HIPHOP chemogenomics database

YDR228C / PCF11

mRNA 3' end processing factor, essential component of cleavage and polyadenylation factor IA (CF IA), involved in pre-mRNA 3' end processing and in transcription termination; binds C-terminal domain of largest subunit of RNA pol II (Rpo21p); required for gene looping

Zygosity: Heterozygous strain

fixedexpanded
Profile for YDR228C / PCF11

Click on Significant Values for Screen Details

Top fitness defect scores for YDR228C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_682	0kpi-0099	8.52	17.0 µM	1.59E-14		6	8.02E-18	0.12
2	SGTC_2192	5915882	7.06	198.5 µM	1.52E-10	RNA processing & uracil transport	6	8.34E-13	0.07
3	SGTC_1745	st081495	6.29	74.0 µM	1.01E-8	RNA processing & uracil transport	12	1.63E-10	0.55
4	SGTC_1923	st058458	5.88	19.1 µM	7.57E-8		5	2.06E-9	0.92
5	SGTC_1213	1315-0166	5.84	115.0 µM	9.26E-8		8	2.65E-9	0.68
6	SGTC_1133	3473-0247	5.72	50.0 µM	1.61E-7	RSC complex & mRNA processing	25	5.30E-9	0.02
7	SGTC_1201	1889-3372	5.65	45.9 µM	2.23E-7	Golgi	2	7.97E-9	0.29
8	SGTC_1934	st074720	5.26	32.6 µM	1.27E-6		5	7.06E-8	0.75
9	SGTC_997	3996-0078	5.17	137.0 µM	1.89E-6		2	1.17E-7	0.40
10	SGTC_291	4130-1278	5.16	35.5 µM	1.99E-6	Golgi	3	1.24E-7	0.96
11	SGTC_1320	1313-0481	4.94	95.3 µM	5.07E-6		2	4.00E-7	0.57
12	SGTC_1950	st077106	4.87	7.2 µM	6.60E-6	RNA processing & uracil transport	2	5.57E-7	0.21
13	SGTC_1202	1988-0068	4.72	29.6 µM	1.20E-5	RNA processing & uracil transport	6	1.17E-6	0.22
14	SGTC_517	1365-0151	4.63	113.0 µM	1.68E-5		1	1.79E-6	0.51
15	SGTC_2756	carbadox	4.59	75.1 µM	1.99E-5		13	2.20E-6	0.10
16	SGTC_102	gallobenzophenone	4.52	83.6 µM	2.64E-5	redox potentiating	4	3.14E-6	0.87
17	SGTC_1953	st076800	4.39	12.1 µM	4.25E-5		11	5.69E-6	0.05
18	SGTC_839	0122-0028	4.33	230.0 µM	5.27E-5	RSC complex & mRNA processing	34	7.44E-6	0.09
19	SGTC_3210	9128157	4.33	49.5 µM	5.35E-5		10	7.59E-6	0.01
20	SGTC_3152	9099253	4.29	49.5 µM	6.20E-5	NEO1-PIK1	23	9.12E-6	0.06

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.203	1.43E-32	YML126C	ERG13	het	3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis
0.176	1.31E-24	YIL104C	SHQ1	het	Chaperone protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Naf1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; homology with known Hsp90p cochaperones
0.155	1.89E-19	YLR424W	SPP382	het	Essential protein that forms a dimer with Ntr2p; also forms a trimer, with Ntr2p and Prp43p, that is involved in spliceosome disassembly; found also in a multisubunit complex with the splicing factor Clf1p; suppressor of prp38-1 mutation
0.150	2.34E-18	YCL004W	PGS1	het	Phosphatidylglycerolphosphate synthase, catalyzes the synthesis of phosphatidylglycerolphosphate from CDP-diacylglycerol and sn-glycerol 3-phosphate in the first committed and rate-limiting step of cardiolipin biosynthesis
0.136	3.12E-15	YBL026W	LSM2	het	Lsm (Like Sm) protein; part of heteroheptameric complexes (Lsm2p-7p and either Lsm1p or 8p): cytoplasmic Lsm1p complex involved in mRNA decay; nuclear Lsm8p complex part of U6 snRNP and possibly involved in processing tRNA, snoRNA, and rRNA; relocalizes from nucleus to cytoplasmic foci upon DNA replication stress
0.130	5.33E-14	YMR061W	RNA14	het	Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; bridges interaction between Rna15p and Hrp1p in the CF I complex; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
0.128	1.09E-13	YLR014C	PPR1	hom	Zinc finger transcription factor containing a Zn(2)-Cys(6) binuclear cluster domain, positively regulates transcription of URA1, URA3, URA4, and URA10, which are involved in de novo pyrimidine biosynthesis, in response to pyrimidine starvation; activity may be modulated by interaction with Tup1p
0.128	1.13E-13	YDR419W	RAD30	hom	DNA polymerase eta, involved in translesion synthesis during post-replication repair; catalyzes the synthesis of DNA opposite cyclobutane pyrimidine dimers and other lesions; may also have a role in protection against mitochondrial mutagenesis; mutations in human pol eta are responsible for XPV
0.128	1.21E-13	YBR091C	TIM12	het	Essential protein of the inner mitochondrial membrane, peripherally localized; component of the TIM22 complex, which is a twin-pore translocase that mediates insertion of numerous multispanning inner membrane proteins
0.124	6.70E-13	YOR236W	DFR1	het	Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism
0.119	5.73E-12	YCL054W	SPB1	het	AdoMet-dependent methyltransferase involved in rRNA processing and 60S ribosomal subunit maturation; methylates G2922 in the tRNA docking site of the large subunit rRNA and in the absence of snR52, U2921; suppressor of PAB1 mutants
0.118	6.09E-12	YDR289C	RTT103	hom	Protein that interacts with exonuclease Rat1p and Rai1p and plays a role in transcription termination by RNA polymerase II, has an RPR domain (carboxy-terminal domain interacting domain); also involved in regulation of Ty1 transposition
0.109	2.59E-10	YKL076C_d	PSY1_d	hom	Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C
0.108	4.00E-10	YNR012W	URK1	hom	Uridine/cytidine kinase, component of the pyrimidine ribonucleotide salvage pathway that converts uridine into UMP and cytidine into CMP; involved in the pyrimidine deoxyribonucleotide salvage pathway, converting deoxycytidine into dCMP
0.108	4.25E-10	YNR016C	ACC1	het	Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;