for passage of secretory proteins through the endoplasmic reticulum membrane, and of the Ssh1p complex (Ssh1p-Sbh2p-Sss1p); interacts with Ost4p and Wbp1p
0.133 1.19E-14 YML014W TRM9 hom tRNA methyltransferase; catalyzes modification of wobble bases in tRNA anticodons to 2, 5-methoxycarbonylmethyluridine and 5-methoxycarbonylmethyl-2-thiouridine; may act as part of a complex with Trm112p; deletion mutation increases translational infidelity, including amino acid misincorporation and -1 frameshifting, and also confers resistance to zymocin; null mutant displays activation of stress responses
0.132 1.68E-14 YDL192W ARF1 hom ADP-ribosylation factor; GTPase of the Ras superfamily involved in regulation of coated vesicle formation in intracellular trafficking within the Golgi; ARF1 has a paralog, ARF2, that arose from the whole genome duplication
0.130 5.00E-14 YMR127C SAS2 hom Histone acetyltransferase (HAT) catalytic subunit of the SAS complex (Sas2p-Sas4p-Sas5p), which acetylates free histones and nucleosomes and regulates transcriptional silencing; member of the MYSTacetyltransferase family
0.126 2.99E-13 YPL065W VPS28 hom Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p