YDR228C / PCF11

mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR228C / PCF11

Click on Significant Values for Screen Details ID:SGTC_102|Compound:gallobenzophenone|FD-Score:4.52|P-value:2.64E-5 ID:SGTC_160|Compound:3453-0671|FD-Score:-3.67|P-value:6.61E-4 ID:SGTC_167|Compound:3672-0051|FD-Score:3.66|P-value:5.10E-4 ID:SGTC_234|Compound:nsc-17383|FD-Score:4.00|P-value:1.70E-4 ID:SGTC_291|Compound:4130-1278|FD-Score:5.16|P-value:1.99E-6 ID:SGTC_305|Compound:1682-2595|FD-Score:3.82|P-value:3.05E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:3.82|P-value:3.02E-4 ID:SGTC_517|Compound:1365-0151|FD-Score:4.63|P-value:1.68E-5 ID:SGTC_584|Compound:0986-0246|FD-Score:3.50|P-value:8.50E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:8.52|P-value:1.59E-14 ID:SGTC_796|Compound:1234-0020|FD-Score:3.48|P-value:8.97E-4 ID:SGTC_807|Compound:1315-0151|FD-Score:3.62|P-value:5.82E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:3.44|P-value:9.91E-4 ID:SGTC_839|Compound:0122-0028|FD-Score:4.33|P-value:5.27E-5 ID:SGTC_997|Compound:3996-0078|FD-Score:5.17|P-value:1.89E-6 ID:SGTC_1004|Compound:4084-0029|FD-Score:3.49|P-value:8.71E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:5.72|P-value:1.61E-7 ID:SGTC_1176|Compound:0848-0131|FD-Score:3.60|P-value:6.08E-4 ID:SGTC_1189|Compound:1431-2119|FD-Score:-4.75|P-value:1.51E-5 ID:SGTC_1201|Compound:1889-3372|FD-Score:5.65|P-value:2.23E-7 ID:SGTC_1202|Compound:1988-0068|FD-Score:4.72|P-value:1.20E-5 ID:SGTC_1203|Compound:3992-3522|FD-Score:4.20|P-value:8.55E-5 ID:SGTC_1213|Compound:1315-0166|FD-Score:5.84|P-value:9.26E-8 ID:SGTC_1214|Compound:0358-0031|FD-Score:4.01|P-value:1.60E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:4.11|P-value:1.17E-4 ID:SGTC_1285|Compound:0929-0069|FD-Score:-5.59|P-value:4.37E-7 ID:SGTC_1320|Compound:1313-0481|FD-Score:4.94|P-value:5.07E-6 ID:SGTC_1496|Compound:4469-0713|FD-Score:3.59|P-value:6.31E-4 ID:SGTC_1524|Compound:corynanthine|FD-Score:4.02|P-value:1.56E-4 ID:SGTC_1563|Compound:conessine|FD-Score:-3.54|P-value:9.76E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:-4.00|P-value:2.26E-4 ID:SGTC_1681|Compound:tripelennamine|FD-Score:3.64|P-value:5.52E-4 ID:SGTC_1745|Compound:st081495|FD-Score:6.29|P-value:1.01E-8 ID:SGTC_1920|Compound:st075728|FD-Score:-4.62|P-value:2.54E-5 ID:SGTC_1923|Compound:st058458|FD-Score:5.88|P-value:7.57E-8 ID:SGTC_1933|Compound:st074711|FD-Score:4.22|P-value:7.73E-5 ID:SGTC_1934|Compound:st074720|FD-Score:5.26|P-value:1.27E-6 ID:SGTC_1948|Compound:st076329|FD-Score:3.63|P-value:5.54E-4 ID:SGTC_1950|Compound:st077106|FD-Score:4.87|P-value:6.60E-6 ID:SGTC_1953|Compound:st076800|FD-Score:4.39|P-value:4.25E-5 ID:SGTC_2192|Compound:5915882|FD-Score:7.06|P-value:1.52E-10 ID:SGTC_2443|Compound:5493264|FD-Score:4.06|P-value:1.37E-4 ID:SGTC_2445|Compound:5742597|FD-Score:4.14|P-value:1.04E-4 ID:SGTC_2447|Compound:5741742|FD-Score:3.64|P-value:5.48E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:-3.97|P-value:2.50E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:4.59|P-value:1.99E-5 ID:SGTC_3001|Compound:9076041|FD-Score:3.55|P-value:7.20E-4 ID:SGTC_3015|Compound:9080845|FD-Score:-3.81|P-value:4.30E-4 ID:SGTC_3123|Compound:9126162|FD-Score:-3.72|P-value:5.56E-4 ID:SGTC_3152|Compound:9099253|FD-Score:4.29|P-value:6.20E-5 ID:SGTC_3186|Compound:9110464|FD-Score:3.49|P-value:8.61E-4 ID:SGTC_3192|Compound:9110362|FD-Score:-3.81|P-value:4.22E-4 ID:SGTC_3210|Compound:9128157|FD-Score:4.33|P-value:5.35E-5 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:4.52|P-value:2.64E-5 ID:SGTC_160|Compound:3453-0671|FD-Score:-3.67|P-value:6.61E-4 ID:SGTC_167|Compound:3672-0051|FD-Score:3.66|P-value:5.10E-4 ID:SGTC_234|Compound:nsc-17383|FD-Score:4.00|P-value:1.70E-4 ID:SGTC_291|Compound:4130-1278|FD-Score:5.16|P-value:1.99E-6 ID:SGTC_305|Compound:1682-2595|FD-Score:3.82|P-value:3.05E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:3.82|P-value:3.02E-4 ID:SGTC_517|Compound:1365-0151|FD-Score:4.63|P-value:1.68E-5 ID:SGTC_584|Compound:0986-0246|FD-Score:3.50|P-value:8.50E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:8.52|P-value:1.59E-14 ID:SGTC_796|Compound:1234-0020|FD-Score:3.48|P-value:8.97E-4 ID:SGTC_807|Compound:1315-0151|FD-Score:3.62|P-value:5.82E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:3.44|P-value:9.91E-4 ID:SGTC_839|Compound:0122-0028|FD-Score:4.33|P-value:5.27E-5 ID:SGTC_997|Compound:3996-0078|FD-Score:5.17|P-value:1.89E-6 ID:SGTC_1004|Compound:4084-0029|FD-Score:3.49|P-value:8.71E-4 ID:SGTC_1133|Compound:3473-0247|FD-Score:5.72|P-value:1.61E-7 ID:SGTC_1176|Compound:0848-0131|FD-Score:3.60|P-value:6.08E-4 ID:SGTC_1189|Compound:1431-2119|FD-Score:-4.75|P-value:1.51E-5 ID:SGTC_1201|Compound:1889-3372|FD-Score:5.65|P-value:2.23E-7 ID:SGTC_1202|Compound:1988-0068|FD-Score:4.72|P-value:1.20E-5 ID:SGTC_1203|Compound:3992-3522|FD-Score:4.20|P-value:8.55E-5 ID:SGTC_1213|Compound:1315-0166|FD-Score:5.84|P-value:9.26E-8 ID:SGTC_1214|Compound:0358-0031|FD-Score:4.01|P-value:1.60E-4 ID:SGTC_1261|Compound:0712-0010|FD-Score:4.11|P-value:1.17E-4 ID:SGTC_1285|Compound:0929-0069|FD-Score:-5.59|P-value:4.37E-7 ID:SGTC_1320|Compound:1313-0481|FD-Score:4.94|P-value:5.07E-6 ID:SGTC_1496|Compound:4469-0713|FD-Score:3.59|P-value:6.31E-4 ID:SGTC_1524|Compound:corynanthine|FD-Score:4.02|P-value:1.56E-4 ID:SGTC_1563|Compound:conessine|FD-Score:-3.54|P-value:9.76E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:-4.00|P-value:2.26E-4 ID:SGTC_1681|Compound:tripelennamine|FD-Score:3.64|P-value:5.52E-4 ID:SGTC_1745|Compound:st081495|FD-Score:6.29|P-value:1.01E-8 ID:SGTC_1920|Compound:st075728|FD-Score:-4.62|P-value:2.54E-5 ID:SGTC_1923|Compound:st058458|FD-Score:5.88|P-value:7.57E-8 ID:SGTC_1933|Compound:st074711|FD-Score:4.22|P-value:7.73E-5 ID:SGTC_1934|Compound:st074720|FD-Score:5.26|P-value:1.27E-6 ID:SGTC_1948|Compound:st076329|FD-Score:3.63|P-value:5.54E-4 ID:SGTC_1950|Compound:st077106|FD-Score:4.87|P-value:6.60E-6 ID:SGTC_1953|Compound:st076800|FD-Score:4.39|P-value:4.25E-5 ID:SGTC_2192|Compound:5915882|FD-Score:7.06|P-value:1.52E-10 ID:SGTC_2443|Compound:5493264|FD-Score:4.06|P-value:1.37E-4 ID:SGTC_2445|Compound:5742597|FD-Score:4.14|P-value:1.04E-4 ID:SGTC_2447|Compound:5741742|FD-Score:3.64|P-value:5.48E-4 ID:SGTC_2592|Compound:piperlongumine|FD-Score:-3.97|P-value:2.50E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:4.59|P-value:1.99E-5 ID:SGTC_3001|Compound:9076041|FD-Score:3.55|P-value:7.20E-4 ID:SGTC_3015|Compound:9080845|FD-Score:-3.81|P-value:4.30E-4 ID:SGTC_3123|Compound:9126162|FD-Score:-3.72|P-value:5.56E-4 ID:SGTC_3152|Compound:9099253|FD-Score:4.29|P-value:6.20E-5 ID:SGTC_3186|Compound:9110464|FD-Score:3.49|P-value:8.61E-4 ID:SGTC_3192|Compound:9110362|FD-Score:-3.81|P-value:4.22E-4 ID:SGTC_3210|Compound:9128157|FD-Score:4.33|P-value:5.35E-5

Top fitness defect scores for YDR228C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_682 0kpi-0099 8.52 17.0 µM 1.59E-14 6 8.02E-18 0.12
2 SGTC_2192 5915882 7.06 198.5 µM 1.52E-10 RNA processing & uracil transport 6 8.34E-13 0.07
3 SGTC_1745 st081495 6.29 74.0 µM 1.01E-8 RNA processing & uracil transport 12 1.63E-10 0.55
4 SGTC_1923 st058458 5.88 19.1 µM 7.57E-8 5 2.06E-9 0.92
5 SGTC_1213 1315-0166 5.84 115.0 µM 9.26E-8 8 2.65E-9 0.68
6 SGTC_1133 3473-0247 5.72 50.0 µM 1.61E-7 RSC complex & mRNA processing 25 5.30E-9 0.02
7 SGTC_1201 1889-3372 5.65 45.9 µM 2.23E-7 Golgi 2 7.97E-9 0.29
8 SGTC_1934 st074720 5.26 32.6 µM 1.27E-6 5 7.06E-8 0.75
9 SGTC_997 3996-0078 5.17 137.0 µM 1.89E-6 2 1.17E-7 0.40
10 SGTC_291 4130-1278 5.16 35.5 µM 1.99E-6 Golgi 3 1.24E-7 0.96
11 SGTC_1320 1313-0481 4.94 95.3 µM 5.07E-6 2 4.00E-7 0.57
12 SGTC_1950 st077106 4.87 7.2 µM 6.60E-6 RNA processing & uracil transport 2 5.57E-7 0.21
13 SGTC_1202 1988-0068 4.72 29.6 µM 1.20E-5 RNA processing & uracil transport 6 1.17E-6 0.22
14 SGTC_517 1365-0151 4.63 113.0 µM 1.68E-5 1 1.79E-6 0.51
15 SGTC_2756 carbadox 4.59 75.1 µM 1.99E-5 13 2.20E-6 0.10
16 SGTC_102 gallobenzophenone 4.52 83.6 µM 2.64E-5 redox potentiating 4 3.14E-6 0.87
17 SGTC_1953 st076800 4.39 12.1 µM 4.25E-5 11 5.69E-6 0.05
18 SGTC_839 0122-0028 4.33 230.0 µM 5.27E-5 RSC complex & mRNA processing 34 7.44E-6 0.09
19 SGTC_3210 9128157 4.33 49.5 µM 5.35E-5 10 7.59E-6 0.01
20 SGTC_3152 9099253 4.29 49.5 µM 6.20E-5 NEO1-PIK1 23 9.12E-6 0.06

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.203 1.43E-32 YML126C ERG13 het 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis
0.176 1.31E-24 YIL104C SHQ1 het Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones
0.155 1.89E-19 YLR424W SPP382 het Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation
0.150 2.34E-18 YCL004W PGS1 het Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis
0.136 3.12E-15 YBL026W LSM2 het Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
0.130 5.33E-14 YMR061W RNA14 het Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
0.128 1.09E-13 YLR014C PPR1 hom Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p
0.128 1.13E-13 YDR419W RAD30 hom DNA polymerase eta, involved in translesion synthesis during post-replication repair; catalyzes the synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; may also have a role in protection against mitochondrial mutagenesis; mutations in human pol eta are responsible for XPV
0.128 1.21E-13 YBR091C TIM12 het Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins
0.124 6.70E-13 YOR236W DFR1 het Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism
0.119 5.73E-12 YCL054W SPB1 het AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants
0.118 6.09E-12 YDR289C RTT103 hom Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition
0.109 2.59E-10 YKL076C_d PSY1_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C
0.108 4.00E-10 YNR012W URK1 hom Uridine/cytidine kinase, component of the pyrimidine ribonucleotide salvage pathway that converts uridine into UMP and cytidine into CMP; involved in the pyrimidine deoxyribonucleotide salvage pathway, converting deoxycytidine into dCMP
0.108 4.25E-10 YNR016C ACC1 het Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;