YDR232W / HEM1

5-aminolevulinate synthase, catalyzes the first step in the heme biosynthetic pathway; an N-terminal signal sequence is required for localization to the mitochondrial matrix; expression is regulated by Hap2p-Hap3p

Zygosity: Heterozygous strain
fixedexpanded
Profile for YDR232W / HEM1

Click on Significant Values for Screen Details ID:SGTC_69|Compound:0096-0274|FD-Score:4.78|P-value:1.22E-6 ID:SGTC_82|Compound:0054-0107|FD-Score:-3.33|P-value:5.24E-4 ID:SGTC_171|Compound:k064-0041|FD-Score:15.20|P-value:6.62E-51 ID:SGTC_208|Compound:k072-0098|FD-Score:4.96|P-value:5.24E-7 ID:SGTC_217|Compound:ketoconazole|FD-Score:4.63|P-value:2.57E-6 ID:SGTC_266|Compound:formosanin C|FD-Score:3.17|P-value:8.88E-4 ID:SGTC_284|Compound:0988-0104|FD-Score:8.82|P-value:1.80E-18 ID:SGTC_350|Compound:k064-0020|FD-Score:10.20|P-value:3.12E-24 ID:SGTC_490|Compound:phenamil|FD-Score:8.61|P-value:1.12E-17 ID:SGTC_571|Compound:k831-0106|FD-Score:3.61|P-value:1.88E-4 ID:SGTC_584|Compound:0986-0246|FD-Score:25.20|P-value:9.11E-137 ID:SGTC_602|Compound:3130-1533|FD-Score:4.43|P-value:6.39E-6 ID:SGTC_607|Compound:4491-0986|FD-Score:13.80|P-value:2.80E-42 ID:SGTC_654|Compound:1124-0271|FD-Score:9.20|P-value:6.59E-20 ID:SGTC_842|Compound:0271-0008|FD-Score:4.84|P-value:9.34E-7 ID:SGTC_902|Compound:1120-0002|FD-Score:4.90|P-value:6.91E-7 ID:SGTC_913|Compound:3487-1245|FD-Score:-3.79|P-value:9.52E-5 ID:SGTC_914|Compound:3820-6779|FD-Score:6.13|P-value:7.52E-10 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:11.90|P-value:7.58E-32 ID:SGTC_969|Compound:1313-0238|FD-Score:4.66|P-value:2.22E-6 ID:SGTC_979|Compound:1426-2828|FD-Score:3.37|P-value:4.45E-4 ID:SGTC_1010|Compound:4112-3296|FD-Score:4.30|P-value:1.14E-5 ID:SGTC_1012|Compound:4112-3522|FD-Score:3.71|P-value:1.27E-4 ID:SGTC_1044|Compound:1222-0021|FD-Score:9.78|P-value:2.84E-22 ID:SGTC_1098|Compound:clofazimine|FD-Score:5.62|P-value:1.58E-8 ID:SGTC_1113|Compound:artemisinin|FD-Score:5.47|P-value:3.56E-8 ID:SGTC_1239|Compound:0337-0584|FD-Score:3.20|P-value:8.15E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:5.05|P-value:3.24E-7 ID:SGTC_1290|Compound:0986-0249|FD-Score:4.11|P-value:2.56E-5 ID:SGTC_1310|Compound:1188-0297|FD-Score:4.29|P-value:1.20E-5 ID:SGTC_1346|Compound:1488-2224|FD-Score:3.18|P-value:8.52E-4 ID:SGTC_1556|Compound:plumbagin|FD-Score:4.68|P-value:1.97E-6 ID:SGTC_1932|Compound:st074806|FD-Score:3.19|P-value:8.35E-4 ID:SGTC_1941|Compound:st076221|FD-Score:5.02|P-value:3.84E-7 ID:SGTC_1948|Compound:st076329|FD-Score:3.36|P-value:4.65E-4 ID:SGTC_2044|Compound:5102272|FD-Score:3.67|P-value:1.47E-4 ID:SGTC_2066|Compound:5222451|FD-Score:14.50|P-value:1.57E-46 ID:SGTC_2092|Compound:5303694|FD-Score:3.56|P-value:2.23E-4 ID:SGTC_2124|Compound:5246320|FD-Score:3.93|P-value:5.43E-5 ID:SGTC_2129|Compound:girgensonine|FD-Score:5.00|P-value:4.19E-7 ID:SGTC_2278|Compound:7948257|FD-Score:5.08|P-value:2.84E-7 ID:SGTC_2419|Compound:paclitaxel|FD-Score:-3.39|P-value:4.24E-4 ID:SGTC_2425|Compound:5628681|FD-Score:-3.15|P-value:9.75E-4 ID:SGTC_2444|Compound:5485484|FD-Score:3.28|P-value:6.18E-4 ID:SGTC_2473|Compound:5854593|FD-Score:3.35|P-value:4.77E-4 ID:SGTC_2478|Compound:5763493|FD-Score:-3.60|P-value:1.94E-4 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:3.71|P-value:1.30E-4 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:4.96|P-value:5.01E-7 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.59|P-value:2.01E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:3.92|P-value:5.55E-5 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:4.40|P-value:7.31E-6 ID:SGTC_2739|Compound:tacrine|FD-Score:10.80|P-value:1.51E-26 ID:SGTC_2766|Compound:oltipraz|FD-Score:4.36|P-value:8.59E-6 ID:SGTC_2906|Compound:7926292|FD-Score:-3.54|P-value:2.44E-4 ID:SGTC_2909|Compound:7941780|FD-Score:-4.70|P-value:1.87E-6 ID:SGTC_2928|Compound:9011484|FD-Score:3.52|P-value:2.60E-4 ID:SGTC_2930|Compound:9013230|FD-Score:3.58|P-value:2.13E-4 ID:SGTC_2950|Compound:9072895|FD-Score:3.46|P-value:3.24E-4 ID:SGTC_2953|Compound:9074162|FD-Score:3.88|P-value:6.64E-5 ID:SGTC_3006|Compound:9075424|FD-Score:-4.31|P-value:1.08E-5 ID:SGTC_3009|Compound:9080819|FD-Score:-3.84|P-value:7.65E-5 ID:SGTC_3014|Compound:9081533|FD-Score:-3.52|P-value:2.63E-4 ID:SGTC_3105|Compound:9121040|FD-Score:3.74|P-value:1.13E-4 ID:SGTC_3142|Compound:9086498|FD-Score:3.75|P-value:1.09E-4 ID:SGTC_3180|Compound:9109287|FD-Score:-3.85|P-value:7.48E-5 ID:SGTC_3318|Compound:9139014|FD-Score:3.36|P-value:4.62E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:4.78|P-value:1.22E-6 ID:SGTC_82|Compound:0054-0107|FD-Score:-3.33|P-value:5.24E-4 ID:SGTC_171|Compound:k064-0041|FD-Score:15.20|P-value:6.62E-51 ID:SGTC_208|Compound:k072-0098|FD-Score:4.96|P-value:5.24E-7 ID:SGTC_217|Compound:ketoconazole|FD-Score:4.63|P-value:2.57E-6 ID:SGTC_266|Compound:formosanin C|FD-Score:3.17|P-value:8.88E-4 ID:SGTC_284|Compound:0988-0104|FD-Score:8.82|P-value:1.80E-18 ID:SGTC_350|Compound:k064-0020|FD-Score:10.20|P-value:3.12E-24 ID:SGTC_490|Compound:phenamil|FD-Score:8.61|P-value:1.12E-17 ID:SGTC_571|Compound:k831-0106|FD-Score:3.61|P-value:1.88E-4 ID:SGTC_584|Compound:0986-0246|FD-Score:25.20|P-value:9.11E-137 ID:SGTC_602|Compound:3130-1533|FD-Score:4.43|P-value:6.39E-6 ID:SGTC_607|Compound:4491-0986|FD-Score:13.80|P-value:2.80E-42 ID:SGTC_654|Compound:1124-0271|FD-Score:9.20|P-value:6.59E-20 ID:SGTC_842|Compound:0271-0008|FD-Score:4.84|P-value:9.34E-7 ID:SGTC_902|Compound:1120-0002|FD-Score:4.90|P-value:6.91E-7 ID:SGTC_913|Compound:3487-1245|FD-Score:-3.79|P-value:9.52E-5 ID:SGTC_914|Compound:3820-6779|FD-Score:6.13|P-value:7.52E-10 ID:SGTC_915|Compound:methyl methanesulfonate (MMS)|FD-Score:11.90|P-value:7.58E-32 ID:SGTC_969|Compound:1313-0238|FD-Score:4.66|P-value:2.22E-6 ID:SGTC_979|Compound:1426-2828|FD-Score:3.37|P-value:4.45E-4 ID:SGTC_1010|Compound:4112-3296|FD-Score:4.30|P-value:1.14E-5 ID:SGTC_1012|Compound:4112-3522|FD-Score:3.71|P-value:1.27E-4 ID:SGTC_1044|Compound:1222-0021|FD-Score:9.78|P-value:2.84E-22 ID:SGTC_1098|Compound:clofazimine|FD-Score:5.62|P-value:1.58E-8 ID:SGTC_1113|Compound:artemisinin|FD-Score:5.47|P-value:3.56E-8 ID:SGTC_1239|Compound:0337-0584|FD-Score:3.20|P-value:8.15E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:5.05|P-value:3.24E-7 ID:SGTC_1290|Compound:0986-0249|FD-Score:4.11|P-value:2.56E-5 ID:SGTC_1310|Compound:1188-0297|FD-Score:4.29|P-value:1.20E-5 ID:SGTC_1346|Compound:1488-2224|FD-Score:3.18|P-value:8.52E-4 ID:SGTC_1556|Compound:plumbagin|FD-Score:4.68|P-value:1.97E-6 ID:SGTC_1932|Compound:st074806|FD-Score:3.19|P-value:8.35E-4 ID:SGTC_1941|Compound:st076221|FD-Score:5.02|P-value:3.84E-7 ID:SGTC_1948|Compound:st076329|FD-Score:3.36|P-value:4.65E-4 ID:SGTC_2044|Compound:5102272|FD-Score:3.67|P-value:1.47E-4 ID:SGTC_2066|Compound:5222451|FD-Score:14.50|P-value:1.57E-46 ID:SGTC_2092|Compound:5303694|FD-Score:3.56|P-value:2.23E-4 ID:SGTC_2124|Compound:5246320|FD-Score:3.93|P-value:5.43E-5 ID:SGTC_2129|Compound:girgensonine|FD-Score:5.00|P-value:4.19E-7 ID:SGTC_2278|Compound:7948257|FD-Score:5.08|P-value:2.84E-7 ID:SGTC_2419|Compound:paclitaxel|FD-Score:-3.39|P-value:4.24E-4 ID:SGTC_2425|Compound:5628681|FD-Score:-3.15|P-value:9.75E-4 ID:SGTC_2444|Compound:5485484|FD-Score:3.28|P-value:6.18E-4 ID:SGTC_2473|Compound:5854593|FD-Score:3.35|P-value:4.77E-4 ID:SGTC_2478|Compound:5763493|FD-Score:-3.60|P-value:1.94E-4 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:3.71|P-value:1.30E-4 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:4.96|P-value:5.01E-7 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:3.59|P-value:2.01E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:3.92|P-value:5.55E-5 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:4.40|P-value:7.31E-6 ID:SGTC_2739|Compound:tacrine|FD-Score:10.80|P-value:1.51E-26 ID:SGTC_2766|Compound:oltipraz|FD-Score:4.36|P-value:8.59E-6 ID:SGTC_2906|Compound:7926292|FD-Score:-3.54|P-value:2.44E-4 ID:SGTC_2909|Compound:7941780|FD-Score:-4.70|P-value:1.87E-6 ID:SGTC_2928|Compound:9011484|FD-Score:3.52|P-value:2.60E-4 ID:SGTC_2930|Compound:9013230|FD-Score:3.58|P-value:2.13E-4 ID:SGTC_2950|Compound:9072895|FD-Score:3.46|P-value:3.24E-4 ID:SGTC_2953|Compound:9074162|FD-Score:3.88|P-value:6.64E-5 ID:SGTC_3006|Compound:9075424|FD-Score:-4.31|P-value:1.08E-5 ID:SGTC_3009|Compound:9080819|FD-Score:-3.84|P-value:7.65E-5 ID:SGTC_3014|Compound:9081533|FD-Score:-3.52|P-value:2.63E-4 ID:SGTC_3105|Compound:9121040|FD-Score:3.74|P-value:1.13E-4 ID:SGTC_3142|Compound:9086498|FD-Score:3.75|P-value:1.09E-4 ID:SGTC_3180|Compound:9109287|FD-Score:-3.85|P-value:7.48E-5 ID:SGTC_3318|Compound:9139014|FD-Score:3.36|P-value:4.62E-4

Top fitness defect scores for YDR232W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_584 0986-0246 25.20 15.4 µM 9.11E-137 heme requiring 1 7.29E-141 15.90
2 SGTC_171 k064-0041 15.20 9.7 µM 6.62E-51 heme requiring 1 2.16E-52 4.09
3 SGTC_2066 5222451 14.50 176.0 µM 1.57E-46 heme biosynthesis & mitochondrial translocase 2 6.98E-48 2.45
4 SGTC_607 4491-0986 13.80 66.8 µM 2.80E-42 heme requiring 1 1.67E-43 7.51
5 SGTC_915 methyl methanesulfonate (MMS) 11.90 110.0 mM 7.58E-32 DNA damage response 1 9.37E-33 3.94
6 SGTC_2739 tacrine 10.80 100.9 µM 1.51E-26 heme requiring 1 2.70E-27 4.94
7 SGTC_350 k064-0020 10.20 19.8 µM 3.12E-24 heme requiring 2 6.55E-25 4.10
8 SGTC_1044 1222-0021 9.78 1.8 µM 2.84E-22 heme biosynthesis & mitochondrial translocase 5 6.83E-23 1.98
9 SGTC_654 1124-0271 9.20 80.9 µM 6.59E-20 1 1.87E-20 3.24
10 SGTC_284 0988-0104 8.82 32.6 µM 1.80E-18 heme requiring 1 5.65E-19 2.75
11 SGTC_490 phenamil 8.61 164.0 µM 1.12E-17 heme requiring 1 3.71E-18 3.02
12 SGTC_914 3820-6779 6.13 408.0 µM 7.52E-10 1 4.27E-10 1.07
13 SGTC_1098 clofazimine 5.62 15.1 µM 1.58E-8 heme biosynthesis & mitochondrial translocase 10 9.80E-9 0.03
14 SGTC_1113 artemisinin 5.47 1.2 µM 3.56E-8 heme biosynthesis & mitochondrial translocase 15 2.26E-8 0.15
15 SGTC_2278 7948257 5.08 134.3 µM 2.84E-7 1 1.92E-7 1.02
16 SGTC_1240 0356-0016 5.05 42.1 µM 3.24E-7 mitochondrial response to ROS 2 2.20E-7 0.62
17 SGTC_1941 st076221 5.02 61.8 µM 3.84E-7 sphingolipid biosynthesis & PDR1 5 2.62E-7 0.43
18 SGTC_2129 girgensonine 5.00 121.0 µM 4.19E-7 heme biosynthesis & mitochondrial translocase 14 2.86E-7 0.07
19 SGTC_2541 muurolladie-3-one 4.96 83.1 µM 5.01E-7 excess fatty acid 12 3.44E-7 0.13
20 SGTC_208 k072-0098 4.96 12.4 µM 5.24E-7 heme biosynthesis & mitochondrial translocase 13 3.60E-7 0.53

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.297 1.88E-69 YDL205C HEM3 het Porphobilinogen deaminase, catalyzes the conversion of 4-porphobilinogen to hydroxymethylbilane, the third step in heme biosynthesis; localizes to the cytoplasm and nucleus; expression is regulated by Hap2p-Hap3p, but not by levels of heme
0.291 3.38E-66 YJL167W ERG20 het Farnesyl pyrophosphate synthetase, has both dimethylallyltranstransferase and geranyltranstransferase activities; catalyzes the formation of C15 farnesyl pyrophosphate units for isoprenoid and sterol biosynthesis
0.261 1.67E-53 YDL119C_p YDL119C_p hom Putative mitochondrial transport protein; GFP-fusion protein is induced in response to the DNA-damaging agent MMS; the authentic, non-tagged protein is detected in purified mitochondria
0.253 2.84E-50 YDL118W_d YDL118W_d hom Dubious open reading frame, unlikely to encode a protein; overlaps almost completely with YDL119C; mutations that would affect both YDL118W and YDL119C confer defective telomere maintenance and are synthetically sick or lethal with alpha-synuclein
0.216 1.07E-36 YNL112W DBP2 het ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
0.197 1.36E-30 YBR035C PDX3 hom Pyridoxine (pyridoxamine) phosphate oxidase, has homologs in E. coli and Myxococcus xanthus; transcription is under the general control of nitrogen metabolism
0.176 1.36E-24 YBR198C TAF5 het Subunit (90 kDa) of TFIID and SAGA complexes, involved in RNA polymerase II transcription initiation and in chromatin modification
0.167 1.87E-22 YER021W RPN3 het Essential, non-ATPase regulatory subunit of the 26S proteasome lid, similar to the p58 subunit of the human 26S proteasome; temperature-sensitive alleles cause metaphase arrest, suggesting a role for the proteasome in cell cycle control
0.166 3.27E-22 YGL040C HEM2 het Aminolevulinate dehydratase, a homo-octameric enzyme, catalyzes the conversion of 5-aminolevulinate to porphobilinogen, the second step in heme biosynthesis; enzymatic activity is zinc-dependent; localizes to the cytoplasm and nucleus
0.163 2.16E-21 YBR192W RIM2 het Mitochondrial pyrimidine nucleotide transporter; imports pyrimidine nucleoside triphosphates and exports pyrimidine nucleoside monophosphates; member of the mitochondrial carrier family
0.161 5.82E-21 YDR047W HEM12 het Uroporphyrinogen decarboxylase, catalyzes the fifth step in the heme biosynthetic pathway; localizes to both the cytoplasm and nucleus; a hem12 mutant has phenotypes similar to patients with porphyria cutanea tarda
0.157 5.97E-20 YKR008W RSC4 het Component of the RSC chromatin remodeling complex; found in close proximity to nucleosomal DNA; displaced from the surface of nucleosomal DNA after chromatin remodeling; acetylated (K25) by Gcn5p, altering replication stress tolerance; contains tandem bromodomains that recognize histone H3 acetylated on K14 (H3K14ac) by Gcn5p
0.153 3.83E-19 YGL048C RPT6 het ATPase of the 19S regulatory particle of the 26S proteasome; one of six ATPases of the regulatory particle; involved in the degradation of ubiquitinated substrates; bound by ubiquitin-protein ligases Ubr1p and Ufd4p; localized mainly to the nucleus throughout the cell cycle; protein abundance increases in response to DNA replication stress
0.153 4.85E-19 YDR224C HTB1 het Histone H2B, core histone protein required for chromatin assembly and chromosome function; nearly identical to HTB2; Rad6p-Bre1p-Lge1p mediated ubiquitination regulates transcriptional activation, meiotic DSB formation and H3 methylation
0.151 1.30E-18 YER048W-A ISD11 het Cysteine desulfurase (Nfs1p) activator; essential for the formation of the persulfide intermediate at the desulfurase active site during pyridoxal phosphate-dependent desulfuration of cysteine; required for mitochondrial iron-sulfur cluster biosynthesis